ライフサイエンスコーパス: notochord

1 ltimately leads to bifurcation of the caudal notochord.

2 dary tail containing both somitic muscle and notochord.

3 urchin, and the nematode and in the chordate notochord.

4 age and other cartilage-like tissues such as notochord.

5 he presumptive epidermis and surrounding the notochord.

6 floor plate and inhibits contribution to the notochord.

7 equired for ntl expression in the developing notochord.

8 contribute to either the floor plate or the notochord.

9 e kidney fusion, even in the presence of the notochord.

10 Xbp1 and Creb3l2 were also activated in the notochord.

11 ssed but not differentially activated in the notochord.

12 of the intervertebral disc develops from the notochord.

13 th retardation and incomplete closure of the notochord.

14 tive streak markers overlies the prospective notochord.

15 morphological boundary around the developing notochord.

16 ral of which are expressed in the developing notochord.

17 fically localized to the outer border of the notochord.

18 seudo-epithelium immediately adjacent to the notochord.

19 ck of yolk extension, and a kinked posterior notochord.

20 ate volumetric growth in the spinal cord and notochord.

21 diates surprisingly strong expression in the notochord.

22 ards the midline and coalesce underneath the notochord.

23 ith one key issue being the emergence of the notochord.

24 on and stiffening, gave rise to the chordate notochord.

25 t the zebrafish embryo with exception of the notochord.

26 ession of cEbf1 is regulated by Shh from the notochord.

27 cEbf1 is controlled by Shh signals from the notochord.

28 for specification and differentiation of the notochord.

29 ermal tissues in the dorsal isolate, and the notochord, a central structure involved in patterning ve

30 The notochord, a conserved axial structure required for embr

31 displays unambiguous vertebrate features: a notochord, a pair of prominent camera-type eyes, paired

32 During development of the notochord--a structure akin to the vertebrate spine--in

33 Tissue manipulations (notochord ablation) and Shh gain and loss of function ex

34 Interestingly, while notochord abnormalities are not observed following parti

35 ly regulated by a change in signaling by the notochord along the anteroposterior axis.

36 lls of the NP are thought to derive from the notochord, although adult NP lacks identifiable notochor

37 lino oligonucleotide injection caused a wavy notochord and cardiovascular abnormalities with a reduce

38 col8a1a), and cause folding of the embryonic notochord and consequently adult vertebral column malfor

39 e shaped and positioned by C&E alongside the notochord and differentiate into skeleton, fast, and slo

40 Then, sheath cells invade the inner notochord and differentiate into vacuolated cells, there

41 spinal cord levels, Shh produced by both the notochord and floor plate (FP) diffuses dorsally to orga

42 Here, we showed that disruption of the notochord and floor plate by diphtheria toxin (DTA)-medi

43 Notochord and floor plate cells are sources of molecules

44 vatives that arise in close proximity to the notochord and floor plate, it has been assumed that thei

45 ties, we specifically inactivated Shh in the notochord and floor plate.

46 centers including the dorsal marginal zone, notochord and floorplate.

47 hought to arise from transformed remnants of notochord and have a predilection for the axial skeleton

48 ion of apoptosis decreases the length of the notochord and it is severely kinked.

49 For example, the notochord and mesenchyme are induced by FGF/MAPK signali

50 eurula stages, followed by a failure to form notochord and muscle and then the partial loss of trunk

51 Removal of hedgehog signaling in the notochord and nearby floorplate resulted in the formatio

52 ortant for convergent extension in the mouse notochord and neural plate, the results indicate that ch

53 ibutes descendants to the paraxial mesoderm, notochord and neural tube, and is serially transplantabl

54 shment of asymmetric cell fates in the Ciona notochord and neural tube.

55 eted from the axial signaling centers of the notochord and prechordal plate functions as a morphogen

56 m traveling across the anterior limit of the notochord and propagating down the right LPM.

57 tes in having a dorsal, hollow nerve cord, a notochord and somites.

58 he closing neural folds and ventrally in the notochord and somites.

