ライフサイエンスコーパス: nonglycosylated

1 ying an MSG gene lacking the UCS expressed a nonglycosylated 130-kDa protein.

2 ycosylated 91-kDa subunit, gp91(phox), and a nonglycosylated 22-kDa subunit, p22(phox).

3 ain protein detected on Western blots as the nonglycosylated 27-kDa form, with overexpression of this

4 e mutant forms of FR-beta were identified as nonglycosylated (29.3 kDa) or glycosylated polypeptides

5 CD20 is a nonglycosylated 33 to 37 kD phosphoprotein involved in B

6 AgSF2 represents a nonglycosylated 40-kDa protein that has novel internal a

7 The nonglycosylated 44-kDa P2X(4) receptor was resistant to

8 In this study, we investigated a unique nonglycosylated active fragment of hepatocyte growth fac

9 that the enhanced epimerization relative to nonglycosylated amino acids is due to a combination of f

10 MR structural characterizations of refolded, nonglycosylated amino-terminal fragments, and another pa

11 ) GalNAc(alpha-->)Thr10 contulakin-G and its nonglycosylated analog were prepared using an Fmoc (9-fl

12 n carcinomas as a combination of both 27-kDa nonglycosylated and 28-kDa glycosylated mature two-chain

13 to determine the solution structures of both nonglycosylated and fucosylated FVII EGF-1 in the presen

14 of the properties of binding of IL-7 to both nonglycosylated and glycosylated forms of the IL-7Ralpha

15 halpy and entropy changes of IL-7 binding to nonglycosylated and glycosylated IL-7Ralpha.

16 44, whereas intracellular LOXL2 (~75-kDa) is nonglycosylated and N-terminally processed, and is prima

17 ecombinant glycosylated full-length Ags, and nonglycosylated and truncated Ags recognized by 4E10 usi

18 ut homology to mammalian sequences labeled a nonglycosylated approximately 41-kDa protein (spx1) on W

19 e capacity of recombinant, histidine-tagged, nonglycosylated BclA (rBclA) given with adjuvant to prot

20 Two nonglycosylated BiP substrates, NS-1kappa light chain an

21 To investigate the disposal of nonglycosylated BiP substrates, we used a nonsecreted ka

22 coproteins > neoglycoproteins based on BSA > nonglycosylated BSA >> free sugars.

23 ate that enhanced binding of 3-6-fold over a nonglycosylated CBM is achieved by the addition of a sin

24 increasing it up to 140-fold relative to the nonglycosylated CBM.

25 Because nonglycosylated channels appear to pass through the secr

26 using purified collagen type I, type IV, and nonglycosylated, commercially available recombinant SLRP

27 ble entropy changes and smaller unfavorable (nonglycosylated complex) and favorable (glycosylated com

28 N- or C-terminal activities relative to the nonglycosylated control peptides.

29 ented N-linked glycosylation, resulting in a nonglycosylated core protein devoid of ATP and ADP hydro

30 of COX-2 protein, which is distinct from the nonglycosylated COX-2 protein generated in the presence

31 e in the E2P form in complex with bufalin (a nonglycosylated CTS with a six-membered lactone) and dig

32 ions (a) a newly synthesized, highly mobile, nonglycosylated, cytosolic receptor that is depleted by

33 Highly methylated, nonglycosylated derivatives of the flavonoid myricetin f

34 re stimulated with peptides representing the nonglycosylated domain of G protein.

35 1A domain (GA733 type 2 motif), and a unique nonglycosylated domain without cysteines (GA733 type 3 m

36 It is a nonglycosylated envelope protein that is regulated as a

37 1 (HSV-1) UL20 gene encodes a 222-amino-acid nonglycosylated envelope protein which forms a complex w

38 hybridomas specific for the glycosylated and nonglycosylated epitope CII(259-273) were developed.

39 osylated EPO had a 20-fold slower k(on) than nonglycosylated EPO, indicating that the core carbohydra

40 ed for ubiquitination or degradation of this nonglycosylated ERAD substrate.

41 the present report, folding comparisons of a nonglycosylated erythropoietin (EPO) mutant are made wit

42 Nonglycosylated erythropoietin bearing acetamidomethyl p

43 HSV-1 infectivity.IMPORTANCE HSV-1 UL20 is a nonglycosylated essential envelope protein that is highl

44 show that glycosylated ETSHR-gp, but not the nonglycosylated ETSHR protein, can react with autoantibo

45 Earlier, we identified two nonglycosylated exofacial regions of erythrocyte band 3

46 Two nonglycosylated exofacial regions of human band 3 in the

47 in bacterial biofilm formation, whereas the nonglycosylated Fap1 peptide mediates bacterial initial

48 IL-8 release from A549 cells stimulated with nonglycosylated flagellar preparations (having less then

49 When the inhibitor and its nonglycosylated form (SmCI N23A mutant) were overproduce

50 newly synthesized Aaop1 is converted to the nonglycosylated form and deposited in the rhabdomeres.

