ライフサイエンスコーパス: nisin

1 rs a new cell, before it encounters external nisin.

2 he NisB protein in the maturation process of nisin.

3 e respects I30W nisin A is similar to native nisin.

4 f NisI interacts with membranes but not with nisin.

5 spores, became susceptible to inhibition by nisin.

6 Nisin(2) inserts into the membrane and we propose this a

7 the potential of complexation to encapsulate nisin (5g/L concentration) using spray-drying technique

8 whereas with the engineered variants, [Trp30]nisin A and [Lys27,Lys31]nisin A, the proportion of pept

9 N-Terminal rings A and B of nisin A and mutacin 1140 (lipid II-binding domain) share

10 Mutacin 1140 and nisin A are peptide antibiotics that belong to the lanti

11 Nisin A binds lipid II and thus disrupts cell wall synth

12 ntly in the mutacin 1140 complex than in the nisin A complex.

13 In these respects I30W nisin A is similar to native nisin.

14 formation may be attributed to the fact that nisin A uses lipid II to form a distinct pore complex, w

15 While the K12L nisin A variant elicited a higher increase in membrane c

16 ical conductance than I30W nisin A, the H27K nisin A variant elicited weaker effects.

17 By contrast, the I30W nisin A variant induced a time-dependent reduction in li

18 re also engineered to generate K12L and H27K nisin A variants, both of which were similar to I30W nis

19 Circular complexes of mutacin 1140 and nisin A were observed by electron microscopy, providing

20 variants, both of which were similar to I30W nisin A with respect to an overall reduction in phosphol

21 citance and electrical conductance than I30W nisin A, the H27K nisin A variant elicited weaker effect

22 ed variants, [Trp30]nisin A and [Lys27,Lys31]nisin A, the proportion of peptide that escapes full pro

23 obacillin I was generally similar to that of nisin A, with increased activity against Streptococcus d

24 onstrated that the mutacin 1140-lipid II and nisin A-lipid II complexes are very stable and capable o

25 demonstrated increased stability compared to nisin A.

26 lipids were coupled to the C-terminus of the nisin A/B ring system to generate semisynthetic construc

27 ce, we tested mutacin IV (a nonlantibiotic), nisin (a single peptide lantibiotics), and three peptide

28 Nisin, a 34 residue lantibiotic produced by strains of L

29 circumvented with a combined approach using nisin, a FDA-approved safe bacteriocin, to inhibit outgr

30 Nisin, a model lantibiotic, has a dual mode of action: i

31 We also found that nisin, a monopeptide lantibiotic, requires LsrS for its

32 ontext of other recently proposed models for nisin action.

33 Expression of aggregation substance after nisin addition to cultures of E. faecalis and the hetero

34 correlation between cell membrane damage and nisin aggregation was observed in vivo.

35 in complexes, it favored the dissociation of nisin-alginate aggregates to form individual complexes.

36 Spray-drying of nisin-low methoxyl pectin or nisin-alginate electrostatic complexes has led to the mi

37 2)P-Labeled cell wall anchor species bind to nisin, an antibiotic that is known to form a complex wit

38 Herein, we describe a nisin analog encoded on the genome of the thermophilic b

39 ificant decreases in resistance to the CAMPs nisin and gallidermin but not polymyxin B.

40 st, the C-terminal domain specifically binds nisin and modulates the membrane affinity of the N-termi

41 ly halloysite, are suitable nanocarriers for nisin and pediocin adsorption.

42 Higher adsorption of nisin and pediocin was obtained on bentonite.

43 tial carriers for the antimicrobial peptides nisin and pediocin.

44 bacterial lipolytic enzymes, the effects of nisin and PLA2 on the degradation of S. aureus lipids we

45 h two chimeric peptides, suggesting that the nisin and prochlorosin biosynthetic enzymes can produce

46 t of CSP but depended on treatment with both nisin and raffinose, showing that coexpression of comW a

47 cyclase enzymes involved in the synthesis of nisin and subtilin (NisC and SpaC, respectively) have be

48 negative bacteria; and like the lantibiotics nisin and subtilin in its ability to inhibit both bacter

49 two more than the five cross-links found in nisin and the most cross-links found in any lantibiotic

50 st-resistant mutants were cross-resistant to nisin and the pAD1-encoded cytolysin.

51 Starch-OS based emulsions not only retained nisin and thymol activities separately, but also exhibit

52 to evaluate the antimicrobial activities of nisin and thymol formulations in cantaloupe juice.

53 succinate (starch-OS) were used to stabilize nisin and thymol in cantaloupe juice-containing fluid.

