ライフサイエンスコーパス: neogenin

1 ts that membrane HJV shedding is mediated by neogenin.

2 Type 2A HH did not co-immunoprecipitate with neogenin.

3 e secreted factor netrin-3 and its receptor, neogenin.

4 the Deleted in Colorectal Cancer protein and Neogenin.

5 hat specifically lowers its interaction with neogenin.

6 rs DCC (deleted in colorectal carcinoma) and Neogenin.

7 ubiquitously expressed cell surface receptor neogenin.

8 ds both bone morphogenic proteins (BMPs) and neogenin.

9 ectors is dependent on its interactions with neogenin.

10 ligands and BMP receptors, independently of neogenin.

11 res it to bind to the transmembrane receptor neogenin.

12 Neogenin, a deleted in colorectal cancer (DCC) family me

13 study showing that knock-down of endogenous neogenin, a HJV receptor, in C2C12 cells suppresses HJV

14 HJV also binds neogenin, a membrane protein widely expressed in many ti

15 Neogenin, a protein with nearly 50% amino acid identity

16 Lrig2 binds Neogenin, a receptor for repulsive guidance molecules (R

17 HJV co-immunoprecipitated with neogenin, a receptor involved in a variety of cellular s

18 HJV also interacts with neogenin, a ubiquitously expressed transmembrane protein

19 Together these data support a model in which neogenin acts as a scaffold to facilitate assembly of th

20 nin interactions, providing ADAM17 access to Neogenin and allowing this protease to induce ectodomain

21 roteins, including the cell surface receptor neogenin and bone morphogenetic protein (BMP) cytokines,

22 s a GPI-anchored protein, binds the proteins neogenin and bone morphogenetic proteins (BMP2 and BMP4)

23 ng cells also expressed the netrin receptors neogenin and Deleted in Colorectal Carcinoma (DCC).

24 The identification of human neogenin and its chromosomal location provides a basis f

25 Specifically, neogenin and restin were found to exhibit proproliferati

26 Antisense ODNs to neogenin and restin, but not an antisense ODN to rap1GAP

27 nositol-anchored protein that interacts with neogenin and suppresses its secretion.

28 lease is coupled to lysosomal degradation of neogenin and that cholesterol depletion by filipin block

29 e observed a cytoplasmic staining pattern of neogenin and UNC5A/B that also increased following activ

30 a-/- mice include c-Jun N-terminal kinase 2, neogenin, and gephyrin; the latter encodes a protein tha

31 ncoding GTPase activating protein (rap1GAP), neogenin, and restin in HOSE and OVCA cells.

32 Interestingly, dynorphin, neogenin, and synaptotagmin VII, genes that possess cAMP

33 ceptors [deleted in colorectal cancer (DCC), neogenin, and the adenosine A2b receptor] are expressed

34 eceptors DCC (deleted in colorectal cancer), Neogenin, and Unc-5.

35 vates FAK and ERK in cultured myoblasts in a neogenin- and Cdo-dependent manner, whereas recombinant

36 eleted in colorectal cancer) family [DCC and neogenin] and the UNC5 family (UNC5H1, UNC5H2 and UNC5H3

37 Netrin and its receptor Neogenin are thought to be regulators of axonal guidance

38 Although Netrin receptors Unc5B and neogenin, are expressed by human lymphatic endothelial c

39 Our finding that BMP-2 and neogenin bind simultaneously to hemojuvelin raises the p

40 Furthermore, neogenin binds to CDO in a cis fashion, and myoblasts la

41 Neogenin binds to cleaved and noncleaved hemojuvelin, as

42 otein, transferrin receptor 2, matriptase-2, neogenin, BMP receptors, and transferrin.

43 HJV has also been demonstrated to bind to neogenin, but it is not known whether this interaction h

44 Regulation of ADAM17-mediated Neogenin cleavage by Lrig2 is required for neurite growt

45 Further studies revealed that neogenin co-immunoprecipitated with ALK3, an essential t

46 in-mediated HJV release occurs after the HJV-neogenin complex is internalized from the cell surface.

