ライフサイエンスコーパス: myristoylation

1 be modified by lysine fatty acylation (e.g. myristoylation).

2 atty acid myristate to protein substrates (N-myristoylation).

3 ARL13b stability, which is not observed with myristoylation.

4 of NPHP3 and found the interaction requires myristoylation.

5 K(+) channel (hSlo1) might undergo internal myristoylation.

6 ter mobility of cytosolic form is due to its myristoylation.

7 required for membrane binding, possibly via myristoylation.

8 the ciliary base was dependent on N-terminal myristoylation.

9 sibility that Src might also be regulated by myristoylation.

10 dimer in solution independent of Ca(2+) and myristoylation.

11 lso depend on anchoring of the N terminus by myristoylation.

12 erminus and the other that is deficient in N-myristoylation.

13 calized to the plasma membrane by N-terminal myristoylation.

14 It also required HMG-coenzyme A and myristoylation.

15 he N-terminal domain of Nef encompassing its myristoylation.

16 h cGKII also associating with the BB via its myristoylation.

17 endent manner, but this effect also requires myristoylation.

18 logical activity of NCS-1 is governed by its myristoylation.

19 this interaction did not require N-terminal myristoylation.

20 FA in promoting oocyte fate through protein myristoylation.

21 critical for the infectivity of VACV and for myristoylation.

22 us of Arf and was largely independent of Arf myristoylation.

23 mutants, with changes at A4 and A5, undergo myristoylation.

24 d by posttranslational modifications such as myristoylation.

25 ved in all poxviruses: a site for N-terminal myristoylation, 14 cysteines, and a C-terminal transmemb

26 Myristoylation, a 14-carbon lipid, is shown to largely s

27 ncated VP4 capsid protein lacking N-terminal myristoylation, a carboxyl-terminal pX extension of VP1,

28 Loss of myristoylation abolished the tumorigenic potential of Sr

29 Block of myristoylation abolished these effects, leaving regulati

30 y shift assays of ligand binding and peptide-myristoylation activity in scintillation proximity assay

31 hymus represents an organ with a prominent N-myristoylation activity.

32 Myristoylation affects both the rate and intensity of re

33 l study of the molecular mechanisms by which myristoylation affects protein folding and function, whi

34 channels and regulate them like CaM, whereas myristoylation allows differential, Ca2+-independent reg

35 Myristoylation also has almost no effect on protein fold

36 tifs for both endoproteolytic cleavage and N-myristoylation, although the function of these post-tran

37 Two recent papers show that, rather than myristoylation, amino-terminal acetylation of the Arf-li

38 es with membranes via the combined action of myristoylation and a polybasic effector domain, which bi

39 cid (C14), a principal substrate for protein myristoylation and a potential peroxisomal beta-oxidatio

40 n this report, we investigated whether the N-myristoylation and Ca(2+)-binding domains of CHP3 are im

41 llular fractionation experiments showed that myristoylation and Ca2+ binding by Ncs1p are essential f

42 s reversible phase behavior is retained upon myristoylation and can be tuned to span a 30-60 degrees

43 SVIP is anchored to microsomal membrane via myristoylation and co-fractionated with gp78, Derlin1, p

44 lly overlapping Noonan syndrome, promoting N-myristoylation and constitutive targeting of the mutant

45 changes, and determine structural effects of myristoylation and dimerization.

46 dopsis and rice lack a transmembrane domain, myristoylation and GPI-anchor protein modifications.

47 We also demonstrate that myristoylation and membrane binding regulate c-Src ubiqu

48 t this property of GPC is independent of its myristoylation and of coexpression with the virus matrix

49 Myristoylation and palmitoylation are critical for paras

50 In contrast, HGAL myristoylation and palmitoylation avert its inhibitory e

51 that membrane targeting of v3 is mediated by myristoylation and palmitoylation of its N-terminal MGC

52 Myristoylation and palmitoylation of the N terminus of F

53 This was validated by direct myristoylation and palmitoylation studies, which indicat

54 Fatty acylation, such as myristoylation and palmitoylation, targets proteins to c

55 nuclear/Golgi region by virtue of N-terminal myristoylation and palmitoylation.

