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1 precise genetic requirements for alternative nitrogen fixation.
2 -1 carried the genes for carbon fixation and nitrogen fixation.
3 ia or the nitrogenase enzyme responsible for nitrogen fixation.
4 ent infection, terminal differentiation, and nitrogen fixation.
5 ellular environment and gain the ability for nitrogen fixation.
6 ucing soil erosion, and enhancing carbon and nitrogen fixation.
7 tations in HetR render Anabaena incapable of nitrogen fixation.
8 estion and have been shown to play a role in nitrogen fixation.
9 ots and root nodules of M. truncatula during nitrogen fixation.
10  in the processes of both photosynthesis and nitrogen fixation.
11 ened for defects in nodulation and symbiotic nitrogen fixation.
12 ucturally specialized to enable O2-sensitive nitrogen fixation.
13 utative defects in nodule development and/or nitrogen fixation.
14 ines were confirmed with defective symbiotic nitrogen fixation.
15 ges to identify miRNAs involved in symbiotic nitrogen fixation.
16 s coding for lignocellulosic degradation and nitrogen fixation.
17 nesis, bacterial infection, and the onset of nitrogen fixation.
18 ted symbiosomes are structured for efficient nitrogen fixation.
19 ontributed to the evolution of endosymbiotic nitrogen fixation.
20 e genome, to achieve its function related to nitrogen fixation.
21 cterial viability while permitting effective nitrogen fixation.
22 ces dinitrogen (N2) to ammonia in biological nitrogen fixation.
23 cludes most species capable of endosymbiotic nitrogen fixation.
24 roaerophilic conditions that support optimum nitrogen fixation.
25 gene encoding glutamate dehydrogenase during nitrogen fixation.
26 phosphorylation, carbon dioxide fixation and nitrogen fixation.
27  which encodes a key regulator necessary for nitrogen fixation.
28 xidant defenses that are critical to support nitrogen fixation.
29 ein2b double mutant showing severely reduced nitrogen fixation.
30 of such an organization on the regulation of nitrogen fixation.
31 bacteroids, the active form that carries out nitrogen fixation.
32 cterial differentiation that is required for nitrogen fixation.
33 chea, is the major contributor to biological nitrogen fixation.
34 e legume roots for the purpose of biological nitrogen fixation.
35  the symbiosis, forming nodules incapable of nitrogen fixation.
36 decreased total nodule number and defects in nitrogen fixation.
37 urrent upper temperature limit of biological nitrogen fixation.
38 es, such as photosynthesis, respiration, and nitrogen fixation.
39 ophic growth or for ammonia synthesis during nitrogen fixation.
40 dependent mutant lines that are defective in nitrogen fixation.
41  under conditions that are inappropriate for nitrogen fixation.
42 is of the roles of GlnD in the regulation of nitrogen fixation.
43 oth vertical descent and lateral transfer of nitrogen fixation.
44 ion by NifL under conditions appropriate for nitrogen fixation.
45 within the community not including symbiotic nitrogen fixation.
46  evolution may play a key role in biological nitrogen fixation.
47 tion of dinitrogen to ammonium in biological nitrogen fixation.
48 ct effect resulting from abolished symbiotic nitrogen fixation.
49 n to ammonium (DNRA) or source pathways like nitrogen fixation.
50 ignal motile rhizobia required for symbiotic nitrogen fixation.
51  and nitrogen metabolism under conditions of nitrogen fixation.
52 -2/NCR169 deletion mutant restored symbiotic nitrogen fixation.
53 odulation genes, and improved nodulation and nitrogen fixation.
54 r of nitrogenase, is essential for symbiotic nitrogen fixation.
55 e genes, which leads to nodule formation and nitrogen fixation.
56 nd plants that leads to nodule formation and nitrogen fixation.
57 as Cyanothece sp. ATCC 51142, are capable of nitrogen fixation, a highly oxygen-sensitive process, by
58 trogenase is responsible for most biological nitrogen fixation, a prokaryotic metabolic process that
59  growth and yield diminished, but also (iii) nitrogen fixation--a major ecosystem service of legumino
60 of fixed nitrogen rather than a reduction in nitrogen fixation activity remains to be established.
61 ough there was no corresponding reduction in nitrogen fixation activity.
62 n roots, containing few bacteria and lacking nitrogen fixation activity.
