ライフサイエンスコーパス: nitrosation

1 ion has been used as an index for endogenous nitrosation.

2 ly be applied for the detection of protein S-nitrosation.

3 ion of free NO as the primary mechanism of S-nitrosation.

4 onine requirements arising from homocysteine nitrosation.

5 sms, and obtained evidence of critical thiol nitrosation.

6 link dG-to-dG is an important product of DNA nitrosation.

7 and to which extent this effect depends on S-nitrosation.

8 to the N-nitrosoamidinium ion and reversible nitrosation.

9 ited slower reaction pathways required for S-nitrosation.

10 y, and this effect was also potentiated by S-nitrosation.

11 ttranslational modification of proteins by S-nitrosation.

12 DNA-binding by Cmr was severely impaired by nitrosation.

13 reas electron-withdrawing ones decelerated N-nitrosation.

14 ing NO, an effect that is not prevented by S-nitrosation.

15 bly via reaction(s) of intracellular trans-S-nitrosation.

16 ometry were used to quantify the degree of S-nitrosation.

17 free thiols and site-specific analysis of S-nitrosation.

18 of which have been reported as targets of S-nitrosation.

19 combined effects of amine reactivity toward nitrosation and amine volatility.

20 like GSNO, NO does not elicit homocysteine S nitrosation and compensatory increases in methionine bio

21 Nitrosation and denitrosation of caspase 3 was correlate

22 their activity, it has been suggested that S-nitrosation and denitrosation of cellular thiols are fun

23 sulted in a marked decrease in intracellular nitrosation and diminished NO-induced posttranslational

24 We here show that N-nitrosation and heme-nitrosylation are indeed as ubiquit

25 at neutral pH could be attributed to abiotic nitrosation and if N2O was consumed during N2 formation.

26 or and acute exercise reduced iNOS-induced S-nitrosation and increased insulin sensitivity in the mus

27 ported that compounds in garlic may suppress nitrosation and inhibit carcinogenesis.

28 The highly disparate rates of aromatic nitrosation and nitration, despite the very similar (ele

29 ectron-transfer) mechanism for both aromatic nitrosation and nitration.

30 rculating pool of NO metabolites (oxidation, nitrosation and nitrosylation products) were measured in

31 Nitrosation and oxidation in the regulation of gene expr

32 lational modifications of p21ras including S-nitrosation and S-glutathiolation have been demonstrated

33 Cysteine S-nitrosation and S-sulfination are naturally occurring po

34 proteomic analysis demonstrates that both S-nitrosation and S-sulfination are widespread, yet exhibi

35 tissues reveals distinct profiles for both S-nitrosation and S-sulfination.

36 Since thiols can undergo nitrosation and the cell membrane is rich in thiol-conta

37 s posttranslational protein modifications, S-nitrosation, and genome-wide epigenetic modifications th

38 to diverse modifications, such as oxidation, nitrosation, and lipidation.

39 books as electrophilic aromatic bromination, nitrosation, and nitration, respectively.

40 d liver modifications involved both S- and N-nitrosation, and RBC S-nitrosothiol formation emerged as

41 transfer mechanism in protein nitration and nitrosation are discussed.

42 Dietary N-nitroso compounds and endogenous nitrosation are important carcinogenic factors, but huma

43 eous when intracellular reactions of trans-S-nitrosation are to be studied.

44 ciated from cSrc because of increased Cav1 S-nitrosation at Cys(156), leading to cSrc activation, con

45 hat this particular action of Trx requires S-nitrosation at Cys-69.

46 e plasmatic NO reaction products and limit S-nitrosation at low NO flux.

47 the selectivity and redox dependence of Trx nitrosation at physiologically relevant concentrations a

48 ghly diffusible stable gas NO could initiate nitrosation at sites of neutrophil infiltration.

49 t, MitoSNO, to determine how mitochondrial S-nitrosation at the reperfusion phase of myocardial infar

50 ditions could be a favourable medium for Tau nitrosation, attention should be paid to potential situa

51 r activity that increases with the degree of nitrosation, but S-nitroso derivatives show the greatest

52 , these effects are further potentiated by S-nitrosation, but this posttranslational modification is

53 Our results indicate that, in addition to nitrosation by the .NO derivative dinitrogen trioxide (N

54 tion (by measuring carbonyl groups), protein nitrosation (by measuring nitrosamines), and proteolysis

55 ather than NO autoxidation, explaining why S-nitrosation can compete effectively with nitrosylation.

