ライフサイエンスコーパス: native state

1 hanged to its NIS-bound state instead of the native state.

2 ective lens to image frozen samples in their native state.

3 olded state and PP2 helical structure in the native state.

4 as able to significantly protect the protein native state.

5 guide it along an ordered pathway toward the native state.

6 protein into a topology close to that of its native state.

7 nsions of an intact vertebrate in a close-to-native state.

8 ation that is topologically simpler than the native state.

9 ormations, slowing productive folding to the native state.

10 preformed amyloidogenic beta-strands in the native state.

11 characterize the P13 protein complex in its native state.

12 e the cell over the elongated aqueous buffer native state.

13 tructure and active-site cleft formed in the native state.

14 ong different parallel tracks leading to the native state.

15 setting, with high precision, and in a near-native state.

16 hich leads to the spontaneous folding of the native state.

17 le proteins are strongly funneled toward the native state.

18 ps to maintain proteins in their functional, native state.

19 of the population for 1.4 ms en route to the native state.

20 slower 1.4-ms phase during refolding to the native state.

21 unique three-dimensional structure in their native state.

22 energy landscape and fold efficiently to the native state.

23 lecular conformations that exchange with the native state.

24 IkappaBalpha AR domain was unfolding in its native state.

25 t adopt a stable tertiary structure in their native state.

26 in directing the folding process toward the native state.

27 ise consolidation that ultimately yields the native state.

28 ding energy landscape more biased toward the native state.

29 no known propensity for instability in their native state.

30 s that correspond to the four helices in the native state.

31 ay influence the capacity to fold toward the native state.

32 es, whereas natural selection determines the native state.

33 have a small effect on the stability of the native state.

34 ike particles, in three dimensions in a near-native state.

35 ultimately form helices I, II and IV in the native state.

36 ulate multiple routes on this journey to the native state.

37 ive structures and guides the search for the native state.

38 the foundation for productive folding to the native state.

39 st misfolded substrate proteins to reach the native state.

40 save the competing folding transition to the native state.

41 etics to both the intermediate state and the native state.

42 intermediate that transitions slowly to the native state.

43 plicated, involving multiple channels to the native state.

44 r for Ags that are poorly processed in their native state.

45 te and complex equilibrium dynamics near the native state.

46 decreasing the probability of occupying its native state.

47 regates and assist in their refolding to the native state.

48 progressively funneling the molecule to the native state.

49 that the intermediate strongly resembles the native state.

50 ide the context for further folding into the native state.

51 es for each to propagate to the final folded native state.

52 y MG and forces the nascent chain toward the native state.

53 f freedom and the overall flexibility of the native state.

54 gements, making it difficult to study in its native state.

55 ions with chaperones before folding into its native state.

56 bel-free, biomolecular fingerprinting in the native state.

57 the protein along productive pathways to the native state.

58 cs rather than binding preferentially to the native state.

59 t able to support part of the search for the native state.

60 adopted as an alternative to the functional, native state.

61 the entire energy landscape and not just the native state.

62 echnique for analyzing biomolecules in their native state.

63 ions that occur in unfolded RNAs relative to native states.

64 is of unperturbed cells and tissues in their native states.

65 -atom simulations for both the denatured and native states.

66 at about five sites to let them attain their native states.

67 gical samples of complex topography in their native states.

68 sity maps of biological specimen in its near native states.

69 ue dissociation and preserves transcripts in native states.

70 ures of macromolecular complexes in close to native states.

71 be an important tool for describing protein native states.

72 parallel pathways between the denatured and native states.

73 d in the hydrophobic cores of the respective native states.

74 solution structures of the proteins in their native states.

75 e use Markov models to determine whether the native state acts as a facilitator for the transition, o

76 4 of the 12 molecules, we conclude that the native state acts primarily as a trap, which is consiste

77 = 250 ns, the fraction of structures in the native-state (alpha-helical) basin equals 22%, which is

78 In the meantime, the native state also becomes more unstable and the amyloid

79 ants are rapidly committed to folding to the native state, although they reach the native state with

80 Native state analyses led to the discovery that the disu

81 nges between a highly populated, NMR-visible native state and a conformationally excited, NMR-invisib

82 the overall interconversion rate between the native state and a well-defined folding intermediate by

83 field-free analysis of biomolecules in their native state and at or near physiological salt concentra

84 of the target protein while maintaining its native state and biological activity.

