ライフサイエンスコーパス: nitrate reductase

1 ation, and thereby regulates the activity of nitrate reductase.

2 confirming that the NapA enzyme is the sole nitrate reductase.

3 ation of phosphoenolpyruvate carboxylase and nitrate reductase.

4 low to support respiration by membrane-bound nitrate reductase.

5 the activities of both acid phosphatase and nitrate reductase.

6 , that is constitutive and not inducible for nitrate reductase.

7 biosynthesis of a molybdopterin cofactor of nitrate reductase.

8 required by various molybdoenzymes, such as nitrate reductase.

9 at the first seven genes encode a functional nitrate reductase.

10 rom Arg but via conversion of nitrite by the nitrate reductase.

11 omponents as exemplified by incorporation of nitrate reductase.

12 produced NO, and this requires expression of nitrate reductase.

13 This enhancement required a functional nitrate reductase.

14 tate had no influence on synthesis of either nitrate reductase.

15 , such as the membrane-bound and periplasmic nitrate reductases.

16 ree of homology to prokaryotic dissimilatory nitrate reductases.

17 cteria readily reduce nitrate to nitrite via nitrate reductases.

18 y double mutations in NOA1 and either of the nitrate reductases.

19 e aerobe, expresses two distinct respiratory nitrate reductases.

20 . Typhimurium strains with defects in either nitrate reductase A (narG mutant) or the regulator induc

21 Escherichia coli nitrate reductase A (NarGHI) is a membrane-bound enzyme

22 The molybdenum atom of Escherichia coli nitrate reductase A (NarGHI) is coordinated by two PPT-d

23 Synthesis of active nitrate reductase A and the cytoplasmic, NADH-dependent

24 s with a second set of mutants revealed that nitrate reductase A couples more effectively with ubiqui

25 es present in Escherichia coli suggests that nitrate reductase A, encoded by the narGHI genes, is the

26 te of the only functional nitrate reductase, nitrate reductase A, is located in the cytoplasm, so nit

27 ocess, we knocked down the gene encoding for nitrate reductase, a key enzyme required for the assimil

28 we show that the native localization of the nitrate reductase, a major respiratory complex under ana

29 the biofilm state included those encoding a nitrate reductase, a nitrite reductase, and a nitric oxi

30 sence of protein FA, to enhance the level of nitrate reductase activation achieved on incubation with

31 lent cations among the CRP/FNR (fumarate and nitrate reductase activator protein) superfamily of regu

32 AMP receptor protein)- and FNR (fumarate and nitrate reductase activator protein)-dependent promoters

33 Increases in nitrate reductase activities after exposure of barley pl

34 oli which produced formate hydrogenlyase and nitrate reductase activities only when grown in medium w

35 efects in metabolism including a loss of all nitrate reductase activities, biofilm maturation, and vi

36 that the PA1006 protein is critical for all nitrate reductase activities, growth as a biofilm in a c

37 ures and hypoxic cultures yielded comparable nitrate reductase activities.

38 We also detected nitrate-inducible nitrate reductase activity (2 to 39 nmol min-1 mg-1) in

39 Nitrate reductase activity (NRA) associated with the mic

40 shown to moderately affect fermentation, but nitrate reductase activity and fnr are dispensable for f

41 berated from the membrane, NarGH retains its nitrate reductase activity and forms films on graphite a

42 produced a strain that had aerobic levels of nitrate reductase activity but failed to show hypoxic up

43 vity increased approximately 100-fold, while nitrate reductase activity decreased 10-fold in whole-ce

44 ing the loss of assimilatory and respiratory nitrate reductase activity in a mobA deletion mutant.

45 We measured reduced viologen-dependent nitrate reductase activity in a series of strains with c

46 Consistent with our hypothesis, nitrate reductase activity in colonized plants was twice

47 This indicates that nitrate reductase activity in M. tuberculosis is due to

48 Mycobacterium tuberculosis induces nitrate reductase activity in response to decreasing oxy

49 The narG mutant showed no nitrate reductase activity in whole culture or in cell-f

50 In this hypoxic state, nitrate reductase activity is strongly induced.

51 All eight lines had detectable nitrate reductase activity ranging from 7% to 150% of wi

52 ized that colonized plants would have higher nitrate reductase activity than uncolonized plants becau

