ライフサイエンスコーパス: nanopore

1 ated, linearly charged protein through a sub-nanopore.

2 diates DNA and protein transport through the nanopore.

3 down single-molecule DNA transport through a nanopore.

4 monomers on the inner surface of solid-state nanopore.

5 cleotides through an enzyme is measured by a nanopore.

6 y move through the electric field inside the nanopore.

7 ing the DNA structures through a solid-state nanopore.

8 me and concentrated at the tip of a metallic nanopore.

9 the DNA sensing capabilities of a biological nanopore.

10 imination of the nucleotides in a biological nanopore.

11 nded DNA oligonucleotides trapped in an MspA nanopore.

12 aged surface potential difference across the nanopore.

13 olysin into a highly nucleic acids-sensitive nanopore.

14 ticles using a resistive pulse sensing (RPS) nanopore.

15 complex in a Mycobacterium smegmatis porin A nanopore.

16 by deliberate charge decorations inside the nanopore.

17 etween the NeutrAvidin molecule and the MspA nanopore.

18 e insights when designing charge patterns in nanopores.

19 hnology to characterize NPs by using protein nanopores.

20 aphy and redox species diffusion through the nanopores.

21 ersing the driving voltage across individual nanopores.

22 d septal junctions, and septal peptidoglycan nanopores.

23 cury droplets confined in organic-rich shale nanopores.

24 mical reaction driven by water quantities in nanopores.

25 d materials in nanofiltration and artificial nanopores.

26 recently emerged as powerful alternatives to nanopores.

27 lowing analyte solutions to pass through the nanopores.

28 eric walls and easy-to-engineer membrane DNA nanopores.

29 forced partitioning of polymers into protein nanopores.

30 k their microscopic movements in cylindrical nanopores.

31 onist, Nutlin-3, using low-noise solid-state nanopores.

32 the negatively charged surfaces of the glass nanopores.

33 t we can analyze their characteristics using nanopores.

34 dopamine (PDOPA) on asymmetric track-etched nanopores.

35 translocates through charge-mutated protein nanopores.

36 fferent mutations of alpha-hemolysin protein nanopores.

37 ation gradients applied over alpha-hemolysin nanopores.

38 the rational design of polymer-coated smart nanopores.

39 Four popular Nanopore aligners are supported and it is easily extensi

40 to change the sensing properties of alphaHL nanopores, allowing the detection and characterization o

41 for instance, by decreasing the size of the nanopores alone, it is possible to change their behavior

42 The wild-type protein nanopore alpha-hemolysin is used to capture individual D

43 MPSA)-modified-gold NPs using the biological nanopores alpha-hemolysin (alphaHL) and its M113N mutant

44 Solid-state nanopore analyses show it to be nucleosomal in size.

45 NA translocates through a 2.4 nm solid-state nanopore and reveals new interactions between short sing

46 We focus on two sensing principles: nanopores and amperometric microelectrode devices.

47 the non-uniform growth observed in parallel nanopores and cannot be explained by classic quasi-stead

48 tides, combined with polymerase tethering to nanopores and multiplexed nanopore sensors, should lead

49 DNA-based membrane nanopores and origami structures able to assemble into t

50 o ionic current rectification inside conical nanopores and other asymmetric nanostructures.

51 ranslocation dynamics of DNA through conical nanopores and provide a quantitative model for the trans

52 olecular sensing and transport studies using nanopores and separation of charged species.

53 UF hollow fiber membranes with well-defined nanopores and surface charges were developed via a singl

54 n can potentially become trapped in isolated nanopores and thus not recoverable.

55 osphotungstic acid are measured with protein nanopores and validated with NMR.

56 ing atomic force microscopy, break junction, nanopore, and covalently bridging gaps.

57 cessive DNA polymerase was conjugated to the nanopore, and the conjugates were complexed with primer/

58 e PE layer, the level of pH, the geometry of nanopore, and the thickness of the double layer.

