ライフサイエンスコーパス: native chemical ligation

1 e beta-functionalized cysteines suitable for native chemical ligation.

2 ead-to-tail cyclization of peptides has been native chemical ligation.

3 de novo formation of synthetic liposomes via native chemical ligation.

4 cosylated forms of (Ser-)CCL1, by convergent native chemical ligation.

5 ncorporated into full-sized proteins through native chemical ligation.

6 rs them to a peptide-elongation site through native chemical ligation.

7 interfacing of sortase-mediated ligation and native chemical ligation.

8 -terminal cysteine-containing constructs via native chemical ligation.

9 was driven by genetically encoded orthogonal native chemical ligation.

10 strategically placed thiol groups, enabling native chemical ligation.

11 N-terminus of the IgG1 Fc glycoprotein using native chemical ligation.

12 our synthetic peptide segments by sequential native chemical ligation.

13 ombination of expressed protein ligation and native chemical ligation.

14 phase peptide synthesis in combination with native chemical ligation.

15 r synthetic zinc finger proteins produced by native chemical ligation.

16 thesis of proteins and of cyclic peptides by native chemical ligation.

17 ced with cysteine to facilitate synthesis by native chemical ligation.

18 EPO even without recourse to cysteine-based native chemical ligation, a concise synthesis of the par

19 fined orientation, as determined directly by native chemical ligation/affinity cleavage; by contrast,

20 genetically directed incorporation of 7 with native chemical ligation and desulfurization to yield an

21 and highlights the potential application of native chemical ligation and expressed protein ligation

22 agnitude faster reaction in model studies of native chemical ligation and in the synthesis of a small

23 MPAA should find broad use in native chemical ligation and in the total synthesis of p

24 Native chemical ligation and intein-based methods allow

25 ptide synthesis and assembled using combined native chemical ligation and isonitrile-mediated activat

26 Aided by native chemical ligation and mirror image phage display,

27 Using native chemical ligation and orthogonal protection of se

28 Three sequential cycles of native chemical ligation and subsequent thiyl radical re

29 The sequential combination of native chemical ligation and thiol-ene radical chemistry

30 ein, we report total chemical synthesis, via native chemical ligation, and functional characterizatio

31 man MDM2 and MDMX chemically synthesized via native chemical ligation, and identified several peptide

32 ease of producing C-terminal thioesters for native chemical ligation applications.

33 The thioester-mediated native chemical ligation approach mimics, to some extent

34 Here we extend the utility of this native chemical ligation approach to non-cysteine contai

35 ogue, N33A, synthesized by a newly developed native chemical ligation approach.

36 strategies relying on coupling reagents and native chemical ligation are available, there is a conti

37 The feasibility of native chemical ligation at -Gln-Cys- enabled a converge

38 We have re-examined the utility of native chemical ligation at -Gln/Glu-Cys- [Glx-Cys] and

39 ys- is not appropriate for use as a site for native chemical ligation because of formation of signifi

40 sine at different regions of alpha-syn using native chemical ligation combined with a novel desulfuri

41 that is analogous to the role of cysteine in native chemical ligation, combining chemoselective thioe

42 Our method has customized native chemical ligation concept for glycoconjugates syn

43 gments and examined their compatibility with native chemical ligation conditions.

44 ing its complete removal within 15 min under native chemical ligation conditions.

45 catalyst 4-mercaptophenylacetic acid (MPAA), native chemical ligation could be performed at -Gln-Cys-

46 Solid-phase native chemical ligation/cyclization methodology appears

47 nner complementary to that with conventional native chemical ligation-desulfurization strategies.

48 tag at the C-terminus was straightforward by native chemical ligation followed by conversion of the C

49 Native chemical ligation followed by desulfurization is

50 bled from four polypeptide segments by using native chemical ligation in a fully convergent fashion.

51 conditions; (viii). on-resin cyclization by native chemical ligation in an aqueous milieu; and (ix).

