ライフサイエンスコーパス: nested PCR

1 (hence, the term merged tandem-nested or M/T-nested PCR).

2 on, and junction fragments were amplified by nested PCR.

3 ules for each clone type using two rounds of nested PCR.

4 plified from individually dissected cells by nested PCR.

5 , viral genomes were no longer detectable by nested PCR.

6 HTLV-1 tax had been periodically detected by nested PCR.

7 r the presence of RRV by virus isolation and nested PCR.

8 rious depths and the dorsum of the tongue by nested PCR.

9 2 and 6 of the PIG-A gene were amplified by nested PCR.

10 be used to detect Cryptosporidium parvum by nested PCR.

11 and tested them for pig DNA and PERV DNA by nested PCR.

12 -negative samples (88%) were negative in the nested PCR.

13 blood of subclinically infected mice by the nested PCR.

14 A, 25 patients (26%) were positive by the RT-nested PCR.

15 The samples were tested by nested PCR.

16 plified in Ehrlichia canis-infected cells by nested PCR.

17 dorferi from infected ticks was amplified by nested PCR.

18 00% when discrepant samples were retested by nested PCR.

19 s needed for sample processing and multiplex nested PCR.

20 city of LAMP results, compared with those of nested PCR.

21 multiplex assay platforms without the use of nested PCR.

22 r detection of adenoviruses was performed by nested PCR.

23 to perform two to three successive rounds of nested PCR.

24 in 81 of 180 (45%) of individuals using semi-nested PCR.

25 e-specific RT-PCR and real-time quantitative nested PCR.

26 ymptomatic staff and patients were tested by nested PCR.

27 ng HIV PT and RT sequences were amplified by nested PCRs.

28 61%; 95% CI, 53 to 69%; P = 0.007) and ITS-1 nested PCR (54%; 95% CI, 45 to 62%; P < 0.001); real-tim

29 This demonstrated that the nested PCR achieved very nearly the maximum theoreticall

30 Nested PCR also detected M. pulmonis in 14 of 20 (70%) p

31 Nested PCR also detected M. pulmonis in 21 of 61 (34%) C

32 cted to nucleotide sequence analysis using a nested PCR amplification approach for each gene.

33 were determined after reverse transcriptase-nested PCR amplification of viral RNA directly from rumi

34 The sensitivity of the nested PCR amplification reaction was one organism.

35 A second round of nested PCR amplification was performed in the presence o

36 The nested PCR amplified integrated proviral DNA in nucleic

37 Two-stage nested PCR analysis and DNA sequencing confirmed the int

38 Nested PCR analysis at the SAG2 locus provides rapid ass

39 The multiplex nested PCR analysis described here will be useful for an

40 y the 50% tissue culture infectious dose and nested PCR analysis of proviral DNA.

41 Multiplex nested PCR analysis was used to genotype parasites in ce

42 In summary, RT-nested PCR and a commercially available RT-PCR assay for

43 tudy was to compare the sensitivities of the nested PCR and cell culture with that of the indirect fl

44 n fragments into 96-well plates, followed by nested PCR and DNA sequencing, was used to determine the

45 of E. canis nonendemicity) were examined by nested PCR and immunofluorescent-antibody (IFA) test.

46 ce of selected organisms were examined using nested PCR and multiplexed bead-based flow cytometry.

47 virus and shellfish samples, application of nested PCR and nucleotide sequencing, and increased know

48 HHV8-seropositive donor samples by in-house nested PCR and quantitative real-time PCR assays, respec

49 assay offers an alternative to conventional nested PCR and restriction fragment length polymorphism

50 action (PCR) and sequencing, and for RSTs by nested PCR and restriction fragment length polymorphism

51 fic polymerase chain reaction (RS-PCR), semi-nested PCR and RNase protection assay.

52 Nested PCR and Southern blot analysis confirmed the iden

53 se results by using single-cell primer-based nested PCRs and Sanger sequencing.