59 he absolute limit of sensor miniaturization, nanopores are amenable to parallelization and could be u

60 hat the SECM images of 100 nm diameter Si3N4 nanopores are enlarged along the direction of the tip sc

61 encompassing the complete array with closer nanopore arrangement, whereas individual silica deposits

62 ally integrating microhotplatform (MHP) with nanopore array (NPA).

63 o perform nanopore SBS on an alpha-hemolysin nanopore array platform.

64 experimental evidence of diffusion zones at nanopore arrays and provide practical illustration that

65 Nanopore arrays with different ratios of pore center-to-

66 rlapped or independent diffusion profiles at nanopore arrays with rc/ra ratios of 21 +/- 2 and 91 +/-

67 Here, we report a rapid solid-state nanopore assay that is capable of resolving 5 hmC with h

68 mers convert to more closely packed membrane nanopore assemblies, which can subsequently recruit addi

69 ed DNA loading into waveguides equipped with nanopores at their floors is five orders of magnitude gr

70 The resulting raw nanopore-based de novo genome is structurally highly sim

71 In this study, a new nanopore-based detection scheme utilizing a borosilicate

72 a new tool that can improve the analysis of nanopore-based measurements.

73 that improves the accuracy and throughput of nanopore-based measurements.

74 Recently, we reported a single-molecule nanopore-based SBS strategy that accurately distinguishe

75 The nanopore-based sensing analysis has wide implications fo

76 ared with the traditional ELISA for PSA, the nanopore-based sensor assay is 50-100 fold more sensitiv

77 optofluidic chip, which consists of arrayed nanopore-based sensors fabricated from anodic aluminum o

78 Recently, a nanopore-based sequencing instrument, the Oxford Nanopor

79 Translocation of DNA is a crucial step in nanopore-based sequencing platforms, where control over

80 Nanopore-based sequencing techniques can reconstruct pro

81 Recently, we presented a nanopore-based sequencing-by-synthesis (Nanopore-SBS) ap

82 ched (PCTE) membranes with differently sized nanopores between PDMS slabs containing embedded microch

83 A stacked plasmonic nanowell-nanopore biosensor strongly suppresses the background fl

84 antify the contribution of the EOF through a nanopore by investigating the permeation of alpha-cyclod

85 e we show that the current carried through a nanopore by ions allows monitoring conformational change

86 ocation of a semi-flexible polymer through a nanopore by means of a modified version of the iso-flux

87 The blocking of the alpha-hemolysin nanopore by rhodamines could be utilized in DNA sequenci

88 e molecules can be achieved with solid-state nanopores by using digitally encoded DNA nanostructures.

89 ipets for SECM imaging of single solid-state nanopores by using nanopipet-supported interfaces betwee

90 proach, single RNA molecules captured by the nanopore can freely fold from the unstructured state wit

91 In situ studies show that nanopores can absorb and eliminate a large number of rad

92 ogether, our results reveal that solid-state nanopores can be a valuable platform for the ultrasensit

93 sensing volume of bilayer-coated solid-state nanopores can be used to determine the approximate shape

94 Here, we show that solid-state nanopores can be used to directly observe individual kno

95 rent measurements through electrolyte-filled nanopores can characterize single native proteins in an

96 Nanopores can thus be used to immobilize proteins on a s

97 ions, [Formula: see text], on nanopore size, nanopore chemistry, and nanopore morphology.

98 r individually addressable electrodes of the nanopore chip.

99 The different nanopore conductance between DNA and RNA translocation s

100 ing phosphatidylserine (PS) externalization, nanopore-conducted currents, membrane blebbing, and cell

101 methods such as mechanical break junctions, nanopores, conductive atomic force microscopy, scanning

102 Recent studies only focus on the effect of nanopore confinement on single-well performance with sim

103 kable physical characteristics of asymmetric nanopores constitutes a new framework to design multifun

104 The designed nanopore construct successfully detected the capture of

105 Precisely size- and geometry-tuned nanopores could find application in molecular sensing, D

106 clable LiBr was utilized as the template for nanopores creation while the polymer was processed at th

107 NA knots are observed as short spikes in the nanopore current traces of the traversing DNA molecules

108 vo genome sequencing and assembly using only nanopore data remain challenging.

