ライフサイエンスコーパス: native protein

1 apoptotic cells at a level comparable to the native protein.

2 experiments on the same mutant could produce native protein.

3 tepwise pathway that sequentially builds the native protein.

4 s for endogenous esterases, which regenerate native protein.

5 nism involves extensive clearance of the non-native protein.

6 of unknown function that is cleaved from the native protein.

7 native-like structural elements to build the native protein.

8 d more dichlorogenic acids compared with the native protein.

9 mprove the thermostability of a well-behaved native protein.

10 the rapid misfolding and aggregation of the native protein.

11 has a quaternary structure distinct from the native protein.

12 de and deleting the different domains of the native protein.

13 trans form of the surfactant relative to the native protein.

14 +/- 0.004) of E181Q relative to that of the native protein.

15 and induces antibodies against the unaltered native protein.

16 ng and enhances the overall stability of the native protein.

17 esidue-resolved solvent accessibility of the native protein.

18 DnaB is depleted at the oriC relative to the native protein.

19 iciting antibodies that cross-react with the native protein.

20 ence polypeptide also approaches that of the native protein.

21 e primase activity more efficiently than the native protein.

22 radius only slightly larger than that of the native protein.

23 performed on 729 near-native decoys for each native protein.

24 stant in sequence but nearly adjacent in the native protein.

25 largely retains the essential folding of the native protein.

26 tuted Gt that assembles differently than the native protein.

27 the way that the foldons fit together in the native protein.

28 nHCl, is approximately 3.6 times that of the native protein.

29 cine (Cys-Gly) with the same kinetics as the native protein.

30 ric and trimeric products in contrast to the native protein.

31 solution using the anomalous signal from the native protein.

32 hospholipid bilayers similar to those of the native protein.

33 ecombinant protein as well as for the mature native protein.

34 find it to be nearly isostructural with the native protein.

35 s, they are extremely difficult to purify as native protein.

36 tivity different from that of the metal in a native protein.

37 mic mRNAs, in the presence or absence of the native protein.

38 effective as antibodies directed against the native protein.

39 self-antigen, rather than a response to the native protein.

40 ns of cysteines or lysines, respectively, in native proteins.

41 ropagating aggregation of nominally healthy, native proteins.

42 purification yield and authentic folding for native proteins.

43 P and convert stable protein aggregates into native proteins.

44 eins and conferring increased stability upon native proteins.

45 d heme c proteins as reliable models for the native proteins.

46 ized protein oligomers generated from common native proteins.

47 dependence of local protein unfolding within native proteins.

48 ities that open and close to encapsulate non-native proteins.

49 ique method to enhance the immunogenicity of native proteins.

50 der physiologically relevant conditions with native proteins.

51 nanoreactors for single-molecule studies of native proteins.

52 rts different chemical reactivities than the native proteins.

53 etabolite immobilization and incubation with native proteins.

54 residues on the solvent-exposed surfaces of native proteins.

55 purified recombinant proteins in adjuvant as native proteins.

56 ydrophobic segments across the length of non-native proteins.

57 reby maintaining the cellular pool of active native proteins.

58 s protease-degradable or biologically active native proteins.

59 use ATP to dissociate and convert them into native proteins.

60 ein research, in particular for the study of native proteins.

61 morphic residues, which significantly affect native protein activity, structure, or function and are

62 -related and neurological diseases, formerly native proteins aggregate via formation of a partially u

63 scape, which results in the formation of non-native protein aggregates that challenge the capacity of

64 tm1-Pdi1 complex processes both unfolded and native proteins albeit with a preference for the former.

65 This approach adopts native proteins (albumin) as bio-receptors that interact

66 essed at substantially lower levels than the native protein, allowing assessment not only of the lack

67 Degradation of specific native proteins allows bacteria to rapidly adapt to chan

68 Applications ranging from native protein analyses to molecular library constructio

69 duce inaccurate concentration assignments of native protein analytes by bottom-up analysis due to var

70 ng molecular dynamic simulations of both the native protein and a realistic denatured state ensemble.

71 is antigenically indistinguishable from the native protein and forms a homogeneous trimer in solutio

72 specially viscera, as a source for obtaining native protein and hydrolysates, explaining their produc

73 omain, induces antibodies that recognize the native protein and interfere with S. mutans adhesion in

74 ct of this insertion on the structure of the native protein and its folding mechanism were studied us

75 d that it consisted of a mixture of refolded native protein and partially folded protein and that the

76 nd to exhibit biophysical characteristics of native protein and reacted with native-OVA specific mono

77 -ThioFab retained the biological activity of native protein and was successfully validated in vivo wi

78 d into Escherichia coli and expressed as the native protein and with an N-terminal His(6) tag.

