ライフサイエンスコーパス: natively

1 Neither made IL-1ra natively.

2 This method natively accounts and corrects for background photons an

3 Natively, acid resistance in Escherichia coli is largely

4 Misfolding of the natively alpha-helical prion protein into a beta-sheet r

5 ere, on the contrary, evidence for an almost natively alpha-helix-rich monomeric precursor state with

6 epithelial fusion failure 1 (EFF-1) protein natively anchored in cell-derived membrane vesicles.

7 In this study, we examined the coupling of natively and heterologously expressed nociceptin/orphani

8 reover, CD146(+) perivascular cells express, natively and in culture, molecular markers of the vascul

9 dence that the conformational composition of natively and nonnatively unfolded proteins fail to appro

10 experiment, and for structure prediction of natively and nonnatively unfolded states.

11 that the NTD is partially ordered when it is natively attached to the Tudor domain.

12 that contain natural anionic domains or that natively bind to anionic nucleic acids.

13 ect pest Trichoplusia ni (TnCAD) that is not natively bound by wild-type Cry1Ac.

14 phosphatase (AP) in the presence of the two natively bound metals Zn2+ and Mg2+ produces two protein

15 rabinose transport protein and is controlled natively by an arabinose-inducible promoter.

16 ng that chaperones are able to sequester non-natively contacting residues.

17 PS was pre-equilibrated in natively contaminated sediment.

18 n analysis of a villin-type headpiece domain natively devoid of F-actin binding activity, that of sup

19 uclear magnetic resonance (SSNMR) studies of natively diamagnetic uniformly (13)C,(15)N-enriched prot

20 To explore image compression, 40 natively digital fundus images were selected with good p

21 This is striking, because rat amylin is natively disordered and not previously known to form amy

22 ematic heat capacity analysis of a family of natively disordered complementary fragments of oxidized

23 d Msh6 NTR, revealing that the Msh6 NTR is a natively disordered domain that forms an extended tether

24 henylalanine-glycine' (FG)-nucleoporins, the natively disordered domains of which line the passageway

25 We have selected a partially solvated natively disordered fragment of Escherichia coli, thiore

26 Structural data from this natively disordered gammaC peptide enhances our understa

27 This natively disordered kinetic trap, interpreted as a molte

28 The interaction of light chain LC8 with the natively disordered N-terminal domain of IC induces heli

29 sterin likely contains three long regions of natively disordered or molten globule-like structures co

30 Modeling the structure of natively disordered peptides has proved difficult due to

31 with experiments, to model the structure of natively disordered polyglutamine peptides.

32 The natively disordered protein alpha-synuclein is the prima

33 prion protein family member, Shadoo (Sho), a natively disordered protein possessing structural featur

34 We conclude that 7B2 is a natively disordered protein whose function as an antiagg

35 Natively disordered proteins belong to a unique class of

36 Many globular and natively disordered proteins can convert into amyloid fi

37 A growing number of natively disordered proteins undergo a folding/binding p

38 ls two transient helical segments flanked by natively disordered regions and a third more stable heli

39 An automatic method for recognizing natively disordered regions from amino acid sequence is

40 rn recognition algorithm to the detection of natively disordered regions in proteins.

41 ylation and OGlcNAcylation commonly occur in natively disordered regions of proteins, and often have

42 We propose that natively disordered regions with amphipathic helices for

43 nionizable residues in the growing family of natively disordered small proteins with full NMR assignm

44 alphaS, which exists in equilibrium with the natively disordered state at low [TFE] and with a highly

45 abetes, IAPP undergoes a transition from its natively disordered state to a highly ordered, all-beta-

46 have provided mechanistic insights into how natively disordered states, now commonly identified in b

47 nd activated (open) proteasomes degraded two natively disordered substrates at internal peptide bonds

48 iochemical analyses indicate that the NTR is natively disordered, and is only required for activation

49 ive region that is otherwise predicted to be natively disordered.

50 of these proteins, which are predicted to be natively disordered.

51 ts have unravelled many proteins that appear natively disordered.

52 flow cell detects the biuret complex of the natively electroinactive peptide des-Tyr-Leu-enkephalin.