56 scopic methods, we analyzed the effects of N-myristoylation and phosphorylation at Ser10 on the inter

57 Point mutations abolishing either Neurl1 myristoylation and plasma membrane localization or Neurl

58 y abolished PM localization, suggesting that myristoylation and possibly the N-terminal domain contri

59 ry and that the protein does not contain the myristoylation and prenylation lipid-anchoring motifs th

60 t demonstration of a functional role for CNB myristoylation and reveal the importance of Nmt1 in modu

61 in caveolae and is dually acylated by both N-myristoylation and S-palmitoylation.

62 B13 exposure blocked Src myristoylation and Src localization to the cytoplasmic m

63 tituted for functional PKGI, suggesting that myristoylation and subsequent membrane association block

64 Together our results indicate that both the myristoylation and the CHCH domain are essential for the

65 , we systematically analyzed the role of the myristoylation and the CHCH domain in the import and mit

66 We concluded that myristoylation and the pocket residues regulate c-Src, b

67 he localization of BBLF1 at the TGN requires myristoylation and two acidic clusters, which interact w

68 This is the first report linking N-myristoylation and ubiquitin-proteasome-dependent proteo

69 spase-3-cleaved fragment (post-translational myristoylation) and that myristoylation of this fragment

70 g and activation, which are dependent on its myristoylation, and block JNK activation.

71 ading frame, yielding a potential site for N myristoylation, and that mutation of the critical glycin

72 Here, we show that CNB myristoylation antagonizes phosphatase activation in yea

73 effects of folding and function arising from myristoylation are profoundly different from the effects

74 and that the oncogenic effects conferred by myristoylation arise, in part, from the tendency of the

75 changes, and evaluate structural effects of myristoylation as studied by isothermal titration calori

76 via lipid modifications (palmitoylation and myristoylation) as opposed to membrane spanning domains.

77 trated that this association was mediated by myristoylation at Gly(2) and palmitoylation at Cys(3).

78 rescent protein fusions, we demonstrate that myristoylation at glycine 2 and an acidic cluster (AC; a

79 a dual-specificity kinase in vitro and that myristoylation at its amino terminus promotes associatio

80 Myristoylation at the N terminus alters how calmodulin b

81 MST1 to the membrane, either through NORE or myristoylation, augments the apoptotic efficacy of MST1.

82 mbrane localization of TRAM is the result of myristoylation because mutation of a predicted myristoyl

83 McCPK1 undergoes myristoylation, but not palmitoylation in vitro.

84 rom mutation-function analysis reveal that Z myristoylation, but not the Z late (L) or RING domain, i

85 1 and 3 with alanines decreased hSlo1 direct myristoylation by 40-44%, whereas in combination decreas

86 Disruption of CNB myristoylation by mutation of the myristoylated glycine

87 by 40-44%, whereas in combination decreased myristoylation by nearly 90% and abolished the myristic

88 Our results indicate that although N-myristoylation causes no spectroscopically discernible c

89 The SLAP-2 protein contains an NH2-terminal myristoylation consensus sequence and SH3 and SH2 Src ho

90 sharing N-terminal sequence homology, lack a myristoylation consensus sequence.

91 t cannot be mimicked in vivo by heterologous myristoylation consensus sequences.

92 Multiple KEPI N-terminal myristoylation consensus sites provide potential regions

93 To determine whether myristoylation contributes to their distinctive modulato

94 On expression of myristoylation-defective (G2A) NKD2, neither NKD2 nor TG

95 arly, in vitro translated NCS-1, but not its myristoylation-defective mutant, was found associated wi

96 i) kinase activation was not detected with a myristoylation-defective Nef (HIV-1(SF2)NefG2A) or with

97 When N-myristoylation-defective rapsyn-EGFP mutant (G2A) and RI

98 In Aspergillus nidulans, the N-myristoylation deficient swoF1 mutant was previously sho

99 -Darby canine kidney (MDCK) cells expressing myristoylation-deficient (G2A) Naked2.

100 ells expressing eNOS alone or ERalpha plus a myristoylation-deficient mutant eNOS were insensitive.

