ライフサイエンスコーパス: nicked

1 fraction of the DNA with multiple sites was nicked.

2 ed nicks, TREX1 removes nucleotides from the nicked 3' end to reduce the possibility of repair by rej

3 oisomerase IIalpha (TOP2A) bound to a doubly nicked, 30-bp duplex oligonucleotide.

4 se I (residues 233 to 919) in complex with a nicked, 5' adenylated DNA intermediate.

5 duced translocation efficiency, but a singly nicked 500-bp molecule was packaged as effectively as an

6 king of the first strand, a substrate with a nicked abasic site on the contralateral strand was an ev

7 model BER intermediates containing 5'- or 3'-nicked abasic sites or deoxyribosephosphate flaps were s

8 A with a particular preference for targeting nicked and bent DNA.

9 ivity and endonuclease activity that cleaves nicked and branched structures.

10 opoisomerase, resulting in polypurine strand-nicked and catenated DNA conformations.

11 Both nicked and flapped DNA substrates with photolesions (the

12 degrees of strand displacement synthesis on nicked and gapped duplex DNA templates with the relative

13 These include the nicked and hairpin products, as well as likely base unpa

14 The kinetics of nicked and linear DNA formation are comparable, both bei

15 rolyzes Litmus 29 plasmid DNA to afford both nicked and linear DNA.

16 In addition, FEN1 cleavage on both nicked and nicked-THF flap DNA resulted in a one-nucleot

17 ns are composed of one A subunit that can be nicked and reduced to an enzymatically active A1(approxi

18 anded and blunt-ended duplex substrates over nicked and tailed duplex substrate conformations.

19 in cis, thereby allowing the hairpins to be nicked and then to undergo processing and joining by non

20 ee isoforms, the linear, open-circular (oc, "nicked"), and covalently closed circular (ccc, "supercoi

21 ysis of the relative amounts of supercoiled, nicked, and linear DNA present show that there is one do

22 The condensation behavior of nicked- and gapped-DNA duplexes was investigated using s

23 and polyethylenimine, form condensates with nicked- and gapped-DNA that are significantly smaller th

24 er heating and annealing, heteroduplexes are nicked at mismatched sites by the endonuclease CEL I and

25 supercoiled plasmid pT181 DNA that had been nicked at the origin by RepC.

26 eplication (terminal repeats [TRs]) that are nicked at the terminal resolution site (trs) by the AAV

27 Cleavage-generated hairpins are nicked at the tip and predominantly 1 to 2 nt 5' of the

28 perature of 37 degrees C the reduced form of nicked BoNT/E adopts a dynamically flexible conformation

29 In contrast, Hox11 and SCL are nicked but not cleaved efficiently in vitro, and cleavag

30 atively supercoiled substrate into primarily nicked, but also linear, DNA at enzyme/DNA molar ratios

31 conversion to glycerol suggests that DNA is nicked by a free radical mechanism.

32 from which the concatemers were prepared was nicked by AP endo in a fashion similar to that of the pr

33 cosylase UNG; the resulting abasic sites are nicked by apurinic/apyrimidinic endonuclease (APE).

34 , reduces the ability of the substrate to be nicked by Rep78 in vitro.

35 These sequence motifs can be specifically nicked by the viral Rep protein required for the initiat

36 During AAV DNA replication, the TRs are nicked by the virus-encoded Rep proteins at the terminal

37 ering, incorporation of fluorescein into the nicked chromosomal DNA (TUNEL assay), and mono- or oligo

38 g, and incorporation of fluorescein into the nicked chromosomal DNA (TUNEL assay).

39 forces or by replication of single-stranded nicked chromosomes.