59 tails yielded second regenerates containing notochord and spinal cord but little or no muscle.

60 insights into the mechanisms that safeguard notochord and spine development.

61 anterior streak derivatives namely the node, notochord and the emerging definitive endoderm.

62 plate, while cell fate specification of the notochord and the floor plate, as well as signaling with

63 rom Hensen's node of the chick embryo to the notochord and the floor plate.

64 s were then performed to analyse whether the notochord and/or Shh regulate cEbf1 expression.

65 ryos with hydrocephaly and abnormally curved notochords and overall body shape, whereas published kno

66 a3/4/5 is highly expressed in the developing notochord, and Cs-lamalpha3/4/5 protein is specifically

67 the vertebrate dorsal midline (floor plate, notochord, and hypochord) has been an area of classical

68 y localized to the presumptive epidermis and notochord, and play a critical and unexpected role in po

69 es involved in locomotion, including muscle, notochord, and the central nervous system.

70 Interestingly, the notochord appears to be the only embryonic structure in

71 brafish, the "adaxial" cells adjacent to the notochord are the first muscle precursors to be specifie

72 definitive endoderm, as well as the node and notochord, arises at the same time but mostly in cells t

73 RA, which marks the early mesoderm, node and notochord, arises in Oct4 expressing cells on days 3-4.

74 We further identify the mouse notochord as a rich source of multiple redundant neurona

75 Moreover, the Ciona notochord as a single-file array of forty polarized cell

76 is likely that multiple mechanisms shape the notochord as it converges and extends.

77 mesodermal derivatives including muscle and notochord, as well as within the nervous system.

78 directed convergent extension in presumptive notochord at gastrulation.

79 The disruption of the notochord band has been observed with IVD degeneration.

80 Furthermore, Noggin mimics notochord-based inhibition by preventing mesodermal EC g

81 We find apoptotic cells in the notochord beginning at the neural groove stage and incre

82 ediolateral intercalation but forms a robust notochord boundary.

83 isense morpholinos led to strong deficits in notochord but not somitic muscle development.

84 sed expansion of hypochord at the expense of notochord, but floor plate was not affected.

85 romotes floor plate and hypochord fates over notochord, but has variable effects on Shh expression in

86 secretory pathway genes was observed in the notochord, but not in notochord precursors in the axial

87 set of axial mesoderm that gives rise to the notochord, but not prechordal mesoderm, which gives rise

88 de, an arrangement which is seen in ascidian notochords, but which has not been observed in other mod

89 Finally, we show that bends and kinks in the notochord can lead to aberrant apposition of osteoblasts

90 ic development of mesoderm-derived posterior notochord, cardiac, and hematopoietic tissues.

91 We describe new evidence for a notochord, cartilaginous arcualia, gill pouches, articul

92 During aortae fusion, the notochord ceases to exert its negative influence on vess

93 to drive medial precursor cells towards the notochord cell fate.

94 sibling cell volume asymmetries that precede notochord cell intercalation; the developmental timing o

95 ds the middle; and the differential rates of notochord cell narrowing after intercalation.

96 n of the embryonic axis, as well as abnormal notochord cell polarity.

97 s, which consist of variable dislocations of notochord cells along the anterior-posterior axis.

98 e segments containing transplanted wild-type notochord cells but not in the ones containing wild-type

99 small nuclei pulposi were formed, with most notochord cells dispersed throughout the vertebral bodie

100 As notochord cells form nuclei pulposi, we propose that the

101 Here, we perform RNAseq on flow-sorted notochord cells from multiple stages to define a compreh

102 chm notochord cells initiate intercalation normally, but the

103 chord extension through PCP-controlled CE of notochord cells, establishing a role for Wnt11 in mammal

104 ation of polarized actin-rich protrusions in notochord cells, resulting in defective notochord interc

105 rm cells and encase the inflating vacuolated notochord cells.

106 ents are unable to activate transcription in notochord cells.