63             Knowing this, we investigated if nitrogen-fixation activity could be detected in microcos
64 none of the plasmids could support symbiotic nitrogen fixation, although they can transport dicarboxy
65 fferences in the patterns of respiration and nitrogen fixation among the Cyanothece spp. strains that
66                                   Biological nitrogen fixation, an essential process of the biogeoche
67 the ocean to meet their requirements through nitrogen fixation, an idea supported by recent modelling
68 ranscription of genes required for symbiotic nitrogen fixation, anaerobic and microaerobic respiratio
69 final class that supports low levels of both nitrogen fixation and bacterial nifH expression.
70 al biofilms--in the challenges of cancer, in nitrogen fixation and chelation, in the production of an
71 sis, induction of root nodules and symbiotic nitrogen fixation and denitrification.
72 nition that the Mesoproterozoic evolution of nitrogen fixation and eukaryotic life may have been prom
73  results in irreversible, large increases in nitrogen fixation and growth rates, even after being mov
74 processes including carbon dioxide fixation, nitrogen fixation and hydrogen metabolism.
75 the ability to perform high rates of aerobic nitrogen fixation and hydrogen production appears to be
76  has been shown to perform efficient aerobic nitrogen fixation and hydrogen production.
77 fic coupling in these clusters necessary for nitrogen fixation and implies an evolutionary connection
78 rspective provides an overview of biological nitrogen fixation and introduces three contributions to
79 lasses: one class that supports little to no nitrogen fixation and minimal bacterial expression of ni
80 sion of nifH; another class that supports no nitrogen fixation and minimal bacterial expression of no
81 urther demonstrated that nodule development, nitrogen fixation and nodule metabolism were negatively
82                      Prokaryotes involved in nitrogen fixation and other nitrogen transformations are
83 ption of genes encoding proteins involved in nitrogen fixation and oxidative stress.
84       The filaments can achieve simultaneous nitrogen fixation and oxygenic photosynthesis by coopera
85 ate microbial-dependent soil processes, like nitrogen fixation and pest attack of root crops.
86 trations of iron metalloproteins involved in nitrogen fixation and photosynthesis, as well as nocturn
87 ells and a substantial decrease in symbiotic nitrogen fixation and plant growth.
88 ing genes responsible for key traits such as nitrogen fixation and seed quality.
89 .2 billion years on the origin of biological nitrogen fixation and suggest that molybdenum was bioava
90                    The presence of genes for nitrogen fixation and the biosynthesis of amino acids an
91 ell as the link of S-metabolism to symbiotic nitrogen fixation and the effect of nodules on whole-pla
92 ic hypotheses for biological (and synthetic) nitrogen fixation and to the rational design of improved
93 ng cyanobacterial ancestor, repeated loss of nitrogen fixation and vertical descent, with little hori
94 oxygenic photosynthesis, often together with nitrogen fixation and, thus, are major primary producers
95 ly in the expression of nifH - essential for nitrogen fixation - and could be visualised using marker
96 the aa(3)-type cytochrome oxidase, symbiotic nitrogen fixation, and anoxic nitrate respiration.
97 , including known factors for nodulation and nitrogen fixation, and candidates with previously unexpl
98 dium nitrogen fixation (vnf) and alternative nitrogen fixation (anf) genes, respectively.
99 on-starvation involve down-regulation of the nitrogen fixation apparatus.
100 -standing puzzle(2) is that trees capable of nitrogen fixation are abundant in nitrogen-rich tropical
101 demonstrate that cyanobacteria abundance and nitrogen fixation are correlated with hypoxia occurring
102 f synthetic chemicals compromising symbiotic nitrogen fixation are increased dependence on synthetic
103 the biological reduction of N(2) to ammonia (nitrogen fixation) as well as the reduction of a number
104 t of this work is that it indicates that the nitrogen fixation-associated ClpX must recruit ClpP, for
105 ukaryotes are an integral part of biological nitrogen fixation at a global scale.
106  photosynthesis during the day and microoxic nitrogen fixation at night.
107 processes: photosynthesis during the day and nitrogen fixation at night.
108 m Trichodesmium showing increased growth and nitrogen fixation at projected future high CO2 levels su
109                                   Biological nitrogen fixation (BNF) is the largest natural source of
110 (ETSP), may support high rates of biological nitrogen fixation (BNF), yet little is known about the N
111 inputs through the enhancement of biological nitrogen fixation (BNF).
