ライフサイエンスコーパス: nick

1 sponse of supercoiled DNA to a single strand nick.

2 laying a specific role in bending DNA at the nick.

3 sis but are rescued by overexpression of Myc-nick.

4 ivalent configurations on the 3' side of the nick.

5 he relative strandedness of the ODNs and the nick.

6 nucleoside-2',3'-cyclic phosphate-terminated nicks.

7 t the nick sites, mimicking the Top1-induced nicks.

8 tion amplification followed by site-specific nicking.

9 w EcoLigA requires proper positioning of the nick 3' nucleoside for catalysis of 5' adenylylation; an

10 When the substrate contained a nick 3' to the mispair, a mixture of Msh2-Msh6 (or Msh2-

11 onuclease in the presence of a mispair and a nick 3' to the mispair, to make nicks 5' to the mispair,

12 n double-stranded nucleic acids in which the nick 3'-OH end is RNA.

13 DNA intermediate (step 2); and attack of the nick 3'-OH on AppDNA to form a 3'-5' phosphodiester (ste

14 ntermediate (step 1); transfer of AMP to the nick 5'-PO4 to form an AppDNA intermediate (step 2); and

15 ispair and a nick 3' to the mispair, to make nicks 5' to the mispair, allowing Exo1 to excise the mis

16 he wild-type enzyme N.varphiGamma prefers to nick 5mCG-modified DNA in Ni2+ buffer even though the ni

17 generated fusion enzymes that preferentially nick 5mCG-modified DNA.

18 This generates Myc-nick, a cytoplasmic, transcriptionally inactive cleavage

19 synthesis and ligand-dependent TOP1-mediated nicking-a strategy exerting quantitative effects on eRNA

20 nalysis indicates that PARP1 binds to DNA at nicks, abasic (AP) sites, and ends as a monomer.

21 MYC-nick accelerates migration by activating the Rho GTPase

22 ntal evidence, we propose a model where RepC nicking activity is passive and dependent upon the super

23 -modified DNA in Ni2+ buffer even though the nicking activity is sub-optimal compared to the activity

24 Furthermore, we show that the DNA nicking activity of TOP1 is a prerequisite for robust eR

25 e first time, we show Rep protein off-target nicking activity, highlighting the importance of the nic

26 DNA ends at the IgH locus on chromosome 14, nicks AID-generated TG mismatches at methyl CpG sites, a

27 Myc-nick also delays colon cancer cell death after treatment

28 cancer translocations induced by paired Cas9 nicks also showed a dependence on c-NHEJ, despite having

29 reover, it performed primary "polymerization-nicking" amplification and mediate secondary HRCA.

30 We find that GEN1 cleaves HJs by a nick and counter-nick mechanism involving dual co-ordina

31 n duplex DNA as demonstrated with a backbone nick and extrahelical bulge.

32 The fusion enzyme can be used to nick and label 5mCG-modified plasmid and genomic DNAs wi

33 nderstanding of molecular mechanisms driving nick and paired-nick repair in mammalian cells and clari

34 second Top1 cleavage complex adjacent to the nick and subsequent faulty Top1 religation led to the sh

35 mplementarity that promotes realignment to a nick and subsequent Top1-mediated ligation.

36 replication origin, which contains both NS1 nicking and binding sites, to a 46-nucleotide sequence i

37 , the physiologically relevant metal ion, in nicking and double-strand cleavage of both RSS DNAs to p

38 Following nicking and powered by ATP hydrolysis, the concerted act

39 agnetic tweezers to directly measure the DNA nicking and religation activities of RepC, the replicati

40 f this cruciform and the implication for its nicking and religation functions is unclear.

41 Top1 relieves torsional stress by nicking and resealing one DNA strand, and some Top1-depe

42 gnal sequence (RSS)-conserved regions before nicking and synapsis.

43 ise from DNA regions expected to undergo DNA nicking and/or double-strand breaks.