109 Using Pacific Biosciences and Oxford Nanopore data, Circlator correctly circularized 26 of 27

110 ed Illumina, Pacific Biosciences, and Oxford Nanopore data; and the scalable clustering of hundreds o

111 ic surveillance system that utilizes a novel nanopore DNA sequencing instrument.

112 Nanopore DNA strand sequencing has emerged as a competit

113 a significant fraction of their porosity as nanopores, dominate the reactive surface area of diverse

114 ogies like those offered by PacBio or Oxford Nanopore), efficient k -mer processing is still crucial

115 is current-potential (i-V) curves in conical nanopore electrokinetic measurements, is quantitatively

116 This method provides insight into the nanopore engineering for biosensing, making aerolysin ap

117 Since a large amount of nanopores exist in tight oil reservoirs, fluid transport

118 ning a new class of nanoscale sensors dubbed nanopore extended field-effect transistor (nexFET) that

119 However, only a few groups explored this nanopore for nucleic acids detection.

120 Here, we explore the potential of sub-nanopores for single-molecule protein identification (SM

121 n the controlled fabrication of subnanometre nanopores for use in nanofluidics.

122 nsist of single bilayers, as demonstrated by nanopore formation experiments and confocal fluorescence

123 Purpose To assess for nanopore formation in bone marrow cells after irreversib

124 Conclusion IRE can induce nanopore formation in bone marrow cells.

125 as: drug delivery, biosensing, and synthetic nanopore formation.

126 after adsorption of biomolecules inside the nanopores from a reference reflection spectrum recorded

127 ethod to modify the inner surface of polymer nanopores fully compatible with the fabrication of nanof

128 ven DNA translocation can be affected by the nanopore geometry and hence the available configurationa

129 stic single-molecule detection using protein nanopores has found widespread application in bioanalyti

130 ts revealed that proteins trapped inside the nanopore have bulk-like properties.

131 pores is modeled by adopting a bullet-shaped nanopore having a pH-tunable zwitterionic surface, focus

132 Membrane nanopores-hollow nanoscale barrels that puncture biologi

133 elucidate the non-trivial interplay between nanopore hydrophilicity and surface barriers on the over

134 with four different polymers captured in the nanopore in such ternary complexes were clearly distingu

135 ts Scanning electron microscopy demonstrated nanopores in bone marrow cells only after IRE (P , .01).

136 It is used to create functional nanopores in DIBs composed of phosphocholine using the p

137 We demonstrate the fabrication of individual nanopores in hexagonal boron nitride (h-BN) with atomica

138 rthworm E. fetida, inserts large conductance nanopores in lipid membranes containing sphingomyelin.

139 fundamentally different approach to produce nanopores in sheet substrates under dry, ambient conditi

140 unique strategy for direct synthesis of gold nanopores in solution without the need for sacrificial t

141 Methods for generating nanopores in substrates typically involve one or more we

142 Single-polymerase coupling to a nanopore, in combination with the Nanopore-SBS approach,

143 Pacific Biosciences and Oxford Nanopore increase throughput also but require high input

144 is deposited on the orifice of a solid-state nanopore inside a focused-ion beam (FIB) system.

145 across the nucleic envelope via insertion of nanopores into the bilayers.

146 Central to the method is the introduction of nanopores into the organic semiconductor thin films via

147 e show that with its specific geometry, such nanopore is capable of exhibiting several interesting be

148 in tight oil reservoirs, fluid transport in nanopores is complex due to large capillary pressure.

149 racy of sequencing single DNA molecules with nanopores is continually improving, but de novo genome s

150 urrent rectification behavior of bioinspired nanopores is modeled by adopting a bullet-shaped nanopor

151 face chemical characteristics of solid-state nanopores is of great interest as it provides the means

152 tructure, ultrathin nanosheets with abundant nanopores, large surface area, and highly dispersed ultr

153 We demonstrate that nanopore long reads are superior to short reads with reg

154 enomena in ionic transport, and suggest that nanopores may also further our understanding of transpor

