ライフサイエンスコーパス: nonionic detergent

1 d with immature HIV-1 particles treated with nonionic detergent.

2 The PDE4D3 form was readily solubilized with nonionic detergents.

3 ivalent ion requirements, effect of salt and nonionic detergents.

4 und proteins is performed in the presence of nonionic detergents.

5 fraction by high salt concentrations, or by nonionic detergents.

6 urea or sodium dodecyl sulfate (SDS) but not nonionic detergents.

7 C-MMOH complex is perturbed by salts but not nonionic detergents.

8 ngth but is nearly unaffected by addition of nonionic detergents.

9 ions of Triton X100, Nonidet P40 and Brij-58 nonionic detergents.

10 n Stat5bA630P in cell extracts prepared with nonionic detergents.

11 egions that are resistant to extraction with nonionic detergents.

12 ies of straight chain anionic, cationic, and nonionic detergents.

13 ains can be extracted differentially by cold nonionic detergents.

14 sucrose density gradients and are stable in nonionic detergents.

15 ere readily dissociable upon the addition of nonionic detergent (0.1% Triton X-100).

16 y pure systems that contain a bile salt or a nonionic detergent, a phosphatidylcholine or a fatty aci

17 nd resistance of E-cadherin to extraction by nonionic detergents, a measure for the association of th

18 Treatment of the extracts with nonionic detergents, a membrane-altering inhibitor of fa

19 cause a fraction of DAH remains insoluble to nonionic detergent along with actin.

20 Nonionic detergents alter the Bax conformation to expose

21 measured by resistance to solubilization in nonionic detergent and by copatching with a raft-residen

22 be induced to deflagellate by treatment with nonionic detergent and Ca2+.

23 etermined crystal structure solubilized by a nonionic detergent and complexed with an antibody fragme

24 The enzyme requires a nonionic detergent and divalent metal ions for activity.

25 scopic scale measured as lower solubility in nonionic detergent and in the microscopic scale evident

26 d was partially released by treatment with a nonionic detergent and reducing agent, consistent with a

27 ion of mature virions after treatment with a nonionic detergent and reducing agent.

28 fied virions occurred after treatment with a nonionic detergent and reducing agent.

29 rmined biochemically using solubilization in nonionic detergents and by confocal microscopy.

30 ter-soluble organic dyes are also soluble in nonionic detergents and can be extracted by adding salt,

31 p235 PI 5-kinase include high sensitivity to nonionic detergents and relative resistance to wortmanni

32 stable at 4 degrees C in buffers containing nonionic detergents and showed a redox midpoint potentia

33 luble molecule to one that aggregated, bound nonionic detergent, and bound to lipid vesicles, propert

34 om up to 1 ml of plasma, gently lysed with a nonionic detergent, and directly amplified.

35 ne tag was overexpressed, solubilized with a nonionic detergent, and purified by nickel affinity chro

36 embrane vesicles, it can be solubilized with nonionic detergents, and it shifts from 220 to 200 kDa u

37 ely co-immunoprecipitated with p59(fyn) from nonionic detergent (Brij 96) extracts of early postnatal

38 It was solubilized in a nonionic detergent buffer, enriched by differential cent

39 hese differences, each mutant was soluble in nonionic detergent but unable to assemble into homomeric

40 ion of the Ca-ATPase into monomers using the nonionic detergent C(12)E(8) gave a pattern of phosphory

41 s (bilayer interfacial area), inclusion of a nonionic detergent (C(12)E(8)), and the presence of chol

42 mild extraction of plasma membranes with the nonionic detergent C12E8 (octaethylene glycol n-dodecyl

43 Treatment of nb/nb ghosts with the nonionic detergent C12E8 (octaethylene glycol n-dodecyl

44 We have studied the effects of the nonionic detergent C12E8 on Ca-ATPase enzymatic activity

45 ive to the presence of contaminants, such as nonionic detergents commonly found in biological samples

46 ich dissociates and loses kinase activity in nonionic detergent conditions.

