ライフサイエンスコーパス: nativelike

1 rotection; hence, the mutant complex appears nativelike.

2 duced core module conformations samples both nativelike 4:4 and non-native 3:5 beta-hairpin structure

3 ants containing these disulfides demonstrate nativelike absorption spectra and light-dependent activa

4 ate that persistent hydrophobic clusters are nativelike and may account for the ability of ligand to

5 t the transition state of bound MDM2 is more nativelike and more heterogeneous than that of apo-MDM2.

6 y creating a free energy gap between sets of nativelike and nonnative conformations.

7 This shows that holo-CaM-F is less nativelike and the EF hand "closes down" about the fluor

8 e that this is due to an equilibrium between nativelike and unfolded molecules rather than formation

9 sition state between I2 and N2 is relatively nativelike, as determined by the sensitivity of the rate

10 Each exhibits a nativelike B chain and less-ordered A chain.

11 The nativelike basin is fractionally populated (DeltaG(300K)

12 mational plasticity, Delta98Delta exhibits a nativelike beta-barrel topology and is able to support a

13 ermediate state which slowly rearranges to a nativelike beta-clamshell structure.

14 ch was previously suggested to form a stable nativelike beta-strand), with the adjacent segments exhi

15 sidence time of the ligand in the native and nativelike binding poses is distinctly longer than that

16 ue approach to distinguishing the native (or nativelike) binding pose from decoy poses that cannot be

17 Still, the fragment retains nativelike carbohydrate binding properties.

18 in the transmembrane segments, resulting in nativelike complexes that can be interrogated by MS.

19 erved under visible light can be reversed to nativelike conditions upon exposure to UV light, leading

20 from solutions in which the molecules have a nativelike conformation and is particularly advantageous

21 ducing agent results in the acquisition of a nativelike conformation for the reduced protein.

22 The beta subunit adopts a nativelike conformation in Zwittergent 3:12 micelles as

23 weight clusters and aggregates that retain a nativelike conformation within the monomers.

24 ofactor is not required for refolding into a nativelike conformation, does not remain associated with

25 ormational sampling or "soft refolding" to a nativelike conformation.

26 The acid-denatured state populates nativelike conformations with both alpha-helical and bet

27 lizing integral membrane proteins in stable, nativelike conformations.

28 ure, and that by incorporating a cross-link, nativelike core structure will dominate the ensemble as

29 The variant side chain remains buried in a nativelike crevice with small adjustments in surrounding

30 u(II) binding causes beta2m to organize into nativelike dimers and tetramers that precede amyloid for

31 s at position 73 and 91 are included and the nativelike disulfide bond is formed.

32 (FKKGERL) from the src SH3 domain adopts the nativelike diverging type II beta-turn in aqueous soluti

33 Nativelike elements of structure are retained in the B c

34 alpha-helices, relative to that seen in the nativelike environments.

35 DAGK stock solutions (where DAGK maintains a nativelike fold and oligomeric state) and lowest in reac

36 absence of P5abc, the intron RNA maintains a nativelike fold but its active-site helices are not tigh

37 As further support for a nativelike fold in Ca(2+), ribozyme that has been prefol

38 ozyme-substrate complex becomes trapped in a nativelike fold preceding the chemical transition state.

39 plified model system, called BLBC, retains a nativelike fold.

40 drive the entire domain to acquire a stable, nativelike fold.

41 de quenching of Trp fluorescence supported a nativelike folding conformation.

42 ents or represents the rate of escape from a nativelike folding trap.

43 create statistically significant (p < 0.01) nativelike folds for RNAs of known structure ranging fro

44 es that the denatured state of eglin C has a nativelike global structure, a conclusion reached indire

45 tween pH 7.0 and pH 4.0, apoLp-III retains a nativelike helix bundle structure.

46 rewlike rotation motion during the export of nativelike helix-turn-helix conformations.

47 t local electrostatic fields which result in nativelike heme chromophore properties (spectroscopy, el

48 e-chain hydroxyl group appears to maintain a nativelike hydrogen bond with D47 at pDcorr 12, even tho

49 Thus, a template of nativelike hydrophobic contacts in the unfolded state ma

50 the polypeptide chains in the 4-OT dimer are nativelike in structure with the exception of their C-te

51 predominant early unfolding intermediate was nativelike in structure, in agreement with previous NMR

52 Residues of pKID directly involved in nativelike interactions at the interface were found to s

53 The conformational folding of the nativelike intermediate des-[40-95] on the major oxidati

54 5 showed that this variant unfolds through a nativelike intermediate that has properties consistent w

55 A relatively nativelike intermediate, I(N), was observed between pH 4

56 ptide samples three main free energy basins (nativelike, intermediate, and unfolded) separated by sma

57 These mutants are analogues of two nativelike intermediates, des[40-95] and des[65-72], who

58 , and seven-disulfide isomers, including two nativelike isomers, SLPI-6A and SLPI-7A, as transient in

59 eptide coaggregates rather than well-defined nativelike LH complexes.

60 technology provides membrane proteins with a nativelike lipid bilayer and much-needed solubility and

61 able, and monodisperse model membrane with a nativelike lipid bilayer.

62 ed for the study of membrane proteins in the nativelike lipid environment.

63 monodisperse, stable model membranes with a "nativelike" lipid bilayer.

