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1 egative set and a large number of candidates necessitated the development of a new approach to identi
2 , the water solubility of a key intermediate necessitated the development of a nonextractive workup f
4 magnetic resonance spectra obtained in rigor necessitated the development of a novel decomposition te
5 rge size of this protein (55 kDa, homodimer) necessitated the development of a novel pulse sequence a
6 transgenic technology in murine species has necessitated the development of a primary mouse hepatocy
7 ellular glycosphingolipid (GSL) function has necessitated the development of a rapid and sensitive me
8 terns in a multilayer, multi-cellular tissue necessitates the development of a 3D reconstruction tech
9 at equilibrium and in transient relaxation, necessitates the development of a unifying theory which
10 ion and the increased use of marginal livers necessitate the development of accurate pretransplant te
11 and silver-resistant pathogenic strains has necessitated the development of alternative strategies.
12 programs have been only partially effective, necessitating the development of alternative mechanisms
13 vel reassortants causing a pandemic outbreak necessitate the development of an anti-influenza vaccine
15 ing the unique mechanisms of human oogenesis necessitates the development of an in vitro system of st
16 ome-scale analysis of cellular functions has necessitated the development of analytical (in silico) m
17 of the RNA polymerase II transcription cycle necessitated the development of assays that include chro
18 field from data generation to data analysis, necessitating the development of better analytical tools
19 difficult to assess at the population level, necessitating the development of biological tools to tra
20 s have long been targets for drug discovery, necessitating the development of blood assays to determi
21 that many tumor cells are CAR deficient has necessitated the development of CAR-independent infectio
24 ients benefit from antiangiogenic treatment, necessitating the development of criteria to predict the
25 F3 substituents within organic substructures necessitates the development of diverse methods for inco
27 ustrial demand for nylon-6 (polycaprolactam) necessitates the development of environmentally benign m
28 osomal deletions and loss of heterozygosity, necessitate the development of inference methods that ut
29 genotype-first" approach for complex disease necessitates the development of large, highly integrated
31 d supply of human organs for transplantation necessitates the development of methods leading to accep
32 variation observed between individual cells necessitates the development of methods that measure chr
33 s (such as inclusion of fetal bovine serum), necessitating the development of methods for generating
34 y structure emerge as a possible explanation necessitating the development of methods to measure the
35 ion that many proteins can become prions has necessitated the development of mouse models for assessi
36 mergence of multidrug-resistant mycobacteria necessitate the development of new antituberculosis drug
37 being generated and made publicly available necessitate the development of new data storage and arch
38 d the emergence of antiviral drug resistance necessitate the development of new potent antiviral drug
39 The demands of high throughput proteomics necessitate the development of new, faster experimental
40 lability of massive amounts of GWAS data has necessitated the development of new biostatistical metho
41 ghput technologies, such as DNA microarrays, necessitated the development of new computational method
42 nductors, and impact protection coatings has necessitated the development of new methodologies for de
44 tion, coupled with emerging drug resistance, necessitates the development of new drugs to treat influ
45 hed molecular heterogeneity of human cancers necessitates the development of new paradigms to serve a
47 ents tend to undergo structural degradation, necessitating the development of new amphiphilic agents
49 erential growth of resistant subpopulations, necessitating the development of next-generation therapi
50 of fungal strains exhibiting drug resistance necessitates the development of novel antimicrobial ther
51 is parasite on most known resistance sources necessitates the development of novel approaches for con
52 of bioconjugates in the biomedical sciences necessitates the development of novel mechanisms to faci
54 ncomplete and can generate loss of function, necessitating the development of novel treatment methods
55 sis and interpretation if left uncontrolled, necessitating the development of quality control protoco
56 ogy into both research and clinical settings necessitates the development of rapid and accurate means
57 ies of (211)At-labeled radiopharmaceuticals, necessitating the development of reaction conditions dif
58 isms (GMOs) in crops, foods and ingredients, necessitated the development of reliable and sensitive m
59 za virus neuraminidase (NA) inhibitors (NIs) necessitates the development of reliable methods for ass
60 Scientifically based risk assessment for MNs necessitates the development of reproducible, standardiz
61 ymes and as model experimental organisms has necessitated the development of sensitive and specific t
62 ted with Vibrio-mediated waterborne diseases necessitates the development of sensitive detection tech
63 ion of quantitative "shotgun" proteomic data necessitates the development of software to automaticall
64 logical processes involved in NGS technology necessitates the development of statistically accurate m
66 studies on microRNA regulation and function necessitates the development of suitable techniques for
67 rves and growing environmental concerns have necessitated the development of sustainable processes to
68 ft and antigenic drift of influenza viruses, necessitates the development of the next-generation of i
69 enomic and proteomic data is being generated necessitates the development of tools and resources for
70 urrent global shortage of donor corneas will necessitate the development of treatments for vision los
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