ライフサイエンスコーパス: nuclease

1 t degradation, which is extended by the EXO1 nuclease.

2 at has rarely been observed with any type of nuclease.

3 y, cell type and duration of exposure to the nuclease.

4 forks, including being processed by the Exo1 nuclease.

5 dsDNA degradation by a trans-acting helicase-nuclease.

6 e essential function and is resistant to the nuclease.

7 pe I-E CRISPR-Cas (Cascade) complex and Cas3 nuclease.

8 ethylsulphate, potassium permanganate and S1 nuclease.

9 ucleic acids using CRISPR RNA (crRNA)-guided nucleases.

10 t concentrations and degradation mediated by nucleases.

11 have invigorated the field of site-specific nucleases.

12 e required for catalysis in analogy to other nucleases.

13 crRNA-guided DNA cleavage by the Cpf1 family nucleases.

14 al sequence that are completely resistant to nucleases.

15 n with transcription activator-like effector nucleases.

16 0-fold protection against digestion by serum nucleases.

17 ngs to the Rad2 family of structure-specific nucleases.

18 ys that protect the ends of some ncRNAs from nucleases.

19 BLM) helicases, or the GEN-1 or MRT-1 (SNM1) nucleases.

20 Although FANCD2-associated nuclease 1 (FAN1) contributes to ICL repair, FAN1 mutati

21 Herein, we quantitatively explore the nuclease accessibility of the nucleic acid as a function

22 ciated score that quantifies tissue-specific nuclease accessibility significantly improves prediction

23 lizes to the break, protecting DNA ends from nuclease accessibility, and recruits additional NHEJ fac

24 in understanding the role and regulation of nucleases acting at stalled forks with a focus on the nu

25 ntitative description of protein binding and nuclease activation at off-target DNA sequences remains

26 y the balance between target recognition and nuclease activation for precision genome editing.

27 ion sets up the catalytic selectivity of the nuclease active site for genome stability.

28 However, the R2H2.CglI complex has only one nuclease active site sufficient to cut one DNA strand su

29 ree the 3'-end of aa-tRNA for entry into the nuclease active site.

30 which allows us to model DNA binding in the nuclease active site.

31 ic and coordinated actions of MRE11 and CtIP nuclease activities are required to limit the stable loa

32 ed, suggesting roles for both Mre11 and Sae2 nuclease activities in promoting the processing of DNA e

33 tegration was independent of the helicase or nuclease activities of Cas3.

34 dard tumor suppressor and instead imply that nuclease activities of MRE11 are required for oncogenesi

35 meiotic breaks requires both Mre11 and Sae2 nuclease activities.

36 re11 is required for DNA end recognition and nuclease activities.

37 onal enzyme that possesses both helicase and nuclease activities.

38 g MRN, or harboring MRN in which MRE11 lacks nuclease activities.

39 in cell differentiation, tRNA modification, nuclease activity and protein dephosphorylation.

40 ts of activity at those sites when assessing nuclease activity and specificity.

41 e show that the Cas1 subunits repress Cas2/3 nuclease activity and that foreign DNA recognition by th

42 assays, we find that stimulation of Artemis nuclease activity by X4-LIV and the efficiency of blunt-

43 Our study indicates that targeting TSN nuclease activity could inhibit pathological cell prolif

44 RISPR) targeting method that restores FnCas9 nuclease activity in a target-specific manner.

45 ow that 5'-exonuclease is the most prevalent nuclease activity in endo-lysosomal compartments and tha

46 of CysK:CdiA-CT dissociation, ensures robust nuclease activity in target bacteria.

47 ues, which are sufficient to direct specific nuclease activity in vitro and in vivo with reduced off-

48 ith micromolar affinity, but did not display nuclease activity in vivo.

49 Inhibition of MRE11 nuclease activity increased DNA damage and selectively i

50 In contrast, Sae2 nuclease activity is essential for DNA repair when the M

51 15 kb from the DSB that was dependent on the nuclease activity of Dna2-Sgs1.

52 ative resection initiation pathway where the nuclease activity of MRN apparently directly cleaves the

53 Tetramer assembly activates the cos-cleavage nuclease activity of the enzyme, which matures the genom

54 to inhibit HSV replication also inhibit the nuclease activity of UL12.

55 er the growth defect was a result of loss of nuclease activity or another function of UL12, we introd

56 tion-of-function mutants that lack intrinsic nuclease activity or the ability to promote Mre11 endonu

57 al intracellular environment for caspase and nuclease activity, key components of programmed cell dea

58 morphological effect of the 3D track on the nuclease activity, which suggested that the performance

59 ry site for DNA binding, and is required for nuclease activity.

