ライフサイエンスコーパス: nucleic acid

1 erminant in discriminating self versus viral nucleic acid.

2 o those of other protein complexes that bind nucleic acid.

3 lated to the starting amount of the template nucleic acid.

4 only in the presence of the specific target nucleic acid.

5 ocesses of biomolecules such as proteins and nucleic acids.

6 cellular and intracellular space for foreign nucleic acids.

7 e systems that target and deactivate foreign nucleic acids.

8 n biological systems by the encapsulation of nucleic acids.

9 s in biophysics, biochemistry and biology of nucleic acids.

10 omposed of specialized lipids, proteins, and nucleic acids.

11 which is followed by a selective cleavage of nucleic acids.

12 cusing primarily on intracellular sensors of nucleic acids.

13 g advantage of gaps between the RNAP and the nucleic acids.

14 mics of guanine amino groups in G-quadruplex nucleic acids.

15 of carbohydrates and oxidation of lipids and nucleic acids.

16 ide a platform for the cytosolic delivery of nucleic acids.

17 te immune system that sense pathogen-derived nucleic acids.

18 urface of fibrils, comparable to polymerized nucleic acids.

19 h as polyphosphate (polyP) and extracellular nucleic acids.

20 oteins, vaccines, monoclonal antibodies, and nucleic acids.

21 as developed based on a pyrrolidinyl peptide nucleic acid (acpcPNA)/polypyrrole (PPy)/silver nanofoam

22 The nucleic acid alignment of the mutated sequences against

23 nt nucleobases that can be incorporated into nucleic acids alongside their natural counterparts have

24 dimensionality and sensitive detection using nucleic acid amplification and analysis techniques.

25 Direct molecular assay (ST Direct) relies on nucleic acid amplification and solid array-based amplico

26 broad range of assays, including isothermal nucleic acid amplification techniques, enzyme-based immu

27 cluding both antigen detection and multiplex nucleic acid amplification techniques, is becoming more

28 sitive for stx by an alternative FDA-cleared nucleic acid amplification test (NAAT) but were negative

29 ple, sample-to-answer, on-demand, multiplex, nucleic acid amplification test for syndromic diagnosis

30 Here we report the development of a mobile nucleic acid amplification testing (mobiNAAT) platform u

31 rator for improving the harmonization of BKV nucleic acid amplification testing (NAAT) and enabling c

32 Nucleic acid amplification testing (NAAT) is the preferr

33 However, nucleic acid amplification testing (NAAT) with the Xpert

34 chomatis infection in certain populations by nucleic acid amplification testing (NAAT), as they invol

35 the introduction of diagnostic M. genitalium nucleic acid amplification testing including antimicrobi

36 Nucleic acid amplification testing is a very powerful me

37 other month were tested for M. genitalium by nucleic acid amplification testing.

38 DTs), digital immunoassays (DIAs), and rapid nucleic acid amplification tests (NAATs) in children and

39 a CT (ACT) (Hologic Inc., San Diego, CA) are nucleic acid amplification tests (NAATs) that detect Chl

40 The specificity of nucleic acid amplification tests (NAATs) used for early

41 At that time, nucleic acid amplification tests (NAATs) were just becom

42 retrospectively using enzyme immunoassay and nucleic acid amplification tests on stored specimens.

43 Nucleic acid amplification tests such as PCR have signif

44 ey components necessary to expand the use of nucleic acid amplification-based detection assays toward

45 ng aptamer labels, hybridization assays, and nucleic acid amplification.

46 ion of glycol nucleic acid (GNA), an acyclic nucleic acid analogue, as a modification of siRNA duplex

47 advancement here has been the translation of nucleic acid analysis to a paper-based format.

48 hemistries that will facilitate fast, simple nucleic acids analysis in a clinical setting are needed.