47 ated protein Cdo (also Cdon) was a potential neogenin coreceptor in myoblasts.

48 Knockdown of neogenin decreased BMP4-induced hepcidin mRNA levels by

49 Wid-type, neogenin deficient and chimeric mice.

50 to Smad, but not p38 MAPK, was diminished in neogenin-deficient chondrocytes.

51 Neogenin-deficient mice were impaired in digit/limb deve

52 ne-trap mutation in the Neo1 locus (encoding neogenin) develop myotomes normally but have small myofi

53 Neogenin does not, however, play a role in HJV trafficki

54 The addition of a soluble neogenin ectodomain to cells markedly inhibits HJV relea

55 rs of magnitude more tightly than the entire neogenin ectodomain.

56 n of netrin-1 canonical receptors, Unc5B and neogenin, expressed by lymphatic endothelial cells, do n

57 skin enhanced inflammation and the number of neogenin-expressing CD3(+) T cell infiltrates.

58 Neogenin expression was evaluated during inflammatory st

59 verexpression or by inhibition of endogenous neogenin expression.

60 ssion of soluble versions of hemojuvelin and neogenin for biochemical characterization of their inter

61 These results suggest that neogenin forms a ternary complex with both MT2 and HJV a

62 pattern to that of hepcidin, suggesting that neogenin functions in a cell nonautonomous manner.

63 e deleted in colorectal cancer (DCC) homolog neogenin functions in both netrin- and repulsive guidanc

64 How neogenin functions in mediating BMP signaling is not wel

65 The chromosomal location of the human neogenin gene (HGMW-approved symbol NEO1) was determined

66 Neogenin has also been shown to facilitate the cleavage

67 Neogenin has been identified as a receptor for the neuro

68 However, the role of the neogenin-HJV interaction in the function of HJV is unkno

69 We show that the sole C. elegans DCC/neogenin homolog UNC-40 positively modulates a BMP-like

70 sses HJV shedding and that overexpression of neogenin in HEK293 cells markedly enhances this process,

71 In this study we characterized the role of neogenin in HJV-regulated hepcidin expression.

72 l-length neogenin with a soluble fragment of neogenin in mice.

73 Here we provide evidence for neogenin in regulating endochondral bone development and

74 ey 293 cells is dependent on the presence of neogenin in the cells, thus linking these two proteins t

75 In this study, we showed that neogenin interacted with MT2 as well as with HJV and fac

76 Together, these results suggest that the HJV-neogenin interaction is required for the BMP-mediated in

77 studies indicated that disruption of the HJV-neogenin interaction is responsible for a marked suppres

78 Furthermore, RGMs appear to mediate neogenin interaction with BMP receptors in chondrocytes.

79 vely, our findings demonstrate that Netrin-1/neogenin interactions augment CD4(+) T cell chemokinesis

80 RGMa reduces Lrig2-Neogenin interactions, providing ADAM17 access to Neogen

81 on the plasma membrane, suggesting a lack of neogenin involvement in their trafficking to the cell su

82 Neogenin is a receptor for ligands of the netrin and rep

83 We provide evidence that its close relative neogenin is also a functional netrin-1 receptor that act

84 Neogenin is also expressed in other embryonic tissues, s

85 The ectodomain of neogenin is composed of four immunoglobulin-like (Ig) do

86 cidin mRNA, suggesting that interaction with neogenin is critical for the iron regulatory function of

87 ressed in prelumenal cells, and its receptor neogenin is expressed in a complementary pattern in adja

88 Neogenin is expressed in liver cells in a reciprocal pat

89 y to hemojuvelin raises the possibility that neogenin is part of a multiprotein complex at the hepato

90 Neogenin is required for the processing and release of H

91 The increase in MT2 and HJV upon neogenin knockdown was likely due to the inhibition of c

92 Mouse neogenin lacking the intracellular domain is also capabl

93 Knockdown of endogenous neogenin markedly suppresses HJV release but has no evid

94 ding to the most membrane-proximal region of neogenin may play a role in regulating the levels of sol

95 It is proposed that netrin-3 and neogenin may promote myogenic differentiation by an auto

96 Further studies demonstrate that neogenin may stabilize HJV, a glycosylphosphatidylinosit

97 HJV endocytosis and HJV release suggest that neogenin-mediated HJV release occurs after the HJV-neoge

98 Cell aggregation assays demonstrate that neogenin mediates netrin-1-dependent cell clustering.

99 evidence indicates that the netrin receptor, Neogenin, mediates netrin signaling in vascular smooth m

100 Livers of neogenin mutant mice exhibit iron overload, low levels o

101 activation, was reduced in chondrocytes from neogenin mutant mice.

