ライフサイエンスコーパス: nitrosylate

1 ysteine antibody indicated that PARP-1 was S-nitrosylated.

2 e zinc-tetrathiolate cysteines of eNOS are S-nitrosylated.

3 the majority of mitochondrial caspase-9 is S-nitrosylated.

4 lated to produce NO, the exogenous tTG was S-nitrosylated.

5 of exogenous reagents (three of 21) or as S-nitrosylated.

6 mplex formation was impeded when actin was S-nitrosylated.

7 re, we show that PSD-95 is physiologically S-nitrosylated.

8 tension (pO(2) approximately 10 mmHg), NO S-nitrosylates 1 of approximately 50 free cysteines per ry

9 pO2, nanomolar NO activates the channel by S-nitrosylating a single cysteine residue.

10 nson's or Alzheimer's disease, that PDI is S-nitrosylated, a reaction transferring a nitric oxide (NO

11 Prior work has shown that hyperoxia causes S-nitrosylated actin (SNO-actin) formation, which mediates

12 ectrospray-ionization mass spectrometry, and nitrosylated actin was glutathiolated by reduced GSH.

13 nted polymerization normally observed with S-nitrosylated actin, VASP binding to actin, elevated Rac

14 ) regulates cellular function, in part, by S-nitrosylating active site thiol groups of proteins.

15 heterologously expressed human DDAH II was S-nitrosylated after cytokine induced expression of the in

16 e treated whole-heart homogenates with the S-nitrosylating agent S-nitrosoglutathione and determined

17 Interestingly, the NO donor and nitrosylating agent SNAP reversed the depressed RyR2 ope

18 imicked by S-nitroso-glutathione (GSNO; an S-nitrosylating agent).

19 tive care or care augmented by 20 ppm of the nitrosylating agent, ethyl nitrite, blended into the ven

20 scle cells by S-nitrosocysteine (CSNO), an S-nitrosylating agent, in human umbilical vein endothelial

21 We suggest that a nitrosylating agent, possibly derived from HNO, is produ

22 Irrespective of the type of S-nitrosylating agent, S-nitrosylated proteins formed upon

23 cule S-nitrosoglutathione (GSNO) and other S-nitrosylating agents can cause full maturation of the ab

24 cum) Bright Yellow-2 cells, an increase in S-nitrosylating agents occurred.

25 ata suggest that GSNO is one of a class of S-nitrosylating agents that act independently of the class

26 ort that 1) GSNO is only one of a class of S-nitrosylating agents that, at low micromolar concentrati

27 ney 293 cells treated with S-oxidizing and S-nitrosylating agents, and verify that the assay is highl

28 gher oxides, such as N(2)O(3), were actually nitrosylating agents.

29 panied by increased nitrotyrosine formation, nitrosylated alpha-synuclein, and microglial activation.

30 -permeable S-nitrosothiols and that sGC is S-nitrosylated and desensitized in the tolerant, treated t

31 asis, which were reflected by increases in S-nitrosylated and nitrated proteins in the lungs from inf

32 Thus, DR4 residue C336 becomes S nitrosylated and promotes apoptosis following NO-Cbl tre

33 f the HR, the bacterial effector HopAI1 is S-nitrosylated and that this modification inhibits its pho

34 Consequently, nitrosylated and thereby inactivated caspase 3 or cathep

35 Ca(2+), up to 15 cysteines were found to be nitrosylated and this modification resulted in an inhibi

36 fore, we investigated whether Panx1 can be S-nitrosylated and whether this modification can affect ch

37 findings demonstrate that iNOS-derived NO S-nitrosylates and activates cPLA(2)alpha in human cells.

38 domain, binds COX-2 with the generated NO S-nitrosylating and activating the enzyme.

