ライフサイエンスコーパス: myosin subfragment-1

1 ring the first turnover (based on 180 microM myosin subfragment 1).

2 ubstituted it for the VELC of bovine cardiac myosin subfragment 1.

3 n either MgADP.BeFx.SlDc or chicken skeletal myosin subfragment 1.

4 ore typical, long filaments upon addition of myosin subfragment 1.

5 , and to a lesser degree, thin filament with myosin subfragment 1.

6 t various times after rapidly mixing ATP and myosin subfragment 1.

7 th nucleotide binding to the skeletal muscle myosin subfragment-1.

8 ures are similar to that of chicken skeletal myosin subfragment-1.

9 e binding of actin to rabbit skeletal muscle myosin subfragment-1 (a single-headed subfragment) can b

10 Myosin subfragment 1 and a series of nucleoside triphosp

11 omic model of acto-S1, the complex formed by myosin subfragment 1 and actin, reveals that the regulat

12 ansfer between the regulatory light chain on myosin subfragment-1 and the C-terminus of actin was mea

13 nd the C-terminal long alpha-helix domain of myosin subfragment 1 as well as myosin subfragment 2 (Gl

14 Fitting the acto-myosin subfragment 1 atomic structure into the tomogram

15 It inhibits acto-myosin subfragment 1 ATPase (acto-S-1 ATPase) and filame

16 CA(2+)-sensitive regulation of thin filament-myosin subfragment 1 ATPase activity, or the CA2+ concen

17 onin T inhibited actin-tropomyosin-activated myosin subfragment-1 ATPase activity to the same extent

18 actin and WT troponin and beta-TM, activated myosin subfragment-1 ATPase in a calcium-dependent, coop

19 beta-cardiac myosin subfragment 1 (betaS1) tertiary structure and dyn

20 was 41 s-1 in the first turnover based on a myosin subfragment 1 concentration of 150 microM.

21 tion greatly weakened tropomyosin binding to myosin subfragment 1-decorated actin, with the full-leng

22 er with the reconstituted thin filament, but myosin subfragment 1 decreased the transfer, consistent

23 plakinolide-induced filaments decorated with myosin subfragment 1, demonstrating unequivocally that t

24 nylyl-imidodiphosphate) binding to wild-type myosin subfragment-1 enhanced tryptophan fluorescence by

25 ulation of the interaction between actin and myosin subfragment 1: evidence for three states of the t

26 Both C0 and C1 compete with myosin subfragment 1 for binding to F-actin and effectiv

27 Myosin subfragment 1-fragment decoration demonstrated th

28 We studied the regulation of fluorescent myosin subfragment 1 (fS1) binding to rigor myofibrils o

29 e incubated with a wide range of fluorescent myosin subfragment 1(fS1) at pCa 9 or pCa 4 with or with

30 Acanthamoeba myosin II, heavy meromyosin and myosin subfragment 1, have actin-activated MgATPase that

31 Myosin subfragment 1 hydrophobicity was found to be sens

32 -ATP (1b, enf-ATP) to act as a substrate for myosin subfragment 1 in the presence and absence of acti

33 cs of their interaction with rabbit skeletal myosin subfragment 1 in the presence and absence of acti

34 ation of myosin observed in chicken skeletal myosin subfragment-1 is unable to hydrolyze ATP and most

35 mation observed here and in chicken skeletal myosin subfragment-1 is unable to hydrolyze ATP and repr

36 We expressed and purified human beta-cardiac myosin subfragment 1 (M2beta-S1) containing a C-terminal

37 I-troponin was inhibitory in a thin filament-myosin subfragment 1 MgATPase assay.

38 he tropomyosin deletion suppressed the actin-myosin subfragment 1 MgATPase rate and the in vitro slid

39 tation resulted in a decreased thin filament-myosin subfragment 1 MgATPase rate.

