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3 f de novo DAG and indicated similar rates of nascent acyl groups into the Kennedy pathway and acyl ed
5 in which local actin assembly forces buffer nascent adhesion sites from the mechanical effects of re
6 ntrast, defective farnesylation mislocalizes nascent adhesion sites, suggesting that LKB1 farnesylati
9 etween the front of the lamellipodium, where nascent adhesions are positioned in rows, and the base o
10 lyse rigidity sensing after associating with nascent adhesions under rigidity-dependent tension downs
13 sion lines, indicating a significant flux of nascent and elongated acyl-CoAs into the sn-3 position o
14 ors drove CD73 expression, which marked both nascent and full activation of a mesenchymal-like melano
15 se limitations by simultaneously quantifying nascent and mature mRNA in individual cells, and incorpo
16 urement of the abundance and localization of nascent and mature RNA transcripts in fixed, single cell
18 urons face unique challenges of transporting nascent autophagic vacuoles (AVs) from distal axons towa
22 marked by TbRP2 recruitment, is coupled with nascent basal body assembly, monitored by localization o
23 teraction of residues on the interior of the nascent beta-barrel wall with residues in one of the ext
25 ne buckling, followed by constriction of the nascent bud neck and ultimately ILV generation by vesicl
27 he obligate 3(S)-4(R) stereochemistry at the nascent C3-C4 bond of FBP requires a si-face attack by t
33 Gs arise during translation, we use ribosome-nascent chain (RNC) complexes of the electron transfer p
35 Ssb thus employs substrate-tailored dynamic nascent chain associations to coordinate co-translationa
37 control (QC) pathways that oversee mRNA and nascent chain destruction and recycle ribosomal subunits
41 generate dual-labeled proteins and ribosome-nascent chain libraries suitable for single-molecule FRE
42 w that SecA cotranslationally recognizes the nascent chain of an inner membrane protein, RodZ, with h
44 eal its working principle, we determined the nascent chain-binding pattern of Ssb at near-residue res
49 e found that Ltn1p efficiently accessed only nascent-chain lysines immediately proximal to the riboso
50 ind that rapid synthesis of misfolding-prone nascent-chain segments increases the fraction of folded
58 lipid with 12 carbon acyl chains, yielded a nascent conformer that decreased in abundance as a funct
59 e integration of GABAergic interneurons into nascent cortical circuits is critical for ensuring norma
60 tein-LPXT to the terminal Gly residue of the nascent cross-bridge of peptidoglycan lipid II precursor
61 inally, the recruitment of AIC components to nascent Cryptococcus-containing vacuoles (CnCVs) regulat
62 affected by MVM infection, the production of nascent cyclin B1 RNA was substantially diminished at la
65 clease activity of MRE11, and the fragmented nascent DNA accumulates in the cytosol, initiating an in
66 incoming capsid cores containing RNA and/or nascent DNA and follow reverse transcription kinetics.
67 ciency causes Mre11-dependent degradation of nascent DNA at stalled forks, leading to cell lethality.
68 1 and BRCA2 in preventing the degradation of nascent DNA by the MRE11 nuclease after replication stre
70 remodelers, including ZRANB3 and HLTF, cause nascent DNA degradation and genomic instability in BRCA1
73 y incorporation of oxidized nucleotides into nascent DNA followed by incomplete base excision repair
74 porated 5-ethynyl-2'-deoxyuridine (EdU) into nascent DNA in cells infected with vaccinia virus (VACV)
75 eal that delayed accumulation of H3K27me3 on nascent DNA is essential to recruit pioneer transcriptio
76 The association of the topoisomerases with nascent DNA was dependent on expression of the viral DNA
77 hemistry and iPOND (Isolation of proteins on nascent DNA) to investigate vaccinia virus (VACV), the p
78 th a focus on the nucleolytic degradation of nascent DNA, a process commonly referred to as fork rese
79 n, and a protein kinase were associated with nascent DNA, as were the DNA-dependent RNA polymerase an
85 nt the extension of neuropil glia around the nascent EB and BU, and analyze the relationship of prima
86 are tools and will thereby contribute toward nascent efforts at global data exchange for identifying
87 anscription in epithelial multipotent cells, nascent endocrine progenitors, and differentiating and m
88 nt of attenuation correlates positively with nascent eRNA expression, the act of intragenic enhancer
95 ichment of saccharides and inorganic ions in nascent fine (PM2.5) and coarse (PM10-2.5) SSA and the s
96 we analyse 1,205 cells from the epiblast and nascent Flk1(+) mesoderm of gastrulating mouse embryos u
100 eration, but preserving FVIIIa generation by nascent FXa, can support intrinsic pathway coagulation.
