ライフサイエンスコーパス: nascent polypeptide

1 is facilitated by direct ATP binding to the nascent polypeptide.

2 n tmRNA-mediated SsrA-peptide tagging of the nascent polypeptide.

3 of eRF1 and eRF3 in ensuring fast release of nascent polypeptide.

4 ition of a C-terminal degradation tag to the nascent polypeptide.

5 d to the NH2-terminal glycine residue of the nascent polypeptide.

6 tidyl-tRNA bond and release of the completed nascent polypeptide.

7 RF2) from the ribosome after release of the nascent polypeptide.

8 me and adds its aminoacylated alanine to the nascent polypeptide.

9 as assayed by tmRNA-mediated tagging of the nascent polypeptide.

10 sequence determinants encoded throughout the nascent polypeptide.

11 (SAD) domain in the carboxy terminus of the nascent polypeptide.

12 codons) may be more biased than the complete nascent polypeptide.

13 e ribosome translates a messenger RNA into a nascent polypeptide.

14 osome activity to structure acquisition by a nascent polypeptide.

15 anisms that degrade the partially translated nascent polypeptide.

16 al sequence by SecYEG, and elongation of the nascent polypeptide.

17 sponds by degrading both the message and the nascent polypeptide.

18 tailing enables robust ubiquitination of the nascent polypeptide.

19 lly characterized for its role in processing nascent polypeptides.

20 the context of the tag added at the ends of nascent polypeptides.

21 t lacked the ability to add formyl groups to nascent polypeptides.

22 e formation of the Gbetagamma dimer from its nascent polypeptides.

23 rmyl group from the N-terminal methionine of nascent polypeptides.

24 , resulting in N-terminal formylation of all nascent polypeptides.

25 (3)Man(9)GlcNAc(2) to asparagine residues on nascent polypeptides.

26 lecular chaperones to interact directly with nascent polypeptides.

27 charides onto certain asparagine residues of nascent polypeptides.

28 rolyl isomerase activity and associates with nascent polypeptides.

29 bstituted for C-terminal signal sequences in nascent polypeptides.

30 und to be the predominant chaperone bound to nascent polypeptides.

31 s with the ribosome to assist the folding of nascent polypeptides.

32 act with the general population of cytosolic nascent polypeptides.

33 rminal signal peptide sequences contained in nascent polypeptides.

34 gnificant role in chaperoning the folding of nascent polypeptides.

35 cetylates the N termini of a wide variety of nascent polypeptides.

36 in-biogenesis factors that guide the fate of nascent polypeptides.

37 h glycosylation of acceptor sites (NXT/S) in nascent polypeptides.

38 rate to couple proteasomes to ribosome-bound nascent polypeptides.

39 of Srp1 and Sts1 in degradation of misfolded nascent polypeptides.

40 ore slowly translated, and aggregation-prone nascent polypeptides.

41 osomes, assisting the maturation of emerging nascent polypeptides.

42 ding and post-translational modifications of nascent polypeptides.

43 and prevents misfolding of newly synthesized nascent polypeptides.

44 rst ribosome-associated entities to bind the nascent polypeptide after peptide bond formation.

45 riminately arresting the elongation of every nascent polypeptide after the synthesis of six to eight

46 e affects the early processing events of the nascent polypeptide, altering the efficiency of ER inser

47 f reactions that append a peptide tag to the nascent polypeptide and 'rescue' the ribosome.

48 e and promotes degradation of the incomplete nascent polypeptide and problematic mRNA.

49 Interactions between the nascent polypeptide and the ribosome exit tunnel represe

50 hdrawal, enhanced the expression of arrested nascent polypeptides and caused constitutive protein fol

51 eins while avoiding the misidentification of nascent polypeptides and correctly folded proteins is li

52 release the stalled ribosomes and target the nascent polypeptides and mRNAs for degradation.

53 Protein chaperones promote native folding of nascent polypeptides and refolding of misfolded species,

54 racterize tertiary structural transitions of nascent polypeptides and show that the first nucleotide-

55 of co-translationally active chaperones with nascent polypeptides and the resulting effects on peptid

56 ndoplasmic reticulum (ER) to fold and modify nascent polypeptides and to synthesize phospholipids for

57 ing process by repairing oxidatively damaged nascent polypeptides and unfolded proteins.

58 NA enters stalled ribosomes, adds Ala to the nascent polypeptide, and serves as a template to encode

59 and identified mitotic regulators, misfolded nascent polypeptides, and pathological Huntingtin varian

60 ccomplished, we probe the mechanism by which nascent polypeptides are accurately sorted between the m

61 Nascent polypeptides are degraded by the proteasome conc

62 plasmic reticulum (ER) channel through which nascent polypeptides are imported and from which malfold

63 ational translocation and N-glycosylation of nascent polypeptides are mediated by a ternary supramole

64 s are split into subunits and the 60S-housed nascent polypeptides are poly-ubiquitinated by Listerin.