40 The force-extension curve of single myosin subfragment-1 molecules, interacting in the rigor

41 While mutations in the myosin subfragment 1 motor domain can directly disrupt t

42 ce and presence of N-ethylmaleimide-modified myosin subfragment 1 (NEM-S1) at both short and long SLs

43 The binding of N-ethylmaleimide-treated myosin subfragment 1 (NEM-S1) to actin filaments mimics

44 f fibers with a strong binding derivative of myosin subfragment 1 (NEM-S1).

45 -binding, non-force-generating derivative of myosin subfragment-1 (NEM-S1) in chemically skinned myoc

46 activation with Ca(2+) and N-ethyl-maleimide myosin subfragment-1 (NEM-S1), a non-tension-generating,

47 fter addition of N-ethylmaleimide-conjugated myosin subfragment-1 (NEM-S1), a strongly binding myosin

48 trong-binding, nonforce-generating analog of myosin subfragment-1 (NEM-S1).

49 onship when actin filaments are propelled by myosin subfragment 1 or full-length myosin.

50 Superposition of the structures of ncd and myosin subfragment 1 reveals that the labeled cysteine i

51 striated muscle contraction, the effects of myosin subfragment 1 (S-1) on the actin binding of cardi

52 erimental data on the equilibrium binding of myosin subfragment 1 (S-1) to regulated actin filaments

53 measurements of the binding stoichiometry of myosin subfragment-1 (S-1) to the RTF.

54 arent KM or the Vmax for MgATP hydrolysis by myosin subfragment 1 (S1) alone, nor did it affect the v

55 AP-actin binds to skeletal myosin subfragment 1 (S1) and forms a homogeneous comple

56 y bound to single-headed fragments of muscle myosin subfragment 1 (S1) and non-muscle myosin V (MV).

57 intensity difference between rabbit skeletal myosin subfragment 1 (S1) and nucleotide-bound or trappe

58 tional similarity to the extensively studied myosin subfragment 1 (S1) and their accessibility to mol

59 Myosin subfragment 1 (S1) and tropomyosin inhibited the

60 The regulation of myosin subfragment 1 (S1) binding to actin by Tm has bee

61 ionic strength dependence of skeletal muscle myosin subfragment 1 (S1) binding to actin in the presen

62 model predict very similar binding traces of myosin subfragment 1 (S1) binding to regulated actin fil

63 active site (near or at Trp 130) of skeletal myosin subfragment 1 (S1) by using luminescence resonanc

64 ) and SH2 (Cys697) groups on rabbit skeletal myosin subfragment 1 (S1) can be cross-linked by using r

65 Myosin subfragment 1 (S1) can be specifically modified a

66 in (Tn), and weakens Tm binding to the actin-myosin subfragment 1 (S1) complex (acto-S1).

67 The reactive SH1 (Cys-707) group of the myosin subfragment 1 (S1) has been used frequently as an

68 Past biochemical work on myosin subfragment 1 (S1) has shown that the bent alpha-

69 The conformation of myosin subfragment 1 (S1) in the vicinity of the ATP sen

70 510 (Trp510) fluorescence in rabbit skeletal myosin subfragment 1 (S1) indicates the conformation of

71 premixing actin with the NA3 prior to adding myosin subfragment 1 (S1) inhibits the rate of actoS1 as

72 Surmises of how myosin subfragment 1 (S1) interacts with actin filaments

73 n of the reactive lysine (Lys84) in skeletal myosin subfragment 1 (S1) introduces a chiral probe (TNP

74 When smooth muscle myosin subfragment 1 (S1) is bound to actin filaments in

75 The Mg-ATPase activity of skeletal muscle myosin subfragment 1 (S1) is reversibly eliminated when

76 Myosin subfragment 1 (S1) is the ATP catalyzing motor pr

77 The myosin subfragment 1 (S1) MgATPase rate was measured usi

78 o result from an angular displacement of the myosin subfragment 1 (S1) tail domain with respect to th

79 Here, we show that the addition of myosin subfragment 1 (S1) to TFs reconstituted with thes

80 tudied in the absence and in the presence of myosin subfragment 1 (S1) using multifrequency phase/mod

81 ilon-ADP) release from cleaved and uncleaved myosin subfragment 1 (S1) was examined.

