ライフサイエンスコーパス: nuclear extract

1 hylated or unmethylated probes and HeLa cell nuclear extract.

2 sites but blocked U2 snRNP assembly in HeLa nuclear extract.

3 bind and compete for HIF with hypoxic Hep3B nuclear extract.

4 RN protein, compared with BLM, from the HeLa nuclear extract.

5 ombined plasmid, repair oligonucleotide, and nuclear extract.

6 chromatin, we fractionated a mammalian cell nuclear extract.

7 n PADT, we developed a PADT assay using HeLa nuclear extract.

8 ulated by a THZ1-sensitive factor present in nuclear extract.

9 ll line nuclear extract and regressing-tumor nuclear extract.

10 nd a partially purified fraction from a HeLa nuclear extract.

11 ndonuclease 1 (APE1) within few nanograms of nuclear extract.

12 stably paused polymerases in the presence of nuclear extract.

13 ET and CYC from the female mosquito fat body nuclear extract.

14 proximal pausing to DSIF-depleted Drosophila nuclear extracts.

15 processing of histone pre-mRNA in mammalian nuclear extracts.

16 stone H2A acetylation activity in Drosophila nuclear extracts.

17 not inhibit (CAG)(n) hairpin repair in HeLa nuclear extracts.

18 nce of TFs in glaucomatous than in normal TM nuclear extracts.

19 K8beta pre-mRNAs in vitro in the presence of nuclear extracts.

20 SN)-BER, was observed with mitochondrial and nuclear extracts.

21 nd highly purified protein was prepared from nuclear extracts.

22 OA-treated or cycloheximide-treated HBC cell nuclear extracts.

23 essing by altering the sumoylation status of nuclear extracts.

24 n purify DNA methyltransferase activity from nuclear extracts.

25 tion of p53 proteins with antibody to Sp1 in nuclear extracts.

26 g by proteins of the Jun and Fos families in nuclear extracts.

27 sically associates with cohesin in HeLa-cell nuclear extracts.

28 MAL1 throughout the circadian cycle in liver nuclear extracts.

29 pendent, HJ branch migrator present in human nuclear extracts.

30 use IgM gene to support RNA splicing in HeLa nuclear extracts.

31 ic binding of a consensus probe for c-Myc to nuclear extracts.

32 ver nuclear extracts relative to adult liver nuclear extracts.

33 pair (NER) was examined using Xenopus oocyte nuclear extracts.

34 ite stable, remaining on the DNA template in nuclear extracts.

35 se tissues compared with their corresponding nuclear extracts.

36 genous p68 and p53 co-immunoprecipitate from nuclear extracts.

37 ed to perform in vitro transcription in HeLa nuclear extracts.

38 dative damages on transcription in HeLa cell nuclear extracts.

39 teins that are stably associated with WRN in nuclear extracts.

40 ty to the labeled repressor element in hPTEC nuclear extracts.

41 lity shift assays (EMSAs) with Jurkat T-cell nuclear extracts.

42 ach to identify H1-binding proteins in human nuclear extracts.

43 e removed from Pol II by a factor present in nuclear extracts.

44 and purified DNMT enzymatic activities from nuclear extracts.

45 ome DNA repair and replication proteins from nuclear extracts.

46 her strand causes RNAPII arrest in HeLa cell nuclear extracts.

47 vitro processing efficiency of highly active nuclear extracts.

48 eased histone acetyl transferase activity in nuclear extracts.

49 that co-purify with TDP-43 from rodent brain nuclear extracts.

50 ssociates with a CtBP corepressor complex in nuclear extracts.

51 n FLAP promoter as demonstrated by EMSA with nuclear extracts.

52 NA methyltransferase (DNMT) within embryonic nuclear extracts; (2) uptake of TDCIPP from 0.75 h postf

53 ift assays (EMSAs) we have now identified in nuclear extracts a functional repressor cis element, (T/

54 ubation with a reassembly mixture containing nuclear extract also encapsidated a reporter plasmid.