101 erence tomography (OCT) imaging can identify nascent geographic atrophy (nGA) in eyes with intermedia
102 ar binding of apolipoprotein A-I (apoA1) and nascent high-density lipoprotein (HDL) assembly is not w
107 that impaired retrograde transport uncouples nascent IMVs from the IEV membranes at the site of wrapp
108 chymal cells expressed Sema3a, while central nascent islet cells produced the semaphorin receptor neu
110 farnesylation of KRAS4B, but trafficking of nascent KRAS4B to the PM is incompletely understood.
111 yuridine conversion to abasic sites ahead of nascent lagging strand DNA synthesis and subsequent bypa
112 In contrast, although Pol delta contacts the nascent lagging strands of active and stalled forks, it
114 ep of LD formation, namely the conversion of nascent LDs to mature LDs, and define a molecular role f
117 plication fork structures, the presence of a nascent leading strand, modelling the effects of replica
120 es, and probably bilayer-spanning domains of nascent membrane proteins, exit the lateral gate and doc
121 ommitment cycle' prepares gene expression in nascent merozoites to initiate sexual development throug
125 the silencing effect is mediated through the nascent mRNA, followed by activity of histone methylatin
126 circuits are: (i) sRNAs can potentially bind nascent mRNAs in the nucleoid, and (ii) localization pat
130 istrones, yet it remains impossible to study nascent mtDNA transcripts quantitatively in vivo using e
131 equencing (GRO-seq and PRO-seq) and analyzed nascent mtDNA-encoded RNA transcripts in diverse human c
132 chaperone proteins that transiently protect nascent ncRNA ends from exoribonucleases, with partner p
134 pear on the floor plate to interact with the nascent neural network, and thereby trigger immediate pl
138 A plays an important role in stabilizing the nascent nucleosome during the process of histone H2A-H2B
141 on efficiency by secondary structures of the nascent peptide chain, we performed a comparative analys
142 Although ribosomal subunit dissociation and nascent peptide degradation are well-understood, the mol
143 atic and bactericidal macrolides bind in the nascent peptide exit tunnel of the large ribosomal subun
146 ion kinetics depend on steric constraints on nascent peptide side chains and that confined movements
150 ality control (RQC) complex detects aberrant nascent peptides that remain stalled in 60S ribosomal pa
151 l chemical probes that facilitate imaging of nascent peptidoglycan to demonstrate that during acid st
153 he site of phagocytic cups as well as to the nascent phagosomes that harbor Gal/GalNAc lectin and act
157 port the proposed deprotonation at C4 of the nascent polyketide by the catalytic His1345 and the role
159 ehydration of the beta-hydroxy groups of the nascent polyketide intermediates, DH10 acts in a long-ra
162 cond core RQC component, Rqc2p, modifies the nascent polypeptide by adding a carboxyl-terminal alanin
165 e (LLO) donor to the asparagine residue of a nascent polypeptide chain is catalyzed by an oligosaccha
166 plasmic Hsp70 that binds ribosome-associated nascent polypeptide chains, also binds to the TPR domain
167 cA was found to also bind ribosomes near the nascent polypeptide exit tunnel, but the function of thi
168 o-translationally when the N-terminus of the nascent polypeptide is still attached to the ribosome.
169 eam of the RodZ transmembrane domain dictate nascent polypeptide selection by SecA instead of the SRP
172 eins while avoiding the misidentification of nascent polypeptides and correctly folded proteins is li
173 ard-to-see protein subpopulations, including nascent polypeptides being translated and post-translati
174 ts uncover two separate pathways that target nascent polypeptides for Ltn1-Cdc48-mediated degradation
176 nel that translocates signal peptide-bearing nascent polypeptides into the endoplasmic reticulum (ER)
178 and identified mitotic regulators, misfolded nascent polypeptides, and pathological Huntingtin varian
179 We found that HeLa cell chromatin-associated nascent pre-mRNA (CA-RNA) contains many unspliced intron
180 xons before or soon after exon definition in nascent pre-mRNA, and while m(6)A is not required for mo
184 gers nucleolar stress, as shown by decreased nascent pre-rRNA synthesis, fibrillarin perinucleolar ca
186 s of different chaperone classes receive the nascent protein chain emerging from the ribosome and gui
187 se integral membrane proteins can divert the nascent protein chains to the ribosome-associated comple
189 gation schedules, can persist long after the nascent protein has been released from the ribosome, and
190 field of in vivo protein biophysics is that nascent-protein behavior is a type of nonequilibrium phe
191 inetics can be more important in determining nascent-protein behavior than the thermodynamic properti
194 earrangements required for proper folding of nascent proteins synthesized in the endoplasmic reticulu
195 ) enables the cell-type-specific labeling of nascent proteins with a non-canonical amino-acid and cli
196 in (Naa10p) catalyzes N-alpha-acetylation of nascent proteins, and mutation of human Naa10p is linked
197 mitochondrial and endoplasmic reticulum (ER) nascent proteins, supporting its general chaperone funct
200 lized the endothelial cell (EC) monolayer in nascent pulmonary vessels, thereby contributing to EC su
201 al processes provides a vesicle reservoir to nascent release sites, which contrasts to the gradual de
203 d a deep sequencing strategy to characterize nascent reverse transcription products and their precise
204 uires the nuclear import of approximately 80 nascent ribosomal proteins and the elimination of excess
210 We also report transcriptional "bursts" of nascent RNA from integrated proviral DNA, and concomitan
211 termination is caused by the formation of a nascent RNA hairpin adjacent to a weak RNA-DNA hybrid wi
213 t genes as measured by both steady-state and nascent RNA levels and perturbed embryonic stem cell dif
214 provide the first comprehensive analysis of nascent RNA profiles of mRNAs, primary micro-RNAs, long
216 RNA polymerase activity is regulated by nascent RNA sequences, DNA template sequences, and conse
219 ion is reprogrammed upon stress, we measured nascent RNA synthesis at nucleotide-resolution, and prof
222 ble elements and guide Argonaute proteins to nascent RNA transcripts to induce co-transcriptional gen
223 ng a ribozyme to co-transcriptionally cleave nascent RNA, we demonstrate that such a free RNA end cou
224 hat Rbfox1 bound predominantly to introns in nascent RNA, while cytoplasmic Rbox1 bound to 3' UTRs.