60 g in different degrees of protection against nuclease activity.

61 ral DNA into constituent units utilizing its nuclease activity.

62 ffinity and this interaction is critical for nuclease activity.

63 urvival, particularly in the absence of Dna2 nuclease activity.

64 development of specific inhibitors of MUS81 nuclease activity.

65 dently from its functions in promoting Mre11 nuclease activity.

66 y opposing roles in the regulation of Cas2/3 nuclease activity.

67 the degradation of nascent DNA by the MRE11 nuclease after replication stress.

68 Similarly, both S1 nuclease and 2D gel electrophoresis of DNA topoisomers d

69 ss of the effector, the fitness costs of the nuclease and effector, and the completeness of parasite

70 Here, using in vitro nuclease and ligation assays, we find that stimulation o

71 talytic core of MRN/X comprised of the Mre11 nuclease and Rad50 adenosine triphosphatase (ATPase) act

72 ecome 'swapped out' in the dimer, increasing nuclease and ribose binding activities by 100-fold and 1

73 tants of two target proteins (staphylococcal nuclease and ribose binding protein).

74 es of pathways involving structure-selective nucleases and alternative complexes, which can act upon

75 plicable methods for delivering programmable nucleases and donor templates for homology-directed repa

76 Transcription factors, various polymerases, nucleases and histones recognize and bind DNA with diffe

77 combined efficient expression of engineered nucleases and integration-defective lentiviral vector (I

78 oes not share sequence similarity with these nucleases and lacks the characteristic disulfide bonds o

79 acteristics, including resistance to various nucleases and proteases.

80 Furthermore, replicons carrying CRISPR/Cas9 nucleases and repair templates achieved GT at an endogen

81 we demonstrate the use of multiple designer nucleases and variant-aware library design to interrogat

82 A aptamers are susceptible to degradation by nucleases, and for this reason, RNA-based sensors are sc

83 1/BRCA1), the MUS-81, EXO-1, SLX-1 and FAN-1 nucleases, and the DOG-1 (FANCJ) helicase in ICL resolut

84 have been pursued to increase resistance to nucleases, and while it seems likely that these and othe

85 These RNA-guided nucleases are powerful weapons in the fight against fore

86 The nuclease ARTEMIS is essential for the development of B a

87 or cellular V(D)J recombination and in vitro nuclease assays with C-terminally truncated ARTEMIS show

88 Methylation Specific Nuclease-assisted Minor-allele Enrichment (MS-NaME) empl

89 The RNA-guided Cpf1 (also known as Cas12a) nuclease associates with a CRISPR RNA (crRNA) and cleave

90 dden Markov Models for the identification of nuclease bacteriocins (NBs) in bacteria of which, to-dat

91 elopment within the vector, and then use the nuclease-based homing reaction as a form of gene drive t

92 get genome modification, than a current Cas9 nuclease-based method, and can install disease-correctin

93 To develop a CRISPR nuclease-based platform that would enable higher efficie

94 repeat (CRISPR)/CRISPR-associated protein 9 nuclease (Cas9) system depends on a guide RNA (gRNA) to

95 dromic repeats (CRISPR)-associated protein-9 nuclease (Cas9), a method called acoustic-transfection.

96 A paralogous operon involves a putative nuclease (CinB) rather than a DUB.

97 logy-directed repair (HDR) coupled with Cas9 nuclease cleavage has been used with great success to re

98 that, if not repaired quickly, are prone to nuclease cleavage, resulting in DSBs.

99 The RNA-guided Cpf1 nuclease cleaves double-stranded DNA targets complementa

100 emonstrate a significant association between nuclease colicins, NBs specific for Escherichia coli, an

101 The HerA-NurA helicase-nuclease complex cooperates with Mre11 and Rad50 to coor

102 re the bacterial orthologs of Mre11-Rad50, a nuclease complex essential for genome stability, normal

103 pendent formation and activation of this tri-nuclease complex provides a unique mechanism by which ce

104 es identified depended on sgRNA sequence and nuclease concentration.

105 at prometaphase by formation of the SMX tri-nuclease containing three DNA repair structure-selective

106 palindromic repeats and the Cas9 RNA-guided nuclease (CRISPR/Cas9) system provides a new opportunity

107 ther CRISPR interference (CRISPRi) or CRISPR nuclease (CRISPRn).