112 alustris have multiple enzymatic options for nitrogen fixation, but in contrast to reports on other n
113                                              Nitrogen fixation by cyanobacteria supplies critical bio
114                                              Nitrogen fixation by diazotrophic cyanobacteria is a cri
115 mber of genes considered to be essential for nitrogen fixation by FeFe nitrogenase, including nifM, v
116 spectrometry we directly imaged and measured nitrogen fixation by individual bacteria within eukaryot
117                              Optimization of nitrogen fixation by rhizobia in legumes is a key area o
118                                              Nitrogen fixation by the free-living organism Azotobacte
119 text of modeling intermediates in biological nitrogen fixation by the nitrogenase enzymes and the ind
120                                           If nitrogen fixation by UCYN-A is not stimulated by elevate
121 rain PCC 7120 produces specialized cells for nitrogen fixation called heterocysts.
122 ed light harvesting, oxygen respiration, and nitrogen fixation capabilities, as well as anaerobic fer
123 omplexes might facilitate the development of nitrogen fixation catalysts.
124                                   Biological nitrogen fixation constitutes the main input of fixed ni
125                     Two proteins involved in nitrogen fixation contain ferredoxin-type [4Fe4S] cluste
126                                 No genes for nitrogen fixation could be identified, but genes for a m
127    This increase in the temperature limit of nitrogen fixation could reveal a broader range of condit
128            These mutants do not suppress the nitrogen fixation defect.
129                         Efficient biological nitrogen fixation depends on metabolites produced by and
130 ombined nutrient supply mechanisms including nitrogen fixation, diffusive transport and vertical entr
131 ayed and reduced nodulation but effective in nitrogen fixation (dNod+/- Fix+), and 11 supernodulating
132 st (plant growth per gram nodule growth) and nitrogen fixation efficiency (H(2) production by nitroge
133 c interactions can lead to wide variation in nitrogen fixation efficiency, and it is not uncommon tha
134  a direct result of changes in nodulation or nitrogen fixation efficiency.
135   The soybean-B. elkanii symbiosis has a low nitrogen-fixation efficiency, but B. elkanii strains are
136 tinct from that in Cyanobacteria, suggesting nitrogen fixation evolved separately in the two lineages
137 he subset of plants that carry out symbiotic nitrogen fixation expresses hemoglobins that scavenge an
138                Here we reveal that symbiotic nitrogen fixation facilitates the cultivation of special
139 ur, and complex carbon oxidation, carbon and nitrogen fixation, fermentation, hydrogen metabolism, an
140 e formation of infected nodules defective in nitrogen fixation (Fix(-)).
141                      Given the importance of nitrogen fixation for legume growth, we evaluated the im
142 erimental P and ammonium enrichment promoted nitrogen fixation gene (nifH) expression in Anabaena.
143 tified novel regulatory cascades controlling nitrogen-fixation gene expression in Geobacter sulfurred
144 own in other nitrogen-fixing microorganisms, nitrogen-fixation gene regulation in G. sulfurreducens i
145       This study provides a new paradigm for nitrogen-fixation gene regulation.
146                   Phylogenies constructed on nitrogen fixation genes are not in agreement with the tr
147 regulatory protein controls transcription of nitrogen fixation genes in Azotobacter vinelandii by mod
148 that a cascade of the Sinorhizobium meliloti nitrogen fixation genes is induced as the concentration
149        We demonstrate that rearrangements of nitrogen fixation genes take place normally in the devH
150                     Furthermore, a subset of nitrogen fixation genes was moderately induced during ch
151 lycoside hydrolases attacking celluloses and nitrogen fixation genes were overrepresented in N. corni
152 zobium isolates, lacking both nodulation and nitrogen fixation genes, to have their genomes sequenced
153 t activates transcription of the majority of nitrogen-fixation genes and represses a gene encoding gl
154  was adapted to the microxic environment and nitrogen-fixation genes were expressed.
155 ermination the expression of a subset of the nitrogen-fixation genes whose transcription is activated
156                                   Biological nitrogen fixation has been investigated beginning with t
157 at led to the gain of symbiotic actinorhizal nitrogen fixation have never been investigated.