40 In reactions with nicked circular DNA (RFII), AN and AN/L3 hydrolyzed exon

41 les the removal of undesired linear DNA when nicked circular DNA has been enzymatically prepared from

42 imer ends; (iii) loading mthPCNA onto singly nicked circular DNA; and (iv) supporting mthPolB-catalyz

43 In the reverse direction (linear ssDNA + nicked circular dsDNA --> circular ssDNA + linear dsDNA)

44 ild-type RecA protein, the resolution to the nicked circular dsDNA product is reduced.

45 double-stranded (ds) DNA --> linear ssDNA + nicked circular dsDNA), the MmsA protein appears to prom

46 ircular plasmid DNA in either supercoiled or nicked circular form often are contaminated with undesir

47 on by peroxyl and hydroxyl radicals into the nicked circular form was also investigated.

48 We have analyzed repair of nicked circular heteroduplex DNA in extracts of Exo1-def

49 O6-methylguanine (MeG), we have constructed nicked circular heteroduplexes that contain a single MeG

50 extracts, as judged by in vitro assay using nicked circular heteroplex DNAs.

51 s from the fluctuations of DNA writhe in the nicked circular molecules which are specified by the val

52 However, efficient packaging is achieved for nicked circular plasmid DNA, but not covalently closed p

53 Supercoiled, relaxed covalently closed, and nicked circular plasmid DNAs were packaged inefficiently

54 eaction, allowing the generation of a simple nicked circular product rather than complex networks of

55 n bias favoring deletion over inversion in a nicked circular substrate containing two symmetrized FRT

56 was required for transcription of linear or nicked circular templates but not of super coiled DNA.

57 Rad17-RFC binds to nicked circular, gapped, and primed DNA and recruits the

58 ites at synapsis, which we investigate using nicked-circular DNA molecules.

59 singly, only linear and supercoiled DNA, not nicked-circular DNA, can completely displace Smc2/4 preb

60 etely displace Smc2/4 prebound to a labeled, nicked-circular DNA.

61 at different sites, and loading PCNA onto a nicked, closed circular substrate with a unique Hx resid

62 investigated the flexibility of two kinds of nicked DNA and AT dinucleotide repeats.

63 odel" in which the ZnF loads ligase III onto nicked DNA and conformational changes deliver DNA into t

64 The FEN-catalysed reaction properties of nicked DNA and flap structures possessing an extrahelica

65 We observed that nicked DNA and nicked-THF flap DNA were poor substrates

66 copy, we show that reverse gyrase recognizes nicked DNA and recruits a protein coat to the site of da

67 PARP-1, a nuclear enzyme, binds tightly to nicked DNA and synthesizes poly(ADP-ribose) as an early

68 PARP-1 activity, since a mixture containing nicked DNA and the PARP-1 ABDEF domains has only basal e

69 d is activated by mononucleosomes containing nicked DNA and which target PARP3 trans-ribosylation act

70 3' mispaired, and beta-l-dioxolane-cytidine nicked DNA are 2.3, 61.2, and 98.8 min(-1), respectively

71 NTase), OB-fold, and latch, that envelop the nicked DNA as a C-shaped protein clamp.

72 sts of three structural modules that envelop nicked DNA as a C-shaped protein clamp: a nucleotidyltra

73 analog beta-l-dioxolane-cytidine terminated nicked DNA as well as for DNA containing a tetrahydrofur

74 These conjugates are proposed to form nicked DNA dumbbell structures in which a stilbenedicarb

75 alyze the ATP-dependent ligation of a singly nicked DNA duplex but not blunt-end joining.

76 sis UvrD has an unwinding preference towards nicked DNA duplexes and stalled replication forks, repre

77 To mimic large numbers of nicked DNA duplexes we used a technique that produces ni

78 ligase catalyzed strand-joining on a singly nicked DNA in the presence of a divalent cation (magnesi

79 nt LigB catalyzed strand joining on a singly-nicked DNA in the presence of a divalent cation and NAD(

80 ligase catalyzed strand joining on a singly nicked DNA in the presence of a divalent cation and NAD(

81 rted by the observation that, in solution, a nicked DNA intermediate accumulates before linearization

82 ity of Arabidopsis cell extracts to ligate a nicked DNA intermediate.