44 Here we report that (-)-lomaiviticin A nicks and cleaves plasmid DNA by a pathway that is indep

45 Exo1 processing at DNA nicks and double-strand breaks creates long stretches of

46 simplex virus 1 (HSV-1) virion DNA contains nicks and gaps, and in this study a novel assay for esti

47 Even single-strand nicks and targeted biochemical modifications that do not

48 methylated d(GATC) site or a single-stranded nick) and the replication error after the error is ident

49 tivities of Rep68, including DNA binding and nicking, and compromises viral DNA replication and trans

50 ochemical activities, including DNA binding, nicking, and unwinding.

51 hat residues responsible for NS1 binding and nicking are within the origin-binding domain.

52 In human cells, nicks are efficiently repaired via the single-strand bre

53 onsistent with a DSB intermediate, even when nicks are induced up to approximately 1kb apart.

54 ning both spontaneous and DNA damage-induced nicks are prone to breakage during PFGE.

55 activate repair: D10A but not N863A-induced nicks are repaired by homologous recombination.

56 DNA nicks are the most common form of DNA damage, and if unr

57 es containing single-stranded DNA regions or nicks as well as relax negatively supercoiled DNA.

58 RNaseH2 must be efficient at recognizing and nicking at embedded ribonucleotides to ensure genome int

59 he opposite strand from the Top1-induced DNA nicks at ribonucleotide sites.

60 ions implicated processing of Top1-generated nicks at rNMP sites and/or sequential Top1 binding, but

61 ized CRISPR/Cas tools can be used for offset nicking-based mutagenesis.

62 ctivity of topoisomerase I (Top1) causes DNA nicks bearing 2',3'-cyclic phosphates at ribonucleotide

63 Relative to a single nick, nicking both strands enhances HR, consistent with a DSB

64 single-strand rather than double-strand DNA nicks/breaks.

65 HDR at nicks, but not DSBs, is associated with transcription an

66 and NHEJ compete for repair of these paired nicks, but, surprisingly, only when 5' overhangs or blun

67 Nicking by RepC occurred only in negatively supercoiled

68 de strand, and Cas9 nickases used for paired nicking can also tolerate bulges in one of the guide str

69 HDR at nicks can proceed by a pathway dependent upon canonical

70 on by peroxyl and hydroxyl radicals into the nicked circular form was also investigated.

71 Paradoxically, the MMR-nicking complex Pms2/Mlh1 is apparently dispensable for

72 We used the double-nicking CRISPR/Cas9 system to conduct site-specific muta

73 primers released from above "polymerization-nicking" cycles were separated out to trigger the subseq

74 Similarly, single nicks deriving from different Cas9 variants differential

75 econstitution of an Mlh1-Pms1-independent 5' nick-directed mismatch repair (MMR) reaction using Sacch

76 catalyze both short-patch and long-patch 5' nick-directed MMR of a substrate containing a +1 (+T) mi

77 ) and a reconstituted Mlh1-Pms1-dependent 3' nick-directed MMR reaction requiring Msh2-Msh6 (or Msh2-

78 fects induced by pairs of Cas9 variants that nick DNA (paired nickases).

79 orm an active nucleoprotein complex that can nick DNA substrates in trans.

80 d is activated by mononucleosomes containing nicked DNA and which target PARP3 trans-ribosylation act

81 ity of Arabidopsis cell extracts to ligate a nicked DNA intermediate.

82 We discuss how breakage of nicked DNA may be mechanistically linked to trapping.

83 e cleavage intermediate is mostly top-strand nicked DNA on a single-site plasmid.

84 pens after catalysis, leading to a cytotoxic nicked DNA repair intermediate.

85 Paradoxically, when DNA ligases encounter nicked DNA structures with abnormal DNA termini, DNA lig

86 rt deletions at the rNMP sites by generating nicked DNA substrates bearing 2',3'-cyclic phosphates at

87 Moreover, when acting on the nicked DNA substrates containing 2',3'-cyclic phosphates

88 entially the coding DNA strand, generating a nicked DNA target.