55 ed retardation of repair of the AP site with nuclear extract and an elevated mutation frequency after

56 enome" was dependent on the presence of both nuclear extract and ATP.

57 ssibility even when Geminin is combined with nuclear extract and chromatin in vitro.

58 cally interact with HIV-1 IN in both soluble nuclear extract and chromatin-bound fractions.

59 the EJC core component eIF4A3 from HeLa cell nuclear extract and found that eIF4A3 is dispensable for

60 tein (ACA; n = 13) were cultured with a HeLa nuclear extract and normal PBMCs.

61 increased with the concentration of retinal nuclear extract and oligonucleotide.

62 loped an in vitro assay using mammalian cell nuclear extract and plasmid DNA containing bulky adducts

63 The ENE inhibits deadenylation and decay in nuclear extract and prevents deadenylation of naked RNA

64 Both HOXA9 in EC nuclear extract and recombinant HOXA9 protein bound to t

65 n vitro polyadenylation assay with HeLa cell nuclear extract and recombinant Hu proteins, we have sho

66 footprint analysis by the walleye cell line nuclear extract and regressing-tumor nuclear extract.

67 Gel shift experiments with nuclear extracts and a CpG-methylated DNA probe indicate

68 phorylation at Thr(359)/Ser(363) in cellular/nuclear extracts and co-immunoprecipitation with cellula

69 ontribution of MHEJ to DNA end joining using nuclear extracts and DNA substrates containing 10-bp rep

70 P ubiquitin ligase activities were absent in nuclear extracts and exclusively cytoplasmic.

71 etinoid X receptor (RXR) heterodimer in HeLa nuclear extracts and identified its polypeptide componen

72 ified Mip40-containing complexes from testis nuclear extracts and identified tMAC, a new testis-speci

73 5 inhibits the splicing of mRNA in cell-free nuclear extracts and in a cell-based dual-reporter mRNA

74 -p53 as determined by immunoprecipitation of nuclear extracts and in vitro.

75 ycT1:CDK9/P-TEFb and Tat:P-TEFb complexes in nuclear extracts and interacts with recombinant Tat:P-TE

76 repair experiments employing Xenopus oocyte nuclear extracts and lesion-containing DNA substrates de

77 ed by a significant increase of NF-kappaB in nuclear extracts and phosphorylated p38 MAPK in cell lys

78 NF-kappaB in nuclear extracts and phosphorylated p38 MAPK, ERK, and J

79 Using mammalian cells, Xenopus nuclear extracts and purified proteins, we show that aft

80 proteins of the ATR-Chk1 pathway using both nuclear extracts and purified proteins.

81 ers of ATF4, we applied ROS17/2.8 osteoblast nuclear extracts and purified recombinant His-ATF4 onto

82 We purified nuclear extracts and quantified nuclear factor-kappaB (N

83 vitro, Western blot analysis of cellular and nuclear extracts and reverse transcription-PCR.

84 hat Oct4 interacts with H3K56ac in mouse ESC nuclear extracts and that perturbing H3K56 acetylation d

85 taining and PARP cleavage (immunoblotting of nuclear extracts) and cardiac function by transthoracic

86 bly reduced total HDAC activity in vitro (in nuclear extracts) and in vivo (in protoplasts).

87 using either in vitro translated proteins or nuclear extract, and by in vivo chromatin immunoprecipit

88 with recombinant VDR/RXRalpha and with JEG-3 nuclear extract, and was verified in living JEG-3 cells

89 s with U5snRNP proteins and tri-snRNP110K in nuclear extracts, and facilitates recognition of an alte

90 n an approximately 670-kD protein complex in nuclear extracts, and physically interacts with other kn

91 hatidylinositol 3-kinase (PI3-K) subunits in nuclear extracts, and that the p110 subunit of PI3-K dir

92 P sites with a vicinal 8-oxoG form DSBs with nuclear extract, as confirmed in vivo by transformation

93 In nuclear extracts, association of ER with a biotinylated

94 ds and 5' Okazaki-fragment ends or, in human nuclear extracts, at nicks in exogenous circular substra