39 is progressively PKA-hyperphosphorylated, S-nitrosylated, and depleted of the phosphodiesterase PDE4

40 Here we show that cPLA(2)alpha protein is S-nitrosylated, and its activity is enhanced by nitric oxi

41 RyR2s are oxidized, nitrosylated, and PKA hyperphosphorylated, resulting in

42 These findings suggest that S-nitrosylated arginase1 can compete with NOS for L-argini

43 in GPCR trafficking, interacts with and is S-nitrosylated at a single cysteine by endothelial NO synt

44 amin, which interacts with NO synthase, is S-nitrosylated at a single cysteine residue (C607) after s

45 r by iNOS induction caused GAPDH to become S-nitrosylated at Cys152.

46 As a result, the HIF-1alpha protein is S-nitrosylated at Cys533 (through "biotin switch" assay) i

47 ) release NO when hemoglobin that has been S-nitrosylated at Cys93 of the beta-chain (betaCys93) tran

48 g cells, a subset of caspase-3 zymogens is S-nitrosylated at the active site cysteine, inhibiting enz

49 ing H2O2 levels in plants, was found to be S-nitrosylated at the onset of both PCDs.

50 Low micromolar levels of S-nitrosylated bovine serum albumin (BSA), but not control

51 The generated NO, in turn, nitrosylates BVR, leading to nuclear translocation where

52 Also, p50 was S-nitrosylated by DETANONOate resulting in inhibition of N

53 ies also indicate that Ras Cys(118) can be S-nitrosylated by direct reaction of Cys(118) with a gluta

54 d from our studies suggest that Ras can be S-nitrosylated by direct reaction of Cys(118) with nitroge

55 We report that NSF is physiologically S-nitrosylated by endogenous, neuronally derived nitric ox

56 A family of proteins S-nitrosylated by iNOS-S100A8/A9 were revealed by proteomi

57 tein when they are one-electron oxidized, or nitrosylated by nitric oxide.

58 e and sarcosine dehydrogenase (SarDH), are S-nitrosylated by NO under strictly anaerobic conditions.

59 In both cells and tissues, GRK2 is S-nitrosylated by SNOs as well as by NO synthases, and GRK

60 Here we show that C. difficile toxins are S-nitrosylated by the infected host and that S-nitrosylati

61 Tissue TG can be poly-S-nitrosylated by the NO carrier, S-nitrosocysteine (CysNO

62 s the binding affinity of calstabin-1 to the nitrosylated channel, inhibited sarcoplasmic reticulum C

63 lt of the conformational change induced by S-nitrosylated CLIC4 that leads to unfolding of the protei

64 thermodynamically controls reactivity toward nitrosylated compounds.

65 PDZ-containing nNOS (nNOSalpha) increases S-nitrosylated CREB with consequent augmented binding on c

66 chment protein receptor (SNARE) complexes by nitrosylating critical cysteine residues of NSF.

67 Previous studies suggested that S-nitrosylated Cys may be flanked by an acid-base motif or

68 c approaches that are capable of identifying nitrosylated Cys residues have been developed.

69 ng proteomic strategy, we identified 1,276 S-nitrosylated cysteine residues [S-nitrosothiol (SNO)] on

70 to ultraviolet light which photo-reverses S-nitrosylated cysteine residues and by co-incubations wit

71 s exposed to UV light, which photoreverses S-nitrosylated cysteine residues and by co-incubations wit

72 Structural analyses revealed that S-nitrosylated cysteine residues were equally distributed

73 Seventy percent of the S-nitrosylated cysteine residues were surrounded by negati

74 the gasotransmitter nitric oxide directly S-nitrosylates cysteine residues in diverse intracellular

75 Two potential S-nitrosylated cysteines in the alpha- and beta-subunits o

76 ield a mixture of penta- and hexa-coordinate nitrosylated cyt c.

77 In the nitrosylated cyt c.CL complex, NO chemically reacted wit

78 cell lymphoma/leukemia (Bcl)-2 or Bcl-X(L), nitrosylated cytochrome c is found in the mitochondria.

79 Nitrosylated cytochrome c is found predominantly in the

80 ylhaemoglobin in the 'tense' T (or partially nitrosylated, deoxy) structure to S-nitrosohaemoglobin i

81 but unlike RyR1, RyR2 was not activated or S-nitrosylated directly by NO.