56 The iNOS self-nitrosation characterized here appears appropriate to he

57 The acidic nitrosation chemistry of nine acyclic secondary and tert

58 are presented of a theoretical study of the nitrosation chemistry of pyrroline 1 (X = CH(2)), imidaz

59 as a source of nitrosonium for nitrosothiol nitrosation, completing the catalytic cycle.

60 ential tests revealed that even under strong nitrosation conditions, polyDADEPC and related lower-ord

61 Sirt1 S-nitrosation correlated with Zn(2+) release from the cons

62 lational modification of Sirt1 by cysteine S-nitrosation correlates with increased acetylation of Sir

63 uene) had various impacts on the extent of N-nitrosation, depending on the iron level.

64 The efficiency of PFC-mediated S-nitrosation depends on the amount of PFC in aqueous solu

65 ion of this residue, precluding hemoglobin S-nitrosation, did not change total red blood cell S-nitro

66 t not to NO2(-), combined with the lack of S-nitrosation during anoxia alone or by NO2(-) during norm

67 isothermal titration calorimetry as well as nitrosation experiments using S-nitrosocysteine demonstr

68 ates in nitration (Figure 8) but only one in nitrosation (Figure 7).

69 To further address the role of S-nitrosation for the overall antiapoptotic effect to Trx,

70 the reported oxygen-dependent promotion of S-nitrosation from SNO-Hb involves biochemical mechanisms

71 tion reactions; however, the kinetics of Trx nitrosation has not previously been investigated.

72 seudo-first-order rate constants for amidine nitrosation in aqueous acetic acid with excess nitrite a

73 e involvement of nitric oxide (NO) -mediated nitrosation in cell signaling and pathology.

74 Constitutive protein S-nitrosation in cells was detected by analysis with bioti

75 sulfur compounds found in garlic may inhibit nitrosation in humans.

76 N-Nitrosation in oxygenated nitric oxide (NO middle dot) s

77 bitors, decreased interferon-gamma-induced S-nitrosation in procaspase-9.

78 otentially be applied for the detection of S-nitrosation in protein systems.

79 However, the analysis of protein S-nitrosation in situ has been difficult because of the ab

80 S-nitrosation in the brain shows regional differences and

81 ults indicate that ONOO- may contribute to S-nitrosation in vivo and that direct nitrosation of thiol

82 -nitrosylation are indeed as ubiquitous as S-nitrosation in vivo and that the products of these react

83 y, aged iNOS-null mice were protected from S-nitrosation-induced insulin resistance.

84 binding assays were consistent with Sirt1 S-nitrosation inhibiting binding of both the NAD(+) and ac

85 S-Nitrosation is a post-translational modification of prot

86 S-nitrosation is a posttranslational, oxidative addition o

87 Nitrosation is an important reaction elicited by nitric

88 also raise the intriguing possibility that N-nitrosation is directly involved in the modulation of pr

89 A paradox of S-nitrosation is that only a small set of reactive cystein

90 Biomarkers of nitrosation, lipoperoxidation, and cytotoxicity were mea

91 itionally, pharmacologic agents disrupting S-nitrosation markedly increased cisplatin toxicity, where

92 dings suggest that the functional effects of nitrosation may be organized to occur within discrete do

93 of GSNO, indicative of a direct nucleophilic nitrosation mechanism with elimination of HOO-.

94 s mutagenic, in the present article tyramine nitrosation mechanisms have been characterized in order

95 igma-complex, whereas this Wheland adduct in nitrosation merely represents a high energy (transition-

96 toward elucidating the mechanism by which S-nitrosation modulates a protein's function is identifica

97 dation peaks and only glyphosate is prone to nitrosation, n-nitroso glyphosate and glufosinate were u

98 e nitrosylation (e.g., DAF radical + NO) and nitrosation (NO+ addition).

99 xidase (MPO), a mediator of inflammation, on nitrosation observed during NO autoxidation.

100 To better understand how nitrosation occurs in biological systems, we assessed th

101 Little is known about whether such nitrosation occurs in physiological conditions and, if s

102 during normoxia places constraints on how S-nitrosation occurs in vivo and on its mechanisms of card

103 Although trans-nitrosation occurs with methanol, 1 degree, 2 degree, an

104 el of NO in the media was also observed, and nitrosation of 2,3-diaminonapthalene was increased great

105 QTOs were prepared by nitrosation of 2,4-quinolinediols.

106 Nitrosation of 2-amino-3-methylimidazo[4,5-f]quinoline (

107 benzoxazinone-3-sulfonamide was prepared by nitrosation of a beta-ketosulfonamide followed by intram

108 Here we show that S-nitrosation of adult human hemoglobin (Hb A(0)) or sickl

109 Nitrosation of amine, thiol, and hydroxyl residues can m

110 rite with no significant risk of artifactual nitrosation of amines.