79 detailed protocols for the immunization with native proteins and for the selection by phage display o

80 o-clustering with other existing clusters of native proteins and lipids in the membrane.

81 ptides show higher antioxidant capacity than native proteins and may be absorbed in the intestine wit

82 aperone-mediated kinetic partitioning of non-native proteins and may help explain the etiology of sep

83 pair facilitates the study of RNA binding to native proteins and peptides, which is demonstrated here

84 release agent able to produce nonaggregated native proteins and preserve the overall nature of the m

85 potential valuable tools for the MS study of native proteins and protein complexes.

86 d pairs in close proximity on the surface of native proteins and protein complexes.

87 For native proteins and their complexes, many structural fea

88 ergy of ATP binding and hydrolysis to unfold native proteins and translocate unfolded polypeptides in

89 [(32)P]c-di-GMP bound several E. chaffeensis native proteins and two E. chaffeensis recombinant I-sit

90 Our work demonstrates the malleability of "native" proteins and implies that crowding-induced shape

91 ror image forms of sfAFP (i.e., L-sfAFP, the native protein, and D-sfAFP, the native protein's enanti

92 nfolded protein are slower than those of the native protein, and the unfolded protein fast dynamics a

93 periments show that the fast dynamics of the native protein are virtually temperature independent.

94 Conformational change and aggregation of native proteins are associated with many serious age-rel

95 Such studies have found that native proteins are brittle, and thus not very deformabl

96 nd to be unrealistic because the dynamics of native proteins are dominated by subglobal transitions a

97 Many native proteins are multi-specific and interact with num

98 Active sites and ligand-binding cavities in native proteins are often formed by curved beta sheets,

99 ese proteins were tested to determine if the native proteins are Tat exported.

100 -terminal regions (approximately half of the native protein) are phenotypically near-normal; and thos

101 at commonly used haptenated proteins, unlike native proteins, are inherently immunogenic.

102 lution (experimental K(d)=17 microM) and the native protein as the second best solution (experimental

103 he calculation selects a point mutant of the native protein as the top solution (experimental K(d)=17

104 -down proteomics experiments, especially for native protein assemblies.

105 mmalian expression system where the rules of native protein assembly are strictly obeyed.

106 s are, however, distinct from those with the native protein because the metal complex occupies the su

107 Thus, helices, in contrast with native proteins, become more stable with increasing pres

108 rol (QC) activity in the partitioning of non-native proteins between life and death are not clear.

109 folded fragments, but it is not clear if the native protein binds fibronectin with the same primary s

110 d terminal cysteinate were suggested for the native protein but have not yet been confirmed experimen

111 In conclusion, the native proteins (but not the nucleic acids, lipids or ca

112 Mcl1 is present at levels comparable to the native protein, but it is markedly stabilized in cells s

113 trates in the first solvation shell of quasi-native proteins, but with a density lower than that of t

114 agged PSBS that were exploited to purify the native protein by affinity chromatography.

115 s tryptic digestion and gradual unfolding of native proteins by application of a temperature gradient

116 ism by which Hsp90 can recognize and remodel native proteins by binding and remodeling partially fold

117 ction was confirmed by GST pulldown and, for native proteins, by co-immunoprecipitation experiments i

118 ated with a smaller protein complex than the native protein complex associated with endogenous SHB1.

119 a slightly smaller protein complex than the native protein complex, but it largely displaced endogen

120 t largely displaced endogenous SHB1 from its native protein complex.

121 MPC-iron complex than native protein complex.

122 stoichiometry and composition of endogenous native protein complexes at an unprecedented level of de

123 approach allows the biochemical isolation of native protein complexes for proteomic studies.

124 gmentation approach, or (pseudo)-MS(3), from native protein complexes to a set of constituent fragmen

125 hogonal handles for biochemical isolation of native protein complexes.Split fluorescent proteins (FPs

126 Mass spectrometry analysis of the native protein confirmed the post-translational modifica

127 We also show that native protein conformation is conserved in TENG-ESI, an

128 The key insight is that the search for the native protein conformation is influenced by the rate r

129 tein microarray such as covalent attachment, native protein conformation, homogeneity of the protein

130 a unique capacity to bind and stabilize non-native protein conformations, prevent aggregation, and k

131 Non-native protein conformers generated by mutation or chemi

132 n Wbl research is to obtain and characterize native protein containing iron-sulfur clusters.