53 phenotype in line with a severe reduction in nitrate reductase activity that may be due to the overac

54 subsequently express methyl viologen-linked nitrate reductase activity under aerobic conditions with

55 previous studies had demonstrated that high nitrate reductase activity under substrate-limiting cond

56 The napF operon-encoded nitrate reductase activity was not sensitive to azide, a

57 rains have lost hydrogen sulfide production, nitrate reductase activity, and gas production from gluc

58 elated with enhanced nitrate content, higher nitrate reductase activity, and sustained ammonium conte

59 re or assimilate nitrate and did not express nitrate reductase activity, confirming that the NapA enz

60 reported appearance of a respiratory type of nitrate reductase activity, the increase in glycine dehy

61 5, neither of which contained any detectable nitrate reductase activity, were selected for complement

62 erepressed and expresses very high levels of nitrate reductase activity.

63 yanide was a noncompetitive inhibitor of the nitrate reductase activity.

64 the Wood-Ljungdahl pathway genes and repress nitrate reductase activity.

65 60 and AMC364 lack methyl viologen-supported nitrate reductase activity.

66 ron catalyst inspired by the active sites of nitrate reductase and (per)chlorate reductase enzymes.

67 which are predicted to encode a periplasmic nitrate reductase and eight encode proteins homologous t

68 e cofactors of many metalloenzymes including nitrate reductase and Mo-nitrogenase.

69 ose oxidase and galactose oxidase deactivate nitrate reductase and must be quenched for biosensor app

70 Both membrane nitrate reductase and nitrite reductase enzyme complexes

71 nitrate or nitrite at 15 degreesC, although nitrate reductase and nitrite reductase were still activ

72 we generated knockouts of the genes encoding nitrate reductase and nitrite reductase, resulting in st

73 n of reduction equivalent, and activities of nitrate reductase and nitrite reductase.

74 lates 3-hydroxy-3-methylglutaryl-Coenzyme A, nitrate reductase and sucrose phosphate synthase in vitr

75 A fluorometric assay using nitrate reductase and the NADPH regenerating system was

76 K, which delivers nitrate to the respiratory nitrate reductase and transfers the product, nitrite, to

77 , together with nasBC (encoding assimilatory nitrate reductase) and nasF (required for nitrite reduct

78 and catalase, and lack of motility, oxidase, nitrate reductase, and gelatinase.

79 strains defective in the two membrane-bound nitrate reductases, and also defective in either ubiquin

80 It is well established that assimilatory nitrate reductase (ANR) activity in soil is inhibited by

81 ry effect of ammonium (NH4+) on assimilatory nitrate reductase (ANR) activity in soil is not due to N

82 ed sites in sulfite oxidase and assimilatory nitrate reductase as deduced from crystallography (sulfi

83 icated that two isolates held genes encoding nitrate reductase as the only dissimilatory N-oxide redu

84 are critical for controlling the activity of nitrate reductase, as the formation of polar assemblies

85 stant Staphylococcus aureus in </=6 h: (i) a nitrate reductase assay and (ii) a resazurin microplate

86 Our results were confirmed with the nitrate reductase assay and genomic sequencing.

87 nitrogen cycle and human health, taxonomy of nitrate reductases, assimilatory and dissimilatory nitra

88 to nitrite was accomplished using NADPH and nitrate reductase, (b) excess NADPH, proteins, and inter

89 The construction and characterization of a nitrate reductase-based amperometric electrode for deter

90 Plasma NO3- was reduced to NO2- by nitrate reductase before determination of NO2- concentra

91 lack nitrate reductase Z and the periplasmic nitrate reductase, but express all combinations of narK

92 tein, NarJ, which is not present in purified nitrate reductase, but is required for biogenesis of the

93 nd conclude that 14-3-3 most likely inhibits nitrate reductase by inducing a conformational change th

94 have been found to regulate the plant enzyme nitrate reductase by reversible phosphoserine binding.

95 , alpha, beta, and gamma, of the respiratory nitrate reductase complex in Escherichia coli.