82 Lys-553 of skeletal muscle myosin subfragment 1 (S1) was specifically labeled with

83 Tm) on ATPase and on the binding kinetics of myosin subfragment 1 (S1) were studied to clarify the me

84 The interaction of myosin subfragment 1 (S1) with actin-tropomyosin-troponi

85 ns of Cys-697 and Cys-707 of skeletal muscle myosin subfragment 1 (S1) with N,N'-p-phenylenedimaleimi

86 Myosin subfragment 1 (S1) with SH1 (Cys(707)) and SH2 (C

87 n was used for monitoring the interaction of myosin subfragment 1 (S1) with the His-40-Gly-42 site in

88 yosin was studied by comparing the effect of myosin subfragment 1 (S1) with two other structural pert

89 For myosin subfragment 1 (S1), the degree of lever arm rotat

90 psin made it possible to isolate homogeneous myosin subfragment 1 (S1), uncontaminated by endogenous

91 (2+) concentration conditions: inhibition of myosin subfragment 1 (S1)-thin filament MgATPase activit

92 Labeled RLC was reconstituted onto myosin subfragment 1 (S1).

93 nd Cys(697) (SH2) in the catalytic domain of myosin subfragment 1 (S1).

94 anges in actin that are then communicated to myosin subfragment 1 (S1).

95 along with R403Q, to facilitate isolation of myosin subfragment 1 (S1).

96 king of known atomic structures of actin and myosin subfragment 1 (S1; the head and neck region of my

97 anine reduced the strong binding of actin to myosin subfragment-1 (S1) 9-fold compared to wild-type a

98 Atomic models of myosin subfragment-1 (S1) and the actin filament are doc

99 Neither acto-myosin subfragment-1 (S1) ATPase activity nor the trypti

100 When myosin subfragment-1 (S1) binds to actin, these lysines

101 calcium affinity in regulated thin filament-myosin subfragment-1 (S1) MgATPase assays, a 20% decreas

102 ng constant for DNEQ and delta-DSE actins to myosin subfragment-1 (S1) relative to that of wild type

103 The proximity of skeletal myosin subfragment-1 (S1) to actin, and its orientation

104 The binding curve of myosin subfragment-1 (S1) to F-actin is not a simple hyp

105 Myosin subfragment-1 (S1) was labeled with NPM in the pr

106 mide, and the labeled ELC was exchanged into myosin subfragment-1 (S1).

107 Purified wild-type and mutant myosin subfragments 1 (S1), expressed in Dictyostelium,

108 Atomic structures of scallop myosin subfragment 1(S1) with the bound MgADP, MgAMPPNP,

109 Irradiation of the stable myosin subfragment 1(S1).MgADP.orthovanadate (Vi) comple

110 In analogy to SH1-labeled myosin subfragment 1 (SH1-S1), SH2-labeled S1 (SH2-S1) a

111 , MgADP, and smooth muscle (chicken gizzard) myosin subfragment 1 (smS1).

112 dues had no effect on Ca2+-activation of the myosin subfragment 1-thin filament MgATPase rate and did

113 tropomyosin to actin, cooperative binding of myosin subfragment 1 to the thin filament, CA(2+)-sensit

114 cooperativity in the equilibrium binding of myosin subfragment 1, to actin but the binding curves di

115 The interaction with skeletal myosin subfragment 1 was measured in the absence and pre

116 In contrast to rabbit skeletal myosin subfragment 1, where the mant group is protected

117 e examined the interactions of smooth muscle myosin subfragment 1 with ADP to see if this additional