ライフサイエンスコーパス: neuroligin 1

1 urexin 1beta are mixed with cells expressing neuroligin 1.

2 hat are similar in structure and sequence to neuroligin 1.

3 n, subtractive display identified YWK-II and neuroligin 1.

4 minants particular to the unique function of neuroligin 1.

5 2 ligands, again rendering LRRTM2 similar to neuroligin-1.

6 synapses in transfected neurons similarly to neuroligin-1.

7 Neuroligins 1, 2, and 3 were active in this assay.

8 We identified chicken orthologs of neuroligins 1, -3, and -4, but could find no evidence of

9 Whereas neuroligins-1, -3, and -4 localize to glutamate postsyna

10 ces the ability of neurexin-1beta to cluster neuroligin-1/3/4 and glutamatergic postsynaptic proteins

11 Neuroligins 1-4 are postsynaptic transmembrane proteins

12 1 exhibit enhanced spinal cord expression of neuroligin 1, a cell-adhesion postsynaptic protein regul

13 rons and cocultured tsA 201 cells expressing neuroligin-1, a postsynaptic binding partner of neurexin

14 Beta-neurexins are candidate receptors for neuroligin-1, a postsynaptic membrane protein that can t

15 CL1 competed for neurexin binding with neuroligin-1, a well characterized neurexin ligand.

16 Mutations in two surface loops of neuroligin 1 abolished neuroligin binding to neurexin 1b

17 downstream of mTOR effects the expression of neuroligin 1 and excitatory synaptic transmission in the

18 in (GABARAP; approximately 13%), but not for neuroligin 1 and gephyrin.

19 generated transfected cell lines expressing neuroligin 1 and neurexin 1beta.

20 excitatory and inhibitory synaptic proteins neuroligin 1 and neuroligin 2, which promote memory stre

21 These subtypes, neuroligin 1 and neuroligin 3, have roles in synaptogene

22 r splice insertions restrict the function of neuroligin-1 and -2 to glutamatergic and GABAergic conta

23 sing an in vitro system, we demonstrate that neuroligin-1 and -2, postsynaptically localized proteins

24 that is lacking in fragile X syndrome, binds neuroligin-1 and -3 mRNA.

25 g to neurexin-1 beta, but not the folding of neuroligin-1 and confirm the validity of the binding int

26 g, whereas wild-type streptavidin aggregated neuroligin-1 and disrupted presynaptic contacts.

27 in-1beta forms a trans-synaptic complex with neuroligin-1 and neuroligin-2 and that this interaction

28 Here, we generated neurexin-1beta and neuroligin-1 and neuroligin-2 fusion proteins containing

29 y, we found a functional distinction between neuroligins 1 and 3.

30 es the binding affinity of neurexin-1beta to neuroligins-1 and -4 but has little effect on binding to

31 We conclude that neurexin 1beta and neuroligin 1 (and, by extension, other beta-neurexins an

32 of identified PDZ3-binding proteins (CRIPT, neuroligin-1, and citron) and (2) selective mutation of

33 urexins, blocks the synaptogenic activity of neuroligin-1, and reduces the density of presynaptic ter

34 vely spliced sites in neurexin-1 beta and in neuroligin-1 are positioned nearby the binding interface

35 The extracellular domain of functional neuroligin-1 associates as a dimer when analyzed by sedi

36 utes to the specificity of the neurexin-beta/neuroligin-1 association.

37 Structure-based mutations of neuroligin-1 at the interface disrupt binding to neurexi

38 in 1 is regulated by alternative splicing of neuroligin 1 (at splice site B) and of neurexins (at spl

39 asmon resonance, we established that soluble neuroligins-1 bind neurexin-1beta, but the homologous al

40 free Ca2+, which probably acts by binding to neuroligin 1 but not to neurexin 1beta.

41 To determine if neurexin 1beta and neuroligin 1 can also interact with each other when pres

42 extremely long genes, including Neurexin-1, Neuroligin-1, Cntnap2, and GABA(A)beta3.

43 as exemplified by miR-146a, which inhibited neuroligin 1-dependent synaptogenesis.

44 ntical surfaces on the opposite faces of the neuroligin-1 dimer to form a heterotetramer.

45 Moreover, neuroligin-1 dimerization was not required for either th

46 evertheless, both alpha-neurexin binding and neuroligin-1 dimerization were essential for the increas

47 alpha- and beta-neurexin binding, or abolish neuroligin-1 dimerization.

48 ides chain or the terminal sialic acids from neuroligin-1 enhance its activity, whereas deglycosylati

49 ivity was selective for neuroligin-2 and not neuroligin-1 excitatory synapse organizer.

