ライフサイエンスコーパス: nascent peptide

1 of introducing a charged amino acid into the nascent peptide.

2 o control the membrane insertion of the same nascent peptide.

3 the non-coding gap region as well as of the nascent peptide.

4 nner, elongating rather than terminating the nascent peptide.

5 n ribosomal complexes that are devoid of the nascent peptide.

6 ected by the unique primary sequence of each nascent peptide.

7 ribosome exit tunnel and the sequence of the nascent peptide.

8 to monitor the sequence and structure of the nascent peptide.

9 of ribosome recognition of the features of a nascent peptide.

10 a helix by a single, distant mutation in the nascent peptide.

11 ific interactions between the tunnel and the nascent peptide.

12 TC, responding to the presence of a specific nascent peptide.

13 role in translation and secondary folding of nascent peptides.

14 e 60 (which eliminates part of the essential nascent peptide) also distorts these estimates.

15 understanding of the elements required for a nascent peptide and a small regulatory molecule to contr

16 regulated by the length and sequence of the nascent peptide and by the conformational state, detecte

17 some is able to monitor the structure of the nascent peptide and can stall in response to specific pe

18 doxical potential for interactions between a nascent peptide and eRF1 to obstruct the translation ter

19 Here, I discuss the nascent peptide and its tasks as it wends its way throug

20 tase, binds to ribosome after release of the nascent peptide and promotes dissociation of the class I

21 ested that specific interactions between the nascent peptide and the antibiotic in the ribosomal exit

22 lity establishes the spatial position of the nascent peptide and the oxyanion and places the amine ne

23 A(f) and tested for their incorporation into nascent peptides and full-length protein using an Escher

24 Tom1 interacts with aberrant nascent peptides and is essential to limit their accumul

25 ogether with the detection of intron-encoded nascent peptides and RPS6/RPL7-carrying complexes in the

26 Translation arrest directed by nascent peptides and small cofactors controls expression

27 RNA complex binds to the signal sequence of nascent peptides and then docks with FtsY at the membran

28 tem that is both large enough to accommodate nascent peptides, and that traverses the particle.

29 By analyzing nascent peptide- and antibiotic-dependent ribosome stall

30 10, whose mutation reduces the efficiency of nascent peptide- and antibiotic-dependent ribosome stall

31 tures of the amino acid sequence of the TnaC nascent peptide are recognized by the translating riboso

32 , which remove the initiator methionine from nascent peptides, are essential in all organisms.

33 menon that requires CAT-tail addition to the nascent peptides by Rqc2.

34 ading frame on tmRNA that tags the defective nascent peptide chain for degradation.

35 ongation phase, amino acids are added to the nascent peptide chain in accordance with codon sequences

36 upling between the stalling of the elongated nascent peptide chain in the ribosome and its insertion

37 neither specific amino acids nor a specific nascent peptide chain length was required for AAP to inh

38 on efficiency by secondary structures of the nascent peptide chain, we performed a comparative analys

39 critically involves physical partitioning of nascent peptide chains into the lipid bilayer.

40 urs, specifically whether it can begin while nascent peptide chains of individual subunits are still

41 , is a conserved and primary modification of nascent peptide chains.

42 To probe the role of nascent peptide charges in ribosome function, we used a

43 the structure of the exit tunnel and/or the nascent peptide configuration in the tunnel.

44 basis for understanding how the drug and the nascent peptide cooperate to inhibit peptide bond format

45 me-mediated degradation of apoB targets both nascent peptides cotranslationally before translocation

46 sis that polyamines and the structure of the nascent peptide create a rate-limiting step in uORF tran

47 exit tunnel, the C-terminal sequence of the nascent peptide, critical for stalling, is in the immedi

48 Although ribosomal subunit dissociation and nascent peptide degradation are well-understood, the mol

49 An extreme manifestation of such response is nascent peptide-dependent ribosome stalling, involved in

50 ing of the molecular mechanisms of drug- and nascent peptide-dependent ribosome stalling.

