ライフサイエンスコーパス: nonsense codon

1 p also enhances translation termination at a nonsense codon.

2 itution in the mutant gene, which produced a nonsense codon.

3 e isomerization of uridine to Psi within the nonsense codon.

4 enerates three missense codons followed by a nonsense codon.

5 nduce skipping of the exon that contains the nonsense codon.

6 n in mammalian cells in response to an amber nonsense codon.

7 two base changes, one of which formed a TGA nonsense codon.

8 he third, it results from homozygosity for a nonsense codon.

9 in Escherichia coli in response to the amber nonsense codon.

10 nslational fidelity in response to the amber nonsense codon.

11 acid into proteins in response to the amber nonsense codon.

12 denylation regardless of the position of the nonsense codon.

13 anslation termination process at a premature nonsense codon.

14 n inactive form because of the presence of a nonsense codon.

15 yrosine into protein in response to an amber nonsense codon.

16 selectively stabilize mRNAs harboring early nonsense codons.

17 ion-independent decapping triggered by early nonsense codons.

18 = any base) with the remaining codons being nonsense codons.

19 ng mRNA decay and translation termination at nonsense codons.

20 vivo and translational recoding of lysine at nonsense codons.

21 acids at specific sites designated by amber nonsense codons.

22 two A-site-specific drugs and on suppression nonsense codons.

23 d involved [PSI(+)]-mediated read-through of nonsense codons.

24 eukaryotic cells to degrade mRNAs containing nonsense codons.

25 ory elements identified that act upstream of nonsense codons.

26 zipper [ZIP]) domains by either deletions or nonsense codons.

27 s the offending mutations that generate such nonsense codons.

28 wn mammalian gene transcripts in response to nonsense codons.

29 functions to degrade transcripts containing nonsense codons.

30 rades mRNAs harboring premature termination (nonsense) codons.

31 e, resulting in translation termination at a nonsense codon 138 nucleotides downstream of the deletio

32 Transcripts with nonsense codons about 20, 40 or 60% of the way through t

33 s genetically encoded in E. coli by an amber nonsense codon and corresponding orthogonal tRNA/aminoac

34 n Saccharomyces cerevisiae by using an amber nonsense codon and corresponding orthogonal tRNA/aminoac

35 reduce the abundance of mRNAs that harbor a nonsense codon and prematurely terminate translation.

36 This mutation changes an AAG codon to a TAG nonsense codon and results in a null phenotype.

37 was previously shown to cause readthrough of nonsense codons and high temperature-conditional lethali

38 vidual amino acids was distinct for specific nonsense codons and readthrough-inducing agents.

39 te tRNAs could mispair at position 1 or 3 of nonsense codons and that, irrespective of whether readth

40 the efficiency of translation termination at nonsense codons and/or the process of programmed -1 ribo

41 tening, arrest of translation at a premature nonsense codon, and endonucleolytic cleavage.

42 trons that normally reside downstream of the nonsense codon are deleted so the nonsense codon is loca

43 mRNAs often contain premature-termination (nonsense) codons as a result of mutations and RNA splici

44 their activity in a FLuc reporter cell-based nonsense codon assay, with PTC124 emerging as the most p

45 unstable mRNAs, whereas a transcript with a nonsense codon at 80% was as stable as wild-type.

46 a protein encoded by a gene with an in-frame nonsense codon at an essential lysine can be expressed i

47 Here, we provide evidence that a nonsense codon at position 1, 2 or 10 reduces the abunda

48 Until now, a nonsense codon at position 23 has been the 5'-most nonse

49 CAT) gene that lacked an initiation codon, a nonsense codon at TPI position 1 or 2 allowed for the pr

50 produced by a nonsense-free gene, whereas a nonsense codon at TPI position 23 precluded the producti

51 ffect was evaluated by introducing premature nonsense codons at different distances from the PHA AUG

52 beta (TCRbeta) gene, which acquires in-frame nonsense codons at high frequency during normal lymphocy

53 ults are consistent with the notion that the nonsense codon can function in the cytoplasm by promotin

54 the particular mRNA and how it is produced, nonsense codons can mediate a reduction in mRNA abundanc

55 These nonsense codons cause rapid decay of the mRNAs that cont

56 markably, we also show that pseudouridylated nonsense codons code for amino acids with similar proper

57 All three translation termination codons, or nonsense codons, contain a uridine residue at the first

58 eased levels of spliced RNA from the unusual nonsense codon-containing U22 host gene, which is a natu

59 1 is required in yeast for discrimination of nonsense-codon-containing mRNA from normal by NMD.