52 cyclic peptide bearing a thioester handle in native chemical ligation is shown by a high yielding lig

53 Application of native chemical ligation logic to the case of an N-termi

54 staples allowed us to use this motif in the native chemical ligation-mediated synthesis of a small p

55 rminal analogues of CCL-19 with the aid of a native chemical ligation method to investigate structure

56 lly (15)N-labeled analogue, Vpu(2-81), using native chemical ligation methodologies and also report a

57 Native chemical ligation (NCL) affords an N-linked chito

58 Although native chemical ligation (NCL) and related chemoselectiv

59 Successful operation of the machine via native chemical ligation (NCL) demonstrates that even ch

60 The broad utility of native chemical ligation (NCL) in protein synthesis has

61 Native chemical ligation (NCL) is widely applicable for

62 r structures to proteins was performed using native chemical ligation (NCL) of a C-terminal peptide t

63 into a peptide sequence in order to perform native chemical ligation (NCL) of two peptide fragments.

64 enerate modular tension probes combining the native chemical ligation (NCL) reaction with solid phase

65 igated to segment Asp(1)-Asn(36) by means of native chemical ligation (NCL) to give the full sequence

66 Here we describe the use of native chemical ligation (NCL) to rapidly prepare phosph

67 exchanged by a chemoselective and reversible native chemical ligation (NCL) which can take place at N

68 In one approach, native chemical ligation (NCL), short, unprotected pepti

69 g to a 38-residue leader peptide by means of native chemical ligation (NCL).

70 ration of a cysteine residue was achieved by native chemical ligation (NCL).

71 tilizing a semisynthetic scheme that employs native chemical ligation of a pentapeptide (HCDLP) to re

72 full-length alphaHL monomer was obtained by native chemical ligation of the central synthetic peptid

73 this problem, we synthesized crambin by the native chemical ligation of three segments (15 + 16 + 15

74 fficacy of chemical protein synthesis by the native chemical ligation of three segments and establish

75 synthesized the 46 amino acid polypeptide by native chemical ligation of two distinct sets of peptide

76 rotein was prepared by total synthesis using native chemical ligation of unprotected peptide segments

77 Native chemical ligation of unprotected peptide segments

78 applicability of total protein synthesis by native chemical ligation of unprotected peptide segments

79 This strategy, combined with native chemical ligation, provides convergent and rapid

80 The native chemical ligation reaction has been used extensiv

81 d a chemically synthesized peptide using the native chemical ligation reaction.

82 urther to produce covalently linked PNTs via native chemical ligation, rendering ca. 100 nm-long nano

83 iques, namely solid phase peptide synthesis, native chemical ligation, Staudinger ligation, NCA polym

84 to rapidly analyze the compatibility of the native chemical ligation strategy for X-Cys ligation sit

85 We introduce a sequential native chemical ligation strategy that is suitable for t

86 and functional role of this mark, we used a native chemical ligation strategy to generate histone H4

87 l glycopeptide fragments involved a stepwise native chemical ligation strategy to provide the longest

88 ans-cyclooctene, oxime, reductive amination, native chemical ligation, Suzuki, Sonogashira, cross-met

89 Using the native chemical ligation technique, we synthesized the p

90 The introduction of native chemical ligation techniques to join unprotected

91 se peptide synthesis based on an adaption of native chemical ligation technology and recombinant DNA

92 A novel auxiliary-mediated native chemical ligation that enables peptide ligation t

93 (alpha)thioester; this segment is joined by native chemical ligation to a 66-aa Cys peptide, to yiel

94 To explore its role in catalysis, we used native chemical ligation to generate semi-synthetic SrtA

95 ino acid peptide via Lansbury aspartylation, native chemical ligation to join peptide 19 with the gly

96 We used native chemical ligation to synthesize the grafted cycli

97 Here we describe the application of native chemical ligation to the preparation of full-leng

98 (Pg-alpha-aminoacyl)cysteines 4a,b underwent native chemical ligations to form native dipeptides 3f,i

99 n sequence, featuring up to 20-membered ring native chemical ligation transition states.

100 s of OMTKY3 with several serine proteinases, native chemical ligation was used for the total synthesi

101 ombination of expressed protein ligation and native chemical ligation was used to attach these analog

102 Using native chemical ligation, we have linked the Lys-D-Ala-D

103 Aided by mirror image phage display and native chemical ligation, we have previously discovered

104 ospecific track, oxytocin was constructed by native chemical ligation, wherein the two building block

105 Native chemical ligation, which is widely used in the sy

106 ve strategic fragments to be assembled using native chemical ligation with a focus on maximal converg

107 Following native chemical ligation with a thioester-containing seg

108 ry-modified peptides can be directly used in native chemical ligations with peptide thioesters easily