133 e or crypticity of a peptide observed in its native protein context is preserved.

134 he hypothesis explored in this work was that native proteins could be digested and identified without

135 scription factors (NANOG, MYOD), antibodies, native proteins (cytochrome C), magnetic nanoparticles (

136 Starting from the native Protein Data Bank structure, nine (meta)stable st

137 ut their kinetic profiles are different; the native protein did not show any such tendency under the

138 Understanding how a single native protein diffuses on its free-energy landscape is

139 ed in a three-state model where cross-linked native protein dimer, N-N, interconverts in a two-state

140 dditional structures were determined for the native protein (dmin = 2.0 A) as well as binary complexe

141 nding affinity prediction, discrimination of native protein-DNA complex from decoy structures, and mo

142 kinase are not affected by loop elongation, native protein dynamics that are essential for efficient

143 of intramolecular signaling and catalysis to native protein dynamics that arise from modest changes i

144 ibrils was measured in vitro and compared to native protein, early-stage-fibrillar protein, and sonic

145 ous insert were positioned in frame with the native protein-encoding sequences but were separated fro

146 chanical disruption of a metal center in its native protein environment in aqueous solution.

147 hese chemically identical molecules in their native protein environment.

148 recapitulates the self-assembly mechanism of native protein fibrils in which a ligand binding event g

149 eating sites for amyloid growth and, as with native protein folding, appear important for backbone de

150 appropriate micellar environment to preserve native protein folding.

151 ure modulates ribosome elongation to promote native protein folding.

152 chaperonin GroES to that ring ejects the non-native protein from its binding sites, through forced un

153 hogenesis is mediated by misappropriation of native protein function, a mechanism that may apply broa

154 Native protein gel analysis revealed that transgenic Hsp

155 ne)-sulfate, that detects enzyme activity in native protein gels, allowing the rapid detection of sul

156 tte of reaction chemistries in re-engineered native proteins has proved challenging.

157 etween modular domains within the context of native proteins have been largely unexplored.

158 bstitutions rarely increase the stability of native proteins; hence, large libraries and high-through

159 c peptides are built by assembling 7-residue native-protein heptad modules into new combinations.

160 screening the quaternary organization of 60 native proteins identified numerous discrete supercomple

161 ppear to work through the same mechanisms as native protein immunotherapy.

162 tions among different influential factors in native proteins impede progress toward complete understa

163 he small number of representative pockets in native proteins implies that promiscuous interactions ar

164 ng ALA protein was less immunogenic than the native protein in rabbits.

165 uent uptake of fibrils that directly contact native protein in recipient cells.

166 t acted as a dominant negative retaining the native protein in the endoplasmic reticulum.

167 surface versus the dynamics displayed by the native protein in the hydrated solid state.

168 The remaining native protein in turn can denature in another two-state

169 yte-filled nanopores can characterize single native proteins in an aqueous environment, but currently

170 sHsps complex with a variety of non-native proteins in an ATP-independent manner and, in the

171 dily usable protocol for the transduction of native proteins in C. elegans, which is based on the enc

172 enabled the detection of UAG readthrough in native proteins in E. coli strains in which UAG was reas

173 Model peptides in buffer as well as native proteins in human blood plasma additionally exhib

174 rapid efficient photodeprotection to reveal native proteins in live cells.

175 Molecular chaperones act on non-native proteins in the cell to prevent their aggregation

176 xM method did not result in the retention of native proteins in the gel and relied on custom-made rea

177 efined compounds in binding assays employing native proteins in their normal cellular location or con

178 se machine that rescues various forms of non-native proteins including the highly resistant amyloid f

179 nd binding assays with peptides derived from native proteins indicated that these two PDZ domains hav

180 e with emerging evidence that alterations in native protein interactions contribute to toxicity.