47 of mannose 6-phosphate or octyl glucoside, a nonionic detergent containing a sugar group, to cocultur

48 viral cores from particles treated with mild nonionic detergent; cores were isolated by sedimentation

49 When fresh erythrocytes are solubilized by nonionic detergent, CR1 partitions to the cytoskeleton f

50 Examination of virions after treatment with nonionic detergent demonstrated that: (i) in extracellul

51 column eluate after displacement of SDS with nonionic detergent, demonstrated by gel filtration and c

52 It was found that nonionic detergents did not dissociate the polymers, but

53 The nonionic detergent, digitonin, and the anionic detergent

54 oli and solubilized from cell lysates in the nonionic detergent, dodecyl maltoside.

55 In addition, we establish with both the nonionic detergent dodecylmaltoside and the anionic dete

56 The nonionic detergent dodecylmaltoside solubilized function

57 ssed in Sf9 insect cells and stabilized with nonionic detergents during purification.

58 The receptors are resistant to extraction by nonionic detergent even after latrunculin A treatment.

59 brain, it was immunoaffinity-purified from a nonionic detergent extract of washed mouse brain membran

60 Lipid rafts were isolated after cold, nonionic detergent extraction of cells and gradient cent

61 Nonionic detergent extraction of cultured epithelial cel

62 urified virions and was largely resistant to nonionic detergent extraction, suggesting a location wit

63 supported monolayers and remains bound after nonionic detergent extraction.

64 oimmunoprecipitates with beta1-integrin from nonionic detergent extracts and colocalizes with vinculi

65 In nonionic detergent extracts from MDCK II cells, the tigh

66 gh the extraction of the outer membrane with nonionic detergent followed by ion-exchange chromatograp

67 e-exposure to 4-nonylphenol (4-NP), a common nonionic detergent found in sewage sludge amended soils.

68 ng monospecific anti-IAP100 antibodies and a nonionic detergent-generated chloroplast lysate gave the

69 In the absence of nonionic detergents, gp140 of the KNH1144 genotype, term

70 However, nonionic detergents have been shown to cause signal supp

71 ucrose gradients nor decreased solubility in nonionic detergents-hence it does not promote lipid raft

72 ents or heat but are relatively resistant to nonionic detergents, high salt concentrations, or exposu

73 nant by treating the stock suspension with a nonionic detergent, Igepal CA-630.

74 dylinositol 4-kinase (PI 4-K) (stimulated by nonionic detergent, inhibited by adenosine, inhibited by

75 sulted in elevated FAK.Cas complex levels in nonionic detergent-insoluble fractions, indicating incre

76 s, we analyzed whether they partitioned into nonionic detergent-insoluble glycolipid-enriched membran

77 e chain oxidation is observed for TBP when a nonionic detergent is present.

78 ightly membrane bound, and its inhibition by nonionic detergents is described.

79 A detailed list of compatible nonionic detergents is included, along with a protocol f

80 s stimulated to a much greater extent by the nonionic detergent lauryldimethylamine oxide (LDAO) than

81 r to the fidelity when using IN derived from nonionic detergent lysates of HIV-1 virions.

82 he SC-TR assay is compatible with the use of nonionic detergents, making it more versatile than other

83 ganic dyes that uses a reagent composed of a nonionic detergent mixed with an alcohol is described.

84 ively modulating TATA box binding by TBP and nonionic detergent modulating the interdomain interactio

85 The nonionic detergent n-dodecyl-beta-D-maltopyranoside is c

86 synaptosomal and microsomal membranes by the nonionic detergent n-octyl- beta-glucopyranoside; the so

87 etergent sodium dodecyl sulfate (SDS) or the nonionic detergent n-octyl-beta-D-glucopyranoside (betaO

88 ted membranes and membranes treated with the nonionic detergent n-octyl-beta-d-glucopyranoside, sugge

89 protein was monodisperse and dimeric in the nonionic detergent n-octyl-beta-D-glucopyranoside.

90 by extracting pig brain microsomes with the nonionic detergent Nonidet P-40 and purified approximate

91 is strongly promoted by molybdate and by the nonionic detergent Nonidet P-40.