65 le quality control mechanisms to ensure that nascent polypeptides are properly folded and mature prot

66 complex, whereas the corresponding aberrant nascent polypeptides are ubiquitinated by the E3 ligases

67 template, the translation machinery, or the nascent polypeptide arrest the ribosome during translati

68 ble for cotranslational glycosylation of the nascent polypeptide as it enters the lumen of the endopl

69 le for co-translational glycosylation of the nascent polypeptide as it enters the lumen of the endopl

70 identify the Schizosaccharomyces pombe (Sp) nascent polypeptide associated complex (NAC) as a potent

71 tor (SR) are required for targeting, and the nascent polypeptide associated complex (NAC) prevents in

72 Drosophila homolog of beta NAC, a subunit of Nascent polypeptide Associated Complex (NAC).

73 autoallergen Hom s 2, the alpha-chain of the nascent polypeptide-associated complex (alpha-NAC).

74 ICD-1 is the beta-subunit of the nascent polypeptide-associated complex (betaNAC) and con

75 he signal recognition particle (SRP) and the nascent polypeptide-associated complex (NAC) are release

76 It has recently been proposed that the nascent polypeptide-associated complex (NAC) contributes

77 Nascent polypeptide-associated complex (NAC) is probably

78 We find that the nascent polypeptide-associated complex (NAC) is required

79 Nascent polypeptide-associated complex (NAC) was initial

80 he signal recognition particle (SRP) and the nascent polypeptide-associated complex (NAC), respective

81 es ribosome-associated complex (RAC) but not nascent polypeptide-associated complex (NAC).

82 y spliced isoform of naca, which encodes the nascent polypeptide-associated complex alpha polypeptide

83 ve haematopoiesis due to a deficiency in the nascent polypeptide-associated complex alpha subunit (NA

84 of two genes encoding an archaeal homolog of nascent polypeptide-associated complex alpha subunit and

85 rgeting, it has therefore been proposed that nascent polypeptide-associated complex functions as a cy

86 perties of the ribosome bound chaperone NAC (nascent polypeptide-associated complex).

87 presence of all cytosolic factors, including nascent polypeptide-associated complex.

88 alternative signal peptidase cleavage of the nascent polypeptide at a primary site (Cys(-)(1)-Asn(1))

89 the amino acid becomes incorporated into the nascent polypeptide at the site of the PTC.

90 Akt, mTORC2 mediates phosphorylation of the nascent polypeptide at the turn motif (TM) site, Thr450,

91 ing processes facilitates quality control of nascent polypeptides at each stage of their maturation.

92 ard-to-see protein subpopulations, including nascent polypeptides being translated and post-translati

93 n (CTU) is a robust process, with 12%-15% of nascent polypeptides being ubiquitinated in human cells.

94 RP receptor, and rejection of ribosome-bound nascent polypeptides beyond a critical length.

95 the basis of their ability to coprecipitate nascent polypeptides, both before and after chemical cro

96 cond core RQC component, Rqc2p, modifies the nascent polypeptide by adding a carboxyl-terminal alanin

97 properties of the C-terminal residues of the nascent polypeptide can affect the rate of translation t

98 In E. coli, the SecM nascent polypeptide causes elongation arrest, while inte

99 pathway that targets the associated mRNA and nascent polypeptide chain (NC).

100 hored proteins requires translocation of the nascent polypeptide chain across the endoplasmic reticul

101 Interaction between the nascent polypeptide chain and the ribosomal exit tunnel

102 ating ribosome which interacts with both the nascent polypeptide chain and the ribosome.

103 to be added to the carboxyl terminus of the nascent polypeptide chain by cotranslational switching o

104 he SRP pathway: the ongoing synthesis of the nascent polypeptide chain by the ribosome.

105 biosynthesis on the ribosome, an elongating nascent polypeptide chain can begin to fold, in a proces

106 , presumably the exit site through which the nascent polypeptide chain emerges from the ribosome.

107 s located close to the tunnel from which the nascent polypeptide chain exits the ribosome.

108 folding constrains the conformations of the nascent polypeptide chain in a manner not experienced by

109 zers and observe simultaneous folding of the nascent polypeptide chain in real time.

110 iation observed is not due to insertion of a nascent polypeptide chain into the membrane.