60 d in the stabilization of both wild-type and nonsense-codon-containing mRNAs in vivo.

61 pidly degrade certain transcripts with early nonsense codons could provide plant cells with a means t

62 ng in the substitution of glutamic acid by a nonsense codon, designated E133X.

63 s were replaced by new amino acids that used nonsense codons differing by one base change from the se

64 ide 1101 (epsilon 11O1insT) that generates a nonsense codon directly, and insertion of a guanine afte

65 In support of scanning, a nonsense codon does not elicit decay if some of the intr

66 Here we show that the nonsense codon effect results primarily from lowering th

67 The positional dependence of the nonsense codon effect was evaluated by introducing prema

68 that a surveillance complex searches 3' of a nonsense codon for a downstream sequence element (DSE) a

69 We show that nonsense codons from variable (V) Igkappa exons promote

70 f mutation, the rate of reversion of the TAG nonsense codon has been determined for different motifs

71 s measured by translational suppression of a nonsense codon in a luciferase reporter gene.

72 A), which has been modified to translate the nonsense codon in mcd1-2.

73 he sasA1 mutant, which was found to encode a nonsense codon in the beginning of rfbA, produced less O

74 derivatives were used for suppression of the nonsense codons in a cell-free protein biosynthesizing s

75 cine was employed for the suppression of the nonsense codons in a cell-free protein biosynthesizing s

76 B1 induced readthrough at all three nonsense codons in cultured cancer cells with TP53 (tumo

77 ese hamster ovary (CHO) cell mutants bearing nonsense codons in four of the five exons of the adenine

78 lity of attempts at regulated suppression of nonsense codons in mammalian cells by regulating express

79 acids in response to three independent amber nonsense codons in sperm whale myoglobin or green fluore

80 The present analysis of nonsense codons in the human alpha-globin mRNA illustrat

81 that they are down-regulated in response to nonsense codons in the nuclear fraction of cells.

82 amma2a heavy chain constructs containing TAG nonsense codons in their V regions that are part of eith

83 mulation of transcripts containing premature nonsense codons in transgenic plants.

84 cid residues, site-directed incorporation at nonsense codons in Xenopus laevis oocytes, and electroph

85 d: base substitutions leading to missense or nonsense codons, in-frame deletions or duplications with

86 repression or induction suggested that this nonsense codon-induced partitioning shift (NIPS) respons

87 cteristic of strain C57BL/6Jand introduces a nonsense codon into sodium channel modifier 1 (SCNM1), a

88 Following the introduction of nonsense codons into promoter-proximal genes, polarity i

89 uently added to the genetic code by changing nonsense codons into sense codons for these amino acids.

90 nd aberrant MLH1 transcript encompassing the nonsense codon is also produced.

91 eam of the nonsense codon are deleted so the nonsense codon is located (i) too far away from a downst

92 In vivo targeting of nonsense codons is accomplished by the expression of an

93 only well recognized entity that recognizes nonsense codons is the cytoplasmic translation apparatus

94 s, we demonstrated that the recognition of a nonsense codon led to a decrease in the translational ef

95 In-frame nonsense codons located at a minimum distance upstream o

96 human triosephosphate isomerase (TPI gene), nonsense codons located less than 50 to 55 bp upstream o

97 n-exon junction of product mRNA so that only nonsense codons located more than 50 to 55 nucleotides u

98 ormed cell lines demonstrated that premature nonsense codons markedly destabilized the mRNA.

99 A codon during mRNA translation augments the nonsense-codon-mediated decay of cytoplasmic Se-GPx1 mRN

100 very that 129-derived strains of mice have a nonsense codon mutation in exon 2 that abrogates product

101 1-2, had a single base change resulting in a nonsense codon near the N-terminus.

102 ominant RP1 alleles typically have premature nonsense codons occurring in the last exon of the gene a

103 e phenotypes examined include suppression of nonsense codons on different media and at different temp

104 on the suppression of altered start codons, nonsense codons or frameshift mutations in genes involve

105 region, alternative splicing that introduces nonsense codons or frameshifts, introns in the 3' untran

106 A) anticodon to CUA so as to translate amber nonsense codons permits tRNA (Trp)(CCA) to be aminoacyla

107 ORFs would function in a manner analogous to nonsense codons, promoting rapid degradation of the mRNA

108 nvestigated the therapeutic potential of the nonsense codon read-through-inducing drug, PTC124, in tr

109 strated ability of the [PSI+] prion to cause nonsense-codon read-through.