89 n times more efficient than LIG1 at ligating nicked DNA under optimal conditions, mainly because of t

90 g double-stranded DNA after filling a gap or nicked DNA.

91 nits covalently linked to the 5' ends of the nicked DNA.

92 e is reduced by inactivating RNase H2, which nicks DNA containing ribonucleotides incorporated during

93 tion determined by crystallography, where it nicks DNA exiting from RecC and loads RecA onto the newl

94 cal HJ resolvase, functions as homodimer and nicks DNA strands precisely across the junction point.

95 ing domain (OBD) that specifically binds and nicks DNA.

96 The small modular HNH nicking domain can be used to generate rare NEases appli

97 The nicking domains (NDs) of E59A and 11 double mutants were

98 e replication tracks that yield the Nt.BbvCI nicking domains and the respective Mg(2+)-dependent DNAz

99 genes and replication tracks that yield the nicking domains for Nt.BbvCI and two different Mg(2+)-de

100 The final produced long nicked double-stranded DNA loses the ability to protect

101 RNA ligases were not able to modify a 3'p in nicked double-stranded DNA.

102 hat recombinant MutLgamma is a nuclease that nicks double-stranded DNA.

103 At 5' nucleobase-abasic site nicks, DraRnl prefers to ligate when the nucleobase is a

104 lternative pathway that uses either ssDNA or nicked dsDNA donors and that is strongly inhibited by RA

105 ecule level using braids of intact dsDNA and nicked dsDNA with bulges.

106 DNA breaks are generated by multiple random nicks due to mobility of a collision complex with an ove

107 On a nicked duplex DNA substrate, the results reveal binding

108 ions that lead to the formation of ligatable nicked duplex products.

109 dehyde residue embedded within the resulting nicked duplex.

110 of terminal deoxynucleotidyltransferase dUTP nick end label (TUNEL)-positive macrophages in the lymph

111 Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) and phospho-histone H3 (PH3) s

112 y terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) and used to estimate the occur

113 ynucleotidyltransferase-mediated dUTP-biotin nick end labeling (TUNEL) assays, and transmission elect

114 judged by lack of terminal transferase dUTP nick end labeling (TUNEL) labeling or reactivity to anti

115 y terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) staining in situ.

116 ynucleotidyltransferase-mediated dUTP-biotin nick end labeling (TUNEL) staining of intestinal section

117 l deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) staining, and Western blot for

118 , terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL), 3-(4,5-dimethylthiazol-2-yl)-

119 ynucleotidyltransferase-mediated dUTP-biotin nick end labeling (TUNEL)-positive cells in several regi

120 h terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL)-positive soma and the eventual

121 (terminal deoxynucleotidyl transferase dUTP nick end labeling [TUNEL] assay), and of collagen type I

122 (terminal deoxynucleotidyl transferase dUTP nick end labeling and Annexin V assays).

123 nucleotidyl transferase-mediated dUTP-biotin nick end labeling assay and Hoechst staining.

124 d terminal deoxynucleotidyl transferase dUTP nick end labeling assay was used to assess alveolar cell

125 a terminal deoxynucleotidyl transferase dUTP nick end labeling staining and caspase-3 activation.

126 ynucleotidyltransferase-mediated dUTP-biotin nick end labeling) assay determined that contact with th

127 (terminal deoxyneucleotidyl transferase dUTP nick end labeling) expression in xenografts.