95 By contrast, an unfractionated nuclear extract-based system in which Mediator has been

96 the activity of H3K4 demethylases in Beas-2B nuclear extracts because ambient oxygen tensions were re

97 DLX5 in MC-4 cell nuclear extracts binds to the C site in vitro.

98 noblotting to identify at least 17 CoRs from nuclear extracts bound to 17beta-estradiol (E2)-liganded

99 omoter bound NFAT proteins derived from IMCD nuclear extracts, but they selectively bound different N

100 kappaB DNA binding activity was evaluated in nuclear extracts by electrophoretic mobility shift assay

101 ts both 3'- and 5'-directed excision in HeLa nuclear extracts by only 20-30%.

102 Measurement of these proteins in nuclear extracts by SRM permitted the reproducible quant

103 iased affinity chromatography screen of HeLa nuclear extracts by using either mock-treated or ERK2-ph

104 nsis-infected host cells and was detected in nuclear extracts by Western immunoblotting with TRP120-s

105 S]) have low levels of NF-kappaB activity in nuclear extracts comparable with normal marrow, while pa

106 evels were minimal in DEX-treated macrophage nuclear extracts, compared with those from GM-CSF-treate

107 Immunolocalization and ELISA of nuclear extracts confirmed increased nuclear STAT3 in re

108 Western blotting of tissue and SCp2 nuclear extracts confirmed that CDP150 lacks the C termi

109 Nuclear extracts containing Metnase decatenated kDNA mor

110 4 and increase in phospho-Smad2 in the liver nuclear extract correlated with elevated miR-181b/d in m

111 ity shift assays (EMSA) using bovine retinal nuclear extract demonstrate that RA response elements (R

112 Gel shift assays using lens nuclear extracts demonstrated interactions of Pax6, Maf,

113 alysis of ACF immunopurified from hepatocyte nuclear extracts demonstrated phosphorylation of serine

114 evious studies of exon IIIc splicing in HeLa nuclear extracts demonstrated that this guanine branchsi

115 Purification from nuclear extract demonstrates that B23 contributes to DNA

116 e results were consistent with the fact that nuclear extracts derived from a limited number of AD pat

117 Immunodepletion of NELF or DSIF from a nuclear extract desensitizes transcription in vitro to D

118 eLa cells, we show that hCDK13 purified from nuclear extracts displays CTD kinase activity in vitro,

119 tion sequence that eliminate Elba binding in nuclear extracts disrupt the early boundary activity of

120 TAF(I)41 immunodepletion from nuclear extracts dramatically reduces Pol I transcriptio

121 Previous work showed that, in HeLa nuclear extracts, DSBs with partially complementary 3' o

122 is, addition of arachidonic acid to isolated nuclear extracts enhanced the binding of PPARdelta to DR

123 lement (ERE)-DNA pull-down assays using HeLa nuclear extracts followed by mass spectrometry-immunoblo

124 NPK and XRN2 from intact and RNase A-treated nuclear extracts followed by shotgun mass spectrometry r

125 Moreover, analysis of nuclear extracts fractionated by column chromatography f

126 Using a cell-free transcription system with nuclear extract from 3T3-L1 preadipocytes and rC/EBPbeta

127 Nuclear extract from HCT116, HepG2, or DLD1 cells bound

128 In addition, nuclear extract from human fibroblasts harboring a mutat

129 Further, nuclear extract from resected colon cancers showed eleva

130 monstrated mobility shift in the presence of nuclear extract from TCDD-treated EL4 cells.

131 We performed gel shift assays using nuclear extract from testes, brain, and hypothalamus.

132 Furthermore, nuclear extract from the SM22 transfectants showed dimin

133 t in the glucose-responsive complex of liver nuclear extract from which ChREBP was purified.

134 to radiolabeled ARE much more strongly than nuclear extracts from AA-untreated cells.