64 Its nativelike pK is indicative of nativelike local electrostatics, and consistent with Fe2

65 A nativelike low-resolution structure has been shown to pe

66 omplex while maintaining a conformationally "nativelike" membrane protein structure in the gas phase.

67 Nativelike modulation of channel properties was observed

68 ble one- and two-disulfide isomers, only two nativelike one-disulfide isomers, BoINF-alpha (Cys1-Cys9

69 The distorted DNA retains nativelike pairing of bases at ambient temperatures, but

70 Its nativelike pK is indicative of nativelike local electros

71 the titration of histidines with depressed, nativelike pK(a) values in the denatured state (D).

72 designed heterodimeric coiled coil with the nativelike properties of sigmoidal thermal and urea-indu

73 d either or both 264 and 475 did not restore nativelike properties to the Oriental variant.

74 oduced a recombinantly expressed enzyme with nativelike properties; in contrast, mutating arginine 47

75 The data suggest retention of native or nativelike protein conformations using the air amplifier

76 Although variant T- and R-protomers retain nativelike protein surfaces, the receptor-binding activi

77 cur in the chaperonin cavity with release of nativelike proteins into the bulk solution.

78 structurally heterogeneous and still retain nativelike residual structures.

79 lin mutants were found to retain substantial nativelike secondary and tertiary structure under all co

80 t structures with appreciable populations of nativelike secondary structural elements.

81 rium intermediate that has approximately 90% nativelike secondary structure and significant enthalpic

82 peptide fragments that can achieve defined, nativelike secondary structure is presented.

83 The mutants had a nativelike secondary structure similar to that of wild t

84 of WT-ILBP with an intermediate that showed nativelike secondary structure, whereas C69F-ILBP follow

85 splays an intermediate during unfolding with nativelike secondary structure.

86 ndergo local hydrophobic collapse and sample nativelike secondary structure.

87 source-activated transthyretin tetramer and nativelike serum amyloid P decamer, were separated in io

88 y a parent alpha-peptide sequence as well as nativelike side-chain display in the vicinity of the bet

89 Therefore, nativelike side-chain interactions between these residue

90 structures intermediates, which may yield a nativelike signal in the fluorescence measurements typic

91 events which begin with stabilization of the nativelike, slow exchange core.

92 ombinant protein resulted in a protein with "nativelike" spectroscopic properties and homogeneous CO

93 The presence of NAD restored nativelike stability to the mutant.

94 When this form was converted back to a nativelike state, concomitant loss of recognition by GT

95 es indicate that lysozyme transitions from a nativelike structure at low surfactant concentration to

96 se resulted in a fully assembled enzyme with nativelike structure but lacking catalytic activity.

97 that target protein binding can restore the nativelike structure critical to function, emphasizing t

98 ions within the 58-72 segment that lead to a nativelike structure even in its fully reduced form.

99 ucture, and that the oxidized module samples nativelike structure for a greater fraction of the time

100 ted distance dependence is consistent with a nativelike structure for the exchange-competent conforma

101 Thus, nativelike structure in the denatured protein is stabili

102 uctural change, since the ribozyme assumes a nativelike structure when folded in the presence of Ca(2

103 the cyanomet isoform of the permutein show a nativelike structure with a heme binding pocket very sim

104 s required for the domain to adopt a stable, nativelike structure, 3 proteins of different lengths we

105 in a single polypeptide molecule, will favor nativelike structure, and that by incorporating a cross-

106 for the ability of ligand to bind and induce nativelike structure, even at pH 2.3.

107 oupled polymer surfactant molecules, exhibit nativelike structure, function, and backbone dynamics ov

108 exposure, however, the protein refolds to a nativelike structure.

109 ation or dissolution: when the peptide is in nativelike structures and one of the distances shortens

110 DSC profiles of Nkx2.5 indicate fluctuating nativelike structures at <37 degrees C.

111 eptameric structure in solution and exhibits nativelike substrate-refolding activity.

112 of compact conformations are far from being nativelike, suggesting that the search for the folded st

113 o the native spectrum; however, considerable nativelike tertiary contacts remain.

114 hese results suggest that a set of native or nativelike tertiary interactions, distributed throughout

115 The nativelike tertiary structure of the monomer suggests a

116 ues suggest that the stable helices are more nativelike than other regions in both bound MDM2(N) and

117 able helices, helix II in bound MDM2 is more nativelike than that in apo-MDM2.

118 Calpha distance matrix is significantly more nativelike than the Calpha-Calpha matrices corresponding

119 wever, helix I and IV in bound MDM2 are less nativelike than those in apo-MDM2.

120 ed single modules from folding properly to a nativelike three-dimensional structure.

121 ough two separate pathways in which separate nativelike three-disulfide species are populated.

122 p in both pathways involves formation of the nativelike three-disulfide species, a step in which most

123 eometric simulations speed up the search for nativelike topologies as there are no energy barriers to

124 Stable nativelike topologies that persist on unfolding would ex

125 observed at the higher temperatures possess nativelike topology and overall conformation, with many

126 lding process, the protein backbone adopts a nativelike topology while certain secondary structure el

127 on of the profilin-G-actin complex involve a nativelike unfolding intermediate of profilin.

128 We have discussed the relative importance of nativelike versus nonnative tertiary contacts for the fo