111 e (LLO) donor to the asparagine residue of a nascent polypeptide chain is catalyzed by an oligosaccha

112 During co-translational folding, the nascent polypeptide chain is extruded sequentially from

113 s indicate that tunnel interactions with the nascent polypeptide chain might be relevant for the regu

114 nts that are similar to those imposed on the nascent polypeptide chain.

115 at silencing occurs before completion of the nascent polypeptide chain.

116 UAA, and UGA) and facilitates release of the nascent polypeptide chain.

117 the formyl group from the N terminus of the nascent polypeptide chain.

118 the C-terminus of the partially synthesized nascent polypeptide chain.

119 hesis and processed during elongation of the nascent polypeptide chain.

120 0s which interact with the newly synthesized nascent polypeptide chain.

121 n Rmt2 substrates is likely to take place on nascent polypeptide chains and that these substrates exi

122 trigger factor (TF) interacts directly with nascent polypeptide chains as they emerge from the ribos

123 ecruits the multifunctional soluble Hsc70 to nascent polypeptide chains as they exit the ribosome.

124 ar membranes, and ensuring proper folding of nascent polypeptide chains during protein translation.

125 cells, including cotranslational folding of nascent polypeptide chains during their synthesis by the

126 kinase (EF2K), which inhibits elongation of nascent polypeptide chains during translation.

127 longation pausing, roughly at the site where nascent polypeptide chains emerge from the ribosomal exi

128 y the signal recognition particle (SRP) when nascent polypeptide chains emerge from the ribosome.

129 bably the first cytosolic protein to contact nascent polypeptide chains emerging from ribosomes.

130 Signal peptide-bearing nascent polypeptide chains emerging from the ribosome ar

131 itylation and extraction of ribosome-stalled nascent polypeptide chains for proteasomal degradation.

132 s certain aberrant, translationally arrested nascent polypeptide chains for proteasomal degradation.

133 zes stop codons and catalyzes the release of nascent polypeptide chains from ribosomes.

134 ssential role in the folding and assembly of nascent polypeptide chains in the ER.

135 hat both subunits are in direct contact with nascent polypeptide chains on the ribosome and that both

136 l strategies that would allow domains within nascent polypeptide chains to modulate gene expression.

137 ponent of the chaperone machinery that binds nascent polypeptide chains upon their exit from the ribo

138 ween the active site of the STT3 protein and nascent polypeptide chains using an in vitro photocrossl

139 e transfected into C2C12 cells, and sites of nascent polypeptide chains were detected using the biars

140 Correct folding of nascent polypeptide chains within the ER is critical for

141 During protein synthesis, nascent polypeptide chains within the ribosomal tunnel c

142 plasmic Hsp70 that binds ribosome-associated nascent polypeptide chains, also binds to the TPR domain

143 ates, allowing for more efficient folding of nascent polypeptide chains, down-regulation of the UPR,

144 OP-puro forms covalent conjugates with nascent polypeptide chains, which are rapidly turned ove

145 f thioether (lanthionine) cross-links within nascent polypeptide chains, yielding macrocyclic protein

146 from the ribosome through an exit tunnel as nascent polypeptide chains.

147 ribosome, facilitating its interaction with nascent polypeptide chains.

148 s that remove the N-terminal methionine from nascent polypeptide chains.

149 forms the cotranslational N-glycosylation of nascent polypeptide chains.

150 nts addition of N-linked oligosaccharides to nascent polypeptide chains.

151 lar chaperones, which can be cross-linked to nascent polypeptide chains.

152 or GroEL to form a stable interaction with a nascent polypeptide co-translationally, we translated th

153 Here, we generated ribosome-bound nascent polypeptide complexes (RNCs) with different poly

154 re released from the ER membrane as ribosome-nascent polypeptide complexes.

155 on of the individual mRNA, ribosomal, and/or nascent polypeptide components, thereby clearing the cel

156 ted to signal cotranslational degradation of nascent polypeptides, cotranslational ubiquitylation occ

157 puromycin, which purges translocon pores of nascent polypeptides, creating additional empty pores.

158 slational transfer of high-mannose sugars to nascent polypeptides during N-linked glycosylation in th

159 cular chaperones interact with and stabilize nascent polypeptides during synthesis and/or translocati

160 through capture onto a solid surface of the nascent polypeptides during translation of synthetic or

161 Nascent polypeptides emerging from a translating ribosom

162 Nascent polypeptides emerging from the ribosome and not

163 e translational apparatus and associate with nascent polypeptides emerging from the ribosome.

164 ght to be the first protein to interact with nascent polypeptides emerging from the ribosome.

165 exit, providing a unique environment to the nascent polypeptides emerging from the ribosome.