111 our-membered ring lactones, resulting in the nitrosation of amino carboxylic acids, the fraction of E

112 This study examines nitrosation of aminothiones in acidic solutions and re-e

113 c-oxide synthase (NOS) inhibitors regulate S-nitrosation of an initiator caspase, procaspase-9, in a

114 In contrast, S-nitrosation of ATP synthase alpha subunit in DHF hearts

115 steric mechanisms for hemoglobin, based on S-nitrosation of beta-chain cysteine 93, raise the possibi

116 a-chain (betaCys93) and, specifically, for S-nitrosation of betaCys93 to form S-nitrosohemoglobin (SN

117 hypothesis is that red meat facilitates the nitrosation of bile acid conjugates and amino acids, whi

118 Nitrosation of biological thiols is believed to be media

119 nitrosyl group, and in one subunit, partial nitrosation of bound NOHA.

120 Nitrosation of bovine serum albumin with acidified NaNO2

121 , our data support an important role for the nitrosation of brain proteins.

122 131, R135, K150, and H154) that facilitate S-nitrosation of C151.

123 act as an anti-apoptotic factor via trans-S-nitrosation of caspase 3.

124 isplatin treatment, resulting in augmented S-nitrosation of caspase-3 and prolyl-hydroxylase-2, the e

125 Neither S-nitrosation of caspase-3 nor induction of Hsp70 appeared

126 demonstrate that modulation of NO-mediated S-nitrosation of cellular proteins is strongly associated

127 The nitrosation of cellular thiols has attracted much intere

128 Reversible S-nitrosation of complex I slows the reactivation of mitoc

129 of complex I is afforded by the selective S-nitrosation of Cys39 on the ND3 subunit, which becomes s

130 S-Nitrosation of cysteine beta93 in hemoglobin (S-nitrosoh

131 competes for DAF-2, whereas AA decreases the nitrosation of DAF-2 to a larger extent, possibly becaus

132 Quenching of DHR oxidation by azide and nitrosation of diaminonaphthalene were exclusively obser

133 modifluoromethyl)trimethylsilane followed by nitrosation of difluorinated organozinc species with an

134 olites mediate the oxidation, nitration, and nitrosation of DNA bases, amino acids, and lipids.

135 Our results show that S-nitrosation of endogenous procaspase-9 occurs in the HT-

136 nes with a biotin tag enabled us to reveal S-nitrosation of endogenous procaspase-9 that was immunopr

137 osation reactions, a systematic study of the nitrosation of ethylbenzene, phenethylamine, and tyramin

138 of nitrite (20 nM/min) but failed to elicit nitrosation of extracellular 2,3-diaminonapthalene.

139 processes and are inversely linked to the S-nitrosation of GAPDH and (ii) that the NO sensitivity of

140 rmed that the NO effects are all linked to S-nitrosation of GAPDH at Cys-152.

141 Nitrosation of GSH could theoretically proceed via inter

142 nstrate that GSNO binding does not precede S-nitrosation of GSTP1-1.

143 It involves nitrosation of H2O2 at pH> or = 12.5 by isoamyl or butyl

144 S-nitrosation of Hb (SNO-Hb) may confer vasodilatory prope

145 ity and R-state character that result from S-nitrosation of Hb S would be expected to decrease its po

146 NO breathing increases S-nitrosation of hemoglobin beta-chain cysteine 93, howeve

147 tch method were used to confirm endogenous S-nitrosation of iNOS.

148 (S)-nitrosation of Keap1 may contribute to nuclear accumulat

149 Aging increased iNOS expression and S-nitrosation of major proteins involved in insulin signal

150 Nitrosation of MDEA, a tertiary amine, is not catalyzed

151 We found that protection is due to the S-nitrosation of mitochondrial complex I, which is the ent

152 oxide by cardiac myoglobin and subsequent S-nitrosation of mitochondrial membrane proteins reduced m

153 f mitochondrial respiration as a result of S-nitrosation of NADH: ubiquinone oxidoreductase (complex

154 When comparing the rate of DEANO-mediated nitrosation of Ng with other sulfhydryl-containing compo

155 -1-(3-pyridyl)-1-butanone (NNK) is formed by nitrosation of nicotine and has been identified as the m

156 -1-(3-pyridyl)-1-butanone (NNK) is formed by nitrosation of nicotine and has been identified as the m

157 omponent in cigarette smoke and is formed by nitrosation of nicotine.