135 e-strand telomeric DNA binding activities in nuclear extracts from B. oleracea, partial purification

136 DNase protection assays using nuclear extracts from BAFF-R-expressing cells suggested

137 ctrophoretic mobility supershift assays with nuclear extracts from beta-cell lines and mouse islets,

138 Nuclear extracts from bovine articular chondrocytes trea

139 on with this sequence was demonstrated using nuclear extracts from cadmium-treated cells.

140 he Galpha(i2) gene promoter was decreased in nuclear extracts from cells pretreated with antioxidants

141 Nuclear extracts from cells treated with DHA or PA had a

142 etic mobility shift assays demonstrated that nuclear extracts from CHX-treated and CHX + TCDD cotreat

143 muscle nuclear extract was used but not when nuclear extracts from cultured myotubes were used.

144 Comparing activated p53 binding in nuclear extracts from doxorubicin- or ionizing radiation

145 A transcription factor ELISA, using nuclear extracts from EC and control muscle samples, sho

146 EMSA analysis using nuclear extracts from either A549 cells or human lung ti

147 Moreover, with nuclear extracts from ERbeta-Deltaex3 prostates, there w

148 zed and the repair investigated using either nuclear extracts from hamster cells proficient (AA8) or

149 Nuclear extracts from hDFs cultured in DBP/collagen spon

150 rocedures on both purified protein and crude nuclear extracts from HEK 293 cells.

151 ate that all CCAAT box sites bind factors in nuclear extracts from Hek293.

152 We observed that nuclear extracts from HeLa cells incorporated a signific

153 KCS protein binding activity (KBP) by using nuclear extracts from HeLa cells not treated with IFN.

154 clear GR and GR-DNA binding were measured in nuclear extracts from hippocampus, hypothalamus, prefron

155 confirmed by electromobility shift assays in nuclear extracts from human lung fibroblasts.

156 ding to the cognate sequence was enriched in nuclear extracts from hypoxic but not normoxic RAW 264.7

157 evealed induction of DNA binding activity in nuclear extracts from hypoxic RAW cells, with supershift

158 EMSA analysis using nuclear extracts from IMCD cells showed that both the -1

159 The results of RNA affinity analysis with nuclear extracts from intact liver demonstrated that the

160 transillumination of protein gels containing nuclear extracts from K3-treated cells revealed a promin

161 We show that the LBC is enriched in nuclear extracts from late, but not early, embryos and t

162 he ability to bind NF-kappaB proteins within nuclear extracts from lipopolysaccharide (LPS)-induced m

163 t can be adapted to study mismatch repair in nuclear extracts from mismatch repair proficient and def

164 Electrophoretic mobility shift assays using nuclear extracts from MLE-15 murine Clara cell-derived m

165 Ets consensus binding motif were shifted by nuclear extracts from MLE15 cells.

166 In this study, we used nuclear extracts from murine erythroleukemia cells to pu

167 Pull-down assays using nuclear extracts from osteoblasts or COS-7 cells overexp

168 NA binding complex by supershift analysis of nuclear extracts from oxysterol-treated, cultured kerati

169 trate stage-specific demethylase activity in nuclear extracts from primary chicken erythroid cells th

170 ys using both purified proteins and isolated nuclear extracts from primary human fibroblasts and 293T

171 Analysis of nuclear extracts from sheep PT tissue collected 90 min a

172 I footprint analysis on the HPV16 URR using nuclear extracts from TGF-beta-sensitive HPV16-immortali

173 lpha and C/EBP-beta, GATA-1, AP-2, PAX-6) in nuclear extracts from the remnant livers.

174 thylation at Arg3, are readily acetylated by nuclear extracts from the same cells, if and only if the

175 d that DNA occupancy of NF-kappaB present in nuclear extracts from tumour necrosis factor alpha (TNFa

176 d by Western blots comparing Dlgh1 levels in nuclear extracts from wild type and Cx32 null murine liv

177 Examination of nuclear extracts from WISH cells treated with IFN-gamma

178 SB202190-treated cell nuclear extract had about 50% increase in Oct-1 binding

179 bodies that detect O-GlcNAc in cytosolic and nuclear extracts have been described previously.

