1 is known about the function or functions of
myosin VIIa.
2 that the mutations reduce the duty ratio of
myosin VIIa.
3 d the actin activation of ATPase activity of
myosin VIIa.
4 e activity or the reduction of duty ratio of
myosin VIIa.
5 have cloned cDNAs encoding the rest of human
myosin-VIIa.
6 motifs and the putative coiled coil domain (
myosin VIIa-
5IQ).
7 al tip localization of the recombinant mouse
myosin VIIa-
5IQ-SAH R502P (myoVIIa-sh1) construct.
8 in II promotes its physical interaction with
Myosin VIIa,
a protein responsible for Usher syndrome ty
9 /= 50 muM because of the weak binding of the
myosin VIIa-
ADP-P(i) complex to actin.
10 ents were counterstained using antibodies to
myosin VIIa and calretinin.
11 ell death but led to decreased expression of
myosin VIIa and failure of stereocilia formation.
12 The mouse shaker-1 locus, Myo7a, encodes
myosin VIIA and mutations in the orthologous gene in hum
13 The interactions between
Myosin VIIa and Myosin IIa are conserved in the mammalia
14 y, we identified a novel interaction between
myosin VIIa and PDZD7 by FLAG pull-down assay.
15 Mutations affecting
myosin-VIIa are known to cause deafness and blindness in
16 in-VI is concentrated, and modest amounts of
myosin-VIIa are present.
17 m, which is currently unknown, of activating
myosin VIIA as a cargo-transporting motor.
18 I at 78 hours after gentamicin treatment and
myosin VIIa at 90 hours.
19 the photoreceptor cells of mice with mutant
myosin VIIa by electron immunomicroscopy and microscopic
20 mechanism of the ATPase cycle of Drosophila
myosin VIIA by using a single-headed construct with the
21 The present results suggested that
myosin VIIA can be a processive motor to serve cargo tra
22 In humans, mutations in the gene encoding
myosin VIIa can cause Usher syndrome type 1b (USH1B), a
23 n the MYO7A gene, encoding the motor protein
myosin VIIa,
can cause Usher 1B, a deafness/blindness sy
24 Mutations of
myosin VIIA cause deafness in various species from human
25 eases, and since mutations of unconventional
myosin VIIa cause retinal degeneration, we evaluated myo
26 Defects in
myosin-VIIa cause the shaker-1 phenotype in mice and Ush
27 liary tips, myosin-VI is largely absent, and
myosin-VIIa colocalizes with crosslinks that connect adj
28 f the baculovirus expressed, truncated mouse
myosin VIIa construct containing the head, all 5IQ motif
29 Myosin VIIa-
deficient hair cells showed an abnormally pe
30 vations revealed that full-length Drosophila
myosin VIIA (
DM7A) is a monomer.
31 cells, and similarly enhanced Pou4f3/GFP and
myosin VIIa double-positive cells, when compared to hATO
32 r epithelial ridge cells into Pou4f3/GFP and
myosin VIIa double-positive cells.
33 lls that were different only with respect to
myosin VIIa expression revealed that mutant stereocilia
34 GFP and
myosin VIIa expression was evaluated histologically.
35 In addition, we establish that the two
myosin VIIA FERM repeats share high sequence similarity
36 potential transport and/or anchoring role of
myosin VIIa for USH2 proteins in hair cells.
37 ly inhibited the ATP-induced dissociation of
myosin VIIA from actin.
38 esis approach that allowed the expression of
myosin VIIa from the mouse X chromosome.
39 phenotypes arising from disruption of normal
myosin VIIa function are likely to reflect a loss of ten
40 We found evidence that
myosin VIIa functions in the connecting cilium of each p
41 Mutations in the
myosin VIIa gene (MYO7A) cause a common and severe subty
42 Mutations in the
myosin VIIa gene (MYO7A) cause Usher syndrome type 1B (U
43 Mutations in the human
myosin VIIa gene (MYO7A) have been reported to cause Ush
44 been shown to be caused by mutations in the
myosin VIIa gene (MYO7A) located on 11q14.
45 fness disorder, result from mutations in the
myosin VIIa gene.
46 , was used to determine whether mice without
myosin VIIa had an increased threshold, as assessed by t
47 mutant mice lacking any of the USH1 proteins-
myosin VIIa,
harmonin, cadherin-23, protocadherin-15, sa
48 Myosin VIIa has been identified as the responsible gene
49 These results suggest that
myosin VIIA has slow ATP binding or low affinity for ATP
50 a homology model for the structure of the ck/
myosin VIIA head that indicates myosin VIIAs, like myosi
51 ions in crinkled (ck) disrupt the Drosophila
myosin VIIA heavy chain.
52 Expression is down regulated in maturing (
myosin VIIA immunoreactive) vestibular hair cells and su
53 Mutations in
myosin VIIa in mice can lead to decreased ERG amplitudes
54 , with the aim of elucidating the role(s) of
myosin VIIa in the retina and what might underlie photor
55 Loss of functional
myosin VIIa in the retinal pigment epithelia (RPE) and/o
56 ing a possible clue to the role of mammalian
myosin-VIIa in the inner ear and retina.