128 al deoxynucleotidyltransferase-mediated dUTP nick end labeling, caspase 3 cleavage, and re-localizati

129 l deoxynucleotidyl transferase-mediated dUTP nick end labeling-positive apoptotic cells (8.3% +/- 1.4

130 [terminal deoxynucleotidyl transferase dUTP nick end labeling])-positive cells) of NPIs compared wit

131 se-mediated deoxyuridine triphosphate-biotin nick end labelling (TUNEL), was performed to confirm the

132 rough the terminal dUTP transferase-mediated nick end-labeling (TUNEL) assay followed by counting the

133 ansferase-mediated deoxyuridine triphosphate nick end-labeling (TUNEL)-positive cells and mortality c

134 l deoxynucleotidyl transferase-mediated dUTP nick end-labeling staining.

135 ansferase-mediated deoxyuridine triphosphate nick end-labeling, and caspase activation.

136 minal deoxyribosyl transferase-mediated dUTP nick-end labeling (TUNEL) assay, and real-time RT-PCR.

137 al deoxynucleotide transferase-mediated dUTP nick-end labeling (TUNEL) assay; (2) frequencies of Th s

138 ansferase-mediated deoxyuridine triphosphate nick-end labeling (TUNEL) histology.

139 y terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) staining, circulating levels o

140 ion and DNA fragmentation (TdT-mediated dUTP nick-end labeling [TUNEL]).

141 d markers including TdT-mediated dUTP biotin nick-end labeling and cleaved caspase 3 immunofluorescen

142 l deoxynucleotidyl transferase-mediated dUTP nick-end labeling assay was performed, and intestinal in

143 Terminal deoxynucleotidyl transferase dUTP nick-end labeling staining was used for the identificati

144 d terminal deoxynucleotidyl transferase dUTP nick-end labeling staining were tested for apoptosis.

145 serum transaminases, bilirubin, triphosphate nick-end labeling staining, caspase-3 activity, oxidativ

146 l deoxynucleotidyl transferase-mediated dUTP nick-end labeling staining.

147 l deoxynucleotidyl transferase-mediated dUTP nick-end labeling).

148 l deoxynucleotidyl transferase-mediated dUTP nick-end labeling, and Ki-67 immunoreactivity were evalu

149 y terminal deoxynucleotidyl transferase dUTP nick-end labeling, was significantly decreased in the pr

150 d terminal deoxynucleotidyl transferase dUTP nick-end labeling-positive cells.

151 ansferase-mediated deoxyuridine triphosphate nick-end labeling-positive nuclei and accumulation of cy

152 to initiate the polymerization extension and nicking endonuclease cleavage reaction.

153 ee-arm DNA junction, subsequently initiating nicking endonuclease-assisted isothermal fluorescence si

154 HNHEs are site-specific and strand-specific nicking endonucleases (NEase or nickase) with 3- to 7-bp

155 s observe the response of supercoiled DNA to nicking endonucleases and topoisomerases.

156 ases, RNase H2 can be replaced with specific nicking endonucleases in this protocol; we term this met

157 TR include sequence-dependent restriction or nicking endonucleases or sequence independent exonucleas

158 a is a strand-specific and site-specific DNA nicking enzyme (YCG downward arrowGT or AC upward arrowC

159 Here, we describe NicE-seq (nicking enzyme assisted sequencing) for high-resolution

160 rporates a G-quadruplex structure flanked by nicking enzyme recognition sites.

161 Cas9 can also be converted into a nicking enzyme to facilitate homology-directed repair wi

162 io-bar-code amplification (BCA) and Nb.BbvCI nicking enzyme-assisted strand cycle for exponential sig

163 serted at user-defined sites via an improved nicking enzyme-based strategy.

164 tivities of Bst 2.0 polymerase and Nt.BstNBI nicking enzyme.

165 MYC-nick, fascin, and Cdc42 are frequently up-regulated in c

166 s cleaved by Fen1, and DNA ligase sealed the nick for complete repair.

167 protein shows specificity for binding to and nicking forked DNA within single strand gaps, and collap

168 winding individual, torsionally constrained, nick-free dsDNA molecules, we measured the contour lengt

169 nded DNA with structural features, including nicks, gaps, 5'-flaps, Kappa joints, synthetic replicati

170 e H2, Srs2 unwinds DNA from the 5' side of a nick generated by DNA topoisomerase I at a ribonucleosid

171 Nicks generated by either I-AniI or the CRISPR/Cas9(D10A

172 ecombination (HR), but single-strand breaks (nicks) have also been shown to trigger HR.