96 ssion of genes coding for the membrane-bound nitrate reductase complex is responsive to CF sputum nit

97 taining bacteriochlorophyll and assimilative nitrate reductase constituted <1% of the sampled bacteri

98 Two of these nitrate reductase-deficient cell lines, nia 3 and nia 25

99 tant with the napA gene encoding periplasmic nitrate reductase deleted could not respire or assimilat

100 eletion mutations in genes encoding membrane nitrate reductase (Delta narGH) and nitrite reductase (D

101 bunit of R. solanacearum's sole assimilatory nitrate reductase, did not grow on nitrate as a sole nit

102 te sensor-response regulator and in membrane nitrate reductase displayed altered motility and biofilm

103 The P. aerophilum nitrate reductase distinguishes itself from nitrate redu

104 ochrome c reductase fragment of spinach NADH-nitrate reductase (EC 1.6.6.1), consisting of the contig

105 al inactivation of the aerobically expressed nitrate reductase eliminated aerobic nitrate reduction,

106 operon and a periplasmic cytochrome c-linked nitrate reductase encoded by the napFDAGHBC operon.

107 Periplasmic nitrate reductase, encoded by the napFDAGHBC operon, fun

108 Also required are respiratory nitrate reductase, encoded by the narGHJI operon, and mo

109 Two distinct nitrate reductases, encoded by narGHI and nasBC, functio

110 The S. Typhimurium genome contains three nitrate reductases, encoded by the narGHI, narZYV, and n

111 sses two membrane-bound proton-translocating nitrate reductases, encoded by the narGHJI and narZYWV o

112 rifier whose genome contains two periplasmic nitrate reductase-encoding gene clusters.

113 Eight of these were further analyzed for nitrate reductase enzyme activity and nitrate reductase

114 r nitrite and electron transport through the nitrate reductase enzyme in narK mutants reveals that Na

115 , and skin commensal bacteria can synthesize nitrate reductase enzyme.

116 coli synthesizes two biochemically distinct nitrate reductase enzymes, a membrane-bound enzyme encod

117 ertion of the M. tuberculosis narGHJI into a nitrate reductase Escherichia coli mutant allowed anaero

118 ate reduction by two assimilatory eukaryotic nitrate reductase (eukNR) enzymes.

119 nts was counteracted by azide, which induced nitrate reductase expression only if the transcriptional

120 study constitutes the first description of a nitrate reductase from a hyperthermophilic archaeon.

121 assimilation, we generated a knockout in the nitrate reductase gene (NR-KO) of the model pennate diat

122 A cloned Arabidopsis thaliana nitrate reductase gene Nia 2 was introduced into each of

123 A 1.8-kb promoter fragment from the nitrate reductase gene NIA1 was identified that acts as

124 sites in the upstream promoter region of the nitrate reductase gene, NIA1, and physically interact un

125 gions, designated as NP1 and NP2, of the two nitrate reductase genes NR1 and NR2, respectively, are s

126 ranscription of the two Arabidopsis thaliana nitrate reductase genes.

127 uctural genes, except for single cis-QTL for nitrate reductase, Glu dehydrogenase, and shikimate dehy

128 h a 14-3-3-binding phosphopeptide, including nitrate reductase, glyceraldehyde- 3-phosphate dehydroge

129 uminex, and No2-/No3- was measured using the nitrate reductase/Griess assay.

130 hen incubated at 100 degrees C, the purified nitrate reductase had a half-life of 1.5 h.

131 synthase-type enzyme and from nitrite using nitrate reductase has been demonstrated previously.

132 Hypotheses that nitrate reductase has direct or indirect roles in nitrat

133 ons, the napF operon, encoding a periplasmic nitrate reductase, has unique features with respect to i

134 Both membrane-bound and periplasmic nitrate reductases have been found in denitrifying bacte

135 The electrode consisted of nitrate reductase held by dialysis membrane onto a Nafio

136 nces appear to be related to the activity of nitrate reductase in cells and to the possible expressio

137 Yet the role of periplasmic nitrate reductase in denitrification has not been clearl

138 Low expression of membrane-bound nitrate reductase in narK mutants was counteracted by az

139 To analyze the function of the periplasmic nitrate reductase in Pseudomonas sp. strain G-179, the n

140 is is consistent with a role for the aerobic nitrate reductase in redox homeostasis.

141 s of acid phosphatase, cysteine protease and nitrate reductase in sap samples from epidermal and meso

142 propose that they should be designated nap (nitrate reductase in the periplasm) genes.

