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1 lf-assembled native protein-lipid complexes (Nanodiscs).
2 he use of nanolipoprotein particles (NLPs or nanodiscs).
3 g interface of the P450-cytb5 complex in the nanodisc.
4 ane-bound protein YopB inserted into a lipid nanodisc.
5 narrow dispersity of lipid molecules in the nanodisc.
6 is via functional reconstitution of Aer into nanodiscs.
7 ture of the mouse TRPML1 channel embedded in nanodiscs.
8 r homodimers inserted into lipid bilayers of Nanodiscs.
9 n vitro self-assembling phospholipid bilayer nanodiscs.
10 d streamline analysis of heterogeneous-lipid Nanodiscs.
11 biotin transporter into phospholipid bilayer nanodiscs.
12 y in both protein assemblies and lipoprotein Nanodiscs.
13 al stability of the protein is higher in the nanodiscs.
14 platelets and assembled it into phospholipid nanodiscs.
15 lows the study of transmembrane signaling in nanodiscs.
16 example detailed herein, are included in the nanodiscs.
17 oluble nanoscale lipid bilayers, also termed nanodiscs.
18 ate the colocalization of the two enzymes in nanodiscs.
19 nally active membrane proteins inserted into nanodiscs.
20 ompared with nanorods and lower aspect-ratio nanodiscs.
21 PC1 that was incorporated into lipid bilayer nanodiscs.
22 ergy coupling of OpuA reconstituted in lipid nanodiscs.
23 gent-solubilized state or reconstituted into nanodiscs.
24 ve analyzed the translocation reaction using nanodiscs.
25 PG-nanodiscs, respectively, compared to POPC-nanodiscs.
26 protein complexes with GSL incorporated into nanodiscs.
27 o the elliptical shape recently reported for nanodiscs.
28 was monomeric in POPC-, POPC/POPG-, and POPG-nanodiscs.
29 ntermediate states for P-gp in lipid bilayer nanodiscs.
30 scherichia coli FF embedded in lipid bilayer nanodiscs.
31 with molecular dynamics studies of MSP1D1 in Nanodiscs.
32 were formed in the presence of bicelles and nanodiscs.
33 yloid polypeptide intermediate stabilized in nanodiscs.
34 nts after its cotranslational insertion into nanodiscs.
35 tificial lipid bilayers such as liposomes or nanodiscs.
36 and reconstitution of membrane proteins into nanodiscs.
37 constant amount of charge, for POPS and POPC Nanodiscs; 4) in contrast to some previous work, POPC on
38 e preparation of a range of stepwise-smaller nanodiscs (6- to 8-nm diameter) to overcome this limitat
40 -isolated lipids in purified KcsA-containing nanodiscs allows determination of preferential lipid-pro
43 onstrate that a combination of lipid bilayer nanodiscs and a multiplexed silicon photonic analysis te
44 port cycle, we have reconstituted MalFGK2 in nanodiscs and analyzed its conformations under 10 differ
48 constitution of the maltose transporter into nanodiscs and demonstrate that this system is ideally su
49 e combination of immobilizing the protein in Nanodiscs and footprinting with FPOP is a feasible appro
51 he transmembrane domains of mouse TMEM16A in nanodiscs and in lauryl maltose neopentyl glycol as dete
53 analyses of synaptobrevin reconstituted into nanodiscs and into liposomes now show that most of its S
54 kDa transmembrane protein, was inserted into nanodiscs and labeled with deuterium oxide under native
55 nds and small diffusing particles, including nanodiscs and liposomes containing membrane protein rece
56 ed on 6 x 6 periodic arrays of both recessed nanodiscs and nanorings to elucidate the differences in
57 y, unlike nanospheres, larger-sized hydrogel nanodiscs and nanorods are internalized more efficiently
58 constructing membrane-like MCP2901-inserted Nanodiscs and phosphorelay activity assays, we demonstra
59 membrane protein loaded nanodiscs from empty nanodiscs and protein aggregates results in monodisperse
60 e complexity of the spectra in heterogeneous Nanodiscs and that the lipid composition can be determin
61 l-2-oleoyl-sn-glycero-3-phosphocholine lipid nanodiscs and the kinetics of activation at 15 degrees C
63 on of functional ion pumps incorporated into nanodiscs and their subsequent analysis by several bioph
64 des effective electron imaging of liposomes, nanodiscs and viruses as well as comprehensive visualiza
65 ed individually in nanoscale lipid patches, "nanodiscs", and directly immobilized on unmodified gold
66 nanoscale phospholipid particles (so-called nanodiscs) and measured their ability to bind arrestin.