85 e for the full-length glycoprotein in a near-native state and compared it to that of a glycoprotein l

86 The correlation between energy of the native state and experimental denaturation temperature w

87 myosin 2 have the same structure in both the native state and following specific, intramolecular phot

88 sualization of cellular organization at near-native state and in sub-molecular resolution, making it

89 bed in terms of transitions between a folded native state and less-populated intermediates, or excite

90 how that the denatured RfaH refolds into the native state and that RfaH in which the order of the dom

91 the ratio of the free energy gap between the native state and the average of non-native states versus

92 able SP, we show that as the SP folds to the native state and the population of unfolded SP declines,

93 t is, the free energy difference between the native state and the unfolding transition state-dictates

94 ner, i.e. by lowering the free energy of the native state and with almost no effect on the free energ

95 entails attractive forces for the functional native states and discrimination against potential nonna

96 h atomic resolution 3D structures of protein native states and some folding intermediates are availab

97 commercial gasoline mixtures, both in their native states and spiked with different adulterants.

98 onal domains to the monomer destabilizes the native state, and the entropically stabilized amyloid-li

99 are investigated both as-prepared, in their native state, and under reaction conditions, while trans

100 en the metastable mirror-image state and the native state; and (iv) offer a plausible reason to expla

101 In native states, animal cells of many types are supported

102 In contrast, myristoyl interactions in the native state are highly specific and tuned for sensitive

103 her residues experiencing frustration in the native state are in structured regions of the EIS.

104 tration, wherein not all interactions in the native state are optimized energetically, thereby potent

105 transition states from both I1 and I2 to the native state are plastic and change with mutation and de

106 show that residues forming helix III in the native state are unstructured in the EIS while other res

107 ely in an all-or-none fashion and thus their native states are well protected from thermal fluctuatio

108 foreshadowed by segmental flexibility in the native state as probed by heteronuclear NMR spectroscopy

109 cting the protein folding into a compact non-native state, as well as protein interactions with the l

110 ES is required by a set of proteins to reach native state at a biologically relevant timescale, avoid

111 three-dimensional images of cells in a near-native state at macromolecular resolution, but identifyi

112 al and computational methods to describe non-native states at atomic resolution.

113 and suggests that the general concept of the native state be expanded to allow for more variable phys

114 at both proteins exist as tetramers in their native state before and after proteolysis.

115 Thus, P3 is disrupted during folding to the native state but not to the misfolded state, and P3 disr

116 dictated not only by factors stabilizing the native state, but also by potentially independent factor

117 rd, promoting normal skin homeostasis in the native state, but can be metabolized to sphingosine-1-ph

118 e energetically disfavored compared with the native state, but entropically favored.

119 same glycans can be identified also in their native state by CE-MS without derivatization.

120 in three different proteins stabilizes their native states by -0.7 to -2.0 kilocalories per mole and

121 hods to probe the adhesion of cells in their native state can greatly advance the understanding of co

122 , discuss how local backbone disorder in the native-state can lead to non-classical Phi(M)-values (Ph

123 on of the Xi topological arrangement in near-native state cells.

124 ed state of HEWL possesses a large degree of native state characteristics.

125 c dynamics, encoded by their structure under native state conditions.

126 We propose that the native state contains structural elements that frustrate

127 cells under two different conditions, i.e., native state (control) and latrunculin B (treated), we s

128 we demonstrate that stability of the protein native state determines both protein aggregation propens

129 nalysis of the xanthophyll fraction in their native state (direct extract) revealed that most of them

130 e productive structural rearrangement to the native state does not occur in a single swift step but i

131 Steric clashes in the native state due to the new side chain are rarely observ

132 ions to the free energies of the TSE and the native state during folding provide insights into struct

133 ns is an important factor in determining the native-state dynamical properties of human lysozyme in t

134 plete picture of the energy landscape of the native state dynamics of an AR domain.