49 on sorter device to separate a wide range of nucleic acid analytes into distinct microchannel outlets

50 es various biosensors designed for detecting nucleic acid and protein-based cancer biomarkers for can

51 made in developing microfluidic systems for nucleic acid and whole bacteria immunoassay tests, their

52 nherent chemical differences between charged nucleic acids and hydrophobic drugs have hindered entrap

53 Recently, extracellular nucleic acids and inorganic polyphosphate (polyP) have b

54 alone is capable of binding single-stranded nucleic acids and is important for deamination.

55 ransfusion medicine but also for delivery of nucleic acids and other molecules to HSPCs for targeted

56 emergence of functional interactions between nucleic acids and polypeptides was a key transition in t

57 Interactions between nucleic acids and proteins are critical for many cellula

58 While exosomal nucleic acids and proteins have previously been explored

59 d vesicles with lipid bilayers encapsulating nucleic acids and proteins, both with and without glycos

60 tection of any fluorescent target, including nucleic acids and proteins.

61 The high charge density of nucleic acids and resulting ion atmosphere profoundly in

62 odel the electrostatic potential surrounding nucleic acids and the effects of the surrounding ion atm

63 tential and energetics to be measured within nucleic acids and their complexes with proteins.

64 valent complexes drawn from a broad class of nucleic acids and transient protein complexes found in a

65 served that in vivo administration of locked nucleic acid anti-miR-181b retarded both the development

66 Treatment with locked nucleic acid anti-miR-29a significantly improved surviva

67 primary FLT3-ITD(+) AML samples using locked nucleic acid antisense inhibitors, results in an elevate

68 rates the most differentially functionalized nucleic acid aptamer discovered by in vitro selection an

69 The combination of nanopore sensing and nucleic acid aptamer recognition comes close to this ide

70 nt lateral flow assay (DRELFA) which pairs a nucleic acid aptamer with an antibody for use as a point

71 Being nucleic acids, aptamers can be synthesized chemically or

72 perature, and the extinction coefficients of nucleic acids are also affected by temperature, which ma

73 enzymes that metabolize or modify endogenous nucleic acids are essential for preventing inappropriate

74 Nucleic acids are potent triggers for innate immunity.

75 Nucleic acids are strongly negatively charged, and thus

76 nines and no significant contribution of the nucleic acid backbone.

77 e formulated nanoparticles to carry specific nucleic acid barcodes, administered the pool of particle

78 or a covalently connected linear sequence of nucleic acid base pairs.

79 c acid based pharmaceutical development, and nucleic acid based biosensor device design.

80 biophysical insights into nuclear crowding, nucleic acid based pharmaceutical development, and nucle

81 ading to point-of-care kits that incorporate nucleic acid-based assays, including polymerase chain re

82 Functional nucleic acid-based biosensors are emerging tools that ar

83 Other nucleic acid-based methods were expensive and required t

84 Expression of miRNA was determined by locked nucleic acid-based quantitative real-time polymerase cha

85 ce reader, an important requirement toward a nucleic-acid-based point-of-care diagnostic system.

86 Control of protein levels by nucleic-acid-based technologies has proven to be a usefu

87 duces the cross predictions among the native nucleic acid binders.

88 protein, E3, which contains an N-terminal Z-nucleic acid binding (Zalpha) domain that is critical fo

89 Several variants of a nucleic acid binding motif (RRM1) of putative transcript

90 Furthermore, nucleic acid binding mutants destabilize the association

91 ne, which reveals a novel positively charged nucleic acid binding site distal to the active center th

92 The K-homology (KH) domain is a nucleic acid-binding domain present in many proteins but

93 -inducible tumor-associated protein, harbors nucleic acid-binding domains for left-handed helix (Z-fo

94 with a femtosecond-pulsed laser to bleach a nucleic acid-binding dye causing dose-dependent apoptosi

95 ese changes also affected the degradation of nucleic acid-binding protein substrates of Lon, intracel

96 The nucleic-acid-binding cleft of RNAP samples distinct conf

97 tion (RCA) is used to detect nucleic and non-nucleic acid biomarkers with high sensitivity.