83 p or bottom DNA strand first to generate two nicked DNA intermediates, the enzyme has a preference fo

84 Induced fit of the nicked DNA into a distorted conformation when bound with

85 The repair of phosphodiester bonds in nicked DNA is catalyzed by DNA ligases.

86 We discuss how breakage of nicked DNA may be mechanistically linked to trapping.

87 e cleavage intermediate is mostly top-strand nicked DNA on a single-site plasmid.

88 NA ligase III that each bind specifically to nicked DNA over intact duplex DNA.

89 generated by endonucleases and degrades the nicked DNA polynucleotide.

90 displaces bound glycosylases and retains the nicked DNA product, suggesting that APE1 acts in vivo to

91 pens after catalysis, leading to a cytotoxic nicked DNA repair intermediate.

92 The free sulfhydryl at the 5'-end of the nicked DNA strand in this trapped covalent complex is sh

93 our data reveal that the enzyme can digest a nicked DNA strand processively over at least 60 nt in a

94 Paradoxically, when DNA ligases encounter nicked DNA structures with abnormal DNA termini, DNA lig

95 Poliota cannot initiate synthesis on a nicked DNA substrate, but fills short gaps efficiently.

96 e reactivity of mycobacterial ligases with a nicked DNA substrate, whereby LigA and LigB display vigo

97 NA end to begin reverse transcription on the nicked DNA substrate.

98 e-44 in forming the protein clamp around the nicked DNA substrate; and (v) the importance of adenine-

99 cture-specific nucleases cleaves branched or nicked DNA substrates and are implicated in a wide range

100 Phage T7 DNA ligase seals nicked DNA substrates and is a representative member of

101 '-deoxycytidine (FdC), and cytidine into two nicked DNA substrates and the subsequent ligation.

102 rt deletions at the rNMP sites by generating nicked DNA substrates bearing 2',3'-cyclic phosphates at

103 Moreover, when acting on the nicked DNA substrates containing 2',3'-cyclic phosphates

104 This investigation used synthetic, nicked DNA substrates possessing either a 5'-phosphate o

105 of a 12mer oligonucleotide into a continuous nicked DNA superhelix.

106 entially the coding DNA strand, generating a nicked DNA target.

107 Attempted replication across a damaged or nicked DNA template can result in the formation of a dou

108 The ability of nicked DNA to withstand forces greater than that at the

109 n times more efficient than LIG1 at ligating nicked DNA under optimal conditions, mainly because of t

110 The nicked DNA was first analyzed using alkaline gel electro

111 Nicked DNA was packaged at full genome length and with t

112 h 5-phosphorylated abasic sugar analogues in nicked DNA which reveal an open site with no obvious int

113 The catalytic region encircles nicked DNA with each of the domains contacting the DNA d

114 ingle-nucleotide-gapped DNA, nicked DNA, and nicked DNA with various lengths of flaps all with a 5'-t

115 nd quenches fluorescence, whereas completely nicked DNA yields a large signal.

116 -substituted DNA, mismatched DNA, gapped and nicked DNA, and DNA with abasic sites).

117 mic duplex, 5'-overhanging duplex, pseudo-Y, nicked DNA, and flap structures.

118 tes, including single-nucleotide-gapped DNA, nicked DNA, and nicked DNA with various lengths of flaps

119 ructure of human DNA ligase I complexed with nicked DNA, computer-aided drug design was used to ident

120 of substrates including partial duplex DNA, nicked DNA, forked DNA structures, blunt duplex DNA and

121 Stimulated by binding to nicked DNA, PARP-1 catalyzes poly(ADP-ribosyl)ation of t

122 Stimulated by binding to nicked DNA, PARP-1 catalyzes poly(ADP-ribosyl)ation of t

123 Most experiments are performed upon nicked DNA, permitting the detachment (unpeeling) of str

124 In the absence of nicked DNA, the Sulfolobus solfataricus DNA ligase has a

125 imple competitive inhibitors with respect to nicked DNA, whereas L82 is an uncompetitive inhibitor th

126 k in duplex DNA but was unable to covert the nicked DNA-adenylate to a sealed phosphodiester.