51 A2503 mutations abolish nascent-peptide-dependent stalling at the leader cistron

52 Kinetic studies show that some nascent peptides dramatically inhibit rates of peptide r

53 ne disclose side-chain rearrangements of the nascent peptide during chain elongation.

54 nter of the large subunit and the site where nascent peptides emerge.

55 g.Two of the bypassing signals, a cis-acting nascent peptide encoded by the first open reading frame

56 the second (a stretch of amino acids in the nascent peptide encoded by the first ORF) destabilizes p

57 The nascent peptide exit tunnel and peptidyltransferase cent

58 ocation of the macrolide binding site in the nascent peptide exit tunnel at some distance from the pe

59 f newly synthesized polypeptides through the nascent peptide exit tunnel of the bacterial ribosome.

60 atic and bactericidal macrolides bind in the nascent peptide exit tunnel of the large ribosomal subun

61 daptation of the exit tunnel for hydrophobic nascent peptides, extensive remodeling of the central pr

62 ucture and function, and in mRNA binding and nascent peptide folding.

63 olide antibiotic in the tunnel positions the nascent peptide for interaction with the tunnel sensory

64 ATPase activity of Hsp70 chaperones to bind nascent peptides for proper folding, translocation or ma

65 the clearance of ubiquitinated, tRNA-linked nascent peptides from ribosomes.

66 after the first transmembrane segment in the nascent peptide has emerged from the ribosome.

67 nce of an inducing antibiotic and a specific nascent peptide in the exit tunnel abrogate the ability

68 protein synthesis is most efficient when the nascent peptide in the ribosome carries an alanine resid

69 These data indicate that the TnaC nascent peptide in the ribosome exit tunnel interacts wi

70 osyl residue of the oligosaccharide chain of nascent peptides in the endoplasmic reticulum (ER).

71 Specific nascent peptides in the ribosome exit tunnel can elicit

72 es and outlines our current understanding of nascent peptide-induced ribosome stalling in regulating

73 Translation of the TnaC nascent peptide inhibits ribosomal activity in the prese

74 An ion channel protein begins life as a nascent peptide inside a ribosome, moves to the endoplas

75 A number of nascent peptides interact with the exit tunnel and stall

76 to ribosome forward movement, facilitated by nascent peptide interactions that disengage the ribosome

77 tional modification is to fine-tune ribosome-nascent peptide interactions.

78 Cotranslational insertion of the nascent peptide into the membrane brings the translating

79 ization of SecM-arrested ribosomes shows the nascent peptide is covalently linked via glycine 165 to

80 ion from transiently arrested to full-length nascent peptide is faster for peptides containing neutra

81 rates of initial peptide elongation steps as nascent peptide is formed.

82 roduce transient arrest (pausing) before the nascent peptide is fully elongated.

83 ester bond between the peptidyl tRNA and the nascent peptide is hydrolyzed.

84 In this mechanism, while the nascent peptide is still in the exit tunnel of the ribos

85 ed by both proteins synergistically, and the nascent peptide is transferred to the translocon.

86 bosomal exit tunnel, along with its resident nascent peptide, is no less susceptible to these consequ

87 study we show that opsin, within a range of nascent peptide lengths, targets and translocates equall

88 part of this conserved sequence acts at the nascent peptide level to stimulate the frameshifting, wi

89 ate in the termination process, the complete nascent peptide linked to the tRNA that decodes the fina

90 Nascent-peptide modulation of translation is a common re

91 el protein containing the C-terminal Asp-Pro nascent peptide motif (which interferes with translation

92 d at stop codons on full-length mRNA using a nascent peptide motif that interferes with translation t

93 n Escherichia coli, we identified additional nascent peptide motifs that stall ribosomes.