155 In combination with NMR data, the nanopore measurements showed that the addition of Nutlin

156 h the aperture of an antibody modified glass nanopore membrane (AMGNM) with the application of a mech

157 meable ultrafiltration membrane, the silicon nanopore membrane (SNM), designed with approximately 7 n

158 reduce sensor response time, open-ended PSi nanopore membranes were used in a flow-through sensing s

159 ogies such as Pacific Biosciences and Oxford Nanopore MinION are capable of producing long sequencing

160 Recently, genome assemblies using Oxford Nanopore MinION data have attracted much attention due t

161 q using the long-read single-molecule Oxford Nanopore MinION sequencer is able to identify and quanti

162 Here, we use the Oxford Nanopore MinION sequencer to identify 7,899 'full-length

163 The Oxford Nanopore MinION sequencer, currently in pre-release test

164 The recently released Oxford Nanopore MinION sequencing platform presents many innova

165 ies from both Pacific Biosciences and Oxford Nanopore MinION show excellent continuity and completene

166 pore-based sequencing instrument, the Oxford Nanopore MinION, has become available, and we used this

167 mmarize key technical features of the Oxford Nanopore MinION, the dominant platform currently availab

168 Finally, we use metallized nanopores modified with homocysteine for the detection o

169 ], on nanopore size, nanopore chemistry, and nanopore morphology.

170 e ion current modulation through the protein nanopore MspA to observe translocation of helicase Hel30

171 and prospects of integrating 2D materials in nanopores, nanogaps and similar devices for single molec

172 Exceptionally high surface area and ordered nanopores of a metal-organic framework (MOF) are exploit

173 ble of exhibiting robust dynamics inside the nanopores of a MOF.

174 y integrating high density Ag NPs inside the nanopores of diatom biosilica, which is not achievable b

175 onstrate that hydronium ions confined in the nanopores of zeolite HBEA catalyse aqueous phase dehydra

176 of small uncharged molecules into and across nanopores, one often uses ion currents.

177 pecies and the local electric field near the nanopore openings play a key role, yielding profound and

178 ing in the dynamics of ion transport through nanopores or nanochannels is important for sensing, nucl

179 s, physicochemical effects of confinement in nanopores, pi interactions of aromatic compounds with po

180 Here, a brief recent history of the nanopore platform is provided, key papers and innovation

181 cation behaviors are observed using the gold nanopore, potentially enabling new capabilities in biose

182 Here, we show that single graphene nanopores preferentially permit the passage of K(+) cati

183 across a wild-type alpha-hemolysin (alphaHL) nanopore provides structural information about different

184 ze ion diffusion profiles at the orifices of nanopores (radius (ra) of 86 +/- 6 nm) in array format:

185 ybrid methods can be used to assemble Oxford Nanopore reads into informative multi-chromosome assembl

186 data or detection of overlaps between Oxford Nanopore reads to improve accuracy.

187 h the unzipping of a miRNA-DNA duplex in the nanopore recorded as a resistive current pulse.

188 The free energy of CO3 (2-) hydrolysis in nanopores reduces with a decrease of water availability.

189 1 M KCl, while the duplex dwell time in the nanopore remained acceptable for pulse detection and cou

190 If integrated into a nanopore, RT would provide a unique approach to sequenci

191 Oxford Nanopore's MinION device has matured rapidly and is now

192 th oligonucleotide-based polymers to perform nanopore SBS on an alpha-hemolysin nanopore array platfo

193 pling to a nanopore, in combination with the Nanopore-SBS approach, can provide the foundation for a

194 ed a nanopore-based sequencing-by-synthesis (Nanopore-SBS) approach, which used a set of nucleotides

195 The combination of nanopore sensing and nucleic acid aptamer recognition co

196 fferences between biological and solid-state nanopore sensing and provides strategies for subnanomola

197 erature studies coupled with resistive-pulse nanopore sensing enable the quantification of a variety

198 Nanopore sensing is an attractive method in this regard

199 Nanopore sensing is an emerging technology for the singl

200 Previous demonstrations of nanopore sensing with temperature control have utilized

201 In nanopore sensing, changes in ionic current are used to a

202 ingle-molecule dielectrophoretic trapping to nanopore sensing.