127 re and irreversibly damaging DNA by trapping nicked DNA-topoisomerase intermediates could make potent

128 GMP to a downstream strand, which sealed the nicked DNA.

129 ows that the protein wraps completely around nicked DNA.

130 removed by incubation with pyrophosphate or nicked DNA.

131 e enzyme to the 5'-phosphate of a 3'-dideoxy nicked DNA.

132 n replication forks encounter endonuclease V-nicked DNA.

133 nits covalently linked to the 5' ends of the nicked DNA.

134 g double-stranded DNA after filling a gap or nicked DNA.

135 d complex formation between DNA ligase I and nicked DNA.

136 uch more complete assembly on unconstrained (nicked) DNA tethers.

137 egrades one nucleotide gap, 3'-recessed, and nicked DNAs, but exhibits no detectable activity on blun

138 ove 3'-tyrosyl residues from 3'-recessed and nicked DNAs, suggesting a potential role in processing c

139 more quickly than negatively supercoiled or nicked DNAs.

140 cascade of hybridization events that yields nicked double helices analogous to alternating copolymer

141 at the enzyme bound both unnicked and singly nicked double stranded DNA with equivalent affinity (Kd

142 ified POLQ showed DNA polymerase activity on nicked double-stranded DNA and on a singly primed DNA te

143 L.donovani TOP1L/TOP1S heterodimer bound to nicked double-stranded DNA captured as a vanadate comple

144 The ligase domain catalysed the sealing of nicked double-stranded DNA designed to mimic a DSB, cons

145 The final produced long nicked double-stranded DNA loses the ability to protect

146 repair of 5'-end single-stranded tails from nicked double-stranded DNA substrates.

147 RNA ligases were not able to modify a 3'p in nicked double-stranded DNA.

148 ded inosine-containing DNA substrate and the nicked double-stranded inosine-containing DNA product, r

149 4) +/- 2.4 x 10(-4) nmol/min when ligating a nicked double-stranded RNA substrate.

150 D200N and D18N mutations were compared using nicked dsDNA and single-stranded DNA (ssDNA) degradation

151 ecific, sequence-independent recognition for nicked dsDNA bent 100 degrees with unpaired 3' and 5' fl

152 lternative pathway that uses either ssDNA or nicked dsDNA donors and that is strongly inhibited by RA

153 ecule level using braids of intact dsDNA and nicked dsDNA with bulges.

154 olymerase to generate single strand flaps on nicked dsDNA.

155 e of DNA ligases is their envelopment of the nicked duplex as a C-shaped protein clamp, they accompli

156 nking the 3'-OH of the nick; the rest of the nicked duplex can be replaced by DNA.

157 e PCNA sliding clamp tethers DNA ligase I to nicked duplex DNA circles, the interaction does not enha

158 On a nicked duplex DNA substrate, the results reveal binding

159 A duplexes we used a technique that produces nicked duplex DNA substrates by hybridization of complem

160 n adjacent 3'OH and 5'-terminal phosphate of nicked duplex DNA.

161 or the stable association of DNA ligase I to nicked duplex DNA.

162 yme has intrinsic specificity for binding to nicked duplex DNA.

163 y to the protein clamp formed by LigA around nicked duplex DNA.

164 e silencing and siRNA accumulated, mostly in nicked duplex form.

165 ally bind and cleave a synthetic HJ to yield nicked duplex molecules.

166 d cuts across the junction point, to produce nicked duplex products in which the nicks can be readily

167 ions that lead to the formation of ligatable nicked duplex products.

