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1 ses (PKSs), Hpm8 (highly reducing) and Hpm3 (nonreducing).
3 species of approximately 120 kDa under both nonreducing and reducing conditions on SDS-polyacrylamid
4 ed to the carboxylates of the I rings at the nonreducing and reducing ends of the IAI trisaccharide s
5 - and tri-Manp motifs that functionalize the nonreducing arabinan termini of LAM (ManLAM) in Mycobact
6 presence of but a single Manp residue on the nonreducing arabinan termini of LAM and also a complete
7 n further be separated into two subclasses: "nonreducing" ARO/CYCs, which act on nonreduced poly-beta
9 ized by their ability to hydrolyze terminal, nonreducing beta-D-galactosyl residues from beta-D-galac
12 alpha-L-Rhap(1-->2)alpha-L-Rhap motif at its nonreducing chain terminus, stressing the importance of
14 e and can be performed in either reducing or nonreducing conditions (e.g., to preserve disulfide bond
15 raphic purification strategy performed under nonreducing conditions (to maintain cysteine disulfide s
16 he apparent molecular mass was 136 kDa under nonreducing conditions and 34 kDa under reducing conditi
17 protein bands of approximately 66 kDa under nonreducing conditions and a single 25 kDa band under re
18 Velocity sedimentation under reducing- and nonreducing conditions and co-immunoprecipitation experi
19 we prepared isolated bovine complex I under nonreducing conditions and employed the techniques of ma
20 e disulfide bond modification occurred under nonreducing conditions and was not an artifact of sample
21 , and was detectable under both reducing and nonreducing conditions as a 28-kD protein in BALF from t
22 ly bound approximately 55-kDa molecule under nonreducing conditions but dissociates under reducing co
23 lysis of H120C/H213C protein separated under nonreducing conditions demonstrated that the ectopic bon
24 to determine a DeltaGu value for E2cd under nonreducing conditions due to problems with reversibilit
25 s observed using SDS-PAGE under reducing and nonreducing conditions for bacterially expressed and mam
26 ential reactivity of 6-CP under reducing and nonreducing conditions highlights the ability of radical
27 leads to "explosive" fibrillation only under nonreducing conditions implying the key role of the disu
28 kDa under reducing conditions, whereas under nonreducing conditions it presented multimeric assembly
30 s was similar between genotypes, EMSAs under nonreducing conditions showed increased DNA binding in p
31 tern analysis of cell-surface proteins under nonreducing conditions showed that the CaR exists in sev
32 wed by Western blotting after SDS-PAGE under nonreducing conditions suggested that several SIRP prote
35 acid 330 produced a monomer-sized band under nonreducing conditions which was converted upon reductio
37 ulfate-polyacrylamide gel electrophoresis in nonreducing conditions, and electron microscopy revealed
38 tion by endoproteinase Lys-C performed under nonreducing conditions, as well as tandem MS experiments
42 SDS-polyacrylamide gel electrophoresis under nonreducing conditions, it was found that an intermolecu
46 tially more stable than wild-type IPK1 under nonreducing conditions, revealing that increased stabili
47 ormed high molecular weight aggregates under nonreducing conditions, suggesting an increased propensi
51 rP(Sc)-like form could be accomplished under nonreducing conditions, without the need to disrupt the
73 f 3,3'-neotrehalosadiamine (NTD), an unusual nonreducing disaccharide reported to have antibiotic pro
74 dependent induced morphogenesis, whereby the nonreducing disaccharide trehalose acts as a negative re
75 acial binding sites that can accommodate the nonreducing disaccharides is key for their strong impact
78 nant forms of micro 1 seen in gels after the nonreducing disruption of virions are ds-linked dimers.