166 Srp1 binds nascent polypeptides emerging from the ribosome.

167 the mRNA channel, the tRNA passage, and the nascent polypeptide exit tunnel contain Lmr-specific pro

168 cA was found to also bind ribosomes near the nascent polypeptide exit tunnel, but the function of thi

169 position the adjacent ligase domain near the nascent polypeptide exit tunnel.

170 As nascent polypeptides exit ribosomes, they are engaged by

171 large ribosomal subunit near the site where nascent polypeptides exit.

172 nt before the exit site, suggesting a unique nascent-polypeptide exit mechanism.

173 Hsp70 family, Ssb, previously implicated in nascent polypeptide folding and protein turnover, exhibi

174 selected translation reactions to target the nascent polypeptide for rapid proteolysis.

175 st that TF has a unique ability to sequester nascent polypeptides for a relatively prolonged period.

176 selected translating ribosomes to target the nascent polypeptides for degradation.

177 ts uncover two separate pathways that target nascent polypeptides for Ltn1-Cdc48-mediated degradation

178 (RQC) engages stalled ribosomes and targets nascent polypeptides for proteasomal degradation.

179 minate these transcripts and target arrested nascent polypeptides for proteasomal degradation.

180 es stop codons and promotes the release of a nascent polypeptide from tRNA on the ribosome.

181 reatment with puromycin (0.1-1 mm) to remove nascent polypeptides from ribosomes increases Ca(2+) lea

182 vo in a reduced ability of Sup45p to release nascent polypeptides from the ribosome at the non-permis

183 Its nascent polypeptide has a putative amino-terminal endopl

184 is achieved through a tiered system wherein nascent polypeptides have a chance to fold before becomi

185 We demonstrate and caution that nascent polypeptides have a propensity for binding many

186 itment, tRNA interaction, and exiting of the nascent polypeptide in Lmr must differ markedly from the

187 nd P/E hybrid-state tRNAs, and analysis of a nascent polypeptide in the exit tunnel.

188 Correct folding of a nascent polypeptide in the lumen of the endoplasmic reti

189 rotein that interacts with a wide variety of nascent polypeptides in Escherichia coli.

190 removal of the N-terminal formyl group from nascent polypeptides in eubacteria.

191 ed the direct interaction of chaperones with nascent polypeptides in the cytosol of mammalian cells b

192 llowed by the transfer of the core glycan to nascent polypeptides in the endoplasmic reticulum (ER).

193 lyzes the cotranslational N-glycosylation of nascent polypeptides in the endoplasmic reticulum in all

194 in cytoplasmic loops caused retention of the nascent polypeptides in the endoplasmic reticulum, all t

195 of dolichol-linked oligosaccharide chains to nascent polypeptides in the endoplasmic reticulum, consi

196 yme that catalyzes N-linked glycosylation of nascent polypeptides in the lumen of the endoplasmic ret

197 on motif found at the C terminus of abnormal nascent polypeptides in vivo.

198 Glc(3), 1a) to an asparagine side chain of a nascent polypeptide inside the lumen of the endoplasmic

199 ng of transmembrane domains begins after the nascent polypeptide integrates into the lipid bilayer or

200 Nascent polypeptides interact cotranslationally with a f

201 to the tunnel exit guaranteeing an efficient nascent polypeptide interaction.

202 ng UAG at these two positions may divert the nascent polypeptide into an alternative folding pathway

203 ex is important for efficient insertion of a nascent polypeptide into the translocation pore.

204 is essential for productive insertion of the nascent polypeptide into the translocation site, channel

205 ins may play a critical role in transforming nascent polypeptides into 3-dimensional configurations t

206 sp70s, which assist in the proper folding of nascent polypeptides into higher ordered structures.

207 Timely and accurate selection of nascent polypeptides into the correct pathway is essenti

208 n topology by cotranslationally partitioning nascent polypeptides into the cytosol, ER lumen, and lip

209 nel that translocates signal peptide-bearing nascent polypeptides into the endoplasmic reticulum (ER)

210 global protein synthesis, lowering influx of nascent polypeptides into the stressed ER, coincident wi

211 In this process, the nascent polypeptide is modified by the addition of a sho

212 rmylated methionine, the formyl group of the nascent polypeptide is removed by peptide deformylase.

213 o-translationally when the N-terminus of the nascent polypeptide is still attached to the ribosome.