168 etrical cleavage of HJ substrates to produce nicked duplex products.

169 intermediate; Rnl2 bound to an adenylylated nicked duplex, captured immediately following step 2; an

170 dehyde residue embedded within the resulting nicked duplex.

171 requires resolution of the DNA junction into nicked-duplex species by the action of a junction-resolv

172 It is ultimately resolved into two nicked-duplex species by the action of a junction-resolv

173 nl) is a template-directed ligase that seals nicked duplexes in which the 3'-OH strand is RNA.

174 Frayed 5' ends of nicked duplexes resemble flap junctions, unifying the me

175 y T4 DNA ligase, confirming the formation of nicked duplexes.

176 No terminal UTP nicked-end labeling (TUNEL)-positive cells, as a detecti

177 yrosine residue of the small subunit and the nicked ends of the scissile DNA strand, mimicking the pr

178 e the possibility of repair by rejoining the nicked ends.

179 hat the single major catalytic defect in the nicked enzyme corresponds to a 20-fold increase in K(m)(

180 ond-order rate constant for reduction of the nicked enzyme, as measured in anaerobic stopped-flow exp

181 two-step mechanism in which the DNA is first nicked, followed by hairpin formation.

182 Hence, the nicked form accumulates as a transient intermediate.

183 Using a nicked form of phage SP6 RNA polymerase in this study su

184 e for Mus81-type substrates such as D loops, nicked four-way junctions, and 3' flaps.

185 In addition to linking nicked/fragmented DNA molecules back into a contiguous d

186 gle-strand nicked, linearized (double-strand nicked), fully relaxed, partially relaxed (topoisomers),

187 Unphosphorylated RPA initially binds to nicked heteroduplex DNA to facilitate assembly of the MM

188 te of that observed with otherwise identical nicked heteroduplex DNA.

189 system depends on MutL alpha incision of the nicked heteroduplex strand and dNTP-dependent synthesis-

190 A repair assay utilising a nicked heteroduplex substrate with a GT or a GG mismatch

191 ha incision to the discontinuous strand of a nicked heteroduplex.

192 cut arising from the flexible nature of the nicked HJ intermediate.

193 Here we report that a nicked HJ is the preferred substrate of endogenous and r

194 In addition, although nicked HJs are specific substrates for a number of enzym

195 up to Mus81-Eme1's main activity of cleaving nicked HJs during meiosis in S. pombe.

196 itions observed for the resolution of mobile nicked HJs suggest that these cleavage positions are det

197 rther reveal that recognition of 3'-flap and nicked Holliday junction substrates by Mus81-Mms4 involv

198 ) ( approximately 1 min(-1)) and include the nicked Holliday junction, 3'-flapped and replication for

199 stimulate Mus81 endonuclease activity on the nicked Holliday junctions and 3' flap.

200 uing substrate preference of this enzyme for nicked Holliday junctions opens the possibility that cro

201 EME2 cleaves 3'-flaps, replication forks and nicked Holliday junctions, and exhibits limited endonucl

202 diates such as displacement loops (D-loops), nicked Holliday junctions, or 3' flaps but not intact Ho

203 wnstream duplex, such as fork structures and nicked Holliday junctions.

204 DNA in trans to provoke excision of separate nicked homoduplexes argues against one-stage (concerted)

205 SceI in complex with DNA substrates that are nicked in either the top or bottom strands.

206 n blot technique, the gene was predominantly nicked in the nontranscribed strand compared with the tr

207 The lack of tight binding to a nicked inosine product accounts for the increased rate o

208 enzyme with religation so impaired that the nicked intermediate accumulates during plasmid relaxatio

209 l DNA repair pathways is the ligation of the nicked intermediate to form contiguous double-stranded D

210 rpin is formed at one end of the break via a nicked intermediate.