50 Neuroligin-1 forms a constitutive dimer, and two neurexi

51 pressed five soluble and exportable forms of neuroligin-1 from recombinant DNA sources, by truncating

52 Neuroligin-1 has a unique N-linked glycosylation pattern

53 d from split-GFP-modified neurexin-1beta and neuroligin-1 if and after neurexin-1beta bound to its ne

54 identify a subtype-specific role in LTP for neuroligin 1 in young CA1, which persists into adulthood

55 overexpression of AMPA receptors along with Neuroligin-1 in 293T cells is sufficient to stabilize pr

56 Overexpression of neuroligin-1 in control or neuroligin-deficient neurons

57 Expression of a dominant-negative neuroligin-1 in cultured neurons markedly reduced the si

58 Here, we present the crystal structures of neuroligin-1 in isolation and in complex with neurexin-1

59 Accordingly, deletion of neuroligin-1 in knockout mice selectively decreases the

60 in apparent synapse size that is induced by neuroligin-1 in transfected neurons.

61 Conversely, the overexpression of neuroligin-1 increased synapse numbers but not spine num

62 e deacetylase (HDAC) inhibitors that improve neuroligin-1-induced synaptogenesis by modulating class-

63 Thus, in this assay, neuroligin-1 induces apparent synapse formation by bindi

64 cis-expression of neurexin-1beta with neuroligin-1 inhibits trans-binding to recombinant neure

65 red in these neurons, confirming that PSD-95/neuroligin-1 interaction is involved in postsynaptic ass

66 Neuroligin 1 is a neuronal cell surface protein that bin

67 and that alpha- and beta-neurexin binding by neuroligin 1 is regulated by alternative splicing of neu

68 overexpression of neuroligin-3, which, like neuroligin-1 is also targeted to excitatory synapses, ha

69 inhibition of eIF4E or genetic reduction of neuroligin 1 levels normalizes the increased excitatory

70 detected in the inner retina, low levels of neuroligin 1 mRNA were also detected in the photorecepto

71 hesis, we examined the functional effects of neuroligin-1 mutations that impair only alpha-neurexin b

72 The neuroligin-1/neurexin-1 beta complex exhibits a nanomola

73 the validity of the binding interface of the neuroligin-1/neurexin-1 beta complex.

74 We deleted neuroligin-1, neuroligin-2, and neuroligin-3, the major

75 The localization and synaptic effects of neuroligin-1 (NL-1, also called NLGN1) are specific to e

76 cently, the synaptic cell adhesion molecules neuroligin 1 (NL1) and SynCAM were shown to induce presy

77 eta-neurexin association, splice insert B in neuroligin-1 (NL1) is the key element regulating the NL1

78 The cell adhesion molecule neuroligin-1 (NL1) modifies NMDAR-dependent synaptic tra

79 We found that neuroligin-1 (NL1), which is located at excitatory posts

80 Syt4 and the transsynaptic adhesion protein Neuroligin 1 (Nlg1).

81 synaptic adhesion molecules neurexin-1beta, neuroligin-1 (Nlg1) and leucine-rich-repeat transmembran

82 The synaptic adhesion molecule Neuroligin-1 (NLG1) controls the activity and synaptic l

83 tivity induces acute proteolytic cleavage of neuroligin-1 (NLG1), a postsynaptic adhesion molecule at

84 2 (NLGN2) limited to inhibitory synapses and neuroligin 1 (NLGN1) dominating at excitatory synapses.

85 ne showed some evidence for association near neuroligin 1 (NLGN1) on chromosome 3, but did not suppor

86 The synaptic protein Neuroligin 1 (NLGN1), a cell adhesion molecule, is criti

87 Neuroligin 1 (NLGN1), a postsynaptic protein found in ce

88 ed heterologous synapse formation induced by neuroligin-1 or LRRTM2.

89 ioned such that binding of neurexin-1beta to neuroligin-1 or neuroligin-2 allowed GFP reconstitution

90 We now show that in cultured neurons, neuroligin-1 overexpression increases excitatory, but no

91 Thus, neuroligin-1 performs diverse synaptic functions by mech

92 In this issue, Liu et al. demonstrate that neuroligin 1 promotes actin assembly associated with syn

93 At retinal synapses neuroligin 1 protein was detected in the inner plexiform

94 Moreover, neuroligin 1 splice variants with distinct neurexin bind

95 In neuroligin 1, splice site B is a master switch that dete

96 ties differentially regulate synaptogenesis: neuroligin 1 that binds only beta-neurexins potently sti

97 tently stimulates synapse formation, whereas neuroligin 1 that binds to both alpha- and beta-neurexin

98 gated the interaction of neurexin 1beta with neuroligin 1 to evaluate their potential to function as

99 as essential for the ability of postsynaptic neuroligin-1 to dramatically increase synapse density, s

100 mice targeting all three major neuroligins [neuroligin-1 to neuroligin-3 (NL123)] with parvalbumin-C

101 with monovalent streptavidin allowed stable neuroligin-1 tracking without cross-linking, whereas wil

102 , the AMPA-type glutamate receptor GluA1 and neuroligin 1 undergo spatially restricted entry into the

103 suppresses the synapse-boosting activity of neuroligin-1, whereas chronic inhibition of general syna

104 eurexins are decreased, whereas the level of neuroligin 1 (which binds to neurexins that in turn bind

105 Labeling of site-specifically biotinylated neuroligin-1 with monovalent streptavidin allowed stable