173 te of that observed with otherwise identical nicked heteroduplex DNA.

174 itions observed for the resolution of mobile nicked HJs suggest that these cleavage positions are det

175 rther reveal that recognition of 3'-flap and nicked Holliday junction substrates by Mus81-Mms4 involv

176 EME2 cleaves 3'-flaps, replication forks and nicked Holliday junctions, and exhibits limited endonucl

177 wnstream duplex, such as fork structures and nicked Holliday junctions.

178 However, the mechanisms of (nick)HR are largely unexplored.

179 Here, we applied Cas9 nickases to study (nick)HR in mammalian cells.

180 We find that (nick)HR is unaffected by inhibition of major damage sign

181 Both DSB- and nick-induced HR ((nick)HR) are exploited in advanced genome-engineering ap

182 We found conversion of Myc into Myc-nick in cell lines and tissues derived from multiple can

183 ied recombination sites with a single-strand nick in one of the two DNA strands.

184 of Cas9, to create a guide RNA-directed DNA nick in the context of an in vitro-assembled CRISPR-CAS9

185 However, an additional nick in the donor plasmid backbone markedly improved the

186 -established significance of strand-specific nicking in MMR, the mechanism(s) by which MutS and MutL

187 ease but forms polymers required to generate nicks in DNA.

188 NA ligase and RtcA can use 3'-phosphorylated nicks in double-stranded DNA to produce a 3'-adenylated

189 DraRnl ligates 3'-OH, 5'-PO4 nicks in double-stranded nucleic acids in which the nick

190 li DNA ligase (EcoLigA) repairs 3'-OH/5'-PO4 nicks in duplex DNA via reaction of LigA with NAD(+) to

191 odurans RNA ligase (DraRnl) seals 3-OH/5-PO4 nicks in duplex nucleic acids in which the 3-OH nick ter

192 naked DNA stimulate PARP3 autoribosylation, nicks in mononucleosomes promote the trans-ribosylation

193 Although nicks in naked DNA stimulate PARP3 autoribosylation, nic

194 e ligase IIIalpha-XRCC1 complex binds to DNA nicks in nucleosomes only when they are exposed by perio

195 Topoisomerase 1 (TOP1) generates transient nicks in the DNA to relieve torsional stress encountered

196 We show that a combination of single nicks in the target gene and donor plasmid (SNGD) using

197 Both DSB- and nick-induced HR ((nick)HR) are exploited in advanced gen

198 Interestingly, single-nick-induced PGE using ODN donors produced conversion tr

199 Our results suggest that Myc-nick-induced survival and motility contribute to colon c

200 Myc-nick-induced survival, autophagy, and motility require M

201 MYC-nick is a cytoplasmic, transcriptionally inactive member

202 Here we report that MYC-nick is abundant in colonic and intestinal tumors derive

203 wever, the 2',3'-cyclic phosphate-terminated nick is also processed by Top1 incision, generally 2 nuc

204 Moreover, MYC-nick is elevated in colon cancer cells deleted for FBWX7

205 In colon cancer, the production of Myc-nick is enhanced under stress conditions such as hypoxia

206 Remarkably, this nick is rapidly reverted by Top1, thereby providing anot

207 We propose the top-strand nicking is carried out by a Tth111II monomer and bottom-

208 Nicking is performed by a replication-initiation protein

209 Recruitment and nicking is stimulated by the presence, but not hydrolysi

210 s substrates tailor its incision activity to nicked junction molecules.

211 ates with correctly base-paired 3'-OH/5'-PO4 nicks, kstep2 was fast (6.8-27 s(-1)) and similar to kst

212 ts in joining intermolecular DNA ends versus nick ligation is unclear.