94 During this process, the nascent peptide navigates along 100A of tunnel from the

95 folding by reducing ribosome speed when the nascent peptide needs time to form and optimize the core

96 The results indicate that short nascent peptides of each of the three proteins are unrea

97 t a shift-prone site is enhanced by specific nascent peptide or mRNA context features.

98 e inducible and underscore the importance of nascent peptide recognition by the ribosome for generati

99 The molecular mechanisms of the nascent-peptide recognition and ribosome stalling are un

100 mental reactions, peptide bond formation and nascent peptide release, during the elongation and termi

101 degraded by the cytoplasmic exosome, and the nascent peptides remaining in stalled 60S exit tunnels a

102 y structure can be acquired early, while the nascent peptide resides within the ribosomal exit tunnel

103 blematic sequence motifs are confined to the nascent peptide residues in the peptidyl transferase cen

104 Whereas Ltn1 polyubiquitylates these nascent peptides, Rqc2 directs the addition of C-termina

105 ct in concert with the previously identified nascent-peptide sensor, A2062, in the ribosome exit tunn

106 This transition depends on nascent peptide sequence at specific tunnel locations.

107 These results show that a nascent peptide sequence can influence translation conti

108 bosomal frameshifting due to features of the nascent peptide sequence that include the penultimate ph

109 l molecule is recognized along with specific nascent peptide sequences as a composite structure that

110 ture and to respond functionally to specific nascent peptide sequences is a fundamental property of t

111 Certain nascent peptide sequences, when within the ribosomal exi

112 ion kinetics depend on steric constraints on nascent peptide side chains and that confined movements

113 Evidence is presented suggesting that the nascent peptide signal may stimulate bypassing by destab

114 Here, we report single-molecule imaging of nascent peptides (SINAPS) to assess translation in live

115 Several nascent peptides stall ribosomes during their own transl

116 ngal arginine attenuator peptide (AAP), as a nascent peptide, stalls the translating ribosome in resp

117 We propose that ubiquitination of the nascent peptide starts on the 80S and continues on the 6

118 creases to 60% with a natural, heterologous, nascent peptide stimulator.

119 The ability to monitor the nascent peptide structure and to respond functionally to

120 efficient bypassing is contained within the nascent peptide synthesized prior to takeoff.

121 ality control (RQC) complex detects aberrant nascent peptides that remain stalled in 60S ribosomal pa

122 system to elucidate the contribution of the nascent peptide, the drug, and the ribosome toward forma

123 The nascent peptides then pass through the exit tunnel befor

124 rt is a "folding vestibule" that permits the nascent peptide to compact and explore conformational sp

125 the presence of Trp12, are necessary for the nascent peptide to create a tryptophan binding/inhibitio

126 nse and respond to single side chains of the nascent peptide to induce local conformational changes.

127 e from the ribosome and translocation of the nascent peptide to tmRNA.

128 a showing cross-linking of the N terminus of nascent peptides to nucleotides of the 23 S ribosomal RN

129 en the RQC complex fails to deliver aberrant nascent peptides to the proteasome for degradation.

130 h this N-terminal coumarin of ribosome-bound nascent peptides was examined.

131 phobic probe, coumarin, at the N terminus of nascent peptides was investigated.

132 s modulated by the C-terminal segment of the nascent peptide, where the third-from-last residue plays

133 a Cdc48 hexamer is recruited to extract the nascent peptides, which are addressed to the proteasome

134 sing is the result of the interaction of the nascent peptide with components of the large ribosomal s

135 ns results from abnormal interactions of the nascent peptide with the erythromycin resistance methylt

136 clease, perhaps due to an interaction of the nascent peptide with the ribosome or the daaP mRNA.

137 arginine biosynthetic enzyme, functions as a nascent peptide within the ribosomal tunnel and negative

138 As a nascent peptide within the ribosome exit tunnel, it acts

139 The AAP acts as a nascent peptide within the ribosome tunnel to stall tran