101 A myristoylation-deficient mutant of the Fus1 protein abro

102 ion factor-like 3 (Arl3) in a nucleotide and myristoylation-dependant manner.

103 rinuclear immunostaining pattern, indicating myristoylation dependent association with nonmitochondri

104 of the myristoylated c-Src peptide are both myristoylation-dependent and sequence-specific.

105 ntify an unexpected function for NKD2, i.e., myristoylation-dependent escort of TGF alpha to the baso

106 These data suggest a possible myristoylation-dependent function of MARCKS to inhibit c

107 o associate with membranes increases in an N-myristoylation-dependent manner, which is suggestive of

108 h the plasma membrane and Golgi complex in a myristoylation-dependent manner.

109 Differential, myristoylation-dependent regulation of presynaptic Ca2+

110 g energetics or NMR spectra, suggesting that myristoylation does not influence the structure of the m

111 By contrast, the myristoylation domain of MARCKS needed for membrane asso

112 G2A mutant Z proteins by the addition of the myristoylation domain of the tyrosine protein kinase Src

113 Global quantification of N-myristoylation during normal growth or apoptosis allowed

114 We also assessed the myristoylation efficiency of the mutants and their abili

115 Protein N-myristoylation enables localization to membranes and hel

116 Eukaryotes have conserved N-myristoylation enzymes, involving one or two N-myristoyl

117 show that in contrast to the case for c-Abl, myristoylation exerts a positive effect on c-Src kinase

118 sing a hippocalcin mutant lacking N-terminal myristoylation exhibit a small I(sAHP) that is similar t

119 In these pathways, N-myristoylation facilitates association of substrate prot

120 To elucidate the role of protein N-myristoylation for thymocyte development, we generated m

121 Mutation of residues essential for either N-myristoylation (G2A) or calcium binding (D123A) did not

122 Alanine substitutions of predicted myristoylation (glycine-2) and palmitoylation (cysteine-

123 Furthermore, we showed that the absence of myristoylation had an immunosuppressive effect on T cell

124 Myristoylation has been shown to be important for the me

125 the Ca(2+)-free and Ca(2+)-bound states, and myristoylation has no effect on protein structure and fo

126 lin-1 is abrogated; the same peptide lacking myristoylation has no inhibitory activity.

127 Mutations that abolish SSP myristoylation have been shown to reduce pH-induced cell

128 xcept the first 34 amino acids important for myristoylation highly compromised the ability to transfo

129 n repertoire of co- and post-translational N-myristoylation in addition to validating tools for the p

130 provide important functional insight into N-myristoylation in plants by ascribing postembryonic func

131 These results support a role of N-myristoylation in the allosteric regulation of PKA-C.

132 se reverse genetics to study the role of SSP myristoylation in the context of the intact virion.

133 In order to examine the role of SSP myristoylation in the context of the intact virus, we us

134 chemical biology approach to explore protein myristoylation in the major human parasite P. falciparum

135 e pocket of the enzyme and inhibit protein N-myristoylation in trypanosomes.

136 e currently no methods for reversing protein myristoylation in vivo.

137 esponse when compared to immunization with a myristoylation-incompetent version of the construct.

138 the protein to acidic membrane surfaces, and myristoylation increases the affinity by a factor of 10;

139 Myristoylation increases the barely detectable intrinsic

140 hermal titration calorimetric data show that myristoylation increases the degree of cooperativity; th

141 Among those, N-myristoylation increases the kinase affinity for membran

142 lar localization of Pto was independent of N-myristoylation, indicating that N-myristoylation is requ

143 These results suggest that myristoylation influences the protein conformation and C

144 Likewise, treatment with the myristoylation inhibitor 2-hydroxymyristic acid inhibite

145 ical proof of concept for the use of protein myristoylation inhibitors as a strategy to block prostat

