1 d 14-membered ring macrolides pikromycin and narbomycin.

2 f the PikC(D50N) mutant co-crystallized with narbomycin (1.85A resolution) and YC-17 (3.2A resolution

3  (2), neomethymycin (3), pikromycin (4), and narbomycin (5) produced by Streptomyces venezuelae .

4 ng macrolactone 10-deoxymethynolide, whereas narbomycin and pikromycin are derived from the 14-member

5 antibiotics, including the natural ketolides narbomycin and pikromycin.

6 olide antibiotics methymycin, neomethymycin, narbomycin, and pikromycin in Streptomyces venezuelae, i

7 e for Glu-94 in YC-17 binding and Glu-85 for narbomycin binding.

8 ibiotics (such as erythromycin, tylosin, and narbomycin) depend ultimately on the glycosylation of ot

9 iple sugar moieties, whereas others (such as narbomycin) have only single sugar substituents.

10 ion of novel macrolide substrates similar to narbomycin, in particular, ketolides, a promising class

11 ne moiety of the native substrates YC-17 and narbomycin is bound in two distinct buried and surface-e

12 rns for the 12-(YC-17) and 14-membered ring (narbomycin) macrolides, respectively.

13  the final glycosylated products pikromycin, narbomycin, methymycin and neomethymycin) and desR (a re

14 ucer of tylosin) by importing genes from the narbomycin producer Streptomyces narbonensis.

15 enous substrates YC-17 (resolution 2.35 A)or narbomycin (resolution 1.7 A).

16 is responsible for the C-12 hydroxylation of narbomycin to picromycin.

17 0N), the desosamine moiety of both YC-17 and narbomycin was bound in a catalytically productive "buri

18 products, macrolide intermediates (YC-17 and narbomycin) were hydroxylated with high efficiency.

19  catalyze the in vitro C-12 hydroxylation of narbomycin with a kcat of 1.4 s-1, which is similar to t