213 ndary coupled activation of a polymerization/nicking machinery and DNAzyme generation path leads to a

214 Here, we observed that venous nicking may be observed in the absence of physical conta

215 hese results suggest that alternative HDR at nicks may be stimulated in physiological contexts in whi

216 that GEN1 cleaves HJs by a nick and counter-nick mechanism involving dual co-ordinated incisions tha

217 Here we present nicking mutagenesis, a robust, single-day, one-pot satur

218 us segments showed a variable association of nicking, narrowing, deviation, and opacification.

219 only leading strand synthesis starting at a nick near one covalently closed end of the genome and co

220 to generate two unhooked DNA duplexes with a nick, NEIL3 targets both DNA strands in the ICL without

221 Relative to a single nick, nicking both strands enhances HR, consistent with

222 relatively little is known about the fate of nicks not processed by that pathway.

223 data identify PARP3 as a molecular sensor of nicked nucleosomes and demonstrate, for the first time,

224 ncisions can be uncoupled and that the first nick occurs upon GEN1 dimerization at the junction.

225 show that homology-directed repair (HDR) at nicks occurs via a mechanism distinct from HDR at double

226 Unusually, concentration-dependent nicking of duplex DNA appeared to require only transient

227 ORF1p depletion and reduced the L1-mediated nicking of genomic DNA.

228 equence-specific recognition for initiation, nicking of one of the template DNA strands and unwinding

229 ase domain in Chi recognition, indicate that nicking of one strand at Chi is RecBCD's biologically im

230 sh2-Msh3), PCNA, and RFC but did not require nicking of the substrate, followed by a second stage in

231 t a nick on the transcribed strand than at a nick on the nontranscribed strand.

232 ription and is eightfold more efficient at a nick on the transcribed strand than at a nick on the non

233 re demonstrated via the marked effect of DNA nicking on histone eviction that underscores the powerfu

234 nsfer depends on key catalytic components to nick one strand of the duplex DNA plasmid and separate t

235 requires a 5'-terminal phosphate anchor at a nick or a 1- or 2-nucleotide flap and is augmented by a

236 ir of sequence-specific nuclease-induced DNA nicking or double-strand breaks (DSBs) by homology-direc

237 eosomes containing discretely positioned DNA nicks, our evidence indicates that the ligase IIIalpha-X

238 e (Lig) molecules searching for DNA gaps and nicks, performing transient reactions, and releasing the

239 espective recognition sequences triggers the nicking/polymerization machineries, leading to the synth

240 We identify the tile nick position as a structural requirement for lattice fo

241 lly converting ProT into active sigmaPre2, a nicked Pre2 derivative with a single cleaved Ala-470-Asn

242 -triggered isothermal autonomous replication/nicking process on the modified template results in the

243 ger on the autonomous isothermal replication/nicking processes and the displacement of a Mg(2+)-depen

244 end-joining (NHEJ) components, arguing that nick processing does not require a DSB intermediate to t

245 Srs2-Exo1 thus functions in a new pathway of nick processing-gap filling that mediates tolerance of r

246 ation forks that collapse at single-stranded nicks, producing ends that instigate genomic instability

247 these conditions, ectopic expression of Myc-nick promotes anchorage-independent growth and cell surv

248 MYC-nick promotes migration and survival of cells in respons

249 MYC-nick promotes the migration of colon cancer cells assaye

250 This nick provides an entry point for downstream repair prote

251 imated to be much slower than the top-strand nicking rate.

252 unctional magnetic probes and polymerization nicking reactions mediated hyperbranched rolling circle

253 les, as it contains both single-stranded DNA-nicking relaxase and ATP-dependent helicase domains with

254 op1 cleavage events and for the promotion of nick religation at rNMP sites by Top1.