211 two GGA half-sites, are the same for the two nicked intermediates and are unaffected by substrate len

212 identity of several possible abasic site and nicked intermediates generated by monofunctional and bif

213 nuclease Mus81-Mms4/Eme1 incises a number of nicked joint molecule substrates in vitro.

214 s substrates tailor its incision activity to nicked junction molecules.

215 (plasmid) DNA and can separate single-strand nicked, linearized (double-strand nicked), fully relaxed

216 Incision of a nicked mismatch-containing DNA heteroduplex by this four

217 tem reconstituted in vitro, the rejoining of nicked mismatched DNA depended on the presence of APE1,

218 data identify PARP3 as a molecular sensor of nicked nucleosomes and demonstrate, for the first time,

219 toring the ability of the enzyme to ligate a nicked oligonucleotide in which the 5'-terminal phosphat

220 This assay utilizes a nicked oligonucleotide whose 5'-phosphate terminus at th

221 ximately 10-fold compared with corresponding nicked oligonucleotides that lacked abasic sites.

222 The double-stranded DNA is first nicked on one DNA strand and then further cleaved on the

223 x presents the oriT substrate, which is then nicked only by the cognate MobA.

224 owever, covalently closed circular (CCC) and nicked open circular DNA are not substrates for the enzy

225 he tRNA molecule and to discriminate against nicked or damaged species from further maturation.

226 F2 interacts much more strongly with nicked or gapped DNA ligands than does F1, and we presen

227 ed deoxyribonucleotides at the 3' termini of nicked or gapped DNA molecules.

228 5 replication clamp stimulates T4 RNase H on nicked or gapped substrates, where it can be loaded behi

229 and gapped DNA but not onto double-stranded nicked or single-stranded circular DNAs.

230 D, PcrA retains the ability to bind to a pre-nicked oriD, but engages the 3' end of the nick and tran

231 mediate, tertiary folding and docking of the nicked P9 tetraloop, reorganization of the P3 pseudoknot

232 In planar bilayer membranes, trypsin-nicked PA makes cation-selective voltage-gated channels

233 Trypsin-nicked PA63 was then assembled into heptamers through sp

234 ess and the mechanism for the removal of the nicked passenger strand are not known.

235 Ago2 cleavage may facilitate removal of the nicked passenger strand from RISC after maturation.

236 an exonuclease that cleaves and removes the nicked passenger strand from siRNA duplex in a QDE-2-dep

237 n of RISC by degrading the Argonaute2 (Ago2)-nicked passenger strand of duplex siRNA.

238 om toxin-sensitive cells were incubated with nicked PE, toxin unfolding and reducing activities were

239 requires the unwinding of initiator protein-nicked plasmid DNA by the PcrA helicase.

240 anscription-replication protein packaging of nicked plasmid DNA.

241 ry structure of synaptic complexes formed on nicked plasmid DNAs by atomic-force microscopy, in conju

242 ow show that the presence of a mismatch in a nicked plasmid substrate delays nucleosome loading in hu

243 TPase mutant promoted (+) chiral knotting of nicked plasmids, revealing that ATP hydrolysis and the n

244 lly converting ProT into active sigmaPre2, a nicked Pre2 derivative with a single cleaved Ala-470-Asn

245 In T. brucei, the RNA is nicked prior to uridylate insertion and deletion.

246 1 cleaves 5' flaps at their base to create a nicked product for ligation.

247 rimarily reduces the level of binding to the nicked product, suggesting that R118 plays a significant

248 lting temperatures of unnicked substrate and nicked products (no cycling is required).

249 When the enzyme is in excess, the primary nicked products experience a second nicking event on the

250 the branch junction and generates ligatable nicked products from 5'-flap or replication fork substra

251 nover property is caused by tight binding to nicked products.

252 ded or single-stranded inosine substrates or nicked products.