135 olecular dynamics simulations to compare the native state dynamics of Beta-2 microglobulin (beta2m),

136 the loop as well as its size influences the native state dynamics, which can impact receptor binding

137 ddress the effects that location may have on native-state dynamics, as studied by hydrogen-deuterium

138 utation (I23A versus I59T) is crucial to the native-state dynamics, with the alpha-domain mutation ha

139 One mutation destabilizes native-state electrostatic interactions but has a larger

140 ated from within a thermodynamically stable, native-state ensemble.

141 This remarkable native-state expansion can be explained quantitatively b

142 ly quenched by surrounding nitroxides in the native state; exposure to ascorbate or ascorbate/glutath

143 city of the equilibrium folding, flux to the native state flows through multiple channels and can be

144 ather than for the overall unfolded-state to native-state folding pathway.

145 y gated ET, which involves conversion to the native state followed by reduction, with a small fractio

146 The dominance of a single native state for most proteins under ambient conditions

147 action and of the ability to protect the TTR native state for polyphenols, both stilbenoids and flavo

148 We show that both hGAAP and vGAAP in their native states form oligomers and that oligomerization is

149 a(6-85) takes only microseconds to reach its native state from an extensively unfolded state, while t

150 However, the protein has an unusually high native-state heat capacity, and consequently, the change

151 egments that form secondary structure in the native state, helix(1) shows a decreasing trend of oxida

152 Native-state hydrogen exchange (NSHX) has been a powerfu

153 ence of (1)HN and (15)N chemical shifts, and native-state hydrogen exchange at urea concentrations wh

154 Moreover, native-state hydrogen exchange indicates that Hsp90 can

155 The native-state hydrogen-exchange data suggest that the six

156 regions of proteins and in proteins in a non-native state (i.e. misfolded or unfolded states).

157 t is essential to keep the cells under their native states (i.e. label-free), establishing plasmonic

158 tions, this intermediate either folds to the native state in a few minutes, or forms amyloid fibers i

159 released intermediates to slowly reach their native state in solution.

160 PGK has a more compact native state in the cellular compartments than in aqueou

161 imum WUE(e) across all biomes; and a minimum native state in wetter years that was common across hydr

162 responding to the denatured, transition, and native states in the folding of the activation domain of

163 chemical analysis on target samples in their native states in the undisturbed environment.

164 he barrier separating the molten globule and native states increases, although the barrier to the amy

165 ithout compromising folding cooperativity or native state integrity.

166 , whereas the third introduces unanticipated native-state interactions but does not alter electrostat

167 - or three-state mechanisms involving mainly native-state interactions, yet recent network models bui

168 lding trajectory, the times for reaching the native state, interactions stabilizing all the substruct

169 ons in gpW at 1 degrees C, we found that the native state interconverts with a transiently formed, sp

170 DESI enabled the direct, rapid, native state interrogation of oil samples on steel surfa

171 n of peptides or proteins from their soluble native states into intractable amyloid deposits is assoc

172 hod might be applicable to proteins in their native state, irrespective of location, concentration, a

173 age of trajectories that are mediated by the native state is 31% when averaged over all molecules, an

174 he transition from the unfolded state to the native state is entropically disfavored at 298 K.

175 that restoring degraded ecosystems to their native state is generally superior to agricultural produ

176 cytoskeletal functions in the cytoskeleton's native state is inherently difficult due to its rigid an

177 under thermodynamic conditions in which the native state is no longer stable, such as denaturing sol

178 ant FF domain has been designed in which the native state is selectively destabilized by removing the

179 ve and repulsive exosite interactions in the native state is shifted to favor the attractive interact

180 Mass analysis of the intact protein in its native state is straightforward and fast, requires very

181 of this species is often difficult since the native state is usually predominantly populated at equil

182 NIR-NFP, which is optically silent in its native state, is composed of multiple self-assembled pep

183 HP24stab shift the energy balance toward the native state, leading to a minimal folding motif.

184 the orthogonal fragment screening methods of native state mass spectrometry and surface plasmon reson

185 t there is a tremendous opportunity to apply native state mass spectrometry as a complementary fragme

186 an underutilized fragment screening method, native state mass spectrometry, together with two proven

187 eds along routes that are different from the native state mechanism explored here.