158 propose a cationic chain mechanism in which nitrosation of nitrosothiol produces a nitrosated cation

159 elective, and convenient method for the ipso-nitrosation of organotrifluoroborates using nitrosonium

160 Late bloomer: Nitrosation of peracetylated sialic acid glycosides foll

161 conjunction with ischemia led to extensive S-nitrosation of protein thiols across all cellular compar

162 osure to ischemia alone results in minimal S-nitrosation of protein thiols.

163 rly neurodegeneration identified increased S-nitrosation of proteins important for synapse function,

164 ucible nitric oxide synthase (iNOS) in the S-nitrosation of proteins involved in the early steps of t

165 ccumulating evidence has demonstrated that S-nitrosation of proteins plays a critical role in several

166 to quantify the extent of NO2(-)-dependent S-nitrosation of proteins thiols in vivo Using this approa

167 rosylation reaction that is coupled to the S-nitrosation of sGC cysteines.

168 NOS1-dependent S-nitrosation of SOCS1 impairs its binding to p65 and targ

169 is bimolecular attack by N(2)O(3); (iii) the nitrosation of taurine affords ethanesultone (ES), which

170 An S-nitrosation of the aconitase [4Fe-4S] center catalyzed b

171 sation pathway is general and results in the nitrosation of the amine Ph2NH and alcohol (t)BuOH to gi

172 benzothiazolines (2) were synthesized by the nitrosation of the corresponding 2-iminobenzothiazolines

173 ations of FL(5) versus FL(5)-NO reveal how N-nitrosation of the fluorophore ligand brings about the f

174 The nitrosothiol 2 was prepared via direct S-nitrosation of the hydrochloride of L-cysteine ethyl est

175 d medium to nitrous acid, which leads to the nitrosation of the indole that is generated by tryptopha

176 ctivation, we show that NO activates TACE by nitrosation of the inhibitory motif of the TACE prodomai

177 n aged mice is mainly mediated through the S-nitrosation of the insulin-signaling pathway.

178 The data show that S-nitrosation of the zinc tetrathiolate cysteine results i

179 in the presence of Zn(2+), consistent with S-nitrosation of the Zn(2+)-tetrathiolate as the primary s

180 ge DNA by deamination of DNA bases following nitrosation of their primary amine functionalities.

181 Although the nitric oxide (.NO)-mediated nitrosation of thiol-containing molecules is increasingl

182 ; 2b), which is extensively used for trans-S-nitrosation of thiol-containing proteins, has a limited

183 The results suggest that oxidation and nitrosation of thiols by superoxide and NO are determine

184 ute to S-nitrosation in vivo and that direct nitrosation of thiols or other nucleophilic substrates b

185 ced apoptosis to a degree similar to hTrx, S-nitrosation of this protein, which lacks Cys-69, failed

186 ogy studies have shown that the product of C-nitrosation of tyramine is mutagenic, in the present art

187 The nitrosations of MEA, DEA, and MMEA are first order in ni

188 The chemical changes (oxidation/nitrosation) of meat proteins during digestion lead to a

189 Protein S-nitrosation on cysteine residues has emerged as an impor

190 t the hypothesis that the reaction occurs by nitrosation on the imino nitrogen, followed by the addit

191 lobin), and Tyr-130 (beta-globin) as well as nitrosation on Tyr-24 (alpha-globin) were identified.

192 a significant factor in the advent of either nitrosation or oxidation chemistry in biological systems

193 and their functions can be modified by thiol nitrosation or oxidation.

194 veral of these phytochemicals either inhibit nitrosation or the formation of DNA adducts or stimulate

195 tivities of these enzymes are inhibited by S-nitrosation, our data thus indicate that modulation of i

196 This nitrosation pathway is general and results in the nitros

197 This is consistent with nitrosation potentiation by MPO, not peroxynitrite.

198 The uric acid nitration/nitrosation product may play a pivotal role in human pat

199 2), this result suggesting that the yield of nitrosation products in the human stomach would increase

200 The principal nitrosation products were amides derived from the amino

201 types of DNA were lower than the morpholine nitrosation rate constant by a factor of approximately 1

202 ) yields close to unity, validating the slow nitrosation rates hypothesized for tertiary amines.