203 Ren et al. combine a nanopore sensor and a field-effect transistor, whereby g

204 Here, we exploit a protein nanopore sensor for Pim kinases that bears a pseudosubst

205 Here we investigate solid-state nanopore sensors with an embedded gold film, fabricated

206 erase tethering to nanopores and multiplexed nanopore sensors, should lead to new high-throughput seq

207 ceptionally promising route is in the use of nanopore sensors.

208 th congenital abnormalities using the MinION nanopore sequencer and a novel computational pipeline-Na

209 loration of patient genome sequencing with a nanopore sequencer and demonstrate the value of long-rea

210 The first nanopore sequencer available, the MinION from Oxford Nan

211 ford Nanopore Technologies MinION using this nanopore sequencer's ionic current signal.

212 and accuracy, coupled with higher throughput nanopore sequencers, mean that human genome sequencing a

213 ed data storage system that uses error-prone nanopore sequencers, while still producing error-free re

214 latform may be implemented in practice using nanopore sequencers.

215 Recently, portable, real-time, nanopore sequencing (RTnS) has become available.

216 These results demonstrate that nanopore sequencing can be used to deconvolute individua

217 Nanopore sequencing data from a single 18-h run was used

218 e describe the generation of a comprehensive nanopore sequencing data set with a median read length o

219 Pore, a toolkit for exploring and analysing nanopore sequencing data.

220 In nanopore sequencing devices, electrolytic current signal

221 Nanopore sequencing has been available to researchers fo

222 thms in order to fully utilize the extent of nanopore sequencing potential.

223 Nanopore sequencing promises long read-lengths and singl

224 PoreSeq increases nanopore sequencing read accuracy of M13 bacteriophage D

225 Map, a mapping algorithm designed to analyse nanopore sequencing reads, which progressively refines c

226 Realizing the democratic promise of nanopore sequencing requires the development of new bioi

227 nces in single molecule real-time (SMRT) and nanopore sequencing technologies have enabled high-quali

228 Recent advances in nanopore sequencing technology have led to a substantial

229 Prior to this, nanopore sequencing technology was mainly used to analyz

230 Here, we have used whole-genome Nanopore sequencing to characterize several CGRs that or

231 Canu introduces support for nanopore sequencing, halves depth-of-coverage requiremen

232 ad DNA sequencing technologies, specifically Nanopore sequencing, have made possible the rapid identi

233 the samples (n = 19) by Sanger and by Oxford Nanopore sequencing.

234 The nanopores serve to enhance the dopant/organic semiconduc

235 The influences of the nanopore shape, solution pH, and bulk salt concentration

236 en transported through ultrathin solid-state nanopores, short DNA fragments containing thymine modifi

237 Meanwhile, nanopores shrink (self-heal) during radiation, and their

238 By identifying the nanopore signatures and measuring their time-dependent p

239 In this study, using a recently validated nanopore silica film based method, we measured serum hep

240 stor (nexFET) that combine the advantages of nanopore single-molecule sensing, field-effect transisto

241 izes were divided into five regions based on nanopore size distribution.

242 librium conversions, [Formula: see text], on nanopore size, nanopore chemistry, and nanopore morpholo

243 on of the protein to the chemically modified nanopores slows down the translocation process to tens o

244 tion, leading to a loss of gel and capillary nanopores, smoother pore surfaces, and reduced porosity.

245 Here, we report a nanopore snapshot approach combined with coarse-grained

246 ical communication) were imparted by protein nanopores spanning the lipid bilayer formed at the inter

247 gy, and consider what the next-generation of nanopore structures could be and where further practical

248 In particular, the preparation of through nanopore structures is extremely challenging.

249 ical Note, we describe a method to fabricate nanopore-supported Pt nanoparticle electrodes and their

250 on-specific adsorption between alpha-Syn and nanopore surface to ensure successful and continuous det

251 has a local maximum as the curvature of the nanopore surface varies, and if it is lower than the iso

252 d: ADEPT, which uses a physical model of the nanopore system to characterize short-lived events that

253 from Pacific Bioscience (PacBio) and Oxford Nanopore Technologies (ONT) offer the opportunity to pha

254 Time (SMRT) sequencing technology and Oxford Nanopore technologies (ONT) produce reads over 10 kb in

255 ither Pacific Biosciences (PacBio) or Oxford Nanopore technologies and achieves a contig NG50 of >21

256 ncing instrument that was released by Oxford Nanopore Technologies in 2014, producing long sequencing

257 ata expected to result from extensive use of nanopore technologies in the future.