67 nanometer scale discoidal membrane bilayers (nanodiscs), and potentials were measured using spectropo
68 sium channels, reconstituted them into lipid nanodiscs, and performed single-molecule FRET confocal m
69 rking with large membrane protein systems in nanodiscs, and provide guidelines on the setup of NMR no
70 led with LRET probes, reconstituted in lipid nanodiscs, and the distance between the NBDs was measure
72 the mechanism of formation of polymer-based nanodiscs are characterized by light scattering, NMR, FT
87 The substrates consist of sub-1-microm gold nanodisc arrays which display dimension-tunable plasmon
90 , we not only demonstrated the usefulness of nanodiscs as a membrane-mimicking system, but also showe
92 de a foundation for future studies utilizing nanodiscs as a platform for launching membrane proteins
93 highlight the potential of the use of native nanodiscs as a tool in the study of ion channels, and of
95 resent work, we explore phospholipid bilayer nanodiscs as membrane mimics and employ electron microsc
96 probed the activities of these enzymes with nanodiscs as membrane mimics to determine whether they c
97 an does oligomeric rhodopsin in liposomes or nanodiscs, as assessed by stabilization of metarhodopsin
100 Depending on the target protein of interest, nanodisc assembly and purification can be achieved withi
101 of full-length human TRPM4 embedded in lipid nanodiscs at 3-angstrom resolution, as determined by si
102 sented here are charge measurements on lipid Nanodiscs at 20 degrees C in 100 mM NaCl, 50 mM Tris, at
103 ding cassette exporter MsbA reconstituted in nanodiscs at 37 degrees C while it performs ATP hydrolys
105 ly important property for the development of nanodisc-based characterization methodologies or biotech
109 phosphorylated light-activated Rh (P-Rh*) in nanodiscs binds arrestin-1 essentially as well as P-Rh*
110 V-ATPase conformations with the structure of nanodisc-bound Vo revealed that Vo is halted in rotation
111 st aspects of the insertion of proteins into nanodiscs by combining cell-free expression, noncovalent
112 dodecyl maltoside (DDM) and in phospholipid nanodiscs by monitoring the spatial positions of transme
113 bacteriorhodopsin, we show that our smaller nanodiscs can also be beneficial for the structural char
114 us of this protocol is on protein NMR, these nanodiscs can also be used for (cryo-) electron microsco
115 show here that small lipid bilayers known as nanodiscs can be used to chaperone the in vitro expressi
121 oupled receptors in cotranslationally formed nanodisc complexes demonstrate the versatility of this a
122 hat is a hybrid between a NPL and an organic nanodisc composed of phospholipids and lipoproteins.
123 ontours of the light-adapted monomeric bR in nanodiscs composed of different lipid ratios exhibited h
124 tocycle intermediates of bR reconstituted in nanodiscs composed of different ratios of the zwitterion
125 h styrene:maleic acid moieties that can form nanodiscs containing a planar lipid bilayer which are us
126 n addition, charge measurements were made on Nanodiscs containing an Escherichia coli lipid extract.
128 spectra and lipid stoichiometries of intact Nanodiscs containing lipid-raft associated sphingomyelin
129 n of the P450-cytb5 complex in peptide-based nanodiscs, containing no detergents, has been demonstrat
130 rate that high-density lipoprotein-mimicking nanodiscs coupled with antigen (Ag) peptides and adjuvan
131 full-length, purified and reconstituted into nanodiscs, couples to G proteins upon direct activation
132 served between Akt(PHD) and PI(3,4,5)P3-free nanodiscs, demonstrating that PI(3,4,5)P3 is required fo
133 dies whereas the magnetic-alignment of macro-nanodiscs (diameter of > ca. 40 nm) can be exploited for
138 allel screening of protein interactions with nanodisc-embedded lipids, glycolipids, and membrane prot
140 s upon addition of the native ligand NADH to nanodisc-embedded VDAC-1 resemble those of micelle-embed
141 Using negative stain electron microscopy and nanodisc-embedding to provide a membrane-like environmen
142 termination, due to their smaller size these nanodiscs enable the investigation of interactions betwe
143 icon photonic microring resonator arrays and nanodiscs enables rapid interrogation of biomolecular bi
144 teraction between small-molecule ligands and nanodisc-encapsulated membrane proteins, because the res
146 een peptide and small-molecule ligands and a nanodisc-encapsulated potassium ion channel protein, Kcs
147 ulating the timing of the kinetics, with the nanodisc environment leading to an earlier Schiff base d
148 t neutral at pH 7.4; 2) high-anionic-content Nanodiscs exhibit polyelectrolyte behavior; 3) 3 mM Ca(2