82 and subjected to the biotin switch assay; S-nitrosylated DR4 was detected in all three cell lines.

83 ion of a mutant form of HDAC2 that cannot be nitrosylated dramatically inhibits Brm expression.

84 effect by NO was reversible since, after the nitrosylated enzyme was reduced with DTT followed by inc

85 Other lipid mediators such as nitrosylated-fatty acids and omega-3 fatty acid-derived

86 2L/TRAIL death receptor DR4 (TRAIL R1) was S nitrosylated following NO-Cbl treatment.

87 The less active, nitrosylated form of NAB1 is found in cells acclimated t

88 SP-D, including significant amounts of an S-nitrosylated form.

89 Specifically, we show that DJ-1 is S-nitrosylated (forming SNO-DJ-1); subsequently, the NO gr

90 Here we report that Prx2 is S-nitrosylated (forming SNO-Prx2) by reaction with nitric

91 ic oxide, its ferredoxin [2Fe-2S] cluster is nitrosylated, forming the dinitrosyl iron complex with a

92 he BST using differentially S-oxidized and S-nitrosylated forms of protein tyrosine phosphatase 1B, a

93 Levels of nitrosylated GAPDH and nitrosylated HDAC2 were increased

94 Nitrosylated GAPDH complexes with the ubiquitin-E3-ligas

95 y cholate, and blocking nuclear transport of nitrosylated GAPDH reduced cholate-induced nitrosylation

96 GSH fully converted nitrosylated GAPDH to the reduced, active enzyme, withou

97 BDNF activates neuronal NOS with the nitrosylated GAPDH/seven in absentia (Siah) homolog comp

98 activate nitric oxide (NO) synthases with NO nitrosylating GAPDH, conferring on it the ability to bin

99 A 2-fold increase in S-nitrosylated GCK was also observed in mouse islets.

100 alyzing TrxR1-dependent denitrosylation of S-nitrosylated glutathione or of HEK293 cell-derived S-nit

101 l responses of Escherichia coli treated with nitrosylated glutathione or the nitric oxide (NO)-genera

102 adequate for the separation of glutathione, nitrosylated glutathione, and glutathione disulfide solu

103 tes generation of nitric oxide (NO), which S-nitrosylates glyceraldehyde-3-phosphate dehydrogenase (G

104 s variations in the contributions from fully nitrosylated Hb and oxidized Hb (MetHb).

105 erive from the functional behaviour of fully nitrosylated Hb, whereas Hb is only partially nitrosylat

106 Levels of nitrosylated GAPDH and nitrosylated HDAC2 were increased in cholestatic human a

107 eme iron was enhanced, and the EPR signal of nitrosylated heme iron became discernible.

108 om dinitrosyl complexes of non-heme iron and nitrosylated heme iron in both K/VP.5-iNOS cells and K/V

109 , S-nitrosothiols, N-nitros-amines, and iron-nitrosylated heme proteins within 1-30 minutes of reperf

110 reasing the yield of S-nitrosohemoglobin and nitrosylated hemes.

111 hway for the formation of beta-93 cysteine S-nitrosylated hemoglobin [SNOHb:S-nitrosohemoglobin], whi

112 ted with rapid formation of erythrocyte iron-nitrosylated hemoglobin and, to a lesser extent, S-nitro

113 The levels of nitrosylated hemoglobin are too low to affect overall he

114 udy reveal a previously unexplored role of S-nitrosylated hemoglobin in cardioprotection.

115 NO inhalation markedly raised total nitrosylated hemoglobin levels, with a significant arter

116 baseline, the arterial and venous levels of nitrosylated hemoglobin were not significantly different

117 ion led to a dose-dependent increase in mean nitrosylated hemoglobin, and at the highest dosage, a si

118 vities and in assays of endogenous Fe- and S-nitrosylated hemoglobin.