143 These results suggest that the periplasmic nitrate reductase in this strain plays a primary role in

144 loroplasts and nitrite reductase, but not by nitrate reductase, indicated that N(2)O produced by leav

145 at position 543 (Ser543) promotes binding to nitrate reductase inhibitor protein (NIP).

146 ese findings suggest that the membrane-bound nitrate reductase is critical for P. aeruginosa anaerobi

147 al analysis revealed that the membrane-bound nitrate reductase is essential for P. aeruginosa anaerob

148 Since nitrate reductase is likely responsible for NO productio

149 These results show that one of the nitrate reductases is specific for respiration and denit

150 ra crassa, the expression of the nit-3 gene (nitrate reductase) is dependent upon nitrogen derepressi

151 gulated structural gene nit-3, which encodes nitrate reductase, is dependent upon a synergistic inter

152 uctase1 (NR1) and NR2 (genes that encode two nitrate reductase isoforms) was greatly reduced in the n

153 The activity of the more slowly migrating nitrate reductase isozyme (NR1) was induced by NO3- in g

154 Four isogenic lines with different nitrate reductase isozyme combinations were used to dete

155 r all four genotypes indicating that neither nitrate reductase isozyme has a direct role in nitrate u

156 ld type indicating that the NADH and NAD(P)H nitrate reductase isozymes are responsible for most of t

157 Both nitrate reductase isozymes were induced by nitrate and w

158 L.) has NADH-specific and NAD(P)H-bispecific nitrate reductase isozymes.

159 These results indicate that periplasmic nitrate reductase, like fumarate reductase, can function

160 ctor (MoCo) biosynthesis proteins as well as nitrate reductase maturation factor NarJ and component N

161 ed for nitrate reductase enzyme activity and nitrate reductase mRNA production.

162 ared in wild-type Columbia-0 (Col-0) and the nitrate reductase mutant nia1nia2.

163 Interestingly, the membrane nitrate reductase mutant was avirulent in C. elegans, wh

164 Nitrate reductase mutants were isolated from hNP 588 pro

165 ous oxide, we demonstrate that a periplasmic nitrate reductase, NAD(P)-linked and copper-containing n

166 occus pantotrophus can express a periplasmic nitrate reductase (Nap) during aerobic growth.

167 re we report that DnrF selectively repressed nitrate reductase (nap) genes, preventing further NO for

168 This result suggests that the periplasmic nitrate reductase (Nap) of S. gotlandica strain GD1(T) f

169 scherichia coli K-12, encoding a periplasmic nitrate reductase (Nap), encodes seven proteins.

170 ified into three distinct types--periplasmic nitrate reductase (Nap), respiratory nitrate reductase (

171 t, a strain lacking a functional periplasmic nitrate reductase (napA mutant) exhibited a marked growt

172 xample the signal peptide of the periplasmic nitrate reductase (NapA) is bound by a cytoplasmic chape

173 Periplasmic nitrate reductase (NapABC enzyme) has been characterized

174 Periplasmic nitrate reductase (napFDAGHBC operon product) functions

175 plasmic nitrate reductase (Nap), respiratory nitrate reductase (Nar) and assimilatory nitrate reducta

176 copper, zinc superoxide dismutase (SOD1) and nitrate reductase (NaR) coimmobilized on carbon nanotube

177 for a reagentless nitrate biosensor based on nitrate reductase (NaR) from Escherichia coli could be a

178 NO), FNR also regulates genes, including the nitrate reductase (nar) operon, a major source of endoge

179 nitrate differently than the membrane-bound nitrate reductase (Nar), which is generally prevalent am

180 atalysis by eukaryotic molybdenum-containing nitrate reductase (NaR, EC 1.7.1.1-3) were investigated

181 (tnaA), a tryptophan permease (tnaB), and a nitrate reductase (narG), as well as a natural drop in t

182 rification pathway employs inventory such as nitrate reductase NarGH serving M. denitrificans sp. nov

183 e-containing subunit NarI of the respiratory nitrate reductase NarGHI as proteins that interact with

184 coregulation of NasDEF with the respiratory nitrate reductase NarGHI during nitrate respiration.

185 compared with the highly similar respiratory nitrate reductase NarGHI, which has a relatively much lo

186 uctase family of enzymes, which includes the nitrate reductase NarGHI.