57 ecular basis of the dysfunction of USH gene,
myosin VIIa,
in the affected families is unknown to date
58 Our findings suggest that the
myosin VIIa is a "slow", monomeric molecular motor with
59 Although it was originally thought that
myosin VIIA is a dimeric myosin, our electron microscopi
60 It was found that
myosin VIIA is a plus-directed motor.
61 Given that wild-type mouse
myosin VIIa is a slow, high-duty ratio, monomeric motor,
62 Myosin VIIa is also the gene responsible for the inner e
63 Myosin VIIA is an unconventional myosin, responsible for
64 Therefore,
myosin VIIA is classified to be a high duty ratio motor.
65 Myosin VIIa is crucial in hearing and visual processes.
66 hese results suggest that dimer formation of
myosin VIIA is important for its cargo-transporting acti
67 We showed that only
myosin VIIa is indispensable for USH2 complex assembly a
68 However,
myosin VIIa is not required for USH2 complex assembly in
69 These findings suggest that
myosin VIIA is required for normal stereocilia bundle or
70 Myosin VIIa is responsible for tension bearing and the t
71 kinetic basis for the processive motility of
myosin VIIa is the relative magnitude of the release rat
72 Myosin VIIA is therefore required for the normal gating
73 Myosin-VIIa is an unconventional myosin with relatively
74 between adjacent stereocilia indicates that
myosin-VIIa is required for the structural integrity of
75 re of the ck/myosin VIIA head that indicates
myosin VIIAs,
like myosin IIs, have a spectrin-like, SH3
76 Furthermore,
myosin VIIa localized at the tips of all abnormally shor
77 The distinctive features of
myosin VIIa (
long run lengths, slow motility) will be ve
78 oiled-coil domain as well as the full-length
myosin VIIA (
M7full) was expressed.
79 These results suggest that association of
myosin VIIA monomers with membrane via the MyRip/Rab27a
80 fied the effects of USH1B mutations on human
myosin VIIa motor function for the first time.
81 Myosin VIIa moves along actin filaments as a processive,
82 Both
myosin VIIa (
MYO7A) and myosin 1c have been implicated i
83 e head and tail domains of the motor protein
myosin VIIA (
MYO7A) cause deaf-blindness (Usher syndrome
84 undle defects, protocadherin 15 (PCDH15) and
myosin VIIa (
MYO7A), into a common pathway.
85 A presumptive null allele of
myosin VIIa,
Myo7a(4626SB), was used to determine whethe
86 have been identified, encoding connexin-26,
myosin VIIA,
myosin XV, pendrin, otoferlin and alpha-tec
87 Mutations in
myosin VIIA (
myoVIIA), an unconventional myosin, lead to
88 We conclude that
myosin VIIA participates in anchoring and holding membra
89 ed changes in the amino acid sequence of the
myosin VIIa protein, and 1 resulted in a splicing defect
90 Our results strongly suggest that
myosin VIIa regulates the establishment of a setpoint fo
91 Myosin VIIA required an extremely high ATP concentration
92 Myosin VIIa,
required for adaptation of hair cell mechan
93 The results suggest that Drosophila
myosin VIIA spends the majority of the ATPase cycle in a
94 Drosophila
myosin VIIA,
the homolog of the human Usher Syndrome 1B
95 Myosin VIIA,
thought to be involved in human auditory fu
96 In cells, this kinetic behavior would allow
myosin VIIa to exert and hold tension on actin filaments
97 AB27b mobilizes the effector MyRIP and motor
Myosin VIIa to mediate bacterial expulsion.
98 ression of MyRip promoted the association of
myosin VIIA to vesicles and the dimer formation.
99 The forced dimer of
myosin VIIA translocated its cargo molecule, MyRip, to t
100 cargo molecule, functions as an activator of
myosin VIIA transporter function.
101 study examined the changes of myosin VI and
myosin VIIa,
two unconventional myosins that are critica
102 at a gene encoding an unconventional myosin,
myosin VIIA,
underlies the mouse recessive deafness muta
103 teract in vitro with USH1 proteins including
myosin VIIa,
USH1G (SANS), CIB2 and harmonin.
104 tudied the molecular mechanism of Drosophila
myosin VIIa,
using transient kinetics and single-molecul
105 Myosin VIIA was cloned from rat kidney, and the construc
106 Expression of GFP and
myosin VIIa was confirmed in the RPE and photoreceptors
107 The directionality of
myosin VIIA was determined by using the polarity-marked
108 Part of the gene encoding human
myosin VIIa was found in the preliminary cosmid contig.
109 Myosin VIIa was not expressed in the new hair cells unti
110 Human
myosin VIIA was present throughout cytosol, but it moved
111 cilitates the cargo-transporting activity of
myosin VIIA,
which is achieved by cluster formation on t
112 ied the molecular mechanism of regulation of
myosin VIIA,
which is currently unknown.
113 ule, MyRip, to the tip of filopodia, whereas
myosin VIIA without the forced dimer-forming module does
114 On the other hand,
myosin VIIA without the forced dimerization module becam