158 e in the global N cycle, rates of biological nitrogen fixation have proven difficult to quantify.
159 s differentiate into anaerobic factories for nitrogen fixation (heterocysts), requires the transport
160                                              Nitrogen fixation, however, is thought to be a negligibl
161 n A. brasilense prevails under conditions of nitrogen fixation, illustrating a strategy by which cell
162 he isotopic mass balance between nitrate and nitrogen fixation implies a 17 to 27 per cent increase i
163 to improve nodule development and biological nitrogen fixation in acid-stressed soils.
164 upper temperature limit (72.7 degrees C) for nitrogen fixation in alkaline, terrestrial hydrothermal
165 kinase domain are responsible for regulating nitrogen fixation in an oxygen-dependent manner.
166 nfer a streamlined genome expression towards nitrogen fixation in both UCYN-A lineages.
167           Heterocysts, cells specialized for nitrogen fixation in certain filamentous cyanobacteria,
168 the miR172-mediated improvement of symbiotic nitrogen fixation in common bean, the most important gra
169        No significant change was observed in nitrogen fixation in either the High CO2 or Greenhouse t
170 egulate infection, nodule organogenesis, and nitrogen fixation in L. japonicus.
171  is an essential micronutrient for symbiotic nitrogen fixation in legume nodules, where it is require
172                The symbiosis responsible for nitrogen fixation in legume root nodules is initiated by
173                                   Biological nitrogen fixation in legumes occurs in nodules that are
174                                              Nitrogen fixation in legumes requires the development of
175 y been recognized for their contributions to nitrogen fixation in marine environments, a function pre
176 ystems, using the open problem of biological nitrogen fixation in nitrogenase as an example.
177 e for cytokinin in regulating nodulation and nitrogen fixation in response to nitrate as ckx3 phenoty
178 e expression of genes required for symbiotic nitrogen fixation in response to oxygen concentration vi
179  with the conspicuous capacity for symbiotic nitrogen fixation in root nodules, specialized plant org
180 f molecular tools for genetic improvement of nitrogen fixation in soybeans.
181 y constrain hypotheses regarding Fe-mediated nitrogen fixation in synthetic and biological systems.
182 ia in legumes account for a large portion of nitrogen fixation in the biosphere.
183                                              Nitrogen fixation in the legume-rhizobium symbiosis is a
184 e describe the trafficking of molybdenum for nitrogen fixation in the model diazotrophic bacterium Az
185 ium UCYN-A, one of the major contributors to nitrogen fixation in the open ocean, lives in symbiosis
186 able heterocysts but is Fox(-), incapable of nitrogen fixation in the presence of oxygen.
187 ) and might have played an important role in nitrogen fixation in the primitive upper terrestrial atm
188           The majority of genes required for nitrogen fixation in this organism are encoded in the ni
189                   Establishment of symbiotic nitrogen-fixation in legumes is regulated by the plant h
190 sis, root nodules are the sites of bacterial nitrogen fixation, in which atmospheric nitrogen is conv
191 2, dnf3, dnf4, dnf6, and dnf7) defective for nitrogen fixation induce the above genes.
192 en chemical cycle for group 6 metal-mediated nitrogen fixation into a thermally promoted process with
193 ds as a major barrier to engineer biological nitrogen fixation into cereal crops by direct nif gene t
194                                              Nitrogen fixation is an energy-demanding process requiri
195                       Most of the biological nitrogen fixation is catalyzed by the molybdenum nitroge
196                     We conclude that oceanic nitrogen fixation is closely tied to the generation of n
197              The ability of hrrP to suppress nitrogen fixation is conditioned upon the genotypes of b
198                                              Nitrogen fixation is crucial for maintaining biological
199 riability, the global distribution of marine nitrogen fixation is difficult to determine from direct
200                                   In nature, nitrogen fixation is one of the most important life proc
201          We find that the recent increase in nitrogen fixation is the continuation of a much larger,
202                       Fe-mediated biological nitrogen fixation is thought to proceed via either a seq
203 the enzyme system responsible for biological nitrogen fixation, is believed to utilize two unique met
204 spite the essential importance of biological nitrogen fixation, it has been impossible to quantify th
205 zes were rare, but the plant characteristics nitrogen fixation, life cycle (annual or perennial), and
206                  These experiments show that nitrogen fixation may play an important role in naphthal
207 n additional cellular processes that include nitrogen fixation, metabolism, and metal transport.