258 The use of nanopore technologies is expected to spread in the futur

259 The Oxford Nanopore Technologies MinION is a portable device that u

260 The Oxford Nanopore Technologies MinION sequencer enables the selec

261 d the strength of this effect for the Oxford Nanopore Technologies MinION sequencer.

262 eparation method for sequencing on an Oxford Nanopore Technologies MinION sequencer.

263 cal microbiology laboratory using the Oxford Nanopore Technologies MinION sequencer.

264 ne and adenosine methylation with the Oxford Nanopore Technologies MinION using this nanopore sequenc

265 red from peptides could find applications in nanopore technologies such as single-molecule sensing an

266 ds for the portable MinION sequencer (Oxford Nanopore Technologies) and the Illumina range of instrum

267 sequencer available, the MinION from Oxford Nanopore Technologies, is a USB-connected, portable devi

268 Updates in nanopore technology have made it possible to obtain giga

269 In contrast, nanopore technology is exquisitely sensitive to single i

270 logies MinION is a portable device that uses nanopore technology that can directly sequence DNA molec

271 discuss the future challenges of developing nanopore technology, and consider what the next-generati

272 yer on an IR-transparent layer with embedded nanopores, the nanoporous metallized polyethylene textil

273 Because of the high aspect ratio of PSi nanopores, the performance of closed-ended PSi sensors i

274 fusion, and contained an increased number of nanopores, the septal peptidoglycan perforations that li

275 create individual size-quantized triangular nanopores through an h-BN sheet.

276 Here we use solid-state nanopores to study the time-dependent kinetics of alpha-

277 Using a nanopore, transitions in individual Phi29 DNAP complexes

278 Here, we report the results of nanopore translocation experiments and molecular dynamic

279 account for the differences observed in our nanopore translocation experiments.

280 veloped single-molecule picometer-resolution nanopore tweezers (SPRNT), a single-molecule technique i

281 trokinetic transport through broadly defined nanopore-type devices.

282 taking into account the capture volume of a nanopore, typically 10(8)-10(10) times smaller than the

283 namic opening and closing of the ion channel nanopores using single-walled carbon nanotubes (SWNTs).

284 ocation of positively charged MDM2 through a nanopore was detected at the applied negative voltage, t

285 Previously, the M2MspA nanopore was shown to be sensitive enough to distinguish

286 e translocation time of the knot through the nanopore, we estimate that the majority of the DNA knots

287 geometrical asymmetry of a conically shaped nanopore, we examine how DNA dynamics depends on the dir

288 d in molecular dynamics simulations of model nanopores, where the principles underlying hydrophobic g

289 ective etching of graphene to form edges and nanopores, which have unique chemical and physical prope

290 eaming creates new surface area and enlarges nanopores, which helps relieve steric hindrance to adsor

291 is side, leading to unzipping outside of the nanopore with higher residual current and faster unzippi

292 nic current rectification (ICR) in a conical nanopore with its surface modified by pH-tunable polyele

293 is approach combines detection via a protein nanopore with modification of its interaction behavior u

294 Complete blockage of the nanopore with Pt metal forms a closed bipolar nanopartic

295 nopipet with a thin layer of carbon yields a nanopore with tunable surface charge and chemical state

296 Integration of protein nanopores with high-resolution scanning ion conductance

297 Moreover, nanopores with internal protein adaptors might find furt

298 ade selective for a given MMP by filling the nanopores with synthetic polymeric substrates containing

299 ion leads to periodic patterns of triangular nanopores with uniform size.

300 dual silica deposits were formed around each nanopore within the more widely spaced array.