107 d low-level ShhN in the prechordal plate and notochord, consistent with the notion that ShhN can rapi

108 P) position, with cells in the middle of the notochord consistently wider than cells at the anterior

109 Notochord convergence and extension, reduced but not abs

110 s, including the brain, spinal cord, retina, notochord, cranial skeleton and muscle, and can transact

111 Serial deletions of the 581-bp notochord CRM revealed that this sequence is also able t

112 requires Ci-Bra, and identified a Ci-Tbx2/3 notochord CRM that necessitates multiple Ci-Bra binding

113 and FoxA in the activation of an individual notochord CRM, and highlights the importance of transcri

114 tic characterization of Brachyury-downstream notochord CRMs.

115 ignaling is not required for floor plate vs. notochord decisions and plays a minor role in floor plat

116 can, knockdown of Fukutin or FKRP leads to a notochord defect and a perturbation of laminin expressio

117 of individual zebrafish, we correlate focal notochord defects of the embryo with vertebral malformat

118 hat the lacZ insertions interfered with some notochord-dependent aspect of vertebral development.

119 We find that cell shape in the intercalated notochord depends strongly on anterior-posterior (AP) po

120 function affects only development of caudal notochord derivatives and is compensated for in its othe

121 e marker, downstream to and regulated by the notochord derived Shh, which may be functionally involve

122 e to ECs and to form blood vessels, but that notochord derived-BMP antagonists suppress EC differenti

123 During development, embryonic notochord-derived cells (NDCs) are the direct progenitor

124 tiation of the endocardial progenitors while notochord-derived Hh is a likely source for the specific

125 rectly observe, measure, localize and modify notochord-derived Shh ligand in the context of neural pa

126 Since notochord-derived signals are essential for formation of

127 Notochord-derived Sonic Hedgehog (Shh) is essential for

128 This work shows that this notochord-derived tissue continues to carry out a major

129 Surrounding tissues, such as the notochord, dermomyotome, and sclerotome also exhibit dif

130 he T (brachyury) gene, which is important in notochord development and is expressed in most sporadic

131 dicate that apoptosis is required for normal notochord development during the formation of the anteri

132 In this study we show that early notochord development in Xenopus embryos is regulated by

133 class transcription factors is required for notochord development.

134 ession and nutrient availability critical to notochord development.

135 In vertebrates, the notochord develops by convergence and extension of the c

136 otochord precursor pool is depleted, and the notochord differentiates prematurely.

137 Notochord differentiation and morphogenesis are severely

138 nchymal PSM while blocking somitogenesis and notochord differentiation.

139 We also report that the notochord diminishes the ability of mature ECs to organi

140 The Ciona notochord displays planar cell polarity (PCP), with ante

141 is markedly sensitizes developing embryos to notochord distortion if copper availability is diminishe

142 the copper-dependent lysyl oxidases, causes notochord distortion in the zebrafish embryo identical t

143 he lysyl oxidase substrate col2a1 results in notochord distortion when combined with reduced copper a

144 n demonstrates that loss of loxl1 results in notochord distortion, and that loxl1 and loxl5b have ove

145 The notochord does not contact the TVCs but contributes to t

146 constituents, defining the structure of the notochord during aging is critical for investigations re

147 or maintaining the rod-like structure of the notochord during early embryonic development.

148 alized expression of pld1 is observed in the notochord during early segmentation, in the somites duri

149 odules that begin with the elongation of the notochord during gastrulation in the rapidly developing

150 high-throughput screen identified synthetic notochord enhancers that are activated by the combinatio

151 ode enabled in silico discovery of bona fide notochord enhancers, including those containing low-affi

152 (WGEF) gene by a microarray-based screen for notochord enriched genes, and show that WGEF is involved

153 find only modest overlap between this set of notochord-enriched transcripts and the genes upregulated

154 l be invaluable for testing hypotheses about notochord evolution.