203 The antioxidant or inhibiting nitrosation reaction properties of vegetables and fruits

204 The role of nitrite in the nitrosation reaction was probed at elevated temperatures

205 ts into identifying the protein targets of S-nitrosation reactions and their potential role in cell f

206 Nitrosation reactions are quenched by O-2, while the oxi

207 analysis and spectroscopic studies show that nitrosation reactions are sustained at sulfur, oxygen, n

208 Nitrosation reactions span a diverse range of applicatio

209 Nitrosation reactions sustained at additional nucleophil

210 To determine the kinetic course of nitrosation reactions, a systematic study of the nitrosa

211 cell-surface PDI reacts with NO, catalyzes S-nitrosation reactions, and facilitates NO transfer from

212 tilized for the NO(+) transfer processes and nitrosation reactions.

213 r thioredoxin (Trx) in controlling protein S-nitrosation reactions.

214 rts have studied thermodynamic end points of nitrosation reactions; however, the kinetics of Trx nitr

215 one via reductive alkylation with acetone, N-nitrosation, reduction, and cyclization.

216 We, therefore, suggest that S-nitrosation regulates activation of endogenous procaspas

217 Protein S-nitrosation represents a recently described form of post

218 Reversal of S-nitrosation resulted in full restoration of Sirt1 activi

219 ns suggested that Zn(2+) loss due to Sirt1 S-nitrosation results in repositioning of the tetrathiolat

220 Although GSNO inactivated hBCATm, neither S-nitrosation, S-glutathionylation, nor dimerization could

221 ide (NO) modification, which can result in S-nitrosation, S-thiolation, or disulfide bond formation.

222 using quantitative proteomics to determine S-nitrosation site occupancy.

223 s occurs, and the functional importance of S-nitrosation sites across the mammalian proteome, remain

224 Here we define two specific non-heme iNOS nitrosation sites discovered by combining UV-visible spe

225 that PAF stimulates hyperpermeability via S-nitrosation (SNO) of adherens junction proteins.

226 function in a cGMP-independent manner, via S-nitrosation (SNO), a redox-based modification of cystein

227 Protein S-nitrosation (SNO-protein), the nitric oxide-mediated pos

228 nd inhibits complex I by posttranslational S-nitrosation; this dampens electron transfer and effectiv

229 te in [Cu(II)](kappa(2)-O2N) that leads to S-nitrosation to give the S-nitrosothiol RSNO and copper(I

230 sformation ("metabolic shunting") from thiol nitrosation to heme nitrosylation.

231 The sensitivities of N-nitrosation to these intra- and intermolecular electroni

232 he only dipeptide that underwent significant nitrosation under these conditions.

233 Renin-angiotensin system-induced Cav1 S-nitrosation was associated with increased Cav1-endotheli

234 The rate of nitrosation was found to vary in a systematic way with t

235 Sirt1 S-nitrosation was reversed upon exposure to the thiol-base

236 ever, the mechanism of Sirt1 inhibition by S-nitrosation was unknown.

237 M: disulfide bond, S-glutathionylation and S-nitrosation were assessed.

238 These sites of nitrosation were confirmed at the peptide level using a

239 mechanisms and kinetic laws of tryptophan N-nitrosation were determined.

240 trations of IgG modified by chlorination and nitrosation were measured in synovial fluids from inflam

241 In a similar fashion, the nitrosations were accelerated in polar solvents but were

242 ased, such that there was a 95% reduction in nitrosation when the fluxes of NO and O-2 were nearly eq

243 cysteine residues that can be subjected to S-nitrosation, whereby this process often acts as an activ

244 the most favoured reaction in tyramine is C-nitrosation, which generates mutagenic products.

245 d in hyperoxia-exposed GGT(enu1) mice except nitrosation, which showed a diminished decrease compared

246 rimental results suggest a mechanism for the nitrosation, whose rate-limiting step is bimolecular att

247 ituted methylamines, followed by their alpha-nitrosation with sodium nitrite and subsequent base medi

248 their R-state conformations in response to S-nitrosation, with increased oxygen affinity and decrease

249 An increase in nitrosation within eggs is evident seconds after insemin

250 er processes; however, consumption of NO and nitrosation within intact cells were exponential.

251 Maximum nitrosation yields were obtained at an oxygen concentrat