51 elevated concentrations of AVP (100 nM), the nonglycosylated form desensitized to the same extent as

52 wever, the delta Cj1496c mutant expressing a nonglycosylated form of Cj1496c exhibited levels of inva

53 ivity was observed in strains expressing the nonglycosylated form of EmaA.

54 The ability of TgI4.4 to recognize a nonglycosylated form of gI suggests that HSV-1 recogniti

55 deficiency virus (HIV)-1 gp120 and env2-3, a nonglycosylated form of gp120.

56 PDGFRalpha did exhibit binding activity to a nonglycosylated form of PDGFRalpha.

57 ingently regulated than is processing of the nonglycosylated form of the same epitope.

58 uired more extensive processing than did the nonglycosylated form of the same epitope.

59 es: a slow migrating glycosylated form and a nonglycosylated form.

60 The proteins are found in glycosylated and nonglycosylated forms in infected cells.

61 Both glycosylated and nonglycosylated forms of Drm exhibit identical distribut

62 ons bearing combinations of glycosylated and nonglycosylated forms of prM and E could infect mammalia

63 in the steady-state levels of the mono- and nonglycosylated forms of PrP in the brain.

64 V2) from potato (Solanum tuberosum) than its nonglycosylated forms.

65 Here we report that nonglycosylated full-length and C-terminal truncated amy

66 The in vitro fucosylation of the nonglycosylated FVII EGF-1 was achieved by using O-fucos

67 Nonglycosylated G protein also interacted with CNX, but

68 If, however, unfolded nonglycosylated G protein was the starting material, ref

69 A structure of nonglycosylated G(C) reveals an extended conformation th

70 cific hydrophobic amino acid residues on the nonglycosylated GFCC'C" face of CEACAM1 N-terminal domai

71 ein, with high affinity, but it did not bind nonglycosylated gp120 or BSA.

72 It was found to be a nonglycosylated heterodimeric protein, belonging to the

73 eta glycosylation inhibition revealed 30-47% nonglycosylated hFSHbeta associated with four of six hFS

74 Nonglycosylated HIV envelope protein was not associated

75 We also show that nonglycosylated HIV-1(SF-2) gp120 or sodium metaperiodat

76 ation by tunicamycin treatment resulted in a nonglycosylated hOAT4, which was unable to target to the

77 bles into homo and hetero complexes with its nonglycosylated homologue, Rom-1, to maintain the integr

78 covalent homo- and hetero-tetramers with its nonglycosylated homologue, Rom-1.

79 centration of tunicamycin yielded completely nonglycosylated hPTH/PTHrP receptor (approximately 60 kD

80 The nonglycosylated hPTH/PTHrP receptor remains fully functi

81 gh molecular weight (approximately 190 kDa), nonglycosylated hUG-binding protein, readily detectable

82 ycosylated, indicating that glycosylated and nonglycosylated human calreticulin have indistinguishabl

83 Production of two recombinant nonglycosylated human serum transferrins (hTF-NG), conta

84 eir sensitivities to neighboring proline and nonglycosylated hydroxyamino acid residues, from which a

85 fter ATG5 knockdown, while expression of the nonglycosylated IE62 tegument protein was unchanged.

86 y size is sufficient to accommodate a single nonglycosylated Ig domain such as the CD3epsilon ectodom

87 High levels of nonglycosylated IgA were found in patients with Wiskott-

88 The results suggest that the unbound nonglycosylated IL-7Ralpha samples an extensive conforma

89 (1) approximately 10(2) M(-1) s(-1)) for the nonglycosylated interaction to a "diffusion-controlled"

90 d here suggests that the decreased number of nonglycosylated K(2P)3.1 channels on the cell surface ma

91 The nonglycosylated Kv1.2 protein had a similar protein half

92 It is a non-ATP-binding nonglycosylated ligand-regulated molecular chaperone of

93 phate (Man-6-P) modified proteins as well as nonglycosylated ligands.

94 and decreased cellulose interaction for the nonglycosylated linker.

95 7/IL-24 signal peptide linked to the mutated nonglycosylated mda-7/IL-24 gene retained the ability to

96 Vaccination with the nonglycosylated, milk-derived MSP1(42) successfully prot

97 tant thyroglobulin, whereas degradation of a nonglycosylated misfolded secretory protein substrate ap

98 expressed MUC1 have not been successful when nonglycosylated MUC1 sequences were used for vaccination

99 s studies have localized these properties to nonglycosylated mucin domains, and we have recently show

100 ype vesicular stomatitis virus G protein and nonglycosylated mutant forms in the presence of microsom

101 In serial propagation with a nonglycosylated mutant PrP(C) substrate, the dominant Pr

102 allergens of Phl p 4 were expressed, and the nonglycosylated mutant was crystallized.