255 molecular mechanisms driving nick and paired-nick repair in mammalian cells and clarify phenomena ass

256 pendent DNAzyme sequences are implemented as nicking/replication machineries for the amplified, multi

257 matches is translated into the single-strand nick required for error-prone synthesis is an open quest

258 5mCG-dependent nicking requires special digestion conditions in high sa

259 t a scenario in which DraRnl acts at genomic nicks resulting from gap-filling by a ribonucleotide-inc

260 We also found that ectopic expression of Myc-nick results in the induction of the actin-bundling prot

261 a catalytically dead mutant highlights that nick sealing activity is important for the radioprotecti

262 yze the kinetic mechanism of single-turnover nick sealing by EcoLigA-AMP.

263 airs and damaged base lesions on the rate of nick sealing.

264 ligase (NgrRnl) exemplifies a family of RNA nick-sealing enzymes found in bacteria, viruses, and euk

265 mplifies a widely distributed Rnl5 family of nick-sealing RNA ligases, the physiological functions of

266 Both single gRNA/WT hCas9 and double nicking set-ups were effective.

267 heteroduplex structures and is even able to nick single-base mismatches.

268 posed that the inverted DNA sequences at the nick site form a cruciform structure that facilitates DN

269 e of a DNA hairpin structure adjacent to the nick site.

270 otides are then incorporated adjacent to the nicking site with a DNA polymerase to label the guide RN

271 Chemical modifications of a nicked-site substrate at the positions of the scissile p

272 After cleavage of a nicked-site substrate, the half-site that is not covalen

273 switch modulates cleavage susceptibility of nick sites by altering both the thermodynamics and kinet

274 creatitis by altering cleavage of regulatory nick sites by chymotrypsin C (CTRC) resulting in reduced

275 rates bearing 2',3'-cyclic phosphates at the nick sites, mimicking the Top1-induced nicks.

276 al role for RNaseH2 in protecting or marking nicked sites for further processing.

277 lyses confirmed the putative NS1 binding and nicking sites within the OriR.

278 We found that Artemis is capable of nicking small heteroduplex structures and is even able t

279 arget cleavage, we further describe a double-nicking strategy using the Cas9 nickase mutant with pair

280 Recently a "double-nicking" strategy using catalytic mutant Cas9(D10A) nick

281 Although breakage of nicked subchromosomal fragments is field strength indepe

282 activity, highlighting the importance of the nicking substrate for Rep-mediated integration.

283 most bacteria, which lack a methyl-directed nicking system.

284 ks in duplex nucleic acids in which the 3-OH nick terminus consists of two or more ribonucleotides.

285 y require Myc box II (MBII), a region of Myc-nick that recruits acetyltransferases that in turn modif

286 lycosylases possess AP lyase activities that nick the DNA strand at the deoxyribose moiety via a beta

287 NA) activate the latent MutL endonuclease to nick the error-containing daughter strand.

288 -esterase activity, which is responsible for nicking the DNA strand to be transferred and for covalen

289 Nicking the target gene alone did not facilitate efficie

290 At the site of the nick there is a covalent linkage of TOP1 with DNA via a

291 -dependent enhancer activation, based on DNA nicking to relieve torsional stress from eRNA synthesis.

292 ates with RNA and LigC ligates the resulting nicks to complete repair.

293 esis of a new DNA strand and ligation of the nicks to restore the sequence integrity.

294 Un-nicked torsionally relaxed DNA is a poor substrate for t

295 rgets for the capture when the target DNA is nicked two nucleotides apart from the TA.

296 d, topo I, and increased topo I-mediated DNA nicking under conditions of oxidative stress.

297 ed that some portion of virion proteins are "nicked" via a combination of endoproteolysis and concert

298 flap endonuclease 1 (Fen1) and the resultant nick was ligated by DNA ligase to form a mature lagging

299 ion, generally 2 nucleotides upstream of the nick, which produces a covalent Top1-DNA complex with a

300 ndonucleases to cleave substrates containing nicks within their recognition sites.