181 re of oligomers and fibrils sequestrates the native protein into an inactive conformation that is typ

182 can induce the conformational conversion of native proteins into aggregates that can be transmitted

183 We incorporated the native proteins into membrane vesicles and reconstituted

184 out" experiments, where the concentration of native protein is compared both with and without the pre

185 sured for pure lysozyme, suggesting that the native protein is dormant on the nanosecond time and nan

186 osphorylated FadD32 isoforms showed that the native protein is phosphorylated by serine/threonine pro

187 ions and post-translational modifications of native proteins is a challenge for research and diagnost

188 mation between aldehydes and amino groups in native proteins is markedly disfavored due to protonatio

189 des or other linear substrates compared with native proteins is most likely related to the reduced ac

190 The oxidatively damaged, but not the native protein, is a substrate of the Pln protease.

191 ts may be compromised by the presence of non-native protein junctions that result in protein misfoldi

192 ant protein with sequence identical with the native protein lacking the N-terminal pyroglutamate (the

193 quantify post-translational modifications at native protein level in order to correlate their influen

194 to characterize the sequences of proteins in native protein-ligand and protein-protein complexes and

195 ID), for the top-down MS characterization of native protein-ligand and protein-protein complexes.

196 Benchmark tests on 8 native protein-ligand complexes show that the method can

197 are also successfully modelled, reproducing native protein-ligand contacts with significantly differ

198 optimize the quantified specificity of the "native" protein-ligand complex discriminating against "n

199 ingle and mixed polymers, and self-assembled native protein-lipid complexes (Nanodiscs).

200 we investigate how the reverse-sequences of native proteins might fold by examining a series of smal

201 nm and 8.8 in water to 446 nm and 2.8 in the native protein milieu, respectively, by protein-ligand i

202 The interaction of Hsp40s with native proteins modifies their structure and function.

203 xed disulfides between N' and glutathione to native protein (N).

204 Native proteins often lack immunogenicity and thus limit

205 of DMSO can reduce in-source dissociation of native protein oligomers and their interactions with hyd

206 s approach can be used to make inhibitors of native proteins, or to develop novel peptides for applic

207 ability of c-Jun to heterodimerize with its native protein partner, c-Fos, and therefore designed a

208 acokinetics and therapeutic effects over the native protein/peptide upon administration to mice.

209 itous chaperones that bind and sequester non-native proteins preventing their aggregation.

210 Besides inducing misfolding of native proteins, prions bind nucleic acids and other pol

211 divergence was observed in the reporter and native protein profiles depending on the difference in t

212 Native protein-protein binding was confirmed by co-immun

213 icity which determines the discrimination of native protein-protein complex against competitive ones.

214 trated that the interfaces of native and non-native protein-protein complexes can be distinguished us

215 ation of this strategy to a more challenging native protein-protein interaction (PPI) suggested that

216 Top-down MS of the native protein provides complementary sequence informati

217 valuating the extent of glycosylation of the native proteins, providing valuable information when the

218 (1) the primary reference material BCR457-a native protein purified from human thyroids, (2) a comme

219 d in the recognition of dimethyl arginine by native protein receptors.

220 Reminiscent of the native protein, recombinant ZP3R/sp56 formed a high mole

221 eneration and polar recruitment, whereas the native protein recruits T-complexes to cell poles indepe

222 tide substrates from one another, leading to native protein refolding.

223 Electrospray ionization (ESI) of native proteins results in a narrow distribution of low

224 RBDmap thus yields profound insights into native protein-RNA interactions in living cells.

225 that the beta-barrel is responsible for the native protein's amphiphilicity.

226 -sfAFP, the native protein, and D-sfAFP, the native protein's enantiomer).

227 ide strategy, we designed a peptide with non-native protein sequence, AP3, which exhibited potent ant

228 ave not been performed in the context of the native protein sequence.

229 tackle these questions, we in silico mutated native protein sequences into random sequence-like ensem

230 nexplored, one might speculate that reversed native protein sequences should be significantly more fo

231 s revealed important differences between the native protein shell and the empty capsid.

232 the degree of denaturation, compared to the native protein solution, was observed.

233 oncentrations of potentially aggregating non-native protein species within the cell.

234 es indicates that the characteristics of the native protein structural fluctuations that cause proton

235 s been made in designing proteins that mimic native proteins structurally, it is more difficult to de

236 where Zn(2+) is crucial for maintaining the native protein structure but the Zn-bound cysteines are

237 Computationally generated non-native protein structure conformations (or decoys) are o

238 Access to native protein structure depends on precise polypeptide

239 Voids and cavities in the native protein structure determine the pressure unfoldin

240 ectrostatic separation process preserves the native protein structure found in NBF and improves the r

241 or these cationic residues in the context of native protein structure where histidine protonation sat

242 ein folding, unlike the formation of stable, native protein structure, does not present a substantial

243 the three modifications, would be buried in native protein structure.