155 This set of putative effector genes with notochord expression conserved from tunicates to vertebr

156 112 of the Ciona notochord genes have known notochord expression in vertebrates, more than twice as

157 At the same time, notochord expression is maintained after Sox9a knockdown

158 We determined that the notochord expression of Ciona Tbx2/3 (Ci-Tbx2/3) require

159 Both genes regulate trunk notochord extension through PCP-controlled CE of notocho

160 to posterior pattern that is correlated with notochord extension through vacuolization.

161 Mouse embryos that lack notochords fail to form cohesive aortic vessels because

162 posterior structures, including neural tube, notochord, fin, and muscle.

163 ls never give rise to midline tissues of the notochord, floor plate and dorsal endoderm, raising the

164 tain the axial identity of prechordal plate, notochord, floor plate and hypochord progenitors during

165 r expression in craniofacial cartilage, ear, notochord, floor plate, hypochord and fins in a pattern

166 ing induces notochord within a population of notochord/floor plate bipotential cells through negative

167 ryos and acts as a Wnt antagonist to promote notochord formation and prevent muscle differentiation.

168 erning in the dorsal organizer by inhibiting notochord formation and promoting hypochord and possibly

169 anscription factor Brachyury is required for notochord formation in all chordates, and that it contro

170 lts in absent melanin pigmentation, impaired notochord formation, and hindbrain neurodegeneration.

171 er stages in development ntl is required for notochord formation, and our analysis has also led to th

172 of the neural tube and somites by regulating notochord formation, and provide evidence that both gene

173 Blastopore closure, notochord formation, somite development, neural tube clo

174 is a characteristic and necessary feature of notochord formation.

175 t loxl1 and loxl5b have overlapping roles in notochord formation.

176 er sufficient resolution to discriminate the notochord from the surrounding the nucleus pulposus, esp

177 ate into vacuolated cells, thereby restoring notochord function and allowing normal spine development

178 se axial defects do not arise from perturbed notochord function, as cellular proliferation, apoptosis

179 es a foundation for systems-level studies of notochord gene regulation and morphogenesis.

180 are complicated by the limited knowledge of notochord genes and cis-regulatory modules (CRMs) that a

181 Orthologs of 112 of the Ciona notochord genes have known notochord expression in verte

182 The full set of Ciona notochord genes provides a foundation for systems-level

183 specifier Macho-1 and 50 Brachyury-regulated notochord genes, as well as several anti-neural factors

184 f which overlap with known Ci-Bra-downstream notochord genes.

185 The notochord gives rise to spinal segments during developme

186 During later mouse embryogenesis, the notochord gives rise to the middle part of the intervert

187 The assessment and imaging of the notochord has classically relied on histological techniq

188 and cause developmental defect in the brain, notochord, heart, and kidney, depending on the delivery

189 ent at the dorsal midline, prechordal plate, notochord, hypochord and floor plate share a common embr

190 t in the tailbud to specify hypochord from a notochord/hypochord bipotential cell population.

191 somites and neural plate mirrors that of the notochord in these embryos, and the somites are severely

192 formation of the posterior mesoderm and the notochord in vertebrate embryos.

193 emonstrate that morphological defects of the notochord in zebrafish can generate congenital-type spin

194 e the complete anteroposterior axis, but not notochord, in embryos blocked in FGF signaling.

195 The role of axial structures, especially the notochord, in metanephric kidney development has not bee

196 Sonic hedgehog (Shh) ligand secreted by the notochord induces distinct ventral cell identities in th

197 n1b, we show that caveolae are essential for notochord integrity.

198 of FGF3 in the developing nerve cord directs notochord intercalation through non-MAPK signaling.

199 s in notochord cells, resulting in defective notochord intercalation.

200 The notochord is a defining feature of the chordate body pla

201 At the center of the vertebrate notochord is a large fluid-filled organelle, the notocho

202 e of the developing neural tube, whereas the notochord is a rod of axial mesoderm that lies directly

203 The vertebrae notochord is a transient rod-like structure that produce

204 The notochord is considered an evolutionary novelty and one

205 These results suggested that the notochord is dispensable for nephrogenesis but required

206 ence that function of Pld1 in the developing notochord is essential for vascular development in verte

207 The notochord is necessary for the development of the chorda

208 limited in range, and they suggest that the notochord is necessary for the normally observed longer

209 y cascading through the entire length of the notochord is not supported; instead a more complex mecha

210 is morphological feature of having a tapered notochord is present in many chordates.