146 Protein N-myristoylation is a 14-carbon fatty-acid modification th

147 N-myristoylation is a co-translational modification occurr

148 N-myristoylation is a crucial irreversible eukaryotic lipi

149 Protein myristoylation is a means by which cells anchor proteins

150 N-myristoylation is a protein lipidation event in which my

151 N-myristoylation is a protein modification process in whic

152 Therefore, Pto myristoylation is a quantitative factor for effector rec

153 Protein N-myristoylation is a ubiquitous co- and post-translationa

154 N-myristoylation is an essential fatty acid modification t

155 Collectively, the data suggest that myristoylation is an important structural determinant of

156 Protein N-myristoylation is catalysed by N-myristoyltransferase (N

157 Although N-terminal myristoylation is catalyzed co-translationally within th

158 Myristoylation is critical for membrane association of S

159 antitative dose response for inhibition of N-myristoylation is determined for >70 substrates simultan

160 Therefore, protein myristoylation is indispensable in T cell development an

161 ate that AtCPK2 is localized to the ER, that myristoylation is likely to be involved in the membrane

162 ranslocate to membranes, and the function of myristoylation is not established.

163 Our results show that myristoylation is required for Fus1-mediated tumor-suppr

164 myristoylated at its amino terminus and that myristoylation is required for membrane binding.

165 ndent of N-myristoylation, indicating that N-myristoylation is required for some function other than

166 phosphorylated form of pp28 is incorporated, myristoylation is required, and sequences within the fir

167 N-myristoylation is the covalent attachment of myristic ac

168 N-myristoylation is the irreversible attachment of a C(14)

169 e identity of the enzyme catalyzing internal myristoylation is unknown.

170 ivo, and show that selective inhibition of N-myristoylation leads to catastrophic and irreversible fa

171 These results suggest that SSP myristoylation may function late in the fusion process t

172 n by ULK1 was dependent on AMPK beta-subunit myristoylation, metabolic stress associated with elevate

173 Two have putative targeting signals: a myristoylation motif and a nuclear localization sequence

174 Expression of this fragment, which lacks the myristoylation motif and unique domain, was sufficient t

175 A heterologous N-myristoylation motif complemented N-terminal deletion mu

176 The CIL-7 myristoylation motif is essential for CIL-7 function and

177 ed, the protein does not have the N-terminal myristoylation motif nor is it subject to proteolytic ma

178 membrane association by mutating a putative myristoylation motif of AvrPto abolished the avirulence

179 The N-myristoylation motif of Pto complemented the cognate mot

180 An N-terminal myristoylation motif present in AKAP12alpha is shown to

181 Src protein-tyrosine kinase contains a myristoylation motif, a unique region, an Src homology (

182 The unique N-terminal end harbors a myristoylation motif, and we have shown here that PRMT8

183 uced HMGCLL1 sequence contains an N-terminal myristoylation motif; the putative modification site was

184 Expression of the Pto N-myristoylation mutant, pto(G2A), conferred recognition o

185 deficient in palmitoylation (palm(-)) and/or myristoylation (myr(-)) into bovine aortic endothelial c

186 N-terminal fatty acylations (N-myristoylation [MYR] and S-palmitoylation [PAL]) are cru

187 Analysis of Ncs1p mutants lacking myristoylation (Ncs1p(G2A)) or deficient in Ca2+ binding

188 We found that neither the N-terminal myristoylation nor the cysteine-rich RING H2 domain of r

189 The most common type of myristoylation occurs at an N-terminal glycine.

190 Protein N-myristoylation occurs by a covalent attachment of a C14:

191 Myristoylation occurs mainly at hSlo1 intracellular loop

192 In this case, myristoylation occurs within a portion of the preprotein

193 5, alpha7, beta3, and beta4); (2) N-terminal myristoylation of a 19S subunit (Rpt2); and (3) phosphor

194 establish a role for the post-translational myristoylation of a caspase-3-cleaved fragment of HTT, h

195 e predicted acylated residues confirmed that myristoylation of a glycine residue in the 2nd position

196 tment of AMPK to the mitochondria requires N-myristoylation of AMPKbeta by the type-I N-myristoyltran

197 We investigated the subcellular location and myristoylation of AtCPK5, a member of the Arabidopsis CD

198 Myristoylation of BBLF1 both facilitates its membrane an

199 al PTMs: postproteolytic N-acetylation and N-myristoylation of filensin.