188 The synchronization of native state motions as they transition between microsta

189 In the native state, mu1 determines the environmental stability

190 reveals that loops and alpha-helices in the native state must undergo conformational transitions to

191 tions in vitro, a small fraction reaches the native state (N) with kobs approximately 0.6 min(-1), wh

192 t is proposed to differ in topology from the native state (N).

193 ng the transition state (TS) relative to the native state (N).

194 Apart from the native state, native-like and molten-globule states have

195 les stabilizes globular proteins because the native state occupies less space than the denatured stat

196 It has been suggested that the native state of a protein acts as a kinetic hub that can

197 The native state of alpha-synuclein was probed by gel filtra

198 Because the native state of BBL is sensitive to experimental conditi

199 icroscopy studies are unable to preserve the native state of chemically reactive and beam-sensitive b

200 molecular mechanisms of oligomerization, the native state of GAAPs in living cells and the functional

201 ermodynamic and structural properties of the native state of HisF.

202 ccessible conformers in equilibrium with the native state of immature SOD1 (apoSOD1(2SH)).

203 d out of cells, perturbing the autoinhibited native state of Parkin, resulting in an active-for-autou

204 d hydrodynamic techniques to investigate the native state of PCM components in the cytoplasm.

205 ter extraction method appeared to retain the native state of pectin molecules compared with acid and

206 h crowding being responsible for the compact native state of PGK in the cell.

207 eciphering the mechanisms that stabilize the native state of proteins, as well as those that maintain

208 consider flexibility of each residue in the native state of proteins.

209 ed mutation (F198S) known to destabilize the native state of PrP was also found to stabilize the beta

210 wever, under conditions that favor the fully native state of Sso AcP, such an interaction is not esta

211 This property may confer on the native state of such proteins significant elasticity.

212 ts that the osmolyte glycerol stabilizes the native state of the human cardiac I27 titin module again

213 It appears, therefore, that the native state of the prion protein undergoes substantial

214 a transition state that is distinct from the native state of the protein.

215 various paramagnetic species present in the native state of these samples and understand their molec

216 The native state of this 35-residue polypeptide contains sev

217 uding natural polyphenols, which protect the native state of TTR by specifically interacting with its

218 The methods used maintained the endogenous native state of tumours and we exploited this to investi

219 The functional native states of globular proteins become unstable at lo

220 Unfolded states of proteins and native states of intrinsically disordered proteins (IDPs

221 we probe the conformational landscape of non-native states of lysozyme containing a single native dis

222 shifts about conformational fluctuations in native states of proteins.

223 tive molecular dynamics investigation of the native states of wild-type and Z alpha1-AT, revealing a

224 exhibit CCS values similar to those of the "native-state" of the protein.

225 MBP then folds to the native state on a longer time scale (~100 s), suggestive

226 n of a random coil unfolded state toward the native state on an energy surface rough with local minim

227 cedures often alter the molecules from their native state on the cell; 2) Long processing time before

228 ns exhibit levels of order comparable to the native state on the nanosecond time scale.

229 denaturant and is in slow exchange with the native state on the nuclear magnetic resonance time scal

230 an essential lysine can be expressed in its native state only upon genetic incorporation of N--acety

231 ne to aggregation are thought to reach their native state optimally.

232 olume excluded by the crowder favors compact native states over expanded denatured states, enhancing

233 -ray crystallography, NMR, dynamics studies, native state pKa measurements, and thermodynamic analysi

234 MPC in its native state possess very poor solubility, therefore, su

235 with mutations that, destabilizing proteins native state, promote their aggregation.

236 functions can also describe fluctuations in native-state protein ensembles.

237 acquisition of distinct determinants of the native state, provides unprecedented evidence for a sequ

238 With the native state, putrescine2+ is a weak competitor when the

239 they increase the fraction that reaches the native state rather than forming the misfolded state.

240 We conclude that Ltn native-state rearrangement proceeds by way of a large-sc

241 tiary interactions and the attainment of the native state relative to the free protein.

242 ctra from biogenic guanine crystals in their native state, resolving their characteristic C-H, N-H an

243 ional reconstructions of whole cells in near-native states revealed a thin PG-like layer extending fr

244 tools to obtain receptor complexes in a near-native state, revealing insights into the gross conforma

245 deduced a free-energy surface that exhibits native-state ruggedness with apparently similar barrier

246 ssist proper folding, Cdk1-cyclin B1 acts on native state separase.