214 , and EF-TucGTP binds stalled ribosomes, the nascent polypeptide is transferred to the alanine on tmR

215 eria) that recognizes the signal sequence of nascent polypeptides is a GTPase, as is the SR-alpha sub

216 The interaction between hsp70 and nascent polypeptides is apparently dynamic under physiol

217 inefficient, and a substantial proportion of nascent polypeptides is rejected by an ER quality contro

218 t ribosome-translocon junction; synthesis of nascent polypeptides lacking a signal sequence resulted

219 The biosynthetic processing of the nascent polypeptide leading to channel assembly involves

220 ring by default in the absence of productive nascent polypeptide-membrane interactions.

221 ane-derived complexes, we determined how the nascent polypeptide modulates translocon component assoc

222 hese results indicate that ubiquitination of nascent polypeptides occurs in two contexts and define C

223 initiator Met in good Kozak context, a large nascent polypeptide of 3576 amino acids is predicted, in

224 s prolyl-isomerase activity, associates with nascent polypeptides on ribosomes, binds to GroEL, enhan

225 cribed additional components in contact with nascent polypeptides, our data indicate that the hsp70 a

226 As translation proceeds, nascent polypeptides pass through an exit tunnel that tr

227 e show that GP116 is synthesized as a 52-kDa nascent polypeptide precursor (p52) which is processed t

228 Nascent/newly synthesized proteins, nascent polypeptides released from the ribosome by purom

229 eam of the RodZ transmembrane domain dictate nascent polypeptide selection by SecA instead of the SRP

230 Analysis of the nascent polypeptides showed that the modifications at th

231 ultivalent fluorescence amplification of the nascent polypeptide signal, we develop a method to image

232 eveal that S-palmitoylation is important for nascent polypeptide stability of both proteins.

233 res composed of a dense core inaccessible to nascent polypeptides surrounded by a surface that stably

234 d with ribosomes and is required for optimal nascent polypeptide synthesis.

235 showed that while the hybrid tmRNA supported nascent polypeptide tagging and ribosome rescue, it suff

236 ) transfers high mannose-type glycans to the nascent polypeptides that are translated by the membrane

237 ds to 60S ribosomal subunits to ubiquitylate nascent polypeptides that become stalled during synthesi

238 Nascent polypeptides that emerge vectorially from the ri

239 alyses demonstrated that translocon-targeted nascent polypeptides that subsequently stall are polyubi

240 th rates and defects in the translocation of nascent polypeptides that use the cotranslational transl

241 erplay between biophysical properties of the nascent polypeptide, the vectorial nature and rate of tr

242 Since molecular chaperones often fold nascent polypeptides through a bind-and-release interact

243 to occur with the N-terminal segment of the nascent polypeptide to facilitate its folding on ribosom

244 tein and the envelope protein E1 targets the nascent polypeptide to the endoplasmic reticulum (ER) me

245 0 chaperone Ssb interacts with ribosomes and nascent polypeptides to assist protein folding.

246 d environment where numerous factors contact nascent polypeptides to influence their folding, localiz

247 es to detect the proximity of ribosome-bound nascent polypeptides to Sec61alpha.

248 omes stalled on truncated mRNAs and tags the nascent polypeptides to target them for proteolysis.

249 t signal peptides act primarily in targeting nascent polypeptides to the endoplasmic reticulum, we su

250 ence of an alternative route for delivery of nascent polypeptides to the proteasome.

251 challenged to efficiently and properly fold nascent polypeptides, traffic them to their appropriate

252 ing translation are split into subunits, and nascent polypeptides trapped in the 60S subunit are ubiq

253 the early stage of a protein's life, as the nascent polypeptide traverses and emerges from the ribos

254 ar basis of how hydrophobic signals within a nascent polypeptide trigger channel opening is not under

255 ER quality control" prevent the transport of nascent polypeptides until they properly fold.

256 uggests that the E3 ligase listerin accesses nascent polypeptides via a gap in the ribosome-transloco

257 ing ribosome docking on the ER membrane, the nascent polypeptide was shielded from the cytosol as it

258 tagged with biotin or BODIPY; although most nascent polypeptides were cytoplasmic, some were found i

259 iggers release of 60S subunits with attached nascent polypeptides, which undergo ubiquitination by th

260 We find that SSB binds to a subset of nascent polypeptides whose intrinsic properties and slow

261 oration of the coumarin derivative generated nascent polypeptides with a hydrophobic residue at their

262 to tag and capture a population of truncated nascent polypeptides with no bias as to the identity of

263 the folding of single ribosome-bound stalled nascent polypeptides with optical tweezers.

264 hat the cargo for SRP--ribosomes translating nascent polypeptides with signal sequences--accelerates

265 rimary and likely secondary structure of the nascent polypeptide within the ribosome exit tunnel can

266 eta cells beyond the capacity for folding of nascent polypeptides within the endoplasmic reticulum (E