200 N-myristoylation of Gag was necessary for association with

201 Myristoylation of GCAP1 does not significantly alter its

202 Myristoylation of HMGCLL1 affects its cellular localizat

203 Similarly, both myristoylation of MARCKS and cleavage of MARCKS by calpa

204 The physiologic role of myristoylation of MsrA remains to be elucidated.

205 is myristoylated at its N terminus, and that myristoylation of Neurl1 targets it to the plasma membra

206 Here, we present the role of myristoylation of NSC-1 in governing Ca(2+) binding and

207 difference between PKG isoforms results from myristoylation of PKGII.

208 The myristoylation of PRMT8 results in its association with

209 ticular use in providing evidence for native myristoylation of proteins of interest, proof of activit

210 N-Terminal myristoylation of proteins typically occurs cotranslatio

211 Myristoylation of PrP affected amyloid formation in two

212 he development of inhibitors that target the myristoylation of specific viral substrates more selecti

213 ases (NMT) catalyze co- or posttranslational myristoylation of Src family kinases and other oncogenic

214 genic potential in vivo We further show that myristoylation of Src kinase is essential to facilitate

215 While myristoylation of the amino terminus has long been known

216 arasite or its host's bloodstream to sustain myristoylation of the enormous quantity of variant surfa

217 Loss of expression or a defect of myristoylation of the Fus1 protein was observed in human

218 es alone or in combination does not abrogate myristoylation of the protein, suggesting utilization of

219 Myristoylation of the regulatory subunit of calcineurin

220 To test the hypothesis that myristoylation of the self-interacting GRASP domain rest

221 (post-translational myristoylation) and that myristoylation of this fragment is altered in a physiolo

222 observations provide clear evidence that the myristoylation of TRAM targets it to the plasma membrane

223 These findings indicate N-terminal myristoylation of Z plays a key role in arenavirus buddi

224 been presented on the relative importance of myristoylation, of ionic interactions between protein an

225 molecular mechanism for the modest effect of myristoylation on binding, wherein the membrane provides

226 Here, we investigated the effects of N-myristoylation on the structure, dynamics, and conformat

227 ybasic mutants by introduction of a site for myristoylation or by coexpression of betagamma failed to

228 Unlike prenylation and myristoylation, palmitoylation is a reversible covalent

229 ization at the plasma membrane via potential myristoylation/palmitoylation anchors.

230 e context of Src-S3C/S6C interferes with its myristoylation/palmitoylation.

231 suggests there may be an alternative type of myristoylation pathway in mammalian cells.

232 tests indicated that ACS-4-dependent protein myristoylation perceives and translates the FA level int

233 ARL2 was found to lack covalent N-myristoylation, present on all other members of the ARF

234 Based on our results, we predict that myristoylation promotes binding of ChChd3 to the outer m

235 However, myristoylation rarely occurs at an internal amino acid r

236 A second, less common myristoylation reaction occurs internally at dibasic ami

237 most likely a transient intermediate of the myristoylation reaction of Nef and is modulated by agent

238 22), the predicted protein has an N-terminal myristoylation recognition sequence, and we show here th

239 N-myristoylation refers to the attachment of myristic acid

240 Mutation at putative myristoylation residue glycine 2 altered plasma membrane

241 Thus, myristoylation restricts the membrane orientation of the

242 These modifications include N-myristoylation, S-acylation (palmitoylation), prenylatio

243 DeltaN to the PDK1 PH domain or the FRS2beta myristoylation sequence also induced Mek1 activation.

244 zation was reproduced using the heterologous myristoylation sequence from v-src.

245 pically driven to the plasma membrane by the myristoylation sequence of c-Src, and by mutation of a p

246 of spine fusion, an effect that required the myristoylation sequence.

247 chor to membranes through a 5' MGXXXS/T(R) N-myristoylation sequence.

248 This post-proteolytic N-myristoylation serves as an activating switch, enhancing

249 Furthermore, the N-myristoylation signal is necessary but not sufficient to

250 C2 domain is functionally equivalent to the myristoylation signal of c-Src, suggesting that it is an

251 Although the Gag-derived myristoylation signal targets the v-Abl protein to the p

252 d two other proteins that bear an N-terminal myristoylation signal, human immunodeficiency virus type

253 Src insert but depends on the amino-terminal myristoylation signal.

254 rmation when targeted to the membrane with a myristoylation signal.