211 ehog (Shh) secreted from the floor plate and notochord is required for specification of ventral (audi

212 We have previously shown that the notochord is responsible for the generation and maintena

213 The notochord is the defining characteristic of the chordate

214 signaling) secreted from the midline by the notochord, it is unknown how fusion is later signaled.

215 l stage such that, by the tadpole stage, the notochord lacks any recognizable structure, although not

216 Our strategy was to remove the notochord, leaving the overlying notoplate intact, and d

217 Some cells misdirected from the notochord lineage were found to be incorporated into def

218 d lacks any recognizable structure, although notochord markers are expressed in a normal temporal pat

219 rachyury, indicating that Brachyury is not a notochord master regulator gene as strictly defined.

220 cellular analysis suggests that Shh from the notochord might traffic into the neural target field by

221 mediolateral cell polarity is important for notochord morphogenesis, it is likely that multiple mech

222 tropicalis, with prominent expression in the notochord, nervous system and stomach.

223 rapidly developing frogs, elongation of the notochord occurred earlier relative to the time point of

224 o assay based on misexpressing Ci-MRF in the notochord of Ciona embryos.

225 2 targeting a gene that is expressed in the notochord of early embryos and in multiple epithelia dur

226 The notochord of the ascidian Ciona consists of only 40 cell

227 The notochord of the ascidian Ciona provides a unique model

228 es in the development of a simple organ: the notochord of the ascidian Ciona savignyi.

229 sential regulator of tubulogenesis using the notochord of the invertebrate chordate Ciona intestinali

230 compared the transcriptome in the developing notochord of Xenopus laevis embryos with that of other e

231 oderm is nearly twice as stiff as either the notochord or neural plate, and at least 10-fold stiffer

232 cal basis for zebrafish and mouse bifurcated notochord phenotypes as well as the rare human congenita

233 the simple chordate Ciona intestinalis, the notochord plate consists of just 40 cells, which undergo

234 es, and review evidence in teleosts that the notochord plays an instructive role in segmental pattern

235 The notochord plays critical structural and signaling roles

236 Thus, the vertebrate notochord plays important structural roles beyond early

237 The leftward nodal flow across the posterior notochord (PNC) has been identified as the earliest even

238 f ciliated cells of the mouse COA (posterior notochord, PNC), we can restore fluid flow, asymmetric e

239 In the absence of Bmp4, the notochord precursor pool is depleted, and the notochord

240 es was observed in the notochord, but not in notochord precursors in the axial mesoderm at early gast

241 m-ectoderm border, decreased adhesion at the notochord-presomitic mesoderm border, and tension at bou

242 ost abundant BMP antagonist expressed in the notochord prior to fusion, undergoes a dramatic downregu

243 genesis, possibly by maintaining cohesion of notochord progenitors by regulation of cadherin localiza

244 s work sheds light on a large section of the notochord regulatory circuitry controlled by T-box facto

245 heir contributions to the development of the notochord remain uncharacterized.

246 Searching for an explanation, we found that notochord remnants near incipient spheno-occipital synch

247 ic domains, we describe the structure of the notochord remnants with aging in the lumbar IVDs of BALB

248 The appearance of the notochord represented a milestone in Deuterostome evolut

249 ation of polarity along the long axis of the notochord requires the PCP pathway, a role we demonstrat

250 mutant forms of Ci-Tbx2/3 in the developing notochord revealed a role for this transcription factor

251 ed and then restored, underscoring the Ciona notochord's amenability for in vivo studies of PCP.