253 is frequently isolated as a proteolytically nicked protein of two fragments that remain noncovalentl

254 support a model in which Mus81-Mms4 cleaves nicked recombination intermediates such as displacement

255 Following editing, nicked RNA is religated by one of two RNA-editing ligase

256 Thus, we infer that the non-specific and nicked-site cleavage activities observed for the native

257 Chemical modifications of a nicked-site substrate at the positions of the scissile p

258 After cleavage of a nicked-site substrate, the half-site that is not covalen

259 al role for RNaseH2 in protecting or marking nicked sites for further processing.

260 nce Pol beta accessibility to the refractory nicked sites in oligonucleosomes.

261 ase to reseal incorrectly or nonspecifically nicked sites was previously developed in our laboratory.

262 ts at individual sites, preferring intact to nicked sites.

263 duct is recombinogenic in cells, because the nicked strand downstream of the cleavage site can dissoc

264 in 7 min, was highly specific (90:1) for the nicked strand, and was strongly mispair-dependent (at le

265 d a 5' nick, with correction directed to the nicked strand, irrespective of which strand contained th

266 hat are rich in polypyrimidine tracts on the nicked strand.

267 our-protein system is strongly biased to the nicked strand.

268 onitored turnover analyses of the intact and nicked streptococcal GlpOs (at [GlpO] approximately 10 m

269 Although breakage of nicked subchromosomal fragments is field strength indepe

270 imulation occur on both a cleavage-sensitive nicked substrate and a less sensitive gapped substrate.

271 uantitatively filling in the gap to create a nicked substrate but did not remove the 5' overhang.

272 Steady state experiments with a nicked substrate containing juxtaposed dC and 5'-phospho

273 opy-based assay further showed that a 100-bp nicked substrate did not remain stably bound by the term

274 However, with a nicked substrate formed by RT, HIV-1 IN removed the over

275 imiting in cell extract mediated-repair of a nicked substrate.

276 We used nicked substrates and a Holliday junction trapping pepti

277 PEC formation on nicked substrates is much less affected by the orientati

278 NA crossings in a transpososome assembled on nicked substrates under conditions that bypass the super

279 recreate an essentially similar topology on nicked substrates, interwrapping both E-R and L-R twice

280 Using singly gapped or nicked templates containing the T7A1 promoter, we have m

281 ther the strand signal or mismatch site, the nicked templates were linearized close to either site an

282 n, although repair synthesis failed using 5'-nicked templates.

283 ucleosome saturation level as in the case of nicked tethers.

284 site in the R(1) subunit remained functional nicked the bottom strand of the sequence, producing CCTC

285 Elastase directly nicked the Stx2d A subunit to A(1) and A(2), an event th

286 site in the R(2) subunit remained functional nicked the top strand, producing CC--TCAGC/GCTGAGG.

287 n addition, FEN1 cleavage on both nicked and nicked-THF flap DNA resulted in a one-nucleotide gapped

288 We observed that nicked DNA and nicked-THF flap DNA were poor substrates for pol beta-me

289 inked glycosylated, and 45% of PpAMA1-3D7 is nicked, though all four recombinant molecules react with

290 transformants, hairpin coding ends could be nicked to generate 3' overhangs and then processed into

291 Un-nicked torsionally relaxed DNA is a poor substrate for t

292 l increase the effective half-life of the 3'-nicked transposon intermediate and consequently enhance

293 rgets for the capture when the target DNA is nicked two nucleotides apart from the TA.

294 Nicked TyrR is identical to full-length TyrR in its oper

295 dition, the two fragments of proteolytically nicked VacA, p37 and p58, interact when coexpressed with

296 The nicked vector was transformed into XL1 blue supercompete

297 n specificities were approximately 100:1 for nicked versus continuous strands, 80:1 for mismatched ve

298 tantially higher binding affinity for ss and nicked versus ds substrate, the interaction with ss RSS

299 ed that some portion of virion proteins are "nicked" via a combination of endoproteolysis and concert

300 2+, hairpins generated by V(D)J cleavage are nicked whereas synthetic hairpins are not.