108 ibe Cleavage Under Targets and Release Using Nuclease (CUT&RUN), a chromatin profiling strategy in wh

109 Here, we utilize nuclease-deactivated Cas9 protein fused to repetitive pe

110 The nuclease-deactivated variant of CRISPR-Cas9 proteins (dC

111 Complete nuclease-dead AdnAB enzyme can sustain recombination in

112 nding partners for measuring the kinetics of nuclease-dead Cas9 (dCas9) interactions.

113 ng the known custom DNA-binding modules, the nuclease-dead Streptococcus pyogenes Cas9 (dCas9) protei

114 The generation of nuclease deficient versions of Cas9 has enabled the deve

115 s-splicing to ligate synthetic elements to a nuclease-deficient Cas9 (dCas9) in vitro and subsequentl

116 Using an in vivo biotinylated nuclease-deficient Cas9 protein and sequence-specific gu

117 viral replication through the analysis of a nuclease-deficient viral mutant.

118 Furthermore, using DNase I in a nuclease degradation assay, G4-T-oligo was found to be m

119 lecules sheltered in the spiky layer against nuclease degradation, exhibiting no significant transfec

120 eutic efficacy by reducing susceptibility to nuclease degradation.

121 epair via homology-based mechanisms involves nuclease-dependent DNA end resection, which generates lo

122 IP is absolutely required for activating the nuclease-dependent mechanism of Mre11 but not the nuclea

123 ; 3) preparation of chromatin by micrococcal nuclease digest; 4) ChIP for open chromatin-associated h

124 c polymers that are both highly resistant to nuclease digestion and capable of cross-pairing with DNA

125 to demonstrate that dsRNA is protected from nuclease digestion by virus-induced membrane invaginatio

126 Furthermore, restriction nuclease digestion revealed a strongly reduced accessibi

127 h a backbone structure that is refractory to nuclease digestion, makes TNA an attractive biopolymer s

128 r extract system, here we show that the Dna2 nuclease directly initiates the resection of clean DSBs

129 n NHEJ, followed by the iterative binding of nucleases, DNA polymerases, and the XRCC4-DNA ligase IV

130 n this study, we replaced this domain with a nuclease domain from Salmonella enterica subsp. arizonae

131 The nuclease domain is dispensable for DNA binding but resid

132 wn about the catalytic state of the Cas9 HNH nuclease domain, and identifying how the divalent metal

133 translocation conformationally restrict the nuclease domain, inhibiting cleavage; TerL release from

134 We report the structure of the P74-26 TerL nuclease domain, which allows us to model DNA binding in

135 orax (SET) histone methylase and transposase nuclease domain.

136 +/- 9.2 min-1 upon removal of the C-terminal nuclease domain.

137 an N-terminal ATPase domain and a C-terminal nuclease domain.

138 cleavage suggests flexible tethering of the nuclease domains during DNA cleavage.

139 sid upon completion of packaging unlocks the nuclease domains to cleave DNA.

140 osine triphosphatase (ATPase) and C-terminal nuclease domains.

141 e, we show that endonuclease G, an apoptotic nuclease downstream of Caspase-3, is directly responsibl

142 S-NaME) employs a double-strand-specific DNA nuclease (DSN) to remove excess DNA with normal methylat

143 presence of an enzyme called duplex specific nuclease (DSN), however, a fraction of the surface-bound

144 edox mediators, p19 protein, duplex specific nucleases (DSN) and redox cycling.

145 ase is more active than the three individual nucleases, efficiently cleaving replication forks and re

146 Gene editing with engineered nucleases enables site-specific genetic modification of

147 m T-DNA, biolistics or by stably integrating nuclease-encoding cassettes and repair templates into th

148 suppressed by inactivation of the resection nuclease Exo1.

149 repair; the enzyme belongs to a nonspecific nuclease family that includes the apoptotic endonuclease

150 ctions in Drosophila cells using Micrococcal Nuclease followed by sequencing.

151 Cas2, with the type I effector helicase and nuclease for invader destruction, Cas3.

152 bind foreign DNA and recruit a trans-acting nuclease for target degradation.