149 nodiscs (cNDs) which, compared with standard nanodiscs, exhibit enhanced stability, defined diameter
150 re-dependent structural changes, the polymer nanodiscs experience negligible structural evolution und
151 trates the utility of SSNMR experiments with Nanodiscs for examining lipid-protein interfaces and has
152 nts and pitfalls relating to optimization of nanodiscs for NMR spectroscopy and outline a strategy fo
154 es of this system and advantages provided by nanodiscs for structural and mechanistic studies of memb
155 e scaffold proteins (MSP) that do not affect nanodisc formation but shift the masses of nanodiscs in
156 n, has been incorporated in single copy into nanodiscs formed by a membrane scaffold protein encircli
158 rative separation of membrane protein loaded nanodiscs from empty nanodiscs and protein aggregates re
161 gly, we find that the monomeric rhodopsin in nanodiscs has a higher affinity for wild-type arrestin b
162 n this work, monomeric CYP3A4 solubilized in Nanodiscs has been studied for its ability to interact w
170 Here, we report the discovery of polymer nanodiscs, i.e., discoidal amphiphilic block copolymer m
171 t nanodisc formation but shift the masses of nanodiscs in a controllable way, eliminating isobaric in
174 otein complexes from amphipols, bicelles and nanodiscs in the gas phase for observation by mass spect
175 that membrane protein oligomers ejected from nanodiscs in the gas phase retain large numbers of lipid
176 nt micelles, bicelles, oriented bilayers, or nanodiscs, in order for them to be soluble or dispersed
179 y showed that the negative surface charge of nanodiscs increased as the content of DOPG or DMPG was i
181 ow that Akt(PHD) binds to both layers of the nanodisc, indicating proper incorporation of PI(3,4,5)P3
182 isualized in membranes, vesicle-inserted and nanodisc-inserted, allowing us to reconstruct two virtua
184 Although it is commonly agreed that the nanodisc is plain disk shaped, several more advanced str
185 We found that although only one SNARE per nanodisc is required for maximum rates of bilayer fusion
186 id-bilayer presentation of viral antigens in Nanodiscs is a new platform for evaluating neutralizing
187 ic strength-gated ATPase activity of OpuA in nanodiscs is at least an order of magnitude higher than
188 show that the conformation of KcsA in native nanodiscs is very similar to that in detergent micelles,
191 resonances of the heme chromophore in CYP3A4-Nanodiscs, LSPR spectroscopy is used to detect drug bind
193 limitations of the two-dimensional nature of nanodisc membranes that offers no compartmentalization.
194 sm measurements performed on CYP3A4 bound to Nanodisc membranes were used to characterize the orienta
195 de a proof-of-concept demonstrating that the nanodisc model membrane system represents a promising ex
196 NMR methods to characterize Rheb tethered to nanodiscs, monodisperse protein-encapsulated lipid bilay
197 odopsin-octylglucoside micelle and the empty nanodisc (MSP1D1-Nd) using both MS and tandem-MS modes o
200 We investigated this phenomenon in lipid nanodiscs (NDs) at equilibrium on a local scale, analyzi
201 with the CaR-ESI-MS assay implemented using nanodiscs (NDs) revealed that the PDs exhibited similar
202 y (ESI-MS) analysis combined with the use of nanodiscs (NDs) to solubilize glycolipids (GLs) has rece
203 d-release (CaR)-ESI-MS assay, which exploits nanodiscs (NDs) to solubilize glycolipids and mimic thei
204 aloganglioside ligand GM1, incorporated into nanodiscs (NDs), for cholera toxin B subunit homopentame
206 oscopy of membrane proteins in commonly used nanodiscs of 10-nm diameter were limited by the high mol
212 s of p75NTR, incorporated into lipid-protein nanodiscs of various sizes and compositions, by solution
213 t a protocol on the assembly of phospholipid nanodiscs of various sizes for structural studies of mem
214 fined lipid bilayer environment, lipoprotein Nanodiscs offer a promising cassette for membrane protei
216 ly detergent micelles but also lipid bilayer nanodiscs or bicelles can serve as a means for the gentl
217 rified EGF-bound EGFR dimers in phospholipid nanodiscs or vesicles, suggesting that the environment a
218 primary preparations of receptor-containing Nanodiscs, otherwise heterogeneous for number and orient
219 d protein aggregates results in monodisperse nanodisc preparations ideal for structural and functiona
220 onditions, using self-assembled phospholipid nanodiscs prepared with the zwitter-ionic lipid 1-palmit
221 NMR) studies on lyophilized, rehydrated POPC Nanodiscs prepared with uniformly (13)C-, (15)N-labeled
225 The cell-free synthesis in combination with nanodiscs provides a defined hydrophobic lipid environme
230 2 orders of magnitude in POPC/POPG- and POPG-nanodiscs, respectively, compared to POPC-nanodiscs.