187 The expression of nitrate reductase (NarGHI) was examined using a narG-lip

188 and regulatory regions of the membrane-bound nitrate reductase narGHJI operon (Pnar) are fused to a g

189 er altered nitrate control of NarL-dependent nitrate reductase (narGHJI) and fumarate reductase (frdA

190 (star) mutants that constitutively activate nitrate reductase (narGHJI) gene expression and repress

191 m the structural genes of the membrane-bound nitrate reductase (narGHJI) in Paracoccus pantotrophus t

192 trimethylamine oxide reductase (dmsABC), and nitrate reductase (narGHJI), used during anaerobic respi

193 an anaerobic regulator, FNR, and respiratory nitrate reductase, NarGHJI.

194 duced genes, including those for respiratory nitrate reductase, narGHJI.B. subtilis has two distinct

195 ory nitrate reductase (Nar) and assimilatory nitrate reductase (Nas), they are defined by their cellu

196 Among tobacco transformants carrying a nitrate reductase (Nia) construct under the control of t

197 nse of NR2, but not NR1 or the gene encoding nitrate reductase (NiR), to light signals was impaired i

198 te assimilation, including the gene encoding nitrate reductase (NIT1), are subject to repression by a

199 as reporters Chlamydomonas genes that encode nitrate reductase (NITI) and arylsulfatase (ARS2) transc

200 The active site of the only functional nitrate reductase, nitrate reductase A, is located in th

201 nd nor genes (associated, respectively, with nitrate reductase, nitrite reductase, and nitric oxide r

202 s (e.g. those encoding nitrate transporters, nitrate reductase, nitrite reductase, ferredoxin reducta

203 Studies on the diurnal variations of nitrate reductase (NR) activity during the life cycle of

204 Nitrate reductase (NR) activity increased up to 14-fold

205 experiments generally paralleled changes in nitrate reductase (NR) activity.

206 4-3-3 proteins bind to the hinge 1 region of nitrate reductase (NR) and inhibit its activity.

207 The nature of the association between nitrate reductase (NR) and membranes was examined.

208 state inhibition of the NO-generating enzyme nitrate reductase (NR) attenuates NO production in respo

209 The comparative induction of nitrate reductase (NR) by ambient NO3- and NO2- as a fun

210 The inactivation of phosphorylated nitrate reductase (NR) by the binding of 14-3-3 proteins

211 extremely low in alpha-DiT1 leaves, although nitrate reductase (NR) expression and activity remained

212 (NRs), the nucleotide sequences encoding 19 nitrate reductase (NR) genes from 16 species of fungi, a

213 Nitrate reductase (NR) in leaves is rapidly inactivated

214 Nitrate reductase (NR) is a complex molybdenum cofactor

215 Eukaryotic assimilatory nitrate reductase (NR) is a multi-domain protein that ca

216 reduction of nitrate to nitrite catalyzed by nitrate reductase (NR) is considered to be the rate-limi

217 Nitrate reductase (NR) is rapidly inactivated by phospho

218 Spinach leaf NADH:nitrate reductase (NR) responds to light/dark signals an

219 was capable of phosphorylating and rendering nitrate reductase (NR) sensitive to inhibition by 14-3-3

220 inhibited both glutamine synthetase (GS) and nitrate reductase (NR) was highly purified from cauliflo

221 inated phosphorylation and 14-3-3 binding of nitrate reductase (NR), 6-phosphofructo-2-kinase/fructos

222 , the major mechanism of NO synthesis is via NITRATE REDUCTASE (NR), an enzyme of nitrogen assimilati

223 Banding patterns of nitrate reductase (NR), nitrite reductase (NiR), and glu

224 milation in plants by tuning the activity of nitrate reductase (NR), the first and rate-limiting enzy

225 OH1)-mediated hydrogen peroxide (H2 O2 ) and NITRATE REDUCTASE (NR)-mediated nitric oxide (NO) in gua

226 A nitrate reductase (NR)-null mutant of Arabidopsis was co

227 to regulate the expression of genes encoding nitrate reductase (NR).

228 ation of inorganic nitrogen by knocking down nitrate reductase (NR).