153 X, SLX4 co-ordinates the SLX1 and MUS81-EME1 nucleases for Holliday junction resolution, in a reactio

154 The RNA-guided CRISPR-Cas9 nuclease from Streptococcus pyogenes (SpCas9) has been w

155 eloped by measuring the activity of secreted nuclease from the bacteria via a modified DNA oligonucle

156 Crystal structures of the large terminase nuclease from the thermophilic bacteriophage G20c show t

157 sertion or replacement, we screened the Cpf1 nucleases from Francisella novicida and Lachnospiraceae

158 xonuclease activity and shares homology with nucleases from other members of the Herpesviridae family

159 novicida possesses novel properties, but its nuclease function is frequently inhibited at many genomi

160 find that the ability of Sae2 to promote MRX nuclease functions is important for DNA damage survival,

161 d drug-inducible catalytically inactive Cpf1 nuclease fused to transcriptional activation domains to

162 (CRISPR)-CRISPR-Associated Protein 9 (Cas9) nuclease gene editing is potentially an important tool f

163 ng the transcription activator-like effector nuclease gene editing technology, we have knocked out bo

164 HspB1-null cells, generated by CRISPR/Cas9 nuclease genome editing, display an abrogated stretch-st

165 cids in a sequence-specific manner using Cas nucleases guided by short RNAs (crRNAs).

166 The Cas9 nuclease has been adapted to target epigenomic modificat

167 Genome editing using programmable nucleases has revolutionized biomedical research.

168 However, these nucleases have also been shown to cut at off-target site

169 Designer nucleases have gained widespread attention for their abi

170 ion process, by crRNA-displaying Cascade and nuclease-helicase fusion enzyme Cas3, respectively.

171 tes complementary targets, and Cas3 executor nuclease/helicase.

172 element of the KRAS gene contains a GC-rich nuclease hypersensitive site with three potential DNA se

173 ses to cold tend to contain more micrococcal nuclease hypersensitive sites in their promoters, a prox

174 The KRAS nuclease-hypersensitive element (NHE) region contains a

175 le, the human KRAS proto-oncogene contains a nuclease-hypersensitive element located upstream of the

176 bind foreign DNA and recruit a trans-acting nuclease (i.e., Cas2/3) for target degradation.

177 AdnAB, the helicase-nuclease implicated in mycobacterial HR, consists of two

178 ency of insertions and deletions elicited by nucleases in cells, tissues or embryos through analysis

179 ll molecule RNA E-AB sensors from endogenous nucleases in complex media.

180 nt and are stabilized against degradation by nucleases in human serum.

181 e for cytoplasmic translocation of RNase III nucleases in response to virus in diverse eukaryotes inc

182 c disruption of the HIV-1 coreceptor CCR5 by nucleases in T cells is under 2 clinical trials and lead

183 guides that specify the cleavage site of Cas nucleases in the genome of the invader.

184 ward maturation and explore roles of non-Cas nucleases in this process.

185 must catch up with the preceding processing nucleases, in order to close the single-stranded gap and

186 Both nucleases, in the presence of a guide RNA and repairing

187 sferase Dnmt3a-Dnmt3L construct fused to the nuclease-inactivated dCas9 programmable targeting domain

188 RPA and was found in a complex with several nucleases, including the 5' dsDNA exonuclease EXO1.

189 Here, we report a nuclease-independent involvement of TREX1 in preventing

190 ase-dependent mechanism of Mre11 but not the nuclease-independent mechanism.

191 8%, is higher than most other genome editing nucleases, indicative of its effective enzymatic chemist

192 of genome function, but can be confounded by nuclease-induced toxicity at both on- and off-target sit

193 ucleases that may pave the way for designing nuclease inhibitors for biochemical and biomedical appli

194 cts is important for translating CRISPR-Cas9 nucleases into human therapeutics.

195 y is essential for DNA repair when the Mre11 nuclease is compromised.

196 Here we show that SMX tri-nuclease is more active than the three individual nuclea

197 forks are extensively degraded by the MRE11 nuclease, leading to chemotherapeutic sensitivity.

198 Second, FACS enrichment of cells expressing nucleases linked to fluorescent proteins can be used to

199 trand break (DSB) by a multisubunit helicase-nuclease machine (e.g. RecBCD, AddAB or AdnAB) generates

200 Yet, it is unclear how the 5'nuclease mechanisms of DNA distortion and protein orderi

201 t palindromic repeats (CRISPR) directed Cas9 nuclease-mediated gene editing of CXCR7 revealed that pr

202 ial DNA (mtDNA) damage and after zinc finger nuclease-mediated gene mutation correction, mtDNA damage

203 e RNAs that enable small molecule-controlled nuclease-mediated genome editing and small molecule-cont

204 n induced pluripotent stem cells (iPSCs) and nuclease-mediated genome editing represent a unique oppo