180 ofiles of ARTD1 (PARP1) and ARTD2 (PARP2) in nuclear extracts highlighted the semi-complementary, yet

181 core promoters when assayed with crude human nuclear extracts (HSK, SNF, or Dignam).

182 rinting and gel-shift assays with human lung nuclear extract identified two DNA elements bound by C/E

183 MS/MS analysis of HREC nuclear extracts identified the heterodimer of transcrip

184 tarate-dependent histone H3K9 demethylase in nuclear extract in vitro.

185 NRF-1, and SP1 motifs prevent DNA binding in nuclear extracts in both keratinocytes and liver.

186 nt tissues and species and also in HeLa cell nuclear extracts in vitro.

187 tor in complex media (e.g., 250 mug/mL crude nuclear extracts) in a convenient, low-reagent process r

188 ic mobility shift assays using mouse retinal nuclear extracts, in combination with specific antibodie

189 within large complexes present in HeLa cell nuclear extracts, in which the hyperphosphorylated form

190 ibody to PRPF40A precipitated U2 snRNPs from nuclear extracts, indicating that PRPF40A associates wit

191 h these findings, the depletion of CDK9 from nuclear extracts inhibited Tax transactivation in vitro.

192 Depletion of the SMN complex proteins from nuclear extracts inhibits formation of the E complex and

193 alyses, we observed that, in the presence of nuclear extract, intact VLPs partially disassemble, prov

194 eriments indicated that Ref-1/Ape present in nuclear extract interacted with HIF-1 and p300 but not A

195 However, the addition of HeLa nuclear extract is able to reconstitute the DNA binding

196 DNA binding activity present in A. thaliana nuclear extracts is not dependent on POT1a or POT1b prot

197 hift assay and transcription factor array in nuclear extract isolated from whole retina.

198 set of SNORDs, including SNORD27, in soluble nuclear extract made under native conditions, where fibr

199 Conversely, nuclear extracts made from cells lacking alpha-cat show

200 propriately, Drosophila dCDK12 purified from nuclear extracts manifests CTD kinase activity in vitro.

201 g the G allele of this promoter region bound nuclear extracts more avidly.

202 system, using TFIIB-immunodepleted HeLa cell nuclear extract (NE).

203 nificantly (P = 0.002) elevated in the liver nuclear extract of mice fed CDAA diet.

204 ncoded transcription factor E47 are lower in nuclear extracts of activated splenic B cells from old m

205 Moreover, the nuclear extracts of AD cells contained proteins that pro

206 gh NF-kappaB activity increased in the liver nuclear extracts of both genotypes after DEN exposure, t

207 RFX1 binding complexes were found in the nuclear extracts of C6 cells.

208 etic mobility shift assays demonstrated that nuclear extracts of cells overexpressing E2F1 bound dire

209 Although pS776-ATXN1 is enriched in nuclear extracts of cerebellar cells, the vast majority

210 d stage-specific demethylase activity in the nuclear extracts of chicken red cells; activity in 5-, 8

211 We now show that nuclear extracts of HeLa cells contain LSD1 that is asso

212 From nuclear extracts of high light-treated Arabidopsis plant

213 -kDa silencer-binding protein (SBP) from the nuclear extracts of human breast cells BT-549 and MDA-MB

214 ing of these molecules by mismatch repair in nuclear extracts of human cells.

215 ha-endonuclease-dependent mismatch repair in nuclear extracts of human cells.

216 Nuclear extracts of isolated rat islets cultured with 0.

217 demonstrated increased NFAT-DNA binding from nuclear extracts of lipopolysaccharide (LPS) stimulated

218 ated that Sp1 was present in cytoplasmic and nuclear extracts of neonatal myocardium.