103 rsor of asialoglycoprotein receptor H2a, its nonglycosylated mutant, makes use of calnexin, EDEM1, an

104 trioxacarcins appear to be derivatives of a nonglycosylated natural product known as DC-45-A2.

105 lecular mass (approximately 90-110 kDa) than nonglycosylated NIS (approximately 50 kDa).

106 ilar affinities; however, the double mutant, nonglycosylated NK1R showed only half the B(max) of the

107 sing a variety of antibodies, reveal a novel nonglycosylated nucleoporin, Nup84, that is tightly asso

108 e ON from E. coli and mammalian cell-derived nonglycosylated ON bind identically to type V collagen a

109 the peptide backbone specific for either the nonglycosylated or the glycosylated peptide.

110 gococci by a novel target recognition of two nonglycosylated outer membrane proteins, Opa and PorB.

111 ced at the cell surface, indicating that the nonglycosylated P2X2 receptors are retained inside the c

112 tion of a collagen triple helix, whereas the nonglycosylated peptide does not.

113 Nonglycosylated peptide fragments were susceptible to co

114 ated vaccine gave CTLs which recognized only nonglycosylated peptide.

115 e reactive with antibody than the synthetic (nonglycosylated) peptide, and periodate treatment of the

116 al sequences of many of the glycosylated and nonglycosylated peptides combined with published informa

117 Nonglycosylated peptides incidentally collected in the s

118 the column and separated from the remaining nonglycosylated peptides present in the digest.

119 Further quantitation of nonglycosylated peptides revealed that sialylation is in

120 copeptides from mixtures of glycosylated and nonglycosylated peptides, sequence both glycan and pepti

121 CTLs, which recognized both glycosylated and nonglycosylated peptides, whereas a similar nonglycosyla

122 ctural similarities between glycosylated and nonglycosylated peptides.

123 cosylation sites on-resin after synthesis of nonglycosylated peptides.

124 Furthermore, the nonglycosylated phytocyanin-like domain from GhPLA1, whi

125 rmed that the expression of glycosylated and nonglycosylated polypeptides occurred in appropriate sub

126 hydroceramide, sphingosine, only ceramide, a nonglycosylated precursor metabolite unable to bind to G

127 The retrotranslocation kinetics of nonglycosylated pro-alpha factor were not significantly

128 o antagonizes the proteasomal degradation of nonglycosylated pro-alpha factor, a postulated nonubiqui

129 HF were incubated with or without exogenous nonglycosylated proBNP1-108 tagged with 6 C-terminal His

130 major component is the 37-kDa, palmitylated, nonglycosylated product of the F13L gene.

131 Furthermore, only low amounts of the nonglycosylated products of the GPEET and EP2 genes were

132 had no effect on the rate of secretion of a nonglycosylated protein (human growth hormone [hGH]) tha

133 ted GLAST increased with maturation, whereas nonglycosylated protein decreased in abundance postnatal

134 We also find that OS-9 binds to a misfolded nonglycosylated protein destined for ERAD, but not to th

135 The native 17-kDa nonglycosylated protein inhibits both human and guinea p

136 Cell-free translation produced a nonglycosylated protein migrating at 29 kDa.

137 vel beta(2)-microglobulin-independent 37-kDa nonglycosylated protein on intestinal epithelia.

138 ted in activated T cells and gives rise to a nonglycosylated protein that resides in the cytosol.

139 his study, we showed that this intracellular nonglycosylated protein was as effective as wild-type MD

140 Sf9 system, homogeneous, crystallizable, and nonglycosylated protein was expressed after mutagenizing

141 h structure by 3.1 kcal/mol, relative to the nonglycosylated protein.

142 its dimeric form, and Epl1 is produced as a nonglycosylated protein.

143 Here, we studied three nonglycosylated proteins and analyzed their use of known

144 somal inhibition induced accumulation of the nonglycosylated proteins and ERAD machinery in the endop

145 Calreticulin also inhibits precipitation of nonglycosylated proteins and thus contains generic prote

146 Mechanisms of targeting of nonglycosylated proteins are less clear.

147 t a shared ERAD pathway for glycosylated and nonglycosylated proteins composed of luminal lectin mach

148 measurements indicate that glycosylated and nonglycosylated proteins induce "open" and "closed" P-do

149 how that calreticulin binds glycosylated and nonglycosylated proteins with similar affinities but dis

150 regation and degradation of glycosylated and nonglycosylated proteins, and antibodies under various c

151 The primary membrane contains nonglycosylated proteins, appears as crescents in the cy

152 side of its mannosidase-like domain with the nonglycosylated proteins.

153 virtually identical with those observed with nonglycosylated pseudocathepsin D.