79 ide evidence that NS domains residing at the nonreducing end (NRE) are, on average, longer than those
80 of the terminal trisaccharide, having at the nonreducing end a GlcNAc or GalNAc, and bound them to BS
81 ing N-acyl and O-acetyl modifications at the nonreducing end and a critical 6-O-sulfate at the reduci
82 igosaccharides containing a galactose at the nonreducing end and a propargyl group at the reducing en
83 ccharides of N-glycan with a GlcNAc at their nonreducing end and found that the extended sugar moiety
84 ontaining an unsaturated uronate unit at the nonreducing end and two contiguous AT-binding sequences
85 binding subsites (-I, -II, and -III) for the nonreducing end and two glucose binding subsites (+I and
89 >/=1/10,000), but only vaccines enriched for nonreducing end de-N-acetyl residues by treatment with e
90 erminal 4,5-unsaturated glucuronic acid, the nonreducing end disaccharide moiety does not interact wi
92 also confirming that growth occurred at the nonreducing end following initiation on phosphatidylglyc
94 h the azido group at the C-6 position of the nonreducing end fucose could elicit a strong IgG immune
95 d alpha2-3- or alpha2-6-linked to a terminal nonreducing end galactose, poly-LacNAc extended core-3 O
96 plex with an oligosaccharide acceptor with a nonreducing end GlcNAc that has a beta1-6-glycosidic lin
98 sthydrolysis state in which the newly formed nonreducing end has already left the substrate binding p
100 and masked as a 1,6-anhydro sugar, while the nonreducing end is activated as a free alkyne and masked
101 s contain neither the galactose at the Le(a) nonreducing end nor the fucose at the Le(x) reducing end
102 ohydrate-binding module (CBM) that binds the nonreducing end of beta-1,3-glucan chains, and an unchar
104 ution reveals that the GlcNAc residue at the nonreducing end of chitobiose makes extensive hydrophobi
105 n (GH18(C)) and releases chitobiose from the nonreducing end of chitooligosaccharides, therefore func
107 samines present as monosaccharides or at the nonreducing end of odd-numbered oligosaccharide substrat
108 ronate or beta-D-manuronate residues, at the nonreducing end of oligo-alginates in an exolytic fashio
109 the unsaturated Delta4,5 uronic acid at the nonreducing end of oligosaccharides resulting from prior
113 ucing an azido-containing sialic acid to the nonreducing end of the galactosides through a sialyltran
115 lternate additions of Glc and GlcUA onto the nonreducing end of the growing polysaccharide chain.
116 l" antithrombin binding site and also at the nonreducing end of the molecule, which is reported in in
119 luronic acid blocks from the reducing to the nonreducing end of the polymer following the initial ran
121 4, where the (GlcNAc)2 group arises from the nonreducing end of the substrate and is formed as the be
126 meric enzyme that releases fructose from the nonreducing end of various oligosaccharides and essentia
127 ith its C-terminus free and the GPI with its nonreducing end phosphoethanolamine bearing a free amino
130 and (GlcNAc)4 where dimers cleaved from the nonreducing end reflect the most common binding and hydr
131 accumulated heparan sulfate exhibits unique nonreducing end structures with terminal N-sulfoglucosam
132 n bonding between Asn(462) and xylose at the nonreducing end subsite +2 was important for the higher
133 at 355 nm, producing fragment ions from the nonreducing end that do not contain the appended fluorop
136 The enzyme sulfates the substrate from the nonreducing end toward the reducing end consecutively, l
137 hin sulfate (ECS), was designed to mimic the nonreducing end trisaccharide unit DEF of the sequence s
139 alogues with modification at the reducing or nonreducing end were synthesized using chemoenzymatic me
140 hand, synthesis from the reducing end to the nonreducing end yielded the mannopentaose with the desir
142 1 at the residues immediately located at the nonreducing end, allowing the formation of an N-Ac domai
143 stic of neuraminic acid, particularly at the nonreducing end, may be important for processing and pre
144 -O-, 5-O- and 3,5-di-O-linked alpha-Araf and nonreducing end-units of alpha-Araf, Arap, beta-Galp and
157 of glycosyl hydrolases (GH5) and by Cel6A, a nonreducing-end cellobiohydrolase from family GH6 with t
158 n and minimally to an altered binding of the nonreducing-end DEF trisaccharide to the native serpin c
159 oside hydrolase Family 6 (GH6) CBHs act from nonreducing ends by an inverting mechanism and are prese
160 results suggest that glycosidases acting on nonreducing ends digest large amounts of xyloglucan in w
161 type that could be explained by an excess of nonreducing ends leading to a reinforced xyloglucan netw
162 by W58L was confirmed from both reducing and nonreducing ends of CNP-labeled oligosaccharide substrat
165 and 2-O-sulfo-iduronic acid residues at the nonreducing ends of the glycans as co-receptors for Shh.