54 HGE agent no. 13 isolate as the antigen, by nested PCR, and by culture.

55 d studied by immunochemistry, real-time PCR, nested PCR, and in situ hybridization to identify NKT ce

56 clinic and compared with expert microscopy, nested PCR, and quantitative PCR (qPCR).

57 enes from single E4+ cells were amplified by nested PCR, and the amplified products were sequenced di

58 A new three-phase, double-nested PCR approach allowed robust melting temperature a

59 In vivo, as detected by a very sensitive nested PCR approach, methylation of the discrete AP-2alp

60 By using an inverse nested PCR approach, we found that the VPI-2 region can

61 thylated DNA, which normally would require a nested PCR approach.

62 However, a nested-PCR approach using non-degenerate MIH-specific pr

63 vity and specificity were calculated using a nested PCR as the gold standard and the novel primer set

64 by comparing with an 18S ribosomal RNA-based nested PCR as the gold standard.

65 lls (PBMCs) from 68 of 101 patients (67%) by nested PCR, as compared with 8 of 218 (3.7%) healthy con

66 The new 23S-5S IGS nested PCR assay amplified all 11Rickettsiaspp., but the

67 We compared a nested PCR assay and microscopic examination of Giemsa-s

68 gonucleotide primer design and seminested or nested PCR assay design were used to enhance the breadth

69 The nested PCR assay had an overall sensitivity of 42.5%, a

70 MRV gag and env and one sensitive, published nested PCR assay targeting env.

71 Here we describe a fluorescence-monitored, nested PCR assay that is able to quantify the relatively

72 This study describes the development of a nested PCR assay that uses a unique element (ISMap02) fo

73 lot assay to detect antibodies to NWM SFV, a nested PCR assay to detect NWM SFV DNA, and a beta-galac

74 A newly described nested PCR assay was capable of amplifying genomic DNA f

75 A sensitive and specific nested PCR assay was developed for the detection of gran

76 A nested PCR assay was developed to analyse the appearance

77 In this study, a nested PCR assay was developed to detect C. parvum DNA d

78 A novel nested PCR assay was developed to detectRickettsiaspp. i

79 A nested PCR assay was used to analyze the appearance of t

80 nodes of seven (20%) deer were positive in a nested PCR assay with E. chaffeensis-specific primers.

81 detect RNA extracted from the samples: an RT-nested PCR assay with primers derived from the 5' noncod

82 mal cervical smears, a reverse transcription-nested PCR assay with primers from the E5 open reading f

83 an indirect fluorescent antibody test and a nested PCR assay, respectively.

84 We used a nested PCR assay, which reproducibly detected a 10(4)- t

85 rotoxin detection by cytotoxin assay and the nested PCR assay.

86 done using an in-house reverse transcriptase-nested PCR assay.

87 1/8 using the COBAS assay and 6/8 using the nested PCR assay.

88 In the present study, a nested-PCR assay targeting the Tso31 gene was developed

89 was measured using a quantitative real-time nested-PCR assay, and the specificity of directed integr

90 Using a nested-PCR assay, we found that both DeltaM83 and DeltaM

91 s comparable to that of previously described nested PCR assays (A. phagocytophilum, 16S rRNA; B. burg

92 s real-time PCR assay offers advantages over nested PCR assays and may improve the detection of C. pn

93 showed both assays to be more sensitive than nested PCR assays currently in use at the CDC.

94 me PCR demonstrates greater sensitivity than nested PCR assays in FFPE tissues and provides an effect

95 monkeys were evaluated for XMRV infection by nested PCR assays with nucleotide sequence confirmation,

96 mia (CMV-Ag) assay, one or more in-house CMV nested PCR assays, and/or patient evaluation and follow-

97 compared to the results of culturing and two nested PCR assays, targeting the 16S rRNA and ompA genes

98 Oligonucleotide primers were developed for nested PCR based on Chlamydia, Ureaplasma, and Neisseria

99 We describe here a nested PCR-based strategy for genome walking to extend a

100 with parasitemia levels detectable by 3-well nested PCR but very low or undetectable by qPCR.

