ライフサイエンスコーパス: neocarzinostatin

1 characterized in the biosynthetic pathway of neocarzinostatin.

2 alyzed activation of the enediyne antibiotic neocarzinostatin.

3 gamma irradiation or the radiomimetic agent neocarzinostatin.

4 cells sensitive toward a radiomimetic agent, neocarzinostatin.

5 hydroxyurea, or the radiomimetic antibiotic, neocarzinostatin.

6 t 5-fold more cytotoxic than that induced by neocarzinostatin.

7 ced by the DNA-damaging agents etoposide and neocarzinostatin.

8 D, anisomycin or with the radiomimetic drug neocarzinostatin.

9 ge induced by antitumor enediynes C-1027 and neocarzinostatin.

10 ith gamma-radiation or the radiomimetic drug neocarzinostatin.

11 poptosis and differentiation by the enediyne neocarzinostatin.

12 Neocarzinostatin, a clinical anticancer drug, is the arc

13 of HCT116 cells with a radiomimetic compound neocarzinostatin, active Chk2 exists as stable Thr-68-ph

14 y that can now be exploited to produce novel neocarzinostatin analogs by engineering neocarzinostatin

15 rative studies with other enediynes, such as neocarzinostatin and calicheamicin gamma 1I under simila

16 ation chemistry of the enediyne antibiotics, neocarzinostatin and calicheamicin gamma(1)(I), was expl

17 DNA and DNA-RNA hybrids by four drugs: BLM, neocarzinostatin and esperamicins A1 and C.

18 ored resistance to gamma-rays, bleomycin and neocarzinostatin, and also restored DSB-repair proficien

19 as most of the anaerobic lesions produced by neocarzinostatin are drug monoadducts.

20 ell kill, ionizing radiation, bleomycin, and neocarzinostatin are equally effective at eliciting dela

21 ovel neocarzinostatin analogs by engineering neocarzinostatin biosynthesis or applying directed biosy

22 We have previously cloned and sequenced the neocarzinostatin biosynthetic gene cluster and proposed

23 dependent O-methyltransferase (NcsB1) in the neocarzinostatin biosynthetic pathway.

24 nsisting of three from 9-membered producers (neocarzinostatin, C1027, and maduropeptin) and three fro

25 ctive synthetic route to the natural product neocarzinostatin chromophore (1) is described.

26 g agents, including gamma-radiolysis and the neocarzinostatin chromophore (NCS).

27 al covalent adduct formed by thiol-activated neocarzinostatin chromophore (NCS-chrom) and a RNA-DNA h

28 on of the complexes of DNA and postactivated neocarzinostatin chromophore (NCS-chrom) compounds revea

29 Neocarzinostatin chromophore (NCS-chrom) has been found

30 argets for the enediyne antitumor antibiotic neocarzinostatin chromophore (NCS-chrom) in a base-catal

31 Neocarzinostatin chromophore (NCS-Chrom) induces highly

32 Neocarzinostatin chromophore (NCS-Chrom) induces strong

33 ained from the enediyne antitumor antibiotic neocarzinostatin chromophore (NCS-chrom), we have prepar

34 f the product epoxy alcohol (58) then formed neocarzinostatin chromophore aglycon (2, 71% yield).

35 ynthesis of the naphthoic acid moiety of the neocarzinostatin chromophore and further support the ear

36 c acid moiety and its incorporation into the neocarzinostatin chromophore are catalyzed by five enzym

37 The neocarzinostatin chromophore consists of a nine-membered

38 argets for the enediyne antitumor antibiotic neocarzinostatin chromophore in a base-catalyzed, radica

39 own in a previous study that thiol-activated neocarzinostatin chromophore is able to cleave duplex DN

40 observations that treatment of DNA with the neocarzinostatin chromophore resulted in large amounts o

41 aphthoic acid and its incorporation into the neocarzinostatin chromophore with free naphthoic acids s

42 Under anaerobic conditions neocarzinostatin chromophore, an enediyne antibiotic, fo

43 cule DNA cleaving agents, such as bleomycin, neocarzinostatin chromophore, and lomaiviticin A, in pla

44 analogue 3, an alpha-D-fucose derivative of neocarzinostatin chromophore, formed in 42% yield by a t

45 pared from the enediyne antitumor antibiotic neocarzinostatin chromophore.

46 previously developed for the synthesis of a neocarzinostatin core model (4) failed in the requisite

47 f the DNA bulge-specific enediyne antibiotic neocarzinostatin, differs in possessing a more stable sp

48 C-1027 and neocarzinostatin doses that caused similar levels of DNA

49 f deoxyribose in DNA, caused by the enediyne neocarzinostatin, for example, acylate the N(6)-amino gr

50 re to various DSB-inducing agents, including neocarzinostatin, gamma rays, and etoposide, and found t

51 gamma-irradiation and the radiomimetic drug neocarzinostatin had no effect on ATM protein levels, in

52 different strand-breaking agents, bleomycin, neocarzinostatin, hydrogen peroxide, restriction endonuc

53 s, for studies of the mechanism of action of neocarzinostatin in vivo.

54 In contrast, neocarzinostatin-induced reciprocal exchange mutants, as

55 mple, the conventional radiomimetic enediyne neocarzinostatin is 4-fold less cytotoxic to cells maint

56 Neocarzinostatin (NCS) is an antitumor antibiotic compri

57 n of apoptosis in PC12 cells by the enediyne neocarzinostatin (NCS) is paradoxically potentiated by o

58 The natural product neocarzinostatin (NCS), a protein-small molecule complex

59 biosynthesis of the naphthoic acid moiety of neocarzinostatin (NCS), we expected a biosynthetic pathw

60 radiation or radiomimetic chemicals, such as neocarzinostatin (NCS), which induce DNA DSBs.

61 ated that Bcl-2 has a proapoptotic effect on neocarzinostatin (NCS)-treated PC12 pheochromocytoma cel

62 ncluding the DNA-cleaving antimitotic agent, neocarzinostatin (NCS).

63 Bs induced by the non-protein chromophore of neocarzinostatin (NCS-C) in the human Alu repeat.

64 Neocarzinostatin (NCS-chrom), a natural enediyne antitum

65 e enediyne PKS involved in C-1027 (SgcE) and neocarzinostatin (NcsE) biosynthesis has now revealed th

66 adiomimetic DNA double-strand cleaving agent neocarzinostatin, nine were large-scale rearrangements.

67 o paraquat and H2O2, but not to hydroxyurea, neocarzinostatin, or camptothecin.

68 The naphthoic acid moiety of the enediyne neocarzinostatin plays key roles in the biological activ

69 27 producer Streptomyces globisporus and the neocarzinostatin producer Streptomyces carzinostaticus u

70 )(I), was exploited in control studies, with neocarzinostatin producing 10 2-deoxyribonolactone and 3

71 Transcription profiling experiments with neocarzinostatin protein alone implicate a specific resi

72 Cells treated with neocarzinostatin showed a clear dose-response relationsh

73 For neocarzinostatin, the sequence specificity lies in the A

74 by all criteria tested like active Chk2 from neocarzinostatin-treated HCT116 cells.

75 double-strand cleaving agents bleomycin and neocarzinostatin were more mutagenic in plateau-phase th

76 d hypersensitivity to x-rays, bleomycin, and neocarzinostatin, which all induce 3'-phosphoglycolate-t