ライフサイエンスコーパス: neoblast

1 arise from a proliferative cell population (neoblasts).

2 in adult proliferating, regenerative cells (neoblasts).

3 quiring a population of proliferating cells (neoblasts).

4 sults in a dramatic reduction/elimination of neoblasts.

5 nstrate the migration and differentiation of neoblasts.

6 require a population of stem cells known as neoblasts.

7 n abundant somatic stem cell population, the neoblasts.

8 cells, is extended during the cell cycle of neoblasts.

9 lineage capacity, and can give rise to zeta-neoblasts.

10 specific cell type regeneration programs in neoblasts.

11 ions, in response to BMP signaling, required neoblasts.

12 cause of the action of dividing cells called neoblasts.

13 Planarian regeneration requires neoblasts, a population of dividing cells that has been

14 This capacity is mediated by neoblasts, a proliferative cell population that contains

15 nduced by Smed-histone-2B RNAi, resulting in neoblast ablation.

16 gene expression that are not associated with neoblast ablation.

17 stone-2B RNAi over a time course as means of neoblast ablation.

18 cent studies indicate that survival of a few neoblasts after sublethal irradiation results in the clo

19 egeneration requires adult stem cells called neoblasts and amputation triggers two peaks in neoblast

20 ly and gene expression signatures of somatic neoblasts and germline cells will be a valuable resource

21 ke (bruli) mRNA and protein are expressed in neoblasts and the central nervous system.

22 s response that induces the proliferation of neoblasts and the concomitant expansion of not only epid

23 ) and cell-cycle markers to label subsets of neoblasts and their progeny.

24 The molecular similarities between neoblasts and undifferentiated cells of other organisms

25 o, represented by somatic stem cells, called neoblasts, and germline cells.

26 symmetrically on the cytoplasmic membrane of neoblasts, and the ratio of asymmetric to symmetric cell

27 Neoblasts are adult stem cells (ASCs) in planarians that

28 Neoblasts are an abundant, heterogeneous population of a

29 Planarian neoblasts are pluripotent, adult somatic stem cells and

30 of smedwi-2 blocks regeneration, even though neoblasts are present, irradiation-sensitive, and capabl

31 ding, or during the course of cell turnover, neoblasts are signaled to divide and/or differentiate, t

32 Neoblasts are traditionally described by their morpholog

33 mediterranea embryogenesis, and report that neoblasts arise from an anarchic, cycling piwi-1+ popula

34 interference (RNAi) for genetic ablation of neoblast cells in Schmidtea mediterranea as an alternati

35 we analyze the overall expression profile of neoblast cells.

36 one-2B RNAi and validate their expression in neoblast cells.

37 population-based studies have revealed many neoblast characteristics, whether functionally distinct

38 We identified two prominent neoblast classes that we named zeta (zeta) and sigma (si

39 ikely functions as an essential regulator of neoblast clonal expansion.

40 These clonogenic neoblasts (cNeoblasts) produce cells that differentiate

41 If the neoblasts comprise a uniform population of cells during

42 These findings indicate that planarian neoblasts comprise two major and functionally distinct c

43 Dividing cells called neoblasts contain pluripotent stem cells and drive plana

44 A long-standing question is whether neoblasts directly sense and respond to the identity of

45 tology, expression of key polarity genes, or neoblast distribution.

46 lthough adult pluripotent stem cells, called neoblasts, drive long-term homeostatic tissue maintenanc

47 Planarian stem cells, or neoblasts, drive the almost unlimited regeneration capac

48 rucial role for the functionality of somatic neoblasts during homeostasis and regeneration.

49 We investigated the provenance of neoblasts during Schmidtea mediterranea embryogenesis, a

50 Zeta-neoblasts encompass specified cells that give rise to an

51 growth factor (EGF) signaling during in vivo neoblast expansion mediated by Smed-egfr-3 (egfr-3) and

52 By contrast, recent data indicate that some neoblasts express lineage-specific transcription factors

53 tion, reduced animal size, reduced number of neoblasts, fewer chromatoid bodies and increased levels

54 es show that Smed-histone-2B RNAi eliminates neoblast gene expression with high specificity and discr

55 Our list of neoblast genes parallels their morphological features an

56 Knockdown of a selected set of neoblast genes, including Mlig-ddx39, Mlig-rrm1, Mlig-rp

57 otype revealed a striking anterior-posterior neoblast gradient.

58 ched transcripts and behave differently than neoblasts in cell transplantation assays.

59 d BrdU labeling to study the distribution of neoblasts in the intact animal, as well as to directly d

60 e is not a large, slow-cycling population of neoblasts in the intact animal.

61 neoblasts to wounds, even in areas that lack neoblasts in the intact animal.

62 binding proteins is critical for regulating neoblasts in the planarian Schmidtea mediterranea.