101 . pulmonis strain, 14 (19%) were positive by nested PCR, but only 2 of 72 (2.8%) were positive by cul

102 ands appeared in most cases after performing nested PCR casting doubt on the physiologic relevance of

103 of the ASCs are then amplified by RT-PCR and nested PCR, cloned into expression vectors and transfect

104 Nested PCR cloning from an S. gordonii library enabled t

105 Nested-PCR cloning enabled the isolation of a 324-bp-lon

106 r the detection of both point mutations uses nested PCR combined with restriction enzyme digestions,

107 Strain-specific nested PCR confirmed that the superinfecting strain was

108 Having established that the nested PCRs could detect single molecules of target sequ

109 f 20 replicates, using 12 outer and 28 inner nested PCR cycles, with an intervening UNG digestion ste

110 The RS-PCR and the semi-nested PCR demonstrated products that co-migrated with t

111 f the 5'NC and NS3 sequences amplified by RT-nested PCR demonstrated that all but two positive patien

112 The Tso31 nested PCR described here might be a useful tool for the

113 Conventional nested PCR detected herpesviral DNA in brain tissue samp

114 The single-tube nested PCR did not amplify P. carinii isolated from rats

115 ipts detectable by reverse transcription-PCR/nested PCR (e.g., PDX-1, PAX-4, PAX-6, Nkx2.2 and Nkx6.1

116 By the nested PCR, E. risticii was detectable in the blood and

117 The real-time VD4 assay and one nested PCR each detected C. pneumoniae in a single, but

118 gene (CP1-CP2/CPC-CPD) was used to perform a nested PCR, followed by confirmation of the findings wit

119 Each nucleus was assayed by nested PCR for cccDNA and for cellular IFN-alpha genes a

120 on the 16S rRNA gene relative to that of the nested PCR for detection of E. chaffeensis in infected D

121 HIV RNA could not be detected in plasma, and nested PCR for HIV RNA and DNA on bulk PBMCs and sigmoid

122 These findings were confirmed by nested PCR for SIVgag DNA in brain and RT-PCR for viral

123 The results were compared to those of a nested PCR for the detection of HCMV glycoprotein B DNA

124 ere consistently found within lesions, and a nested PCR for the rRNA gene also demonstrated the prese

125 oratory between May, 1994, and May, 1996, by nested PCR for viruses associated with CNS infections in

126 ay (ELISA) from Techlab, using real-time and nested-PCR for Entamoeba species to resolve any discrepa

127 rimer pairs, HPV sequences were amplified by nested PCR from DNA isolated from cervical smear samples

128 Nested PCR from spiked urine samples detected 1 to 10 co

129 The nested PCR generated a strong signal from a minimum of 0

130 ication method, and for HCMV and EBV-1 using nested PCR identification.

131 mpared among LAMP-QCM, conventional LAMP and nested PCR in 50 cervical cancer tissues.

132 es, we detected JCV regulatory region DNA by nested PCR in 6/19 (32%) HIV-positive PML patients, 2/11

133 d sensitivity similar to that of single-well nested PCR in a United Kingdom reference laboratory.

134 rent infections with CAV-1, as detected by a nested PCR, in a range of samples, including liver, kidn

135 xpression by reverse transcriptase-dependent nested PCR, including DNA sequence analysis, in situ hyb

136 e virus-cell junctions detectable by inverse nested PCR (invPCR).

137 The study indicated that the nested PCR is as sensitive as culture for detecting infe

138 Our results indicate that the nested PCR is highly sensitive and specific for detectio

139 Our results demonstrate that the single-tube nested PCR is ideally suited for (i) diagnostic testing

140 Nested PCR is not an adequate method for identifying DNA

141 We conclude that nested PCR is superior to single PCR or culture for dete

142 set of four highly sensitive and polymorphic nested PCR markers.