208  between P-deficiency and factors related to nitrogen fixation/metabolism.
209                  Transcripts associated with nitrogen fixation, methanogenesis and dissimilatory sulf
210 endent records suggests that the increase in nitrogen fixation might be linked to Northern Hemisphere
211 c processes and therefore suggest biological nitrogen fixation, most probably using molybdenum-based
212 F, exoY, bacA, and nifH) in the defective in nitrogen fixation mutants.
213 odularity of a refactored Klebsiella oxytoca nitrogen fixation (nif) gene cluster (16 genes, 103 part
214 fixation in this organism are encoded in the nitrogen fixation (nif) gene clusters, whereas genes spe
215  marine bacterial metagenomes, we noted that nitrogen fixation (nif) genes were conspicuous by their
216  been replaced by an epsilon-proteobacterial nitrogen fixation (NIF) system consisting of two compone
217  atmosphere, requiring a biologic cycle with nitrogen fixation, nitrification and denitrification.
218 ion, photosynthetic nitrogen use efficiency, nitrogen fixation, nitrogen-leaching losses, gross nitro
219 fective in nodule emergence, elongation, and nitrogen fixation (Nod+/- Fix-), one mutant with delayed
220                                    Symbiotic nitrogen fixation occurs in nodules, specialized organs
221 oot nodules where the majority of biological nitrogen fixation occurs.
222 ence a limiting stoichiometry for biological nitrogen fixation of eight electrons/protons, and provid
223 dicates the potential to estimate biological nitrogen fixation of legume symbioses not only in labora
224 he temporal separation of photosynthesis and nitrogen fixation on global gene regulation and cellular
225 n amounts are not significantly regulated by nitrogen fixation or leaf ureide content.
226     Summary Post-translational regulation of nitrogen fixation, or switch-off, in the methanogenic ar
227 ous findings and the potential for symbiotic nitrogen fixation, our results strongly support the hypo
228    Results indicate the genetic capacity for nitrogen fixation over the entire range of temperatures
229 tion implies a 17 to 27 per cent increase in nitrogen fixation over this time period.
230 cluster from Klebsiella oxytoca encoding the nitrogen fixation pathway for converting atmospheric N(2
231        This approach is applied to a 16-gene nitrogen fixation pathway, which is broken into pools co
232 dels the final steps of proposed Fe-mediated nitrogen fixation pathways.
233 e accompanied by irreversible shifts in diel nitrogen fixation patterns, and increased activity of a
234 o higher nitrogen fixation per plant even if nitrogen fixation per nodule mass was similar to that in
235  nodulation and, as a consequence, to higher nitrogen fixation per plant even if nitrogen fixation pe
236        Although the underlying principles of nitrogen fixation predict unicellular nitrogen-fixing cy
237 (3), given that the physiological cost(4) of nitrogen fixation predicts the opposite pattern: fixers
238                          Many aspects of the nitrogen fixation process by photochemistry in the Titan
239 olites are presented and correlated with the nitrogen fixation process.
240 cific role of (Nif)IscA in the maturation of nitrogen fixation proteins are currently unknown.
241                    Our results indicate that nitrogen fixation rates are highest in the Pacific Ocean
242 l effect was positively associated with high nitrogen fixation rates in roots.
243                            Here we determine nitrogen fixation rates in the world's oceans through th
244 n nitrogen content, biomass accumulation and nitrogen fixation rates in wheat roots.
245      The factors that control the growth and nitrogen fixation rates of marine diazotrophs such as Tr
246                                              Nitrogen fixation rates of the globally distributed, bio
247 imilation, increased root exudates to supply nitrogen fixation, reduced dark respiration, and improve
248 aralogue is not duplicated within any of the nitrogen fixation regions of the genome, to achieve its
249                            It is involved in nitrogen fixation-related copper homeostasis and targets
250 ins the largest intact contiguous cluster of nitrogen fixation-related genes.
251  or replace damaged proteins (in the case of nitrogen fixation) remains to be determined.
252 llenging aspect of nodule physiology is that nitrogen fixation requires the cycling of amino acids be
253                    A main goal of biological nitrogen fixation research has been to expand the nitrog
254 ygen sensing, in diverse processes including nitrogen fixation, respiration and photosynthesis.