232 detergent-free synthesis of membrane protein/nanodisc samples and the analysis by LILBID mass spectro
233 The solid-supported membrane assay with nanodisc samples provides reliable control over the ioni
235 emonstrate that a well ordered dense film of nanodiscs serves for non-destructive, label-free studies
237 f a domain 4-truncated PA pore inserted into nanodiscs showed that this domain does not significantly
240 we demonstrate that the combination of small nanodisc size, high deuteration levels of protein and li
241 hemoreceptor Tar from Escherichia coli using Nanodiscs, small ( approximately 10-nm) plugs of lipid b
244 coupling measurements confirmed that in the nanodiscs, SRII photoactivation induces helix movement i
246 uting unlabeled LeuT in phospholipid bilayer nanodiscs, subjecting them to hydrogen-deuterium exchang
250 lar to the particles formed in the so-called nanodisc system, which is based on N-terminal truncated
253 of fluorescence correlation spectroscopy and nanodisc technologies provides a powerful approach to ac
254 0 in the human heart, using a combination of Nanodisc technology and a nanohole plasmonic sensor call
256 ombining electron cryo-microscopy with lipid nanodisc technology to ascertain the structure of the ra
257 A pore and documented the value of combining nanodisc technology with electron microscopy to examine
259 omprehensive list of various applications of nanodisc technology with systematic analysis of the most
260 combination of cell-free synthetic biology, nanodisc-technology and non-covalent mass spectrometry p
263 o change the membrane composition of the CPR-nanodisc, the redox potential of both flavins became mor
265 , binding of a PI(3,4,5)P3-embedded membrane nanodisc to Akt(PHD) with a 10(3)-fold tighter affinity
266 oli rendered water soluble by insertion into nanodiscs to (i) measure saturable binding of CheA and C
267 incorporated SRII-HtrII dimeric complexes in nanodiscs to allow unrestricted probe access to the cyto
269 ering pores connecting v-SNARE-reconstituted nanodiscs to cells ectopically expressing cognate, "flip
272 the native-like environment of phospholipid nanodiscs undergo spontaneous transitions between two di
275 icant amounts of lipid are released from the nanodiscs upon insertion of larger protein complexes.
276 onstration of a protein-protein complex in a nanodisc using NMR structural studies and should be usef
277 ured the affinity of arrestin-1 for P-Rh* in nanodiscs using a fluorescence-based assay and found tha
278 ctionally native receptor structure, we made Nanodiscs using natural and synthetic lipids, assaying e
279 ning of isotope-labeled membrane proteins in nanodiscs using nuclear Overhauser enhancement spectrosc
280 e discoidal proteolipid particles or "native nanodiscs." Using circular dichroism and tryptophan fluo
283 Gq protein coupling to NTS1 in various lipid nanodiscs was significantly different, and the apparent
284 gle FoF1 molecules embedded in lipid bilayer nanodiscs, we now report the observation of Fo-dependent
285 asurements of F0F1 embedded in lipid bilayer nanodiscs, we observed that the ability of the F0 motor
286 hydrolysis, the NBDs of Pgp reconstituted in nanodiscs were never far apart during the hydrolysis cyc
287 ful preparation of novel peptide-based lipid nanodiscs, which are detergent-free and possesses size f
288 ical oxidation of proteins (FPOP), and lipid Nanodiscs, which are more similar to the native membrane
289 we provide evidence that reconstitution into nanodiscs, which are soluble disk-shaped phospholipid bi
290 o planar lipid bilayers directly from native nanodiscs, which enables functional characterization of
292 ng resonator arrays and phospholipid bilayer nanodiscs, which together allow multiplexed screening of
294 n alphaIIbbeta3 embedded in a lipid bilayer (nanodiscs) while bound to domains of the cytosolic regul
298 inally, we demonstrate the use of mixed belt nanodiscs with embedded membrane proteins to confirm the
300 A4 incorporated in surface-immobilized lipid Nanodiscs, with and without the effector alpha-naphthofl
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