119 reaction is immediately autocaptured to form nitrosylated hemoglobin.

120 obin's vacant hemes in a cooperative manner, nitrosylates hemoglobin thiols, or reacts with liberated

121 We further show that S-nitrosylated HIF-1alpha binds to the vascular endothelia

122 evolutionarily conserved and specifically S-nitrosylated in both human and fly NADPH oxidase, sugges

123 that protein disulfide isomerase (PDI) is S-nitrosylated in brains of patients with sporadic neurode

124 ently reported that eNOS is constitutively S-nitrosylated in endothelial cells and that agonists prom

125 involved in contraction and relaxation are S-nitrosylated in laboring and nonlaboring muscle and that

126 ggest that during apoptosis, cytochrome c is nitrosylated in mitochondria and then rapidly released i

127 d that many of these proteins are uniquely S-nitrosylated in only one state of the tissue.

128 We now show that sGC can be S-nitrosylated in primary aortic smooth muscle cells by S-

129 inducible nitric oxide synthase (iNOS) and S-nitrosylated in proinflammatory peritoneal macrophages.

130 We report that eNOS is tonically S-nitrosylated in resting bovine aortic endothelial cells

131 tTG nitrosylated in the absence of Ca(2+) was 6-fold more su

132 recently shown that p65 is constitutively S-nitrosylated in the lung and that LPS-induced injury eli

133 namic cycle exists in which haemoglobin is S-nitrosylated in the lung when red blood cells are oxygen

134 We show that parkin is S-nitrosylated in vitro, as well as in vivo in a mouse mod

135 itrosylated Hb, whereas Hb is only partially nitrosylated in vivo.

136 ntains approximately 84 free thiols and is S-nitrosylated in vivo.

137 urthermore, we identified that gephyrin is S-nitrosylated in vivo.

138 This indicates that NO readily nitrosylates intracellular free heme and prevents its de

139 ative of mechanisms that actively dispose of nitrosylated iron-sulfur centers.

140 r DNICs account for only a minor fraction of nitrosylated iron.

141 that iNOS specifically binds to COX-2 and S-nitrosylates it, enhancing COX-2 catalytic activity.

142 that nitric oxide (NO) reacts with XIAP by S-nitrosylating its RING domain (forming SNO-XIAP), thereb

143 addition, treatment with VEGF-A decreased S-nitrosylated laminin in cultured podocytes.

144 fraction of the spectral signature of fully nitrosylated (largely hexacoordinate) Hb disappears as t

145 We show that SR is physiologically S-nitrosylated leading to marked inhibition of enzyme acti

146 n of Cys-819 allow Cys-110 to be oxidized or nitrosylated, leading to complete inactivation of IDE.

147 The metal-free N-nitrosylated ligand (DAC-NO + H)+ has two conformations:

148 = 1,4,8,11-tetraazacyclotetradecane), the N-nitrosylated ligand DAC-NO, and the Roussin's red salt e

149 systematically investigate oxidized and/or S-nitrosylated mitochondrial proteins and to use a biotin-

150 markedly increased numbers of oxidized and S-nitrosylated mitochondrial proteins following hepatic I/

151 The oxidized and/or S-nitrosylated mitochondrial proteins from I/R-injured mou

152 biotin-N-maleimide-labeled oxidized and/or S-nitrosylated mitochondrial proteins from pair-fed contro

153 sing synthesis of nitric oxide (NO), which S-nitrosylated N-ethylmaleimide sensitive factor (NSF), a

154 eNOS) production of nitric oxide (NO), which nitrosylates N-ethylmaleimide sensitive factor (NSF) and

155 Recently, a new class of nitrosylated NSAID (known as NO-NSAIDs) has been develop

156 O increases S-nitrosylated NSF levels, but S-nitrosylated NSF levels decrease within 3 h after exposu

157 Endogenously synthesized NO increases S-nitrosylated NSF levels, but S-nitrosylated NSF levels d

158 Knockdown of TRX1 increases the level of S-nitrosylated NSF, prolongs the inhibition of exocytosis,

159 d NSF, and endogenous TRX1 removes NO from S-nitrosylated NSF.