219 man insulin from standard solutions and from nuclear extracts of pancreatic cells with high selectivi

220 dicate that all elements bind factors in the nuclear extracts of PC-3-M cells.

221 NF-4 serum caused a supershift not only with nuclear extracts of scN-adapted insect midgut but with t

222 found after pol iota was immunodepleted from nuclear extracts of the cells.

223 The CAGGTG motif binds E47 in nuclear extracts of the mutating cells.

224 DNase I footprinting with nuclear extracts of transaldolase-expressing cell lines

225 at the NF-kappaB subunit p50 predominated in nuclear extracts of whole cecal tissue and of isolated c

226 In addition, the complex formed by nuclear extract on this probe contained Elk-1, as shown

227 we reported that fractionation of HeLa cell nuclear extracts on glycerol gradients revealed an endog

228 Depletion of B23 from nuclear extracts or knockdown B23 in PC12 cells abolishe

229 Depletion of Ebp1 from nuclear extracts or knockdown of Ebp1 in PC12 cells abol

230 not inhibit histone deacetylase activity in nuclear extracts or reactivate the expression of the den

231 repair system and that depletion of RFX from nuclear extracts or RFX knockdown in cells reduces misma

232 gnition site, unless it is preincubated with nuclear extracts precleared by anti-Pax-6 but not by ant

233 region of the WDSV long terminal repeat with nuclear extract prepared from a walleye cell line reveal

234 n and some of these factors were enriched in nuclear extracts prepared from the brain.

235 etic mobility shift assay (EMSA) analysis of nuclear extracts prepared from ultraviolet B (UVB)- and

236 ERT promoter DNA-protein complexes formed in nuclear extracts prepared only from lung cancer cells bu

237 Moreover, alkaline phosphatase treatment of nuclear extracts reduced the amount of APOBEC-1 co-immun

238 assays was low in embryonic day (E) 19 liver nuclear extracts relative to adult liver nuclear extract

239 Immunodepletion of FLNA from nuclear extracts resulted in a decrease in rDNA promoter

240 wn spiked and endogenous LSD1 from HeLa cell nuclear extracts, setting the stage for activity-based d

241 In vitro analyses using nuclear extracts show that at the downstream site, Hu pr

242 plasts and Western blotting and MALDI-TOF of nuclear extracts show that Avp triggers Avpr1alpha local

243 Tax-containing nuclear extracts showed increased DNA-PK activity, and s

244 Gel shift assays using transfected myoblast nuclear extracts showed that ectopic p107 reduced Sp1 oc

245 This complex was isolated from HeLa cell nuclear extracts stably expressing LANA and was verified

246 ain homogenates and separate cytoplasmic and nuclear extracts suggesting nuclear ErbB-back-signaling

247 chondrial extract, but not with cytosolic or nuclear extract, suggesting that cleavage of APE1 by a s

248 o-immunoprecipitated from the bovine retinal nuclear extract, suggesting their existence in a multi-p

249 ing HO-1 immunoreactive band was enriched in nuclear extracts, suggesting that HO-1 was cleaved to al

250 ous PIAS1 and TBP co-immunoprecipitated from nuclear extracts, suggesting the interaction occurred in

251 d lack of DNA binding activity in macrophage nuclear extracts suggests that the NF-kappaB site is non

252 Using the Xenopus nuclear extract system, here we show that the Dna2 nucle

253 tivity is required for elongation in a crude nuclear extract system, whereas in a purified system dev

254 ltered binding of regulatory proteins in all nuclear extracts tested, and -1002 T > G resulted in low

255 A kinase in HeLa nuclear extract that caused the shift was partially puri

256 ator is essential for basal transcription in nuclear extracts that contain a more physiological compl

257 inds a protein present in neuronal precursor nuclear extracts that express very low levels of reelin

258 or HNE-dG adducts did not occur in XPA cell nuclear extracts that lack the capacity for NER.