53 Although long considered a natively endowed and fixed trait, working memory (WM) ab

54 he cell surface of genetically well-defined, natively Esp-deficient strains, and isogenic Esp-positiv

55 cattering in human and rat erythrocytes that natively express AQP1, in hemoglobin-free membrane vesic

56 ulated conditions in MDA-MB-231 cells, which natively express both phospholipase D1 and phospholipase

57 rin-coated vesicles in epithelial cells that natively express ENaC.

58 s are likely relevant in all cell types that natively express endogenous LRRK2, and provide insights

59 etabolism and uptake in RBL-2H3 cells, which natively express FAAH, as well as wild-type HeLa cells t

60 tern blot analysis of 3T3-L1 adipocytes that natively express FATP1 demonstrate a prominent 130-kDa s

61 In Swiss 3T3 cells, which natively express mGRP-R, two gene-specific mRNA species

62 34(+) cell-derived primary mast cells, which natively express MrgX2, responded to LL-37 for sustained

63 lock studies in rhesus monkeys, which do not natively express NFTs.

64 ptor activation in different cell lines that natively express NPY-receptors and proof the specificity

65 Using human BE(2)-C neuroblastoma cells that natively express opioid receptors and human embryonic ki

66 Human embryonic ventricular epicardial cells natively express PPARgamma, which explains the abundant

67 pression of InsP(3)R2 in RinM5F cells, which natively express predominately InsP(3)R3, increased the

68 rat vascular smooth muscle cells (VSMC) that natively express the nuclear factor of activated T-cells

69 ed human HL-60 promyeloleukemia cells, which natively express the P2Y14 receptor but had no effect in

70 re also able to observe interactions between natively expressed c-Myc and Bcr in leukemic cell lines.

71 n embryonic kidney cell line derivative, and natively expressed channel protein in a human colonic tu

72 Cofractionation of both natively expressed CNGA3 and CNGB1 (the modulatory subun

73 ter overnight treatment with PTX to suppress natively expressed Galpha subunits, voltage-dependent Ca

74 ence RNA (siRNA) was employed to silence the natively expressed Gbeta proteins in rat SG tissue and t

75 monstration of mGluR1/5 signal regulation by natively expressed Homer proteins has been elusive.

76 ologously expressed in non-neuronal cells or natively expressed in a murine neuroblastoma cell line.

77 d closing kinetics by using the Na+ channels natively expressed in GH3 cells, a clonal line of rat pi

78 essed in HEK, HeLa, and melanoma A7 cells or natively expressed in LLC-PK1 and IMCD cells, is in the

79 s provide normative data for sodium channels natively expressed in mouse muscle and illustrate the ne

80 both Piezo1 and Piezo2 channel isoforms were natively expressed in rat dorsal root ganglia tissue.

81 ary (CHO) cells, as well as the Y2 receptors natively expressed in rat forebrain, are distributed in

82 or through Kv7.4/Kv7.5 heteromeric channels natively expressed in rat mesenteric artery smooth muscl

83 To determine which Galpha proteins may be natively expressed in SCG neurons, the presence of mRNA

84 es encoding a full-length enzyme that is not natively expressed in the target bacteria.

85 an that observed in a similar mutant that is natively expressed in Thermosynechoccocus but appears to

86 Thus, sustained cholinergic stimulation of natively expressed m2/m4 muscarinic receptors controlled

87 Instead, natively expressed oleyl-acetyl-glycerol (OAG) (a struct

88 olecule is the most potent E-selectin ligand natively expressed on any human cell.

89 directly from tissue and highly enriched in natively expressed proteins exposed to the bloodstream.

90 The association can also be observed between natively expressed proteins in a panel of hematopoietic

91 co-localizes with Dbs in NIH 3T3 cells, and natively expressed Rac1 relocalizes in response to Dbs e

92 ed because tools for functionally inhibiting natively expressed RGS proteins are lacking.

93 Isolated cerebellar neurons natively expressed RGS6, GABA(B)R, and GIRK channel subu

94 First, effects on a natively expressed signalling pathway, alpha(2)-adrenerg

95 asma mass spectrometry on heterologously and natively expressed TlpD, we determined that this domain

96 for mapping the genome-wide binding sites of natively expressed transcription factors.