160 dition, the class I member HDAC2 was found S-nitrosylated on cysteine, a post-transduction modificati

161 Here we demonstrate that cytochrome c is nitrosylated on its heme iron during apoptosis.

162 Furthermore, we show that BVR is S-nitrosylated on one of three cysteines and that this pos

163 Caspase-3 zymogens were found to be S-nitrosylated on their catalytic-site cysteine in unstimu

164 Finally S-nitrosylated ornithine decarboxylase was isolated from i

165 Further, an increase in S-nitrosylated p50 was detected in cells, and the level wa

166 gene transcription, and nuclear levels of S-nitrosylated p65 correlate with decreased DNA binding of

167 einyl affinity resin to selectively enrich S-nitrosylated peptides reduced by ascorbate followed by n

168 A subset of proteins are constitutively S-nitrosylated, playing roles in the regulation of tissue

169 These thiols may be nitrosylated preferentially during increasing hypoxia or

170 that inhibition of the denitrosylation of S-nitrosylated procaspase-3 mediated by the redox protein

171 on/NO scavenging, we show that the amount of nitrosylated products formed greatly exceeds what can be

172 e, eliminated the light-evoked increase in S-nitrosylated protein immunofluorescence (SNI) in the ret

173 channel alpha1 subunit as the predominant S-nitrosylated protein in membrane fractions, and followin

174 .05 vs. VEH) and increased nitrite and total nitrosylated protein levels.

175 P activity colocalized with immunoreactive S-nitrosylated protein.

176 Here, we discuss how aberrantly S-nitrosylated proteins (SNO-proteins) play a crucial role

177 aced on global, unbiased quantification of S-nitrosylated proteins because of physiologic and oxidati

178 We identified several candidate S-nitrosylated proteins by proteomic analysis following th

179 S-nitrosoglutathione or denitrosylation of S-nitrosylated proteins by reduced GSH.

180 tive of the type of S-nitrosylating agent, S-nitrosylated proteins formed upon exposure to both of th

181 Only a few intracellular S-nitrosylated proteins have been identified, and it is un

182 sing a proteomic approach we characterized S-nitrosylated proteins in citrus leaves exposed to chemic

183 We identify endogenously S-nitrosylated proteins in subcellular organelles, includi

184 Functional analysis of differentially S-nitrosylated proteins indicated their involvement in apo

185 Determination of S-nitrosylated proteins is of great importance for fundame

186 AC requires fewer steps, detects high-mass S-nitrosylated proteins more efficiently, and facilitates

187 The overall patterns of oxidized and/or S-nitrosylated proteins resolved by 2-dimensional polyacry

188 In addition, S-nitrosylated proteins were quantitated using the fluorom

189 e challenge, characterized by the accrual of nitrosylated proteins without a major alteration in cell

190 roteomic identification of over a thousand S-nitrosylated proteins, few S-nitrosylases have been iden

191 modified biotin switch method to identify S-nitrosylated proteins.

192 rols intracellular levels of both GSNO and S-nitrosylated proteins.

193 Accordingly, by decreasing S-nitrosylated Prx2 (SNO-Prx2), overexpression of Srxn1 pr

194 S-Nitrosylated Ras (Ras-SNO) can be formed when NO serves

195 fector of Ras), or GNE rates relative to non-nitrosylated Ras.

196 athways affected by iNOS indicated that NO S-nitrosylated Ras.

197 ithiothreitol, which triggers reduction of S-nitrosylated residues.

198 ted by protein kinase A (PKA), oxidized, and nitrosylated, resulting in depletion of the stabilizing

199 neurons that stargazin is physiologically S-nitrosylated, resulting in increased surface expression.