102 ical role for the neuronal guidance receptor neogenin (Neo1) outside the nervous system in mediating

103 through their common cell-surface receptor, neogenin (NEO1).

104 ted in mice with gene targeted repression of neogenin (Neo1-/-), bone marrow chimeric animals and con

105 To explore a role for the human neogenin (NGN) gene in cancer, we have isolated cDNAs fo

106 netrin-1 region, alone and in complexes with neogenin or DCC.

107 DCC (deleted in colorectal cancer), but not neogenin or Unc5h2.

108 gh expression of UNC5B but not UNC5C, UNC5D, neogenin, or deleted in colorectal cancer.

109 g and hepcidin expression are not altered by neogenin overexpression or by inhibition of endogenous n

110 for SCI and uncovers a new role for the RGMa/Neogenin pathway on neuropathic pain.

111 Radiation hybrid mapping of Nope, Punc, and Neogenin placed all three genes in close vicinity on mou

112 Here we provide evidence that neogenin plays a critical role in iron homeostasis by re

113 The role that neogenin plays in HJV trafficking was investigated, usin

114 us studies, our results support that hepatic neogenin possesses two functions, mediation of cellular

115 We reported previously that neogenin promoted myotube formation by C2C12 myoblasts i

116 Taken together, our results indicate that neogenin promotes chondrogenesis in vitro and in vivo, r

117 Like DCC, the neogenin protein consists of four immunoglobulin-like (I

118 quencing of cDNA clones coding for the human neogenin protein.

119 sults suggest that netrin-1 and its receptor neogenin provide an adhesive, rather than a guidance, fu

120 were mediated via binding of Netrin-1 to the Neogenin receptor and activation of SHP-2, resulting in

121 erting its repulsive activity by binding the Neogenin receptor.

122 ting as an axon guidance cue through Dcc and neogenin receptors, it is also thought to regulate neuro

123 DCC and neogenin, receptors implicated in mediating the attracta

124 gether, our results lead the hypothesis that neogenin regulates iron homeostasis via inhibiting secre

125 ings reveal an unexpected mode of action for neogenin regulation of BMP signaling.

126 revealing an unexpected mechanism underlying neogenin regulation of BMP signaling.

127 results reveal a novel mechanism underlying neogenin regulation of HJV-BMP signaling.

128 Human neogenin shares 87% identity with its chicken homolog, a

129 nce molecules (RGMs), and prevents premature Neogenin shedding by ADAM17 (TACE).

130 Together, netrin-neogenin signaling is an important extracellular cue in

131 4 (FN4) and FN5, which differs among DCC and neogenin splice variants, providing a basis for diverse

132 hus, netrin-1 appears to act locally through neogenin to stabilize the multipotent progenitor (cap) c

133 We localized the hemojuvelin binding site on neogenin to the membrane-proximal fifth and sixth FNIII

134 Neogenin, uncoordinated-5 (UNC5)A, and UNC5B were expres

135 Neogenin, unlike the CAMs, is closely related to a uniqu

136 Neogenin was also observed on CD3(+) T cell infiltrates

137 Neogenin was first identified in the chick embryo, and l

138 In contrast, neogenin was not cleaved by MT2, indicating some degree

139 In addition, functional inhibition of neogenin was performed using antibody injection.

140 Both HJV and neogenin were expressed in liver hepatocytes.

141 king the interaction of HJV with full-length neogenin with a soluble fragment of neogenin in mice.

142 Down-regulation of neogenin with siRNA increased the amount of MT2 and HJV