54 lsions had much greater capability to retain nisin and thymol over the storage and displayed much gre

55 Native nisin and variants elicit an enhanced release of calcein

56 antibiotics, such as vancomycin, ramoplanin, nisin, and bacitracin.

57 n of Fst sensitized cells to the lantibiotic nisin, and Fst-resistant mutants were cross-resistant to

58 The genes encoding nisin are organized in three contiguous operons: nisABTC

59 By using a nisin biosynthesis pathway and its variants as examples,

60 This feature of nisin biosynthesis was exploited in an investigation of

61 responsible for the cyclization step during nisin biosynthesis.

62 riments, we found that fluorescently labeled nisin bound very inhomogeneously to bacterial membranes

63 LanI protein NisI provides immunity against nisin but not against structurally very similar lantibio

64 ynergistic effects of osmotic activation and nisin bypass the limitations of germination as a spore c

65 nt immunity (NisI) can be expressed when the nisin cluster enters a new cell, before it encounters ex

66 The lipid II-binding N-terminus of nisin, comprising the so-called A/B ring system, was syn

67 ipid II aggregates as a function of time and nisin concentration.

68 se synthesis of an analogue of a fragment of nisin containing its ring C.

69 As with all lantibiotics, nisin contains a number of dehydro-residues and thioethe

70 Nisin contains dehydroalanine and dehydrobutyrine residu

71 vely block the antibacterial activity of the nisin-derived lipopeptides.

72 cases, the serine residue, at position 33 of nisin, does not undergo dehydration to Dha33.

73 ged residues in controlling ion flow through nisin-doped membranes.

74 Here we show that the lantibiotic nisin exercises its antibacterial action by targeting pe

75 The antimicrobial peptide nisin exerts its activity by a unique dual mechanism.

76 N-Terminal nisin fragments N1-12 and N1-20 had little effect on pho

77 is likely an evolutionary adaptation of the nisin gene cluster to enable its successful establishmen

78 their band widths specified by the external nisin gradient and cellular nisin immunity.

79 Nisin has attracted much attention recently due to its n

80 Nisin has been commercially used as a food preservative,

81 An Ile1Trp mutation of the N-terminus of nisin has been modelled and docked onto lipid II models;

82 The lantibiotic nisin has been used as an effective food preservative to

83 as it provides little protection against the nisin hinge region variant.

84 These activities can be separated in a nisin hinge-region variant (N20P M21P) that binds lipid

85 ated for the D-Cys3, D-Cys7, L-Cys8, L-Cys11 nisin homologue, while interlinked rings A and B are obt

86 by the external nisin gradient and cellular nisin immunity.

87 propose a model of antibacterial action for nisin in which the terminal amino group of Ile1 targets

88 is operon was upregulated in the presence of nisin in wild-type cells and was more highly expressed i

89 Two diastereomeric analogues of ring C of nisin incorporating a novel norlanthionine residue have

90 f fosfomycin, d-cycloserine, vancomycin, and nisin, indicating a wide-spectrum hypersensitivity to in

91 Structural changes of nisin induced by complexation with pectin or alginate an

92 pCF10, was cloned in a vector containing the nisin-inducible nisA promoter and its two-component regu

93 Enhanced Pel expression from a nisin-inducible plasmid resulted in increased message le

94 opy plasmid pMSP3535, under the control of a nisin-inducible promoter (P(N)), and transformed into pn

95 1 lysin and holin genes were cloned into the nisin-inducible shuttle vector pMSP3545, nisin induction

96 tose induction and significantly reduced the nisin induction capacity, suggesting a potential pivotal

97 the nisin-inducible shuttle vector pMSP3545, nisin induction of holin and lysin caused partial lysis

98 ch was identical to the nisA start site upon nisin induction.

99 In contrast, neither vancomycin nor nisin inhibited outgrowth for the 012 ribotype.

100 olution, osmotic activation solutes enhanced nisin inhibition of superdormant spores to >3.5 log(10)C

101 Nisin is a complex lanthipeptide that has broad spectrum

102 Nisin is a polymacrocyclic peptide antimicrobial with hi

103 Nisin is a post-translationally modified antimicrobial p

104 Nisin is a posttranslationally modified antimicrobial pe

105 The lantibiotic nisin is an antimicrobial peptide produced by Lactococcu

106 The lantibiotic nisin is an antimicrobial peptide that is widely used as

107 The best-known, nisin, is a commercial food preservative.

108 growth, with activities approaching that of nisin itself.

109 cused on the biosynthesis of the antibiotics nisin, lacticin 481, fosfomycin, and bialaphos.