154 Using glycosylated and nonglycosylated purified flagellin and a cell reporter s

155 asigin, it was demonstrated that the native, nonglycosylated rBSG protein forms dimers in solution.

156 he band representing the photo-cross-linked, nonglycosylated receptor (obtained from the tunicamycin-

157 n enter the (a) "nuclear pathway," where the nonglycosylated receptor is extruded from the pre-Golgi

158 y for human IL-7 300-fold versus that of the nonglycosylated receptor through an allosteric mechanism

159 roduced on expression in transfected cells a nonglycosylated receptor with ligand binding affinity an

160 In contrast, the nonglycosylated receptor, in which all four glycosylatio

161 photoaffinity cross-link specifically to the nonglycosylated receptor.

162 The nonglycosylated receptors displayed normal function and

163 rescence revealed that the expression of the nonglycosylated receptors produced by either tunicamycin

164 lse-positive test results up to 2 kUA/L with nonglycosylated recombinant allergens in patients with h

165 Two forms of nonglycosylated recombinant alpha2AP were expressed, iso

166 A nonglycosylated recombinant ETSHR protein expressed earl

167 Because nonglycosylated recombinant FGF-5 does not appear to be

168 The production of a nonglycosylated recombinant form of Amb a 11 in Escheric

169 Natively glycosylated and nonglycosylated recombinant forms, lenograstim and filgr

170 Glycosylated and nonglycosylated recombinant Phl p 4 showed identical inh

171 M but only weakly inhibiting hydrolysis of a nonglycosylated recombinant protein substrate.

172 ly, these were all confined to two conserved nonglycosylated regions toward the carboxyl terminus of

173 NinaA associates with nonglycosylated Rh1(N20I); therefore, retention of nongl

174 thesized, glycosylated rhodopsin from mature nonglycosylated rhodopsin to characterize the fate of Aa

175 cosylated Rh1(N20I); therefore, retention of nonglycosylated rhodopsin within the ER is not due to th

176 levated concentration of ERManI, whereas the nonglycosylated secretory glycoprotein albumin was not a

177 tional high sensitivities to the presence of nonglycosylated Ser/Thr residues.

178 imultaneous analysis of the glycosylated and nonglycosylated species, we interrogate the hormone bios

179 omomultimers reside in core-glycosylated and nonglycosylated states.

180 culture medium ( approximately 40 mg/L), no nonglycosylated sTFR could be produced, suggesting that

181 of a glycosylated subunit, gp91(phox), and a nonglycosylated subunit, p22(phox).

182 is consistent with a failure of less stable, nonglycosylated subunits to replenish surface compartmen

183 nged in the mutant, suggesting that residual nonglycosylated surface hNETs execute a key step in the

184 ne regions that are related to transporters, nonglycosylated SVOP and highly glycosylated SV2, sugges

185 nt gp36 reduced the antibody reactivity, and nonglycosylated synthetic peptide repeat units from E. c

186 fic B*3501-restricted CTL clone recognized a nonglycosylated, synthetic nonamer representing the mini

187 -restricted, env peptide is identical with a nonglycosylated, synthetic nonamer.

188 to be active at 10-fold lower doses than the nonglycosylated Thr10 contulakin-G analog.

189 e binding affinities of contulakin-G and the nonglycosylated Thr10 contulakin-G for a number of neuro

190 The binding affinity of the nonglycosylated Thr10 contulakin-G was approximately an

191 However, both contulakin-G and nonglycosylated Thr10 contulakin-G were found to be pote

192 dly, Grp170 also promotes degradation of the nonglycosylated transthyretin (TTR) D18G misfolded clien

193 -type NIS activity, including the completely nonglycosylated triple mutant.

194 The expressed protein is nonglycosylated, unlike the protein isolated from the or

195 The nonglycosylated UT-A1 showed reduced localization in lip

196 Wild-type and nonglycosylated V2R yielded tritium signals at 45-55 and

197 nonglycosylated peptides, whereas a similar nonglycosylated vaccine gave CTLs which recognized only

198 Mn(II), Mn(III), and Mn(IV)) and producing a nonglycosylated variant for detailed biochemical and spe

199 enovirus vector expressing a nonsecreted and nonglycosylated version of MDA-7/IL-24 protein was gener

200 urthermore, the glycosylation content (i.e., nonglycosylated vs glycosylated, sialylated vs desialyla

201 from saliva of various races and genders is nonglycosylated with the same molecular weight of 55,881

202 257Y, presumed to be toxic to the cells, and nonglycosylated WT opsin obtained by growth in the prese

203 ith tunicamycin, consistent with the size of nonglycosylated Xenopus laevis alphaENaC.

204 tivity assay conditions, we demonstrate that nonglycosylated XXT2 lacking its cytosolic amino-termina

205 glycosylated COS7-expressed receptor and the nonglycosylated yeast-produced receptor.