97 with high-throughput immunoprecipitation of natively expressed transcription factors.

98 In conclusion, in glial cells, natively expressed TRPC3s mediates a novel form of Ca 2+

99 cleotide phosphate (NAADP) strongly activate natively expressed TRPM2 channels in Jurkat T cells.

100 FFA3, whether heterologously or natively expressed, coupled via PTX-sensitive G-proteins

101 This suggests that in organisms where GR is natively expressed, GR likely contributes to stress resp

102 is showed that Gbeta1, Gbeta2 and Gbeta4 are natively expressed.

103 ected cells and also in cells where they are natively expressed.

104 the actions of CGP 12177 at recombinant and natively-expressed beta-AR.

105 we used a human mast cell-line, HMC-1, that natively expresses both receptors.

106 s NCC function in a mammalian cell line that natively expresses NCC, the mouse DCT (mDCT) cell line.

107 rated in a duodenal carcinoma cell line that natively expresses the functional hGRP-R.

108 onsistent with previous studies by others of natively expressing neurons.

109 ein-Barr virus (EBV)-transformed lymphocytes natively expressing the most common of these variants (G

110 old in human chondrocytes from an individual natively expressing the TRPV4(T89I) mutation.

111 both a T7 transcript of yeast tRNA(Phe) and natively extracted total bovine liver mt-tRNA activate P

112 Upon sample dissolution, Dox and natively fluorescent compounds were separated and detect

113 Natively fluorescent NAD(P)H are assayed directly, and n

114 l evidence for efficient CypA catalysis on a natively folded and biologically relevant protein substr

115 is system to denatured p38alpha derived from natively folded and inclusion body protein.

116 ing a conformationally diverse collection of natively folded and mutation-induced misfolded von Wille

117 ever, the sequence of reactions leading to a natively folded and oxidized protein remains unknown.

118 dination of the protein is carried out under natively folded conditions, the modification and ultimat

119 ee of frustration for a protein based on its natively folded configuration.

120 e prior in vitro results we obtained active, natively folded forms of the E. coli and Vibrio cholerae

121 ack the tightly packed tertiary structure of natively folded globular proteins.

122 induced by rgp120CM235 preferentially bound natively folded gp120 and retained strong cross-reactivi

123 In a natively folded protein of moderate or larger size, the

124 al an unprecedented mechanical behavior of a natively folded protein.

125 ges between different functional states of a natively folded protein.

126 ion, templating misfolded conformations in a natively folded protein.

127 Previous studies have shown that natively folded proteins are brittle under tension, unfo

128 sts with the mechanism of amyloidogenesis of natively folded proteins such as transthyretin and lysoz

129 These fibrils are assembled from initially natively folded proteins with an all antiparallel beta-s

130 Amyloid-forming IDPs, unlike natively folded proteins, have folding trajectories that

131 Unlike natively folded proteins, the molten globule state of ap

132 tate that is able to propagate by recruiting natively folded PrP.

133 15)N, (1)H]-TROSY correlation NMR spectra of natively folded reconstituted OmpX.

134 tion of this strategy to further stabilize a natively folded RNA conformation suggests an important e

135 We have exploited the growing database of natively folded RNA structures in the Protein Data Bank

136 Obtaining homogeneous population of natively folded RNAs is a crippling problem encountered

137 inimizing spurious interactions with stable, natively folded RNAs.

138 r nucleic acids) interact with and sequester natively folded soluble U1-70K into insoluble aggregates

139 cations for how natural proteins reach their natively folded states.

140 ceptor protein, PEB3, demonstrated that this natively folded substrate protein is not efficiently gly

141 together with its biologically relevant and natively folded substrate, the N-terminal domain of the

142 further supported by activation of PKR by a natively folded T7 transcript of tRNA(Phe)in vivo suppor

143 l states that proteins may occupy, including natively folded, disordered and amyloid.

144 he genetic code expansion concept to produce natively folded, site-specific, and lysine-acetylated Si

145 t conditions, where the monomeric protein is natively folded.

146 In contrast, edge strands that natively form beta H-bonded dimers or rings have long, r

147 variety of symmetries, most of which are not natively formed by block copolymers, including squares,

148 ar toolkit to investigate protein assemblies natively formed in the context of human disease.