110 ules for the formation of defined and stable nisin-like pores, however, slow membrane depolarization

111 Lipid II mobility due to a steady growth of nisin-Lipid II aggregates as a function of time and nisi

112 n to Lipid II induces the formation of large nisin-Lipid II aggregates in the membrane of bacteria as

113 ore, we observed that the formation of large nisin-Lipid II aggregates induced vesicle budding in gia

114 hat is dependent on the continuous growth of nisin-Lipid II aggregation and probably involves curvatu

115 wever, structural and mechanistic details on nisin-lipid II membrane complexes are currently lacking.

116 as been put forward as the building block of nisin/lipid II binary membrane pores.

117 fferent lipid II structures and a stable 2:1 nisin:lipid II complex formed.

118 Spray-drying of nisin-low methoxyl pectin or nisin-alginate electrostati

119 ation of the role of the NisB protein in pre-nisin maturation.

120 ctrostatic attraction encourages the initial nisin-membrane association.

121 t reduction in lipid mobility, indicative of nisin-membrane surface interactions, as well as a declin

122 irst step in pore formation, mediated by the nisin N-terminus-lipid II pentapeptide hydrogen bond.

123 effect of SDP on cells differs from that of nisin, nigericin, valinomycin and vancomycin-KCl, but re

124 in the biosynthesis of the food preservative nisin (NisB).

125 s the homogenous form of the fully processed nisin (or nisin variant) molecule.

126 it to expand the phenotypic diversity of the nisin pathway by quickly generating a library of 63 path

127 y by altering the regulatory topology of the nisin pathway for constitutive bacteriocin biosynthesis.

128 ile spray-drying promoted the aggregation of nisin-pectin complexes, it favored the dissociation of n

129 wth inhibition curves revealed that LMWC and nisin possessed inverse antibacterial activity against t

130 en when the gradient is driven by structured nisin-producing bacteria and the patterning cells are co

131 id-encoded nisB gene in a range of different nisin-producing strains.

132 Low molecular weight chitosan (LMWC) and nisin, recognized as cationic antibacterial agents (CAAs

133 crocycles A and B within cysteine mutants of nisin residues 1-12 is investigated here by molecular dy

134 sigma(W) contributes to nisin resistance by regulation of a signal peptide pepti

135 The major contribution of sigma(M) to nisin resistance is expression of ltaSa, encoding a stre

136 the presence of extracellular bacitracin and nisin, respectively, the two response regulators (RRs) b

137 Despite this successful use, nisin's stability at pH 7 is limited.

138 We show that nisin sequesters cell wall precursors found in the outer

139 he dual signaling and antibiotic features of nisin, simple synthetic circuits can direct Lactococcus

140 r alginate (2g/L concentration) can preserve nisin structure and antimicrobial activity.

141 mplexation with pectin or alginate preserved nisin structure as well as its antimicrobial activity du

142 sigX and dltA mutants are more sensitive to nisin than wild-type cells.

143 With native nisin this partially processed form represents about 10%

144 ell envelope structure to decrease access of nisin to its lipid II target.

145 Binding of nisin to Lipid II induces the formation of large nisin-L

146 In contrast to nisin treatment, PLA2 treatment does not stimulate endog

147 genous form of the fully processed nisin (or nisin variant) molecule.

148 we now report the recombinant expression of Nisin variants that incorporate noncanonical amino acids

149 ning ncAA that allowed for the expression of Nisin variants with novel macrocyclic topologies.

150 nd nisFEG requires autoinduction by external nisin via signal transducing by NisRK.

151 Wild-type nisin was also docked onto three different lipid II stru

152 of the deletion derivatives by galactose and nisin was compared phenotypically using beta-galactosida

153 A twofold reduction in the MIC value of nisin was obtained against S. aureus, inoculated in a 1.

154 gate the aggregation process of Lipid II and nisin, we assessed its dynamics by single-molecule micro

155 the bacterium on medium containing the CAMP nisin, we isolated a mutant capable of growing in three

156 ic effects of osmotic activation solutes and nisin were associated with loss of membrane integrity.

157 The structural aspects of nisin which facilitate membrane interaction and permeabi

158 actis produce the broad-spectrum bacteriocin nisin, which belongs to the lantibiotic class of antimic

159 Lactococcus lactis produces the lantibiotic nisin, which is widely used as a food preservative.

160 d different conformations in the presence of nisin, which may also have implications for pore formati

161 x, specifically the N-terminal engagement of nisin with lipid II at the pyrophosphate and C-terminus