227 gle-cell approaches (allele-specific RNAseq, nascent RNA-fluorescent in situ hybridization and immuno
233 ntaining effector complexes to complementary nascent RNAs to initiate histone H3 lysine 9 di- and tri
235 f the mutants were affected for lysis of the nascent SCV or vacuolar replication in epithelial cells,
236 c organelle that matures a massive amount of nascent secretory polypeptides, is particularly sensitiv
237 echanistic changes in the growth zone and in nascent segments during the generation of individual seg
238 st to highly differentiated sex chromosomes, nascent sex chromosome pairs are homomorphic or very sim
239 polymorphic within species, suggesting that nascent sex chromosomes arise frequently over the course
242 Pol epsilon has greater contact with the nascent single-stranded DNA (ssDNA) of the leading stran
243 exchange reaction (PER) cascades, which grow nascent single-stranded DNA with user-specified sequence
247 teins cooperate to protect and chaperone the nascent snRNA during its journey to the spliceosome.The
249 PCNA binding, Srs2 also displaces Rad51 from nascent strands to prevent recombination-dependent repea
252 During ribosome biogenesis in eukaryotes, nascent subunits are exported to the cytoplasm in a func
254 ctivation increases dendritic complexity and nascent synapse number, but synapse maturation requires
255 zymes on the endoplasmic reticulum (ER), and nascent TAGs are sequestered in the acyl moiety region b
256 for membrane targeting and ubiquitination of nascent tail-anchored membrane proteins to understand ho
257 with New Zealand reportedly considering the nascent technology as a way to locally eliminate the mam
258 end processing and increased destruction of nascent TERC RNA transcripts, resulting in telomerase de
259 s study, we present our vision for how these nascent tools may transform our view of fundamental cell
260 that early auditory responses demonstrate a nascent topographic organization, suggesting that topogr
261 uired for most splicing, its addition in the nascent transcript is a determinant of cytoplasmic mRNA
262 nctional evolutionary approach that involves nascent transcript mapping in S. cerevisiae strains cont
263 ysis reveals that KOW5 directly contacts the nascent transcript, and deletion of KOW5 disrupts this i
267 if identification for DNase-seq, analysis of nascent transcription from Global-Run On (GRO-seq) data,
268 roliferating cells, methods to differentiate nascent transcription from preexisting mRNAs are desired
270 t neurons further revealed reductions in the nascent transcription of long genes and uncovered widesp
272 symmetric division, budding yeast keeps the nascent transcription rates of its RNA polymerases const
276 model for identifying active miRNA TSSs from nascent transcriptomes generated by global run-on sequen
278 embryos using dual-fluorescence detection of nascent transcripts containing 5' MS2 and 3' PP7 RNA ste
280 walk-on assay that semi-globally quantifies nascent transcripts in promoter-proximal paused RNA poly
281 ach allows us to distinguish Notch-dependent nascent transcripts in the nucleus from mature mRNAs in
282 he co-transcriptional processing and fate of nascent transcripts is coordinated by transcription elon
283 escent in situ hybridization with probes for nascent transcripts to determine how soon after uncoatin
284 small RNA pathways to compete for access to nascent transcripts undergoing co-transcriptional splici
285 hromatin patterns through the interaction of nascent transcripts with their DNA template via the form
291 p-binding complex (CBC) to the 5' end of the nascent U1 snRNA, which ultimately influences the utiliz
296 ly does BCA2 prevent assembly and release of nascent virions, it also significantly restricts HIV-1 t
297 s protein substrates in vitro and in vivo in nascent virus particles via one-dimensional diffusion al
298 ilization into the endoplasmic reticulum for nascent VLDL particle assembly activates CREBH processin
299 are believed to latch onto or fuse with the nascent VLDL particle in either the ER or the Golgi comp
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