168 gosaccharides with the sulfate esters at the nonreducing ends preferentially bind to the lectin.
169 ed cellodextrins modified at the reducing or nonreducing ends upon incubation with cellulose and cell
170 harides contained N-acetylglucosamine at the nonreducing ends, galactosylation was judged to be ineff
174 ylated and its glycan antennae bear terminal nonreducing galactosyl residues in alpha1-3 linkage.
176 immunoblotting profile of the IgA using a 6% nonreducing gel verified the dimeric nature of the autoa
180 FAbs reacted with soluble E2 protein only in nonreducing gels, indicating that the relevant epitopes
182 und having a galactosyl moiety at C-4 of the nonreducing GlcNAc moiety bound in the low micromolar ra
185 trons to the heterodisulfide-H(2) via F(420)-nonreducing hydrogenase or formate via formate dehydroge
186 se, formylmethanofuran dehydrogenase, F(420)-nonreducing hydrogenase, and formate dehydrogenase.
188 with product that is further elaborated by a nonreducing iPKS (nrPKS) to yield a 1,3-benzenediol moie
189 dc5, the highly reducing IPKS, and Rdc1, the nonreducing IPKS, are required for the biosynthesis of r
191 ucing iterative PKS, Hpm8, cooperates with a nonreducing iterative PKS, Hpm3, to construct the advanc
193 SAT domains in starter unit selection among nonreducing iterative, fungal PKSs, and they provide a b
194 ynthetic versatility of TE domains in fungal nonreducing, iterative PKSs (NR-PKSs) has been shown to
196 ) generated by FAD reduction relative to the nonreducing ligands demonstrates that FAD reduction cont
198 ined the localization of N-glyans containing nonreducing mannosyl groups, accentuating the GNA vesicu
199 l variability of aromatic products of fungal nonreducing, multidomain iterative polyketide synthases
200 a combination of CNBr and chymotrypsin under nonreducing, nonalkylating conditions in combination wit
201 e tight binding of the nascent chain via its nonreducing oligosaccharide terminal segment to the carb
203 ution of Gibberella zeae PKS13, which is the nonreducing PKS associated with zearalenone biosynthesis
204 th a sequential and convergent manner with a nonreducing PKS to form the chaetomugilin and chaetoviri
205 cture analysis of a group of related fungal, nonreducing PKSs has revealed well defined N-terminal do
206 we have replaced, en masse, the promoters of nonreducing polyketide synthase (NR-PKS) genes, key gene
208 form the tropolone nucleus: tropA encodes a nonreducing polyketide synthase which releases 3-methylo
210 Product template (PT) domains from fungal nonreducing polyketide synthases (NR-PKSs) are responsib
212 und production and release from six of these nonreducing polyketide synthases and have identified the
213 We have validated this system by expressing nonreducing polyketide synthases of Aspergillus terreus
214 ctroscopy demonstrated that D239T could bind nonreducing pyridine nucleotides with a phosphate or a h
216 acts on a YajL affinity column, separated by nonreducing-reducing SDS-PAGE, and identified by mass sp
217 ntional two-dimensional electrophoresis: (i) nonreducing/reducing or (ii) isoelectric focusing/reduci
218 d to various oxidative insults by sequential nonreducing/reducing two-dimensional SDS-PAGE combined w
222 he appearance of disulfide-bonded species on nonreducing SDS--PAGE and protection of 4-OHEN-mediated
223 acid resulted in two radioactive species on nonreducing SDS-PAGE gels, with molecular weights corres
224 ng proteolytic enzymes are first resolved in nonreducing SDS-PAGE on a gel without protein substrate.
226 s and Western blots of proteins subjected to nonreducing SDS-PAGE suggested that all four 1F5 scFv-Ig
228 of Forster resonance energy transfer (FRET), nonreducing SDS-PAGE, and strategic mutation of the Ab h
229 sulins exhibit abnormally slow mobility upon nonreducing SDS-PAGE, despite normal mobility upon reduc
230 g occurs as a discrete "jump" in mobility by nonreducing SDS-PAGE, suggesting formation of at most a
231 mass heterogeneity following reducing versus nonreducing SDS-PAGE, we determined that the beta-subuni
235 rated at about 47.5 kDa on both reducing and nonreducing SDS-polyacrylamide gel electrophoresis, indi
236 te as high molecular weight aggregates under nonreducing SDS-polyacrylamide gel electrophoresis, sugg
237 nd with resulting downward mobility shift on nonreducing SDS-polyacrylamide gel electrophoresis.