205 n immunoprecipitation (ChIP) and micrococcal nuclease (MNase) digest assays were performed to examine

206 y of nucleosomes, as measured by micrococcal nuclease (MNase) digestion and ATAC-seq (assay for trans

207 We performed a time-course of micrococcal nuclease (MNase) digestion and measured the relative sen

208 Micrococcal nuclease (MNase) is commonly used to map nucleosomes gen

209 view, we compare the traditional micrococcal nuclease (MNase)-based approach with a chemical cleavage

210 temporally optimized delivery of zinc finger nuclease mRNA via electroporation and adeno-associated v

211 target sequence in the genome, and the Cas9 nuclease of the system acts as a pair of scissors to cle

212 Both human and yeast Exo1 are processive nucleases on their own.

213 strategies involve digesting chromatin with nucleases or chemical cleavage followed by high-throughp

214 Albeit not excluding the agency of a backup nuclease, our findings suggest that mycobacterial HR can

215 APE2 (apurinic/apyrimidinic endonuclease 2) nuclease participates in 3'-5' nucleolytic resection of

216 Here, we show that the nuclease, polymerase, and phosphatase activities of yeas

217 se 1 (FEN1) and related structure-specific 5'nucleases precisely identify and incise aberrant DNA str

218 Inactivation of these nucleases prevents completion from occurring, and under

219 Finally, unlike nuclease-proficient Cas9 in human cells, the specificity

220 NX" DNAs <5 kb were previously isolated from nuclease-protected cytoplasmic particles in rodent neuro

221 Altered patterns in nuclease-protected small RNA fragments in emb2654 show t

222 ntification of subnucleosomal fragments from nuclease protection data represents a general strategy f

223 in the presence of competing polyanions and nuclease protection in serum relative to conventional br

224 s thus illustrate the potential for unifying nuclease protein delivery with AAV donor vectors for hom

225 These results add to our understanding of nuclease protein targets and potentially serve as starti

226 Conversely, the 3'-flap nuclease Rad1-Rad10 and enzymes known to disrupt recombi

227 editing that facilitates the testing of new nuclease reagents and the generation of edited cell pool

228 -targeted controlled cleavage by micrococcal nuclease releases specific protein-DNA complexes into th

229 Like RuvC proteins, the nuclease requires either Mn2+, Mg2+ or Co2+ ions for act

230 e (2'-F U) analogues with the aim to improve nuclease resistance and potency of therapeutic siRNAs by

231 ts can optimize properties far beyond simple nuclease resistance and that SFM4-3 should prove valuabl

232 Both 4'-OMe epimers conferred increased nuclease resistance, which can be explained by the close

233 existence of a pseudoknot that stabilizes a nuclease-resistant RNA structure in the 3' untranslated

234 g with transcription activator-like effector nucleases results in a major embryonic hemostatic defect

235 nickases (Cas9n) and dimeric RNA-guided FokI nucleases (RFNs).

236 Cas9-based RNA-guided nuclease (RGN) has emerged to be a versatile method for

237 up to 6 h, enabling full sensor function in nuclease-rich environments (undiluted serum) without the

238 ether, our results support roles for non-Cas nuclease(s) during crRNA maturation and establish a link

239 nucleotide increments, and the activity of a nuclease(s) that remains unknown.

240 Consequently, we developed a nuclease screening platform which could distinguish acti

241 Here, we show that SMALL RNA DEGRADING NUCLEASES (SDNs) initiate miRNA degradation by acting on

242 fragments are generated from much longer S1-nuclease sensitive fragments of foreign DNA that require

243 nt methods to deliver both sequence-specific nucleases (SSNs) and repair templates to plant cells.

244 provides embedded DNA strands with enhanced nuclease stability and improved cell uptake.

245 brush-DNA conjugates as a function of their nuclease stability.

246 E preferentially co-localized with the MRE11 nuclease subunit of the MRN complex and orchestrates its

247 Re-engineered protein-only nucleases such as mtZFN and mitoTALEN function effective

248 geted genetic engineering using programmable nucleases such as transcription activator-like effector

249 Programmable nucleases, such as Cas9, are used for precise genome edi

250 member of the RAD2/XPG structure-specific 5'-nuclease superfamily.