255 e 51142 performs oxygenic photosynthesis and nitrogen fixation, separating these two incompatible pro
256               Iron is critical for symbiotic nitrogen fixation (SNF) as a key component of multiple f
257                                    Symbiotic nitrogen fixation (SNF) occurs in specialized organs cal
258      Evidence is presented to suggest that a nitrogen fixation-specific paralogue of ClpX is used to
259 functions, one of which is activation of the nitrogen fixation-specific regulatory protein NifA.
260 itrification zones, supporting the view that nitrogen fixation stabilizes the oceanic inventory of fi
261                                              Nitrogen-fixation-subunit-U (NFU)-type proteins have bee
262 nd mechanism, hypothetically the products of nitrogen fixation supplied by heterocysts, must also pla
263 d that cross-talk occurs between the various nitrogen fixation systems, and that expression and fine-
264 t a late evolutionary leap in cyanobacterial nitrogen fixation terminated a long history of nitrogen-
265 y to support higher Crocosphaera biomass and nitrogen fixation than if they did not have this reduced
266 mplete a highly efficient chemical cycle for nitrogen fixation that is mediated by a set of well-char
267                                              Nitrogen fixation - the reduction of dinitrogen (N2) gas
268 karyotes and eukaryotes as follows: the NIF (nitrogen fixation), the ISC (iron-sulfur cluster), and t
269                                   Biological nitrogen fixation, the conversion of atmospheric N2 to N
270                                   Biological nitrogen fixation, the conversion of atmospheric nitroge
271             During the process of biological nitrogen fixation, the enzyme nitrogenase catalyzes the
272 strategies to study the prokaryotic trait of nitrogen fixation, the enzyme-catalyzed reduction of N(2
273 teria and develop a new organ specialized in nitrogen fixation: the nodule.
274 though for some cellular activities, such as nitrogen fixation, there is a full complement of genes i
275                       They conduct symbiotic nitrogen fixation through endosymbiotic relationships wi
276 es a microoxic intracellular environment for nitrogen fixation through the differentiation of semireg
277  enzymes involved in both photosynthesis and nitrogen fixation to determine how Trichodesmium allocat
278 ld-type MtNramp1 in mutant nodules increased nitrogen fixation to normal levels.
279  allowing oxygen-sensitive processes such as nitrogen fixation to occur in cyanobacterial cells.
280 ofactors in biological pathways ranging from nitrogen fixation to respiration.
281 ay account for the difference in response of nitrogen fixation under elevated CO2 to that reported pr
282 tes that some diazotroph groups may increase nitrogen fixation under elevated pCO2 .
283 process, by separating oxygen evolution from nitrogen fixation using a day-night cycle.
284 The subsurface organism has the capacity for nitrogen fixation using a nitrogenase distinct from that
285 The genome encodes genes for photosynthesis, nitrogen fixation, utilization of xenobiotic organic sub
286 ndent diazotophy are encoded by the vanadium nitrogen fixation (vnf) and alternative nitrogen fixatio
287                                   Biological nitrogen fixation was estimated through (15)N isotope di
288 ter stages of nodule development when active nitrogen fixation was occurring.
289 gnitude greater than anammox and DNRA, while nitrogen fixation was undetectable.
290  assimilation and are required for effective nitrogen fixation, we tested the relationship of sugars
291 n mutants exhibited wild-type nodulation and nitrogen fixation when they were inoculated onto Medicag
292  understanding of the factors that influence nitrogen fixation, which may include iron, nitrogen-to-p
293  inherent capacity for symbiotic atmospheric nitrogen fixation, which provides economically sustainab
294 onents is a common feature used to integrate nitrogen fixation with global cellular physiology.
295 for their ability to carry out endosymbiotic nitrogen fixation with rhizobial bacteria, a process tha
296 ether this enzyme was required for symbiotic nitrogen fixation with soybean (Glycine max cv. Williams
297 unexpected role for ethylene in the onset of nitrogen fixation, with the Ljein2a Ljein2b double mutan
298 strain CJ2 revealed several genes conferring nitrogen fixation within a 65.6 kb region of strain CJ2'
299 incompatible processes of photosynthesis and nitrogen fixation within the same cell, unicellular nitr
300  proposed as intermediates of (bio)catalytic nitrogen fixation, yet experimental evidence to support

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