229 Recombinant Arabidopsis thaliana NADH:nitrate reductase (NR; EC 1.6.6.1) was produced in the m

230 des were based on sequences from Arabidopsis nitrate reductase (NR2) and the plasma membrane proton p

231 evolutionary mechanisms and relationships of nitrate reductases (NRs), the nucleotide sequences encod

232 The nitrate reductase of the hyperthermophilic archaeon Pyro

233 nitrate reductase distinguishes itself from nitrate reductases of mesophilic bacteria and archaea by

234 ed to determine the role of NADH and NAD(P)H nitrate reductases on nitrate transport and assimilation

235 uctase, narGHJI.B. subtilis has two distinct nitrate reductases, one for the assimilation of nitrate

236 tate levels of nitrate revealed that the two nitrate reductase operons are differentially expressed i

237 al that controls the expression of these two nitrate reductase operons in a differential and compleme

238 Mutants lacking either nitrate reductase or fumarate reductase also had major c

239 In support of this idea, mutants lacking nitrate reductase or fumarate reductase have extreme col

240 ochrome bd oxidase is more advantageous than nitrate reductase or fumarate reductase.

241 Strains lacking nitrate reductase outcompeted fumarate reductase mutants

242 /proton symporter NasA from the assimilatory nitrate reductase pathway, support that NarK1 functions

243 Binding of 14-3-3 proteins to nitrate reductase phosphorylated on Ser543 (phospho-NR)

244 ilation through inhibition of phosphorylated nitrate reductase (pNR) in darkened leaves.

245 hO(-), 2-AdO(-), Pr(i)()O(-)), dissimilatory nitrate reductase (QR' = 2-AdS(-)), and formate dehydrog

246 ma 70-type promoter and an FNR (fumarate and nitrate reductase regulator protein) binding site, both

247 RP (cAMP receptor protein)/FNR (fumarate and nitrate reductase regulatory protein) family of transcri

248 genes, encoding nitrate/nitrite permease and nitrate reductase, respectively, were isolated from the

249 d to a heme-depleted, catalytically inactive nitrate reductase, restoring its nitrate-reducing enzyme

250 f the napFDAGHBC operon encoding periplasmic nitrate reductase results from synergistic transcription

251 ity to alpha- and beta-subunits of microbial nitrate reductase, selenate reductase, dimethyl sulfide

252 on of nitrate was developed using simplified nitrate reductase (SNaR) that was produced by genetic re

253 ory nitrate reduction, cellular locations of nitrate reductases, structural and redox chemistry are d

254 The MPT containing enzymes nitrate reductase, sulphite oxidase, and SN oxide reduct

255 ns still allow proper nitrogen repression of nitrate reductase synthesis.

256 he C-terminal domain of gp91(phox) on a corn nitrate reductase template suggests close proximity of t

257 Here we purify a nitrate reductase that specifically catalyzes the format

258 Unlike the membrane-bound nitrate reductase, the nitrate reduction activity in str

259 te-regulated genes (including those encoding nitrate reductase, the nitrate transporter NRT1, and glu

260 this strain are Mo-dependent enzymes, as is nitrate reductase; thus, transport of molybdate is impor

261 est that S. Typhimurium uses the periplasmic nitrate reductase to support its growth on the low nitra

262 lla cytochrome bd-II oxidase synergized with nitrate reductases to drive luminal expansion, and both

263 f the narGHJI operon encoding membrane-bound nitrate reductase under any growth condition tested.

264 Nitrate reductase utilizes a Moco but its activity was u

265 In contrast, when the anaerobic nitrate reductase was absent, aerobic nitrate reduction

266 The aerobic nitrate reductase was expressed but not utilized in liqu

267 te biosensor based on recombinant eukaryotic nitrate reductase was implemented in commercial screen-p

268 Notably, the expression of respiratory nitrate reductase was not affected by the carbon sources

269 Under anaerobic conditions the activity of nitrate reductase was reduced by only 50%, showing that

270 synthetic source of NO in tomato cotyledons, nitrate reductase was shown to be under strict control o

271 otype (Az12;Az70) which is deficient in both nitrate reductases, was only 13% of the wild type indica

272 ogenase 3 and several molybdoenzymes such as nitrate reductase were also increased.

273 The activities for fumarate and nitrate reductase were lower in ETRA-153, as were the le

274 ction focuses on the features of periplasmic nitrate reductase where the catalytic subunit of the Nap

275 n and use of four isogenic strains that lack nitrate reductase Z and the periplasmic nitrate reductas