247 Mass spectrometry acquired in the native state showed that the onset of wild-type beta2m o

248 In the non-native state (significantly explored at 380 K), the prob

249 show that when variants have similar reduced native-state stabilities, the location of the mutation (

250 consistent with the P14 mutations perturbing native state stability and shifting an allosteric equili

251 The correlation between the native-state stability and compactness of the denatured

252 idate our approach by demonstrating that the native-state stability as a function of pH is accurately

253 fect of a mutation that has minor effects on native-state stability at the domain interface (I56V) an

254 utation results in only minor changes to the native-state stability of lysozyme.

255 isfolding intermediates is an alternative to native state stabilization and requires substoichiometri

256 Native state stabilizers are promising drugs to treat TT

257 the reactive center loop P14 residue from a native state stabilizing interaction with the hydrophobi

258 ach it is possible to fold proteins to their native states starting from extended structures.

259 of the C-terminal helix, which may alter the native-state structural ensemble of the molecule, leadin

260 mic-scale insight into the attributes of the native state that encodes the broad specificity, we used

261 keleton and its associated proteins in their native state that is also compatible with current mass s

262 ly high barrier separating the denatured and native states that folding appears cooperative even unde

263 s of the model alpha/beta protein VA3 in its native state, the coupling between the main-chain (MC) m

264 In its native state, the last 56 amino acids of the ectodomain

265 ss in which the intermediate converts to the native state through a downhill barrierless transition.

266 show that Gbeta interacts with CCT in a near-native state through interactions of the Ggamma-binding

267 e funneled landscape, proteins fold to their native states through a stochastic process in which the

268 hysical agents can guide Env from the stable native state to perturbation-sensitive forms and modulat

269 It populated the same native state, transition state, and folding intermediate

270 HSD2 from pH 7.4 to 4.5, possibly due to the native state unfolding to the intermediate ion core stat

271 allosteric mechanism of Par-6 suggests that native-state unfolding may be essential for the function

272 ers in water were directly observed in their native state using cryo-TEM measurements.

273 ediate, and selective destabilization of the native state using point mutations.

274 temperature near equilibrium dynamics of the native state, using phosphoglycerate kinase as model pro

275 ween the native state and the average of non-native states versus the roughness measured by the varia

276 roEL-MSG complex led to the formation of the native state via a compact intermediate with the rate se

277 forms a "prepore" structure that attains the native state via a conformational switch.

278 These native state volume fluctuations were found to correlate

279 This enhancement in the non-native state was due to glycine, as demonstrated by simu

280 ainst exchange with solvent deuterium in the native state was found in a subset of buried positions i

281 5 gene chromatin, isolated from yeast in the native state, was remodeled by yeast extract in a gene a

282 of one of these misregistered states to the native state were captured in three independent simulati

283 the rate of folding and the structure of the native state were in good agreement with experiments.

284 mation on how purified proteins attain their native state when refolded in the test tube, starting fr

285 A sluggish peroxidase in its native state, when bound to CL, cyt c catalyzes CL perox

286 ly half of the ribozyme folds readily to the native state, whereas the other half forms an intermedia

287 of the band that corresponds to loops in the native state, whereas the rest of the regular elements o

288 re as unfolding proceeds, in contrast to the native state, which displays a temperature-independent d

289 s a well-defined free energy well around the native state, which makes the interaction stable.

290 We also characterize a near-native state with a highly mobile transmembrane helix 5

291 substitution of the Met80 heme ligand of the native state with a lysine ligand from a surface Omega-l

292 to the native state, although they reach the native state with approximately the same rate constant a

293 utant TTR suggest a structure similar to the native state with only a simple opening of a beta-strand

294 It compares the energy distributions of the native state with respect to structural decoys.

295 d imaging of erythrocyte morphology in their native states with a nm path length sensitivity.

296 details of the primary cell wall in its near-native state, with implications for microfibrils motions

297 al environment and less on properties of the native state, with the exception of alpha-helical residu

298 (ABPP) of propofol-binding proteins in their native state within mouse synaptosomes.

299 nsional structures of the chloroplast in its native state within the cell.

300 otted, denatured polypeptide to the knotted, native state without the aid of chaperones.