251 through Srgap2 knockout via the CRISPR/Cas9 nuclease system and conditional overexpression in the mu

252 Here we will review this RNA-guided nuclease system for gene editing with respect to its use

253 The CRISPR-Cas9 nuclease system holds enormous potential for therapeutic

254 paced short palindromic repeats (CRISPR)-Cas nuclease system is a powerful tool for genome editing, a

255 he past few years the development of several nuclease systems has broadened the range of model/cell s

256 sed on transcription activator-like effector nucleases (TALENs) and the clustered regularly interspac

257 uch as transcription activator-like effector nucleases (TALENs) is a valuable tool for precise, site-

258 ystem, transcription activator-like effector nucleases (TALENs) or zinc-finger nucleases (ZFNs).

259 In this work we use TALE nucleases (TALENs) to target a reporter construct to the

260 uorophore-labeled PCR amplicons covering the nuclease target site by capillary electrophoresis in a s

261 An mCherry-tagged nuclease targets the submicron locus, causing DNA cleava

262 novel Bace1(-/-) rat line using zinc-finger nuclease technology and compared Bace1(-/-) mice and rat

263 N1) is a multifunctional, structure-specific nuclease that has a critical role in maintaining human g

264 Trypanosomes lacking TbSNM1, a nuclease that specifically repairs ICLs, are hypersensit

265 Relaxases are metal-dependent nucleases that break and join DNA for the initiation and

266 However, we detected cellular nucleases that co-purify with Cas10-Csm, and show that C

267 ells to PARPi, indicating redundancy between nucleases that initiate HR can drive PARPi resistance.

268 ucleases (LHEs) are a class of rare-cleaving nucleases that possess several unique attributes for gen

269 ng of polymerases (Pol mu and Pol lambda), a nuclease (the Artemis.DNA-PKcs complex), and a ligase (X

270 Based on sensitivity to digestion with nucleases, the associated DNA is not in a standard doubl

271 ed random mutagenesis of the RNA-guided Cas9 nuclease to look for variants that provide enhanced immu

272 s target complementarity and governs the HNH nuclease to regulate overall catalytic competence.

273 o model an alternative strategy of using the nuclease to target an essential gene, and then linking t

274 mplying a specific role in translocating the nuclease to the cytoplasm.

275 systems utilize sequence-specific RNA-guided nucleases to defend against bacteriophage infection.

276 In addition, we used Zinc finger nucleases to generate isogenic SHANK3 knockout human emb

277 We used zinc finger nucleases to induce stable expression of human imaging r

278 inflammatory mRNAs and recruits complexes of nucleases to promote rapid mRNA turnover.

279 iption into short RNA guides that direct Cas nucleases to the invading DNA molecules.

280 RNA-interference constructs, and CRISPR/Cas9 nucleases) together with randomized, well-replicated exp

281 s through the coordinated action of multiple nucleases, topoisomerases, and helicases.

282 oughput evaluation of target specificity and nuclease toxicity in Cas9 screens.

283 Here we develop a zinc-finger nuclease translocation reporter and screen for factors t

284 ation is stimulated by eIF4E availability in nuclease-treated cell-free extracts.

285 uses, our results showed that filtration and nuclease treatment did not discernibly increase the sequ

286 A critical issue in ribosome profiling is nuclease treatment of ribosome-mRNA complexes, as it is

287 Furthermore, the same nuclease treatment proved to be useful for sensitive and

288 y (TumiD) as a cellular pathway in which the nuclease TSN promotes the decay of miRNAs that contain C

289 cribed Tudor-staphylococcal/micrococcal-like nuclease (TSN)-mediated miRNA decay (TumiD) as a cellula

290 e herpes simplex virus (HSV) type I alkaline nuclease, UL12, has 5'-to-3' exonuclease activity and sh

291 equency of targeted insertion for these Cpf1 nucleases, up to 8%, is higher than most other genome ed

292 The Rad1/XPF and Rad2/XPG nucleases were also important in protecting against cont

293 also extremely susceptible to degradation by nucleases, which are ubiquitous in the GI tract.

294 Artemis is a vertebrate nuclease with both endo- and exonuclease activities that

295 utagenesis libraries with single or multiple nucleases with incorporation of variants.

296 eplication fork stability by inhibiting DNA2 nuclease/WRN helicase-mediated degradation of stalled fo

297 restingly, knockdown of the RNA surveillance nuclease, Xrn1, and members of the CCR4-Not deadenylase

298 inally achieved thanks to these customizable nucleases; yet the rates remain to be further improved.

299 ncluding wild-type CC-125) using zinc-finger nucleases (ZFNs), genetically encoded CRISPR/associated

300 e effector nucleases (TALENs) or zinc-finger nucleases (ZFNs).