63 ion uses a population of regenerative cells (neoblasts), including pluripotent stem cells.

64 Wound-induced gene expression in neoblasts, including that of runt-1, required SRF (serum

65 -induced genes was activated directly within neoblasts, including the Runx transcription factor-encod

66 have examined the proposal that a subset of neoblasts is arrested in the G2 phase of the cell cycle

67 A characteristic feature of neoblasts is the presence of chromatoid bodies, large cy

68 A characteristic feature of neoblasts is the presence of large cytoplasmic ribonucle

69 arge population of proliferative stem cells (neoblasts) is required for physiological tissue homeosta

70 Our analyses indicate that neoblasts lacking Bruli can respond to wound stimuli and

71 on profiling, we find that these schistosome neoblast-like cells express a fibroblast growth factor r

72 ing flatworms (for example, planarians), and neoblast-like cells have been described in some parasiti

73 Here we describe a population of neoblast-like cells in the trematode Schistosoma mansoni

74 re studies deciphering the function of these neoblast-like cells will have important implications for

75 ene is required for the maintenance of these neoblast-like cells.

76 Neoblast lineages arise as organogenesis begins and are

77 reatment inhibits regeneration and abrogates neoblast maintenance.

78 oblasts and amputation triggers two peaks in neoblast mitoses early in regeneration.

79 These cells resemble planarian neoblasts morphologically and share their ability to pro

80 ing regeneration, promoting heterogeneity in neoblasts near wounds.

81 Neoblasts normally increase eye progenitor production fo

82 n of Smed-CHD4 with RNA interference (RNAi), neoblast numbers were initially normal, despite an inabi

83 cells from diverse organisms, in particular neoblasts of planarians (free-living relatives of schist

84 nnel gene expressed in the adult stem cells (neoblasts) of the planarian Schmidtea mediterranea.

85 expressed in the dividing adult stem cells (neoblasts) of the planarian Schmidtea mediterranea.

86 Neoblasts, originally defined as undifferentiated cells

87 erous studies have examined genes underlying neoblast pluripotency, molecular pathways driving postmi

88 We characterize the neoblast population by using antibodies recognizing SMED

89 on using pluripotent adult stem cells of the neoblast population, can reversibly scale body size over

90 es should occur in multipotent, non-dividing neoblast progeny cells.

91 CHD4 was required for the formation of these neoblast progeny cells.

92 dramatic reduction in the numbers of certain neoblast progeny cells.

93 Neoblast progeny generate new cells of blastemas, which

94 ting in response to wounding; smedwi-2(RNAi) neoblast progeny migrate to sites of cell turnover but,

95 By contrast, sigma-neoblasts proliferate in response to injury, possess bro

96 homeostasis in intact animals and stem cell (neoblast) proliferation in amputated animals identifying

97 Neoblasts provide an excellent system for in vivo study

98 anisms regulating the population dynamics of neoblasts remain largely unknown.

99 onse to any injury and that a second, local, neoblast response is induced only when injury results in

100 We characterize the rapid, neoblast-specific phenotype induced by Smed-histone-2B R

101 Neoblast stem cells and progenitor cells were mislocaliz

102 sion changes occurred even in the absence of neoblast stem cells, which are required for regeneration

103 lities of freshwater planarians are based on neoblasts, stem cells maintained throughout the animal's

104 rians, pluripotent somatic stem cells called neoblasts supply new cells for growth, replenish tissues

105 and single-cell transplantation to identify neoblasts that can form large descendant-cell colonies i

106 d to a population of adult stem cells called neoblasts that proliferate and differentiate to produce

107 ms contain a population of adult stem cells (neoblasts) that proliferate and generate cells of all ti

108 In addition to requiring new cells (from neoblasts), these feats require mechanisms that specify

109 e molecularly and functionally distinct from neoblasts: they express unique cohorts of early embryo e

110 However, the proliferative response of neoblasts to amputation or growth stimulation in Smed-CH

111 analogue bromodeoxyuridine (BrdU), allowing neoblasts to be labeled specifically during the S phase

112 al profiling from over a thousand individual neoblasts to directly compare gene expression fingerprin

113 val was neither sufficient nor necessary for neoblasts to increase eye progenitor production.

114 ults indicate that Smed-CHD4 is required for neoblasts to produce progeny cells committed to differen

115 narian regeneration is the specialization of neoblasts to produce specified rather than naive blastem

116 gest that SMEDWI-2 functions within dividing neoblasts to support the generation of cells that promot

117 response was characterized by recruitment of neoblasts to wounds, even in areas that lack neoblasts i

118 It is unknown how the collective output of neoblasts transit through differentiation pathways to pr

119 Subsequently, these neoblasts were induced to divide and differentiate near

120 ies as early in the lineage as the epidermal neoblasts, with further pre-patterning occurring in thei