259 icroscopy and by immunodetection in purified nuclear extracts that the ankyrin repeat-containing prot

260 an in vitro transcription system, using HeLa nuclear extract, that supports not only efficient splici

261 Therefore, in Xenopus nuclear extracts, the rotational orientation of CTDs on

262 asal transcription and pol II recruitment in nuclear extract, thus indicating a conditional restricti

263 tein deacetylase enzymes present in the HeLa nuclear extracts, thus making it potentially suitable no

264 d as bait for factors present in BCBL-1 cell nuclear extract to show that PAN RNA interacts with seve

265 in hypoxic pulmonary artery endothelial cell nuclear extract to the WT sequence failed to alter seque

266 s-like particles (VLPs), were incubated with nuclear extracts to provide access to the range of cellu

267 ibody in the Western blotting of mouse liver nuclear extracts to show that PXR is accumulated in the

268 , L, and A2/B1 from both HeLa and hepatocyte nuclear extracts to the element further supports its act

269 recipitation and rate-zonal sedimentation of nuclear extracts together with double immunostaining of

270 In yeast nuclear extracts, transcription induces stable TAF bindi

271 were measured in whole cell, cytoplasmic, or nuclear extracts, using colorimetric assays.

272 Protein was captured from fibroblast nuclear extracts, using oligonucleotides representing IR

273 When nuclear extract was fractionated on glycerol gradients,

274 A proteolyzed nuclear extract was separated on the methyl-affinity col

275 ing complex when using adult skeletal muscle nuclear extract was used but not when nuclear extracts f

276 ity in 5-, 8-, and 11-day-old erythroid cell nuclear extracts was 6, 76, and 24%, respectively.

277 ncreasing the concentration of actin in HeLa nuclear extracts was able to suppress overall HDAC funct

278 Using in vitro transcription with nuclear extract, we demonstrate that THZ1, a covalent Cd

279 Using a hypothalamic nuclear extract, we showed that the -248 T allele result

280 measurements of DNA-protein interactions in nuclear extracts, we elucidate the dual functions of PER

281 ng PI(3,4,5)P(3) column and NGF-treated PC12 nuclear extracts, we identified B23 as a nuclear PI(3,4,

282 Using fractionated nuclear extracts, we identified the transcription factor

283 ll-free apoptotic assay and NGF-treated PC12 nuclear extracts, we purified Ebp1 as a factor, which co

284 old) changes in the ratio of DNA template to nuclear extract were sufficient to change Pol II-mediate

285 zed by Western blot for HO-1, and HSP-70 and nuclear extracts were analyzed by DNA mobility shift ass

286 Nuclear extracts were depleted of U1 snRNP with a concom

287 in binding to DNA in diabetic versus control nuclear extracts were observed with electrophoretic mobi

288 ectrophoretic mobility shift assays with 3T3 nuclear extracts were used to study the PR1C core promot

289 element-binding protein regulatory motifs in nuclear extracts when mice reach adulthood.

290 cantly, phosphorylated ACF was restricted to nuclear extracts where it co-sedimented with 27S editing

291 urther confirmed by Western blot analysis of nuclear extracts wherein levels of RelA, the p65 compone

292 s were readily detected in the infected cell nuclear extracts which were significantly reduced by ERK

293 lso presented for generating cytoplasmic and nuclear extracts, which extends use of this protocol to

294 ays show that Pin1 inhibits transcription in nuclear extract, while an inactive Pin1 mutant in fact s

295 Accordingly, we treated Hep3B nuclear extract with a C/EBP-binding consensus oligonucl

296 Immunoprecipitation of HeLa nuclear extract with anti-galectin-3 resulted in the cop

297 Furthermore, treatment of HTD114 nuclear extracts with an antibody against DNA ligase I o

298 ch inhibits ATR immunoprecipitated from HeLa nuclear extracts with an IC50 of 5 nM and ATR mediated p

299 CTCF and Suz12 are coprecipitated from nuclear extracts with antibodies specific for either pro

300 Electromobility shift assays (EMSA) of nuclear extracts with oligomers from the promoting regio