149 Expression of natively glycosylated alpha dystroglycan and alpha sarco

150 Natively glycosylated and nonglycosylated recombinant fo

151 he binding affinity of the Cel7A CBM and the natively glycosylated Cel7A CBM-linker.

152 imulation to quantify cellulose binding of a natively glycosylated Family 1 CBM.

153 x vivo, both keratocan and lumican, in their natively glycosylated form, do form cross-links with col

154 The formation of a correctly folded and natively glycosylated HIV-1 viral spike is dependent on

155 tion profile, when the goal is to generate a natively glycosylated protein?

156 whereas non-cross-linked collagen I, IV, and natively glycosylated SLRPs are susceptible to degradati

157 They contain water soluble, natively highly fluorescent proteins, phycobiliproteins,

158 Fe-S covalency is much lower in natively hydrated Fd active sites than in HiPIPs but inc

159 namics of liquid water at the interface with natively hydroxylated (001) surfaces of InP and GaP phot

160 ATR dimer was found to form natively in bleached rod outer segments in vitro and whe

161 nly four eukaryotic receptors known to exist natively in both the transmembrane (TM, CD16a) and glyco

162 t regulation of TREK channels by PLD2 occurs natively in hippocampal neurons.

163 hage display for mapping vascular endothelia natively in tissue and for achieving vascular targeting

164 Eosinophils natively inhabit the small intestine, but a functional r

165 use of electromyographic (EMG) signals from natively innervated and surgically reinnervated residual

166 rst vertebrate Tc1 element described as both natively intact and functionally active, and given its r

167 Natively larger vesicles are shown to rupture earlier an

168 lved, structural information on full-length, natively membrane-anchored fusogens is scarce.

169 AC cells BIC1, in which the HPP1 promoter is natively methylated, and subjected them to 5-aza-2'-deox

170 ted in physiological relevance because cells natively migrate within complex tissue microenvironments

171 ransport was disrupted, RNA was depleted, or natively misfolded CTFs were introduced into these cells

172 quiring large amounts of highly purified and natively modified histones to be analyzed by various tec

173 We tested for such interactions in the natively monomeric human eye lens protein gammad-crystal

174 incorporating heavier atoms than those found natively; most commonly, multi- or single-wavelength ano

175 structure of a stable G-hairpin formed by a natively occurring DNA sequence.

176 The isolation and extraction of natively occurring signaling peptides (SPs) from tissue

177 ntR is freely available and can be installed natively or by using a provided pre-configured Virtual M

178 tic processes and could be identified either natively or following intensification of EGFP using immu

179 scription, we employ realistic models of the natively oxidized surfaces which go beyond the commonly

180 Although movement between natively populated alternative conformations can be crit

181 ent RNA longer than that associated with the natively positioned TEC.

182 Gluconacetobacter diazotrophicus (Gd), which natively possesses an Ala residue in the position of the

183 Salmonella enterica natively possesses both the Pdu and Eut operons, and our

184 ow, using an in-gel fluorescence assay, that natively prepared 160 and 175 kDa minimal group II intro

185 her, our results suggest that this enzyme is natively present in S. aureus cells and that it may play

186 n of the two isoforms of CX3CL1, as both are natively present in the brain.

187 ) addition, to stimulate the metabolism of a natively present microbial community.

188 Characterization of the natively processed wildtype oxidase and artificially pro

189 indole, even though S. typhimurium does not natively produce indole.

190 They can be natively produced in E. coli in very large amounts, and

191 Using natively purified proteins, we demonstrate that the pyru

192 s convert stable misfolded polypeptides into natively refolded proteins.

193 disruption of the MAL gene cluster, which is natively silent in laboratory fermentation experiments a

194 s larger than approximately 100 kDa, whereas natively smaller proteins are equidistributed.

195 alternative models include beta-helices and natively structured proteins.

196 ylated in vivo, and identification tools for natively SUMOylated proteins are rare.