200 ivation increases iNOS generation of NO to S-nitrosylate RING1A, a key member of the polycomb repress

201 We report two crystal structures of nitrosylated RuBisCO from the red algae Galdieria sulphu

202 led intracellular Ca2+ leak via oxidized and nitrosylated RyR2 channels, activated ER stress response

203 ble guanylyl cyclase to form cGMP, NO also S-nitrosylates selected protein targets.

204 RyR1 macromolecular complex was oxidized, S-nitrosylated, Ser-2844 phosphorylated, and depleted of t

205 After photo-excitation of nitrosylated sGC, only NO geminate rebinding occurs in 7

206 protein (VASP) exhibits high affinity for S-nitrosylated short filamentous actin, which increases ac

207 rt that in neurons and brain tissue NO can S-nitrosylate SHP-2 at its active site cysteine, forming S

208 SHP-2 at its active site cysteine, forming S-nitrosylated SHP-2 (SNO-SHP-2).

209 the NO donor compound S-nitrosoglutathione S-nitrosylates significantly more proteins in mitochondria

210 tates identification and quantification of S-nitrosylated sites by mass spectrometry.

211 e ease with which it can detect individual S-nitrosylated (SNO) proteins in biological samples.

212 Purified, nitrosylated SoxR has transcriptional activity similar t

213 In contrast, nitrosylated SoxR is short-lived in intact cells, indica

214 By native PAGE, formation of S-nitrosylated SP-D in vivo resulted in disruption of SP-D

215 recruitment of effector cells modulated by S-nitrosylated SP-D.

216 the decreased intensities of EPR signals of nitrosylated species.

217 from properties of the target protein, the S-nitrosylating species, or both.

218 rmation feeds back to presynaptic cells to S-nitrosylate SR and decrease D-serine availability to pos

219 inescence in side-by-side assays of multiple nitrosylated standards of varied reactivities and in ass

220 x that can exist in an oxidized, reduced, or nitrosylated state.

221 ith the Cys(85) thiol group in reduced and S-nitrosylated states.

222 The cellular content of S-nitrosylated Syntaxin 4 peaked acutely, within 5 min of

223 treatments, including well known and novel S-nitrosylated targets.

224 whereas specific, high affinity binding of S-nitrosylated TG2 (SNO-TG2) to endothelial surfaces resto

225 We demonstrate that nitric oxide (NO) can S-nitrosylate the ER stress sensors IRE1alpha and PERK.

226 , consistent with the known ability of NO to nitrosylate the Fe(II) center in Fur.

227 NO S-nitrosylates the cysteine residue in the active site of

228 Nitric oxide nitrosylates the WhiB1 iron-sulfur cluster and promotes

229 edback inhibits iNOS gene transcription by S-nitrosylating the trans-activator PARP-1 and decreasing

230 itric oxide (NO) and NO-related species that nitrosylate thiols.

231 set of mitochondrial proteins that can be S-nitrosylated through multiple reaction channels, includi

232 n suggested that multiple cysteines may be S-nitrosylated to regulate Panx1 channel function.

233 This was associated with the appearance of S-nitrosylated TonEBP/NFAT5, as monitored by the biotin-sw

234 Furthermore, S-nitrosylated tTG inhibited platelet aggregation induced

235 The addition of Ca(2+) to nitrosylated tTG was able to trigger the release of NO g

236 f myeloperoxidase as well as chlorinated and nitrosylated tyrosine epitopes in their lesion areas com

237 Nitrosylated tyrosines, a stable marker for NO generatio

238 Up to 20% of myoglobin can be nitrosylated under gastro-intestinal conditions in this

239 revealed more than 300 proteins that were S-nitrosylated upon illumination, many of which are known

240 While wild-type eNOS is robustly S-nitrosylated, we found that S-nitrosylation of the Myr-

241 To identify which cysteine residue(s) was S-nitrosylated, we made single cysteine-to-alanine substit

242 e site Cys(453) determined by isolation of S-nitrosylated wild type but not active site Cys(453) -->

243 bsence of Ca(2+), up to eight cysteines were nitrosylated without modifying TGase activity.