255 we artificially membrane-targeted PTEN by a myristoylation signal.

256 of basic residues in the MA domain and an N-myristoylation signal.

257 have a similar structure, but they lack the myristoylation signal.

258 kinase, Src homology 2 or 3 domains, or the myristoylation site all inhibit conjugation to backgroun

259 d their only GRASP65 relative (Grh1) lacks a myristoylation site and has even been suggested to act i

260 t, despite extensive sequence variation, the myristoylation site and SH2 binding motifs were complete

261 89 to 91 amino acids with an amino-terminal myristoylation site and six SH2 binding motifs, showing

262 Analysis of the golli protein identified a myristoylation site at the C terminus of the golli domai

263 MK44 truncated at a putative N-myristoylation site did not produce current when express

264 Mutation of the myristoylation site Gly-2 abrogated membrane location.

265 ristoylation because mutation of a predicted myristoylation site in TRAM (TRAM-G2A) brought about dis

266 Mutation of the putative myristoylation site of SLAP-2 compromised its inhibitory

267 Mutation of the myristoylation site on golli disrupted its association w

268 it did not evoke inhibition of NHE3 unless a myristoylation site was added.

269 A predicted N-terminal myristoylation site was functionally analyzed.

270 ly, we show that the mutation of a predicted myristoylation site within the NH(2)-terminal of SLAP-2

271 ow that the N-terminal domain, including its myristoylation site, and the second EF-hand, which is in

272 ow that the N-terminal domain, including its myristoylation site, the central alpha-helix, and the C-

273 hat contains two automethylation sites and a myristoylation site.

274 d ligand-binding domains and three potential myristoylation sites located near the N terminus.

275 ch contains minimally the palmitoylation and myristoylation sites on Lyn, was compared with another i

276 odds with (i) its lack of palmitoylation or myristoylation sites that tailor its isoforms AKAP18alph

277 gh consensus CK2, PKC phosphorylation, and N-myristoylation sites, and may represent an essential ste

278 ion, one tyrosine phosphorylation, and one N-myristoylation sites.

279 , phosphokinase (PK)C phosphorylation, and N-myristoylation sites.

280 is abolished by a mutation that blocks its N-myristoylation, Snf4p is shifted to the nucleus.

281 es are modified by palmitoylation as well as myristoylation, Src itself is only myristoylated.

282 , from 100% of control (AcylTyA-GFP) for the myristoylation tag and PIP(2)-binding domain, to one-thi

283 icle budding and into EMVs, including: (i) a myristoylation tag; (ii) a phosphatidylinositol-(4,5)-bi

284 ipid modifications such as palmitoylation or myristoylation target intracellular proteins to cell mem

285 vo and identify cis-targeting motifs such as myristoylation that are necessary for EV-cargo associati

286 Myristoylation, the covalent linkage of a hydrophobic C1

287 In the absence of myristoylation, the R state predominates, and consequent

288 co-/post-translational modification known as myristoylation, the transfer of myristic acid (a 14-carb

289 N-myristoylation was also indispensable for early and dist

290 d lpxM single and double mutants showed that myristoylation was altered at lower temperature.

291 AtCPK5 membrane association, indicating that myristoylation was essential for membrane binding.

292 Loss of the site of N-myristoylation was found to affect neither AKAP macrosco

293 ing was essential for both phenotypes, while myristoylation was less critical.

294 was myristoylated in a cell-free extract and myristoylation was prevented by converting the glycine a

295 bstrate for plant N-myristoyltransferase and myristoylation was prevented by converting the glycine a

296 Myristoylation was studied as a means to introduce pepti

297 ur results suggest that targeting Src kinase myristoylation, which is required for Src kinase associa

298 Among these regulatory mechanisms is N-myristoylation, whose biological role has been elusive.

299 ng G i proteins to the membrane, the role of myristoylation with regard to interaction with activated

300 residue at position 6 to promote N-terminal myristoylation, yielding the construct GCAP1 E6S/P50L.