244 ely to lead to artifactual conclusions about native protein structure.

245 to identify those models most similar to the native protein structure?

246 protein folding pathways leading to knotted native protein structures are slower and less efficient

247 n blind test of a computer method to predict native protein structures based solely on an all-atom ph

248 knowledge-based potentials in selecting the native protein structures from decoy sets.

249 A set of native protein structures provides the positive training

250 Entropic stabilization of native protein structures typically relies on strategies

251 kbone hydrogen bonds are a common feature of native protein structures, yet their thermodynamic and k

252 importance of side-chain packing in forming native protein structures.

253 made it especially suitable for the study of native protein structures.

254 produced are often more compact and reflect native protein subcomplexes when compared with unmodifie

255 HslU hexamers recognize and unfold native protein substrates and then translocate the polyp

256 e ATP-dependent unfolding and degradation of native protein substrates in conjunction with ClpP1P2, b

257 emonstrate that although AtPARK13 can act on native protein substrates, unfolded proteins represent b

258 ea, which are known to stabilize or denature native proteins, suggesting that interface-driven Abeta

259 n common with unfractioned SEA, the purified native protein suppresses lipopolysaccharide-induced DC

260 Designing proteins or peptides that bind native protein targets can aid the development of novel

261 ing of a secondary structural element of the native protein, termed a "foldon." Each folded segment i

262 d to clearly defined structural units in the native protein, termed the blue and red foldons.

263 t two other distinct structural units in the native protein, termed the green and yellow foldons.

264 By connecting the native protein termini via a covalent linker and introdu

265 which displays the defining features of the native protein that have not been attainable from struct

266 es result in distinct binding modes with non-native proteins that ultimately define the activity of t

267 As a programmable tool orthogonal to any native proteins, the DNA-lipid tethers can be further ap

268 Unfortunately, the native proteins themselves are often unstable in physiol

269 both termini, suggest their proximity in the native protein; these are also disordered, based on POND

270 g, hyaluronan-independent association of the native protein to brain subcellular membranes.

271 antibodies to rRH4(30) block binding of the native protein to erythrocytes, these antibodies failed

272 lenges associated with the administration of native proteins to C. elegans have limited the range of

273 that LSs can be chemically cleaned to remove native proteins to create intact clean hollow LS shells.

274 eta-sheet-rich structures in transition from native proteins to ordered oligomers and fibres.

275 released to a cf-IMER column which converted native proteins to peptides in 5 min at elevated tempera

276 plicit solvent were performed on a set of 75 native proteins to test the various energy potentials.

277 ion could provide a general means to deliver native proteins to the cytosol.

278 haperone Hsc70 assists in the folding of non-native proteins together with its J domain- and BAG doma

279 ated protein treated mice as compared to its native protein treatment.

280 raffic to the plasma membrane, and place the native protein under optical control provided by the coa

281 -based AFM to the multiparametric imaging of native proteins under physiological conditions.

282 During amyloid formation, the native protein undergoes a tetramer-to-folded monomer tr

283 here a scheme for optical remote control of native proteins using a "photoswitchable conditional sub

284 modest resolution (2.8 to 2.3 angstroms) for native proteins varying in size (127 to 1148 unique resi

285 tein gave a single band on SDS-PAGE, and the native protein was a homodimer of 32-kDa monomers.

286 The molecular weight of the native protein was estimated at approximately 59,400, a

287 fied recombinant MATN-1, along with purified native protein, was shown to inhibit angiogenesis in viv

288 sidues within the inter-domain linker in the native protein, we were able to show that despite the re

289 tart of gel photopatterning to completion of native protein western blotting, a substantial time savi

290 For both recombinant and native proteins, western analysis detected MERTK interac

291 tant in the MBP sequence but adjacent in the native protein where they close the longest residue-to-r

292 t AML-1b showed the same band pattern as the native protein, whereas recombinant AML-1a in the reduce

293 hanced neuronal expression compared with the native protein while maintaining transcriptional activat

294 (DMG) intermediates, an expanded form of the native protein with a dry core, have been observed durin

295 tion produced major reduction in recovery of native protein with both systems, the result of strongly

296 al route to generating synthetic variants of native proteins with more selective binding profiles.

297 In contrast, native proteins with more uniform surfaces are destabili

298 l signal peptides mediate the interaction of native proteins with the translocon complex of the endop

299 n degradation machinery to remove unmodified native proteins within minutes of application.

300 A molecule page starts with astate of a native protein, without any modification and/or interact