197 They natively support the detection of arbitrary complex, nov

198 Here we show that natively synthesized and secreted BslA forms surface lay

199 uch a 2DEG--the SrTiO3(110)-(4 x 1) surface, natively terminated with one monolayer of tetrahedrally

200 sed by aggregation of transthyretin (TTR), a natively tetrameric protein involved in the transport of

201 ins are structurally different, varying from natively unfolded (alpha-synuclein and core histones) to

202 equilibrium between a cytosolic form that is natively unfolded and monomeric and a membrane-bound for

203 eveal that the TEAD-binding domain of YAP is natively unfolded and that TEAD binding causes localized

204 We show that both aS are natively unfolded at low temperature with different prop

205 phenylalanine-glycine motifs in filamentous, natively unfolded domains (FG domains) line the diffusio

206 has been previously proposed to exist in the natively unfolded form .

207 lpha-synuclein that ranges from a monomeric, natively unfolded form in cytosol to a physiologically f

208 a SNARE-complex chaperone over a monomeric, natively unfolded form to an amyloid-like aggregate that

209 previously unanticipated structural role for natively unfolded GLFG repeats as Velcro to link NPC sub

210 pressed primarily in peripheral neurons) are natively unfolded in monomeric form, but structured in p

211 Natively unfolded in solution, alphaS accumulates as amy

212 Alpha-synuclein is natively unfolded in solution, but can be induced to for

213 larly problematic for polypeptides which are natively unfolded in their unaggregated state, and perha

214 A pathway from the natively unfolded microtubule-associated protein Tau to

215 o in several aggregation states, including a natively unfolded monomer, a beta-sheet rich oligomer, o

216 alphaS was long thought to occur as a natively unfolded monomer, but recent work suggests it a

217 alphaS was long believed to be a natively unfolded monomer, but recent work suggests it a

218 as metastable multimers and not solely as a natively unfolded monomer.

219 Neither the natively unfolded nor the partially folded intermediate

220 We address the possible significance of natively unfolded nucleoporins in the mechanics of nucle

221 Gauging the interactions of a natively unfolded Parkinson disease-related protein, alp

222 mplex (NPC) requires nucleoporins containing natively unfolded phenylalanine-glycine (FG) domains.

223 ucleocytoplasmic transport is an assembly of natively unfolded polypeptides, which contain phenylalan

224 iction of the conformational changes in this natively unfolded protein and to the stabilization of so

225 of PD promote the in vitro conversion of the natively unfolded protein into ordered prefibrillar olig

226 is the conformational transformation of the natively unfolded protein into the aggregation-competent

227 rn can bind to and catalyze the folding of a natively unfolded protein much more readily or effective

228 hydropathy correlation for the prediction of natively unfolded protein sequences provides perhaps the

229 demonstrate in this issue of Neuron that the natively unfolded protein tau can propagate indefinitely

230 Tau is a natively unfolded protein that binds to microtubules and

231 Human Tau (hTau) is a highly soluble and natively unfolded protein that binds to microtubules wit

232 The W17F WW domain is a natively unfolded protein which adopts a native conforma

233 he manganese cluster in photosystem II, is a natively unfolded protein with a prolate ellipsoid shape

234 AlphaSyn is a natively unfolded protein with unknown function and unsp

235 A natively unfolded protein, alpha-synuclein can adopt dif

236 Alpha-synuclein, a natively unfolded protein, can adopt a number of differe

237 esent observations indicating that ActA is a natively unfolded protein, largely random coil that is r

238 As expected for a natively unfolded protein, Trp 39 was highly solvent-exp

239 n spectra of alpha-synuclein, a prototypical natively unfolded protein, were obtained in the presence

240 found that YopE had the characteristics of a natively unfolded protein, with its N-terminal 100 resid

241 of this problem is that alpha-synuclein is a natively unfolded protein, with little or no ordered str

242 locity experiments indicate that Nup2p is a "natively unfolded protein," belonging to a class of prot

243 ystem in the cell's effort to deal with this natively unfolded protein.

244 nt with the notion that alpha-synuclein is a natively unfolded protein.

245 dentifies manganese stabilizing protein as a natively unfolded protein.