252 lement, we analyzed the morphogenesis of the notochord sheath cells as they withdraw from the stack o

253 Here, we show that increased notochord sheath collagen deposition in polycystin 2-def

254 lls form nuclei pulposi, we propose that the notochord sheath functions as a "wrapper" around the not

255 The notochord sheath mineralizes normally, supporting the id

256 Our data suggest that the formation of the notochord sheath requires hedgehog signaling and that th

257 ate resulted in the formation of an aberrant notochord sheath that normally surrounds this structure.

258 In the absence of the notochord sheath, small nuclei pulposi were formed, with

259 required for the structural integrity of the notochord sheath.

260 , apoptosis, and expression of regulators of notochord signaling are normal in Pbx1/Pbx2 mutants.

261 The timing of notochord, somite, and neural development was analyzed i

262 l reveals a statistical enrichment of Dvl in notochord-somite boundary-(NSB)-directed protrusions, wh

263 Ciona, in which the single-copy Brachyury is notochord-specific and CRMs are easily identifiable, to

264 ctivity, we have identified a 581-bp minimal notochord-specific cis-regulatory module (CRM) whose act

265 d extends toward the midline and expresses a notochord-specific combination of genes.

266 le model for such investigations in a 155-bp notochord-specific CRM from the ascidian Ciona intestina

267 ry code" of sequence and syntax features for notochord-specific expression.

268 , leprecan was identified as a target of the notochord-specific transcription factor Ciona Brachyury

269 tes Brachyury (Ci-Bra), a key determinant of notochord specification.

270 regenerate its tail, including skin, muscle, notochord, spinal cord and neurons and blood vessels.

271 r copper in the development of the zebrafish notochord, suggesting that specific cuproenzymes are req

272 ed by a paracrine input of Tgfbeta3 from the notochord, suggesting that the former programs the hemog

273 Here we report that the notochord suppresses the generation of ECs from the meso

274 s as well as the rare human congenital split notochord syndrome.

275 We find that ascidian notochord taper involves three main mechanisms: Planar C

276 Among the Ci-Tbx2/3 notochord targets are evolutionarily conserved genes, in

277 In some tissues such as lamprey notochord, the collagen fibrillar organization is natura

278 ly in the absence of Nap1/WAVE activity: the notochord, the layers of the heart, and the epithelial-t

279 populate not only neurectoderm, somites and notochord throughout the axis, but also the chordoneural

280 diffraction data from native and derivative notochord tissue samples to solve the axial, D-periodic

281 rant bone deposition at regions of misshapen notochord tissue.

282 n of this apoptosis causes the length of the notochord to approximately double compared to controls.

283 We also reveal polarity in the notochord to be dynamic: a cell's polarity state can be

284 d sheath functions as a "wrapper" around the notochord to constrain these cells along the vertebral c

285 tal day 0, representing opposite ends of the notochord to NP transformation.

286 Midline progenitors can be transfated from notochord to somite fate after gastrulation by ectopic e

287 upregulated by ectopic expression of the key notochord transcription factor Brachyury, indicating tha

288 tiple stages to define a comprehensive Ciona notochord transcriptome.

289 chord is a large fluid-filled organelle, the notochord vacuole.

290 We establish that notochord vacuoles are required for body axis elongation

291 Here we show that zebrafish notochord vacuoles are specialized lysosome-related orga

292 The notochord was adjacent to the dorsal foregut endoderm du

293 on of the spinal cord isolated together with notochord was used, and responses to bending were record

294 as active in specific nonneural cells of the notochord when placed with APPb gene promoter proximal e

295 n that the NP cells arise from the embryonic notochord, which acts as a major signaling center in the

296 box sites that are utilized by Ci-Bra in the notochord, which are also bound in vitro by the muscle-s

297 position of prospective adaxial cells to the notochord, which is achieved by convergence movements, i

298 while allowing expression to persist in the notochord, which underlies the neural tube during neurog

299 Mechanisms coordinating notochord-wide polarity remain elusive, but appear to en

300 Wnt signaling induces notochord within a population of notochord/floor plate b