143 By use of a very sensitive nested PCR method targeting part of the strongly conserv

144 Here, a nested PCR method targeting the internal transcribed spa

145 d respiratory (CAR) bacillus, we developed a nested PCR method using primers for 16S rRNA gene sequen

146 Furthermore, a new nested-PCR method for the detection of morbillivirus is

147 The results were compared to those of a nested-PCR method targeting the insertion sequences IS48

148 A consensus nested-PCR method was designed for investigation of the

149 I: 90.40-100%) compared to the gold standard nested-PCR method.

150 eritonitis virus (FIPV) infection based on a nested PCR (nPCR) assay was developed and tested with FI

151 Previously, boar semen was tested by a nested PCR (nPCR) assay which was compared to the "gold

152 up to 500 m outside hotspots, determined by nested PCR (nPCR) at baseline and 8 wk (16 June-6 July 2

153 pared RTQ-PCR to microscopy of blood smears, nested PCR (nPCR), and parasite circulating-antigen (CAg

154 Nested PCRs (nPCRs) for amplification of Neisseria menin

155 and evidence of Pneumocystis colonization by nested PCR of BAL fluid.

156 Pooled Chelex extraction of DNA, followed by nested PCR of cytochrome b, was the optimal strategy, al

157 The primers used for nested PCR of the HIV-1 pol gene amplified templates fro

158 esting for HIV, including plasma HIV RNA and nested PCR on bulk peripheral blood mononuclear cells (P

159 Peptide expression was verified using nested PCR on template cDNA derived from mRNA extracted

160 The nested PCR outperformed conventional microscopic diagnos

161 Nine sets of nested PCR primers from a 2.6-kb region of the hepatitis

162 ied by ligation-mediated PCR (LM-PCR), using nested PCR primers to increase the specificity and sensi

163 nces of contamination were in the context of nested PCR procedures.

164 were alkaline lysed and after two rounds of nested PCR products were recovered and directly sequence

165 bjected to successive amplifications using a nested PCR protocol, followed by sequencing.

166 or the presence of C. pneumoniae DNA using a nested-PCR protocol targeting a species-specific gene se

167 ing primer walking, allele-specific PCR, and nested PCR provide specialized validation and detection

168 from the total DNA of individuals by use of nested PCR reactions, and the resulting 430-bp fragment

169 ol of gene-specific primers followed by semi-nested PCR resulted in a significant increase in sensiti

170 a first reverse primer followed by two semi-nested PCR rounds using primers that are each time neste

171 We developed a reverse transcriptase nested PCR (RT-nPCR) assay for the detection of viral ga

172 In a more sensitive nested PCR, samples from 18 of 30 (60%) MM patients were

173 In this study, we performed nested-PCR screening and identified bufavirus from 12 me

174 transcripts, the primers used for real-time nested PCR spanned the splice sites.

175 A nested PCR specific for the Mycoplasma pneumoniae P1 gen

176 Remarkably, even a highly sensitive semi-nested PCR, specific for the CLL-expressed IGHV1-69/IGHD

177 non we encountered in the development of our nested PCR-SSP typing system for HLA class II alleles.

178 he addition of a primary amplification step (nested PCR-SSP).

179 sites, we conducted Plasmodium cytb-specific nested PCR surveys using blood from water buffalo in Vie

180 taminating proviral DNA was detected using a nested PCR targeting the Alu repeat in human genomic DNA

181 qualitative polymerase chain reaction (PCR) (nested PCR targeting the cytochrome b gene) and quantita

182 months after transplantation by a two-stage nested PCR technique to detect donor MHC HLA DR gene spe

183 We describe a degenerate nested PCR technique with the capacity to detect a broad

184 gocytophila genogroup rickettsiae by using a nested PCR technique.