110 preexisting nonsense suppression facilitated nonsense codon reassignments and constitutes a novel mec

111 tion, we demonstrate a striking link between nonsense codon reassignments and the decoding properties

112 ppressor tRNAs provided the raw material for nonsense codon reassignments, implying that the properti

113 tRNA anticodon have dictated the identity of nonsense codon reassignments.

114 This anomaly depends on prior nonsense codon recognition and is eliminated in extracts

115 ediated mRNA decay (NMD) is a consequence of nonsense codon recognition during a pioneer round of tra

116 accordingly, NMD occurs as a consequence of nonsense codon recognition during a pioneer round of tra

117 e evidence that phospho-Upf1 functions after nonsense codon recognition during steps that involve the

118 Collectively, the results suggest that nonsense codon recognition may occur in the nucleus.

119 sed the effectiveness with which an upstream nonsense codon reduces mRNA abundance.

120 ation of TCR-beta transcripts in response to nonsense codons requires several features of translation

121 ed decay when inserted into exon 6 between a nonsense codon residing in exon 6 and intron 6.

122 the frameshift mutation or a closely located nonsense codon resulted in half the normal mRNA level.

123 uridine into pseudouridine (Psi), ref. 4) of nonsense codons suppresses translation termination both

124 se compounds, including PTC124, fail to show nonsense codon suppression activity when Renilla renifor

125 ntified in a cell-based FLuc assay as having nonsense codon suppression activity.

126 Using the in vivo nonsense codon suppression method for incorporating unna

127 A nonsense codon suppression technique was employed to inc

128 genetic defect, called 'exon skipping' and 'nonsense codon suppression'.

129 discovered in an FLuc-based assay targeting nonsense codon suppression, is an unusually potent FLuc-

130 tus determines viability as a consequence of nonsense codon suppression.

131 from mutation-specific treatments, including nonsense codon suppressors and exon skipping, to gene th

132 modified amino acid in response to the amber nonsense codon TAG.

133 ly and with high fidelity in response to the nonsense codon TAG.

134 of the ecotin gene containing an engineered nonsense codon (TAG) at the positions of interest.

135 For example, the amber nonsense codon, TAG, together with orthogonal Methanococ

136 serts sulfotyrosine in response to the amber nonsense codon, TAG.

137 natural amino acids in response to the amber nonsense codon, TAG.

138 A) for the tryptophan codon (TGG) causing a nonsense codon (TGA) at residue 498 within the transmemb

139 o modify this pair to decode either the opal nonsense codon, TGA, or the four-base codon, AGGA, limit

140 se codon at position 23 has been the 5'-most nonsense codon that has been analyzed.

141 on 127ins5 and epsilon 553del 7) generates a nonsense codon that predicts truncation of the epsilon s

142 lls from the deleterious effects of in-frame nonsense codons that are generated by routine inefficien

143 of GPx1 mRNA positions the boundary between nonsense codons that do and do not elicit NMD, as has be

144 function in positioning the boundary between nonsense codons that do and do not mediate decay, the ef

145 Nonsense codons that prematurely terminate translation g

146 hibition (GINI), to identify genes harboring nonsense codons that underlie human diseases.

147 ns trigger faster rates of decapping than 3' nonsense codons, thereby providing a mechanistic basis f

148 drug, PTC124, was identified that suppresses nonsense codon translation termination.

149 These results suggest that nonsense codons trigger a decay pathway distinct from th

150 We also show that 5' nonsense codons trigger faster rates of decapping than 3

151 In this pathway, a nonsense codon triggers accelerated deadenylation that p

152 Nonsense codons upstream of and including position 192 o

153 d using reversion analysis of an Ig V region nonsense codon (Vn).

154 The nonsense codon was found in a conserved haplotype in the

155 The mutant allele contains a nonsense codon which truncates the 1,317-amino-acid prot

156 apid turnover of an mRNA containing an early nonsense codon, which is degraded by a deadenylation-ind

157 tants also enhance translational fidelity at nonsense codons, which correlates with a reduction in to

158 ncipal NMD factor, Upf1p, or by flanking the nonsense codon with a normal 3'-untranslated region (UTR

159 acylated amino acid in response to an amber nonsense codon with translational fidelity greater than

160 The new orthogonal pair suppresses amber nonsense codons with an efficiency roughly comparable to

161 anine (tfm-Phe) into proteins in vivo at TAG nonsense codons with high translational efficiency and f

162 Insertion of nonsense codons within the Fed-1 coding sequence disrupt

163 ion of near-cognate tRNAs at the site of the nonsense codon without apparent effects on transcription