246 ding diseases (Parkinson's and Alzheimer's); natively unfolded proteins (as many as 30% of eukaryotic

247 cation of Raman spectroscopy to characterize natively unfolded proteins has been underdeveloped, even

248 Although many such natively unfolded proteins have been structurally analyz

249 We demonstrate that bovine core histones are natively unfolded proteins in solutions with low ionic s

250 Natively unfolded proteins present a challenge for struc

251 nclusion that FG Nups constitute a family of natively unfolded proteins supports the hypothesis that

252 in and its homologue beta-synuclein are both natively unfolded proteins that colocalize in presynapti

253 Natively unfolded proteins, mutations that unfold protei

254 n a mapping of the free energy landscapes of natively unfolded proteins.

255 ion of the ensemble of structures present in natively unfolded proteins.

256 ensemble of secondary structures present in natively unfolded proteins.

257 lpha-synuclein and by extrapolation to other natively unfolded proteins.

258 The synucleins are natively unfolded proteins.

259 sibility in fibrin fibers is mediated by the natively unfolded regions of the molecule.

260 rather than to any changes in the monomeric natively unfolded species.

261 dulates conformational transitions between a natively unfolded state and multiple alpha-helical struc

262 rmations populated by alpha-synuclein in its natively unfolded state.

263 ther members of this family of proteins, the natively unfolded structure of manganese stabilizing pro

264 -, beta-, and gamma- synuclein show the same natively unfolded structure.

265 study demonstrates that CT is predominantly natively unfolded under physiological conditions but can

266 pha-synuclein and tau have been described as natively unfolded with random coil structures, which, in

267 PD mutants, mouse alpha-synuclein adopts a "natively unfolded" or disordered structure.

268 Barnase-GCN4 is thus a "natively unfolded" protein that uses ligand binding to s

269 hibit structural characteristics typical of "natively unfolded" proteins (highly flexible proteins th

270 der physiological conditions are said to be "natively unfolded".

271 alpha-Synuclein has long been defined as a 'natively unfolded' monomer of about 14 kDa that is belie

272 hibition domain is intrinsically disordered (natively unfolded) as shown by circular dichroism spectr

273 that Tax(59-98) is intrinsically disordered (natively unfolded) in isolation and adopts an ordered co

274 s) is highly negatively charged and probably natively unfolded, it could anchor gravin to the inner l

275 Cytosolic alpha-synuclein appears to be natively unfolded, whereas membrane-bound alpha-synuclei

276 ssociated protein that is highly soluble and natively unfolded.

277 The P protein amino-terminal domain is natively unfolded; to probe its conformational flexibili

278 Natively unstructured (also dubbed intrinsically disorde

279 ons: regular protein secondary structure and natively unstructured (intrinsically disordered) regions

280 The amphipathicity of the natively unstructured amyloid-beta (Abeta40) peptide may

281 ation and cause of the transformation from a natively unstructured assembly to the beta-sheet oligome

282 relationship between the transformation from natively unstructured assembly to the beta-sheet oligome

283 In solution, alpha-synuclein is natively unstructured but assumes an alpha-helical confo

284 ted of RraA in complex with a portion of the natively unstructured C-terminal tail of RhlB at 2.8-A r

285 ns at random coil rates, consistent with its natively unstructured condition.

286 (RTX) domains, calcium binding triggers the natively unstructured domain to adopt a beta roll struct

287 changes, consistent with these ligands being natively unstructured in the absence of beta-catenin.

288 tuated distally and dynamically because of a natively unstructured linker connecting the dimerization

289 virus 3AB is a 109-amino acid protein with a natively unstructured N-terminal domain.

290 Human alpha-synuclein (alpha-Syn) is a natively unstructured protein whose aggregation into amy

291 rmational properties of the growing class of natively unstructured proteins in biological processes.

292 stics of several types of complexes, such as natively unstructured proteins, ribosomal proteins, two-

293 Our analysis makes use of the fact that natively unstructured proteins, which undergo a disorder

294 contains a central folded domain flanked by natively unstructured regions required to facilitate mat

295 contacts has been previously used to predict natively unstructured regions.

296 NF90, a tandem pair of dsRBDs separated by a natively unstructured segment confers dsRNA binding acti

297 f roughly a dozen residues that lie within a natively unstructured sub-domain of RNase E.

298 of distributed interactions mediated by the natively unstructured termini of Hfq.

299 conformation, despite being predicted to be natively unstructured.

300 microfluidic systems and photovoltaics that natively use silicon materials, while mitigating adverse