185 Assays for HIV-1 DNA were done by using nested PCR techniques that amplify HIV-1 gag DNA from bl

186 Taken together, the two nested PCR tests on the subset of 21 culture-positive sa

187 In nested PCR tests using the 16S rRNA gene, the DNA of the

188 The nested PCR that we used can detect 2.4 copies of KSHV se

189 Upon reverse transcription of the RNA and nested PCR, the procedure detected C. albicans "housekee

190 In comparison with nested PCR, the sensitivity and specificity of RealAmp i

191 Compared with 3-well nested PCR, the sensitivity of both LAMP and single-well

192 Compared with nested PCR, the sensitivity, specificity, positive predi

193 Their Igs were amplified from cDNA using nested PCR, then cloned and sequenced.

194 We used semi-nested PCR to amplify TTV DNA from serum samples from 12

195 protein (PifC) from R. sphaeroides, we used nested PCR to clone and characterise the encoding gene,

196 We developed a nested PCR to detect the enterotoxin gene of B. fragilis

197 nneuronal fractions, followed by primary and nested PCR to quantitate VZV DNA at the single cell leve

198 V gene sequence (KS330233) was negative, but nested PCR to yield a final product of 186 bp internal t

199 rimers were then used to perform a two-step (nested) PCR to amplify the K9-specific rat testicular RN

200 roach, hybridization-enriched templates with nested PCR, to detect microclones with Ig alpha/c-myc re

201 cDNA was cloned and sequenced by RT-PCR and nested PCR using degenerated oligonucleotides.

202 for Ehrlichia risticii, the agent of PHF, by nested PCR using primers specific to the 16S rRNA gene.

203 Eleven spore trap samples were analyzed by nested PCR, using oligonucleotide primers designed for t

204 d newly developed 'asymmetry linker-mediated nested PCR walking' (ALN-walking) for CNV breakpoint seq

205 The sensitivity of multiplex nested PCR was >/=25 parasites/ml in amniotic fluid and

206 absolute detection limit of the single-tube nested PCR was 1 organism, while the practical detection

207 the sensitivity of both LAMP and single-well nested PCR was 90%; the microscopy sensitivity was 51%.

208 Nested PCR was also used to detect H. pylori from beneat

209 A nested PCR was applied to amplify a segment of open read

210 araffin-embedded specimens from 37 dogs, and nested PCR was attempted on DNA from 9 fresh tissue spec

211 A single-tube nested PCR was designed and optimized for the detection

212 A nested PCR was developed to amplify the variable region

213 When RT-nested PCR was performed on 10-fold serial dilutions of

214 Nested PCR was performed only in the samples from vascul

215 PCR was essentially negative, a second-round nested PCR was performed, which revealed expression also

216 The nested PCR was repeated on DNA extracted from a differen

217 The nested PCR was tested for sensitivity on 20 samples from

218 Nested PCR was used to amplify exon 15 of the BRAF gene,

219 Nested PCR was used to detect different RV strains, and

220 Species-specific nested PCR was used to detect Treponema amylovorum, Trep

221 Prevalence rates obtained with nested PCR were significantly higher than those obtained

222 cted patients tested positive for gag by non-nested PCR whereas the two other assays did not detect X

223 A single-tube nested PCR which amplifies the internal transcribed spac

224 xons flanking the predicted exon, and a semi-nested PCR with a primer that targets the predicted exon

225 We used a nested PCR with Borrelia flagellin gene (flaB) primers a

226 TaqMan compared favorably with nested PCR with key advantages of speed, increased throu

227 Nested PCR with patient IgH allele-specific oligonucleot

228 tuberculosis-specific DNA was amplified in a nested PCR with previously described primers (primers rp

229 sensitive and 93.3% specific compared with a nested PCR with primer set CP1/2-CPC/D for clinical resp

230 proteinase K and tested by a single or fully nested PCR with primers directed against part of the two

231 s assay by comparing the results obtained by nested PCR with those obtained by TaqMan PCR.

232 Nested PCRs with primer sets specific for the viral T pr

233 had a diagnostic accuracy similar to that of nested PCR, with a greatly reduced time to result, and w

234 roducts were used as templates in subsequent nested PCR, with primers that amplify a 186-bp product i