238 the apparent molecular mass determined with nonreducing SDS/PAGE from approximately 85 to approximat
241 ondroitin sulfation at the 4-position on the nonreducing site of the linkage to be cleaved or 2-sulfa
242 inhibition, and analysis of band patterns on nonreducing sodium dodecyl sulfate--polyacrylamide gel e
243 hilized, and subjected to electrophoresis on nonreducing sodium dodecyl sulfate-5% polyacrylamide gel
244 ovel VirB7 and VirB9 complexes detectable by nonreducing sodium dodecyl sulfate-polyacrylamide gel el
245 lar disulfide linkages that were revealed by nonreducing sodium dodecyl sulfate-polyacrylamide gel el
246 oresis of protease-containing samples into a nonreducing sodium dodecyl sulfate-polyacrylamide gel el
247 s, along with an in-depth comparison, of the nonreducing (StfQ) and reducing (BexL) di-domain ARO/CYC
248 sugars without the interferences from their nonreducing structural isomers in mouse heart tissue, wi
249 at m/z 221 anions are comprised of an intact nonreducing sugar glycosidically linked to a 2-carbon ag
256 These exclusive interactions between the nonreducing sugars and the membranes may rationalize why
257 , which preferentially binds to a cluster of nonreducing terminal alpha1,3-linked mannosyl residues,
258 rom additional favorable interactions of the nonreducing terminal galactose, as well as of the fucose
259 ulfotransferases can transfer sulfate to the nonreducing terminal GlcNAc of short carbohydrate substr
260 branched, primarily with double branches of nonreducing terminal l-arabinosyl units at the O-2 and O
261 to Ca(2+) in a primary binding site and the nonreducing terminal mannose residue occupying an adjace
262 Here, we evaluated the influence of the nonreducing terminal monosaccharide on the serum antibod
263 proteins are involved in the assembly of the nonreducing terminal motif of arabinogalactan and EmbC i
264 beta1,4-galactosylation is the presence of a nonreducing terminal N-acetylglucosamine; however, this
265 bits highest specificity and affinity toward nonreducing terminal Neu5Acalpha2, 6Galbeta1,4Glc/GlcNAc
267 inding patterns, confirmed the presence of a nonreducing terminal repeating LacNAc ((Galbeta1-->4GlcN
268 onal immunoglobulin A (IgA) specific for the nonreducing terminal residue of Ogawa O-polysaccharide (
269 For IgG1s S-20-4, A-20-6, and IgA 2D6, the nonreducing terminal residue of Ogawa O-PS is the domina
271 The rhamnogalacturonan I has numerous single nonreducing terminal residues of the rare sugar l-galact
273 dase digestion confirmed the identity of the nonreducing terminal sugar of the disaccharide and estab
276 with the antigenic structure located in the nonreducing termini (i.e., Fucalpha1-2Galbeta1-3GalNAc-R
279 oval of individual saccharide units from the nonreducing termini of the multiantennary structures.
280 hain oligosaccharides are substituted at the nonreducing termini with single 4-O-methyl-glucuronosyl
285 at the fragments were consistent with an LTA nonreducing terminus consisting of GalNAc(alpha1-->3)Gal
287 carbohydrate epitope that is present at the nonreducing terminus of sugar chains of glycoproteins an
288 ongation is inhibited by truncation when the nonreducing terminus of the growing chain is capped with
290 rms of Nz28, sequentially truncated from the nonreducing terminus, possess only one PEtn moiety.
291 es in the N-glycan core, once exposed in the nonreducing terminus, was able to serve as acceptors for
296 accharides; alpha- and beta-cyclodextrins; a nonreducing tetrasaccharide, stachyose; an N-acetylamide
298 ed by reciprocal co-immunoprecipitation, and nonreducing velocity sedimentation was used to identify
299 sites residing outside of EC2 as assayed by nonreducing Western blot analysis, sedimentation velocit
300 ed form of wild-type gB was identified under nonreducing Western blot conditions that likely represen
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