92 Next, the nonionic detergent, Nonidet P-40, was used to extract th

93 Treatment of intact organisms with the nonionic detergent NP-40 resulted in dissolution of the

94 It was extracted from the membrane by the nonionic detergent NP-40, together with glycerophospholi

95 ffer containing oxidized glutathione and the nonionic detergent octyl glucoside, the G protein regain

96 Escherichia coli polar lipid extract and the nonionic detergent octyl-beta-d-glucopyranoside.

97 The nonionic detergent octyl-beta-glucoside, which does not

98 The effect of nonionic detergents of the n-alkyl-beta-D-glucopyranosid

99 d purified using ionic detergent, LDS-P5, or nonionic detergent, OG-P5.

100 The effects of the ionic and nonionic detergents on catalytic activity of endometase

101 Cell extraction with cold nonionic detergents or alkaline carbonate prepares an in

102 f bands around 27 kDa, and extractions using nonionic detergents or high pH conditions demonstrate th

103 hrome c oxidase (CcO), solubilized by either nonionic detergents or phospholipids, completely dimeriz

104 ing a multiwell detergent screening assay, 4 nonionic detergents out of 80 tested were found to dispe

105 and extracted in buffer without or with the nonionic detergent polidocanol.

106 The nonionic detergent polyoxyethylene 10 lauryl ether (C12E

107 On the other hand, nonionic detergents readily induced homodimers and heter

108 ntrolled manner with various combinations of nonionic detergents, reducing agents, and proteolytic en

109 ane-associated Type I ROPs display increased nonionic detergent solubility in pan1 mutants, suggestin

110 a in two distinct intracellular pools (i.e., nonionic detergent-soluble and detergent-insoluble pools

111 in most detergents and can be maintained in nonionic detergent solutions for extended periods of tim

112 eport, we present evidence that NS4A forms a nonionic-detergent-stable complex with the NS4B5A polypr

113 MALDI-TOF can be obtained in the presence of nonionic detergents such as hydrogenated Triton X-100 (R

114 s are characterized by their insolubility in nonionic detergents such as Triton X-100 at 4 degrees C.

115 Nonionic detergents such as Triton X-100, Nonidet P-40 a

116 purified virions and could be extracted with nonionic detergent, suggesting membrane insertion.

117 Caspr is poorly extracted by nonionic detergents, suggesting that it is associated wi

118 the critical micelle concentration (CMC) of nonionic detergents tested.

119 within certain temperature ranges, or other nonionic detergents than bilayers in the fluid phase.

120 by octyl-beta-glucoside, the latter being a nonionic detergent that selectively extracts only band 3

121 The nonionic detergents that are typically used to achieve s

122 In the presence of nonionic detergent, the 6A7 antibody avidly binds the mo

123 ared by freeze-thawing or by the addition of nonionic detergent, the Vn antigen content was drastical

124 uantify the effects that a popular series of nonionic detergents, the n-alkyl-beta-D-glucopyranosides

125 Addition of a nonionic detergent to CVM caused the average pore size t

126 subfraction of particulate PIKfyve resisted nonionic detergent treatment, implying association with

127 of the membrane by low concentrations of the nonionic detergent Triton X-100 (0.3%) or the mild ionic

128 n the presence of a low concentration of the nonionic detergent Triton X-100, specific pyrethroid bin

129 omplex was not altered by treatment with the nonionic detergent Triton X-100, suggesting a lack of as

130 lyzed by immunostaining with and without the nonionic detergent Triton X-100, using the CD44H monoclo

131 tion of M. tuberculosis H37Ra lysates by the nonionic detergent Triton X-114 revealed the Eis protein

132 strategy based on micelle formation with the nonionic detergent Triton X-114.

133 The nonionic detergents Triton X-100, Nonidet P-40, Brij, Tw

134 ells and was extracted from the membranes by nonionic detergents (Triton X-100, dodecyl maltoside).

135 The presence of a nonionic detergent, Triton X-100, was found essential to

136 ins extracted from the viral membrane with a nonionic detergent were shown to conserve the a-determin

137 m to which various combinations of ionic and nonionic detergents were admixed.

138 Ionic and nonionic detergents were inhibitory for each enzyme.

139 DeltaE-torsin A were readily solubilized by nonionic detergents, were similarly accessible to protea

140 , Duffy, XK, and Kell readily extractable by nonionic detergent with no effect on the retention of ba

141 We have also found that mixing nonionic detergents with alcohols markedly reduces their

142 e outer membrane complex by a combination of nonionic detergents with reducing agents but not by the