ライフサイエンスコーパス: netropsin

1 DNA upon binding of the minor groove binder netropsin.

2 phenylamidines were investigated along with netropsin.

3 be blocked by the minor groove binding drug netropsin.

4 pin of sequence 5'-CGAATTCGTCTCCGAATTCG) and netropsin.

5 ercoiled form by ligation in the presence of netropsin.

6 d cytotoxicity can be inhibited in vivo with netropsin, a potent competitive inhibitor of binding of

7 However, netropsin affects neither the level of N3-methyladenine

8 ferential duplex affinity exhibited by 5PTB, netropsin and 4',6-diamidino-2-phenylindole (DAPI), two

9 between two minor groove binding compounds, netropsin and 4,6-diamidino-2-phenylindole (DAPI), and a

10 binding ligands (Hoechst 33258, distamycin, netropsin and berenil) with DNA fragments which have bee

11 ructures of the two duplexes, in contrast to netropsin and DAPI, which bind with similar affinities t

12 a series of bis-linked lexitropsins based on netropsin and distamycin have been screened for their ef

13 The minor groove binders netropsin and distamycin inhibited oxopropenylation, but

14 :1 recognition beyond the initial studies on netropsin and distamycin.

15 data have shown cooperativity of binding for netropsin and Hoechst 33258 and have provided ligand:DNA

16 eir association to the (AATT)2 binding site, netropsin and pentamidine acquire 26+/-3 and 34+/-2 addi

17 ss of compounds is related to the linked bis-netropsins and bis-distamycins, but here, only one amino

18 d that minor groove-binding ligands berenil, netropsin, and distamycin and the intercalating ligand a

19 groove binding ligands, such as distamycin, netropsin, and GLX, an indole-linked dimer of netropsin,

20 4',6-diamidino-2-phenylindole (DAPI), netropsin, and pentamidine are minor groove binders that

21 GCAAATTTGCGC)2 also forms two complexes with netropsin, and the complex with weaker affinity is again

22 Both cationic ends of netropsin are bridged by water molecules in one of the i

23 The netropsins are held by hydrogen bonding interactions to

24 ook 2 (HMGA2) as the protein of interest and netropsin as the inhibitor of HMGA2-DNA interactions.

25 eveloped: (i) two different bound species of netropsin at single binding sites and (ii) a netropsin i

26 Cooperativity of netropsin binding to a 12mer with two potential sites ha

27 The guanidinium moiety of netropsin binds in a narrow part of the minor groove, wh

28 Furthermore, the minor groove ligand netropsin binds to the minor groove and to the hydrophob

29 momycin A(3) (GC-specific), distamycin A and netropsin (both AT-specific), have been chosen.

30 the first example of a crystal structure of netropsin bound to decamer DNA.

31 The crystal structure of netropsin bound to the decamer d(CGCAATTGCG) has been de

32 elty of the structures is that there are two netropsins bound end-to-end in the minor groove of each

33 etropsin, and GLX, an indole-linked dimer of netropsin, can effectively disrupt the UL9-DNA complex o

34 , although an orientational disorder for the netropsins cannot be excluded.

35 The asymmetric unit of the RT fragment-DNA-netropsin crystals contains one protein molecule and one

36 y for several agents including distamycin A, netropsin, DAPI, Hoechst 33258, and berenil is described

37 Although netropsin, distamycin, and all other monomeric DNA binde

38 tries, AT sequence specific ligands, such as netropsin, distamycin, and GLX, prefer uniform, narrow m

39 We compare this structure to other Class I netropsin-DNA structures and find that the asymmetry of

40 In contrast to previously reported netropsin-DNA structures, our oligonucleotide contains t

41 ridging water molecule in the lower affinity netropsin/DNA complex.

42 es were in excellent agreement; for example, netropsin/DNA formation constants were determined to be

43 Netropsin, ethidium, daunorubicin and actinomycin, ligan

44 side-by-side binding mode of the distamycin/netropsin family of drugs.

45 Netropsin, for example, has two DNA binding enthalpies i

46 In buffer, netropsin forms two complexes with a net stoichiometry o

47 of the DNAs investigated clearly shows that netropsin forms two different complexes at AATT sites, a

48 With (CGCGCAATTGCGCG)2, netropsin has one binding mode in buffer, and complex fo

49 ts with minor groove binding agents, such as netropsin, have provided detailed, atomic level views of

50 andactinomycin D) and minor groove binders (netropsin, Hoechst 33258 and distamycin) has shown the s

51 netropsin at single binding sites and (ii) a netropsin induced DNA hairpin to duplex transition.

52 DNA/netropsin interactions are investigated as a function of

53 halpy and heat capacity changes suggest that netropsin interacts similarly with these two oligonucleo

54 Netropsin is a well-characterized DNA minor groove bindi

55 Netropsin is bound to the DNA decamer d(CCCCCIIIII)2, th

56 elated minor groove binders distamycin A and netropsin (K(i) </=1 microM).

57 inding reactions involving the ligands DAPI, netropsin, lexitropsin, and the lambda repressor binding

58 molecule and one-half of the 16mer with one netropsin molecule bound.

59 The netropsin molecule fits within a five base pair long min

60 The netropsin molecule lies within the minor groove of the o

61 Each netropsin molecule spans approximately 5 bp.

62 By contrast, the binding to either duplex of netropsin or DAPI induces little or no change in the ele

63 The netropsins refined with their guanidinium heads facing e

64 groove binding agents (DAPI, distamycin, and netropsin) showed a strong preference for AT-rich duplex

65 ded by this screening excise, we showed that netropsin, the specific inhibitor of HMGA2-DNA interacti

66 dynamically distinct complexes on binding of netropsin to a number of hairpin-forming DNA sequences c

67 Studies done on the binding of netropsin to similar constructs, a 24-mer and a 28-mer,

68 ng interactions from the amide groups of the netropsin to the A x T base pairs of the minor groove.

69 However, and relative to ATT, binding of netropsin to the hydrophobic groove has a decreased bind

70 Binding of netropsin to the minor groove yielded thermodynamic prof

71 The well-characterized minor groove binder netropsin was used to develop and test the assay.

72 The antibiotic compound netropsin, which undergoes electrostatic and sequence-sp

73 nucleotide contains two AATT sites that bind netropsin with flanking 5' and 3' sequences that are not

74 We have co-crystallized netropsin with the RT fragment bound to the symmetric 16

75 Thus, the interaction of netropsin with these A/T binding sites is influenced bot

76 lutes and DNA sequence on the interaction of netropsin with three duplexes has been studied by isothe

77 AATT site contributes to the orientation of netropsin within the groove while hydrogen bonding patte