98 scribed are completely generalizable to many nucleic acid biomarkers, and could be adapted to provide

99 tion between transition metal nanosheets and nucleic acids, biosensing systems can be easily assemble

100 A novel electrochemical disposable nucleic acid biosensor for simple, rapid, and specific d

101 system for the intradermal administration of nucleic acids, both plasmid DNA (pDNA) and siRNA, to tre

102 l PCR estimate the concentration of a target nucleic acid by digitizing the end-point fluorescence of

103 ion of self or foreign molecules, especially nucleic acids, by innate sensors.

104 is of the impact of deletions on proteins or nucleic acids can reveal important functional regions an

105 g systems (e.g., polypeptides, proteins, and nucleic acids) cannot occur without enrichment of chemic

106 and a new molecule (polymer, small molecule, nucleic acid, carbohydrate, etc.).

107 The unique role assumes unique protein and nucleic acid cargo.

108 We then provide effective nucleic acid combinations employed to obtain more compre

109 Here we provide a thorough analysis of nucleic acid complexes containing either halogenated bui

110 tified 21 X-bonds within known structures of nucleic acid complexes.

111 c allosteric riboswitches and small molecule-nucleic acid complexes.

112 cognized within covalently tethered HIV-1 RT-nucleic acid complexes.

113 peptides, full-length proteins, and protein-nucleic acid complexes.

114 mobile structural elements in mtRNAP and the nucleic acid components of the elongation complex (EC).

115 Integrating protein and nucleic-acid components to form engineered nucleoprotein

116 kes it difficult to accurately determine the nucleic acid concentration.

117 ection of viruses with relatively low plasma nucleic acid concentrations, it may have broad potential

118 eted exosomes carrying lipids, proteins, and nucleic acids conduct cell-cell communications within th

119 nd virus detection and analysis, and probing nucleic acid conformations and binding interactions.

120 :1 charge balance across the protein and the nucleic acid constituents, and can thus be maximal at di

121 nteractions between the involved protein and nucleic acid constituents, as well as net changes in ent

122 ample/target combinations with low levels of nucleic acids (Cq >/= 29) and/or variable amounts of che

123 afficking have been shown to strongly affect nucleic acid delivery efficiency.

124 mote the necessary conformational change for nucleic acid delivery to Pol alpha and subsequent DNA sy

125 gning and synthesizing new nanomaterials for nucleic acid delivery.

126 to examine how the molecular conductance of nucleic acids depends on the composition of their backbo

127 Rapid, inexpensive, and sensitive nucleic acid detection may aid point-of-care pathogen de

128 While highly sensitive nucleic acid detection methods and testing of multiple s

129 Microarrays and other surface-based nucleic acid detection schemes rely on the hybridization

130 ed AuNPs, with broader applications in other nucleic acid detection technologies.

131 atures (i.e., brackish vs. freshwaters), and nucleic acids (DNA vs. RNA), suggesting niche differenti

132 uration, prevents renaturation of the duplex nucleic acids (dsDNA/RNA).

133 Acridine orange (AO), a nucleic acid dye with unique spectral properties, enable

134 to measure the energetic consequences of the nucleic acid electrostatic field.

135 However, DNase treatment after nucleic acid extraction to remove host DNA significantly

136 lification reactor that combines solid-phase nucleic acid extraction, concentration, and purification

137 les for all 21 agents within 2.5 h following nucleic acid extraction.

138 opment of metal ion sensors using functional nucleic acids (FNAs) and nanomaterials.

139 cm, yet it efficiently recovers proteins and nucleic acids from a variety of pathogenic bacteria and

140 However, when analyzing nucleic acids from complex matrixes such as soil and blo

141 haea and bacteria to defend against invasive nucleic acids from phages and plasmids.

142 acid isolation membrane, the membrane binds nucleic acids from the sample.

143 Sequencing of viral nucleic acids from the stool of vaccinated, asymptomatic

144 this review, we summarize recent advances of nucleic acid-functionalized transition metal nanosheets

145 tion modifications that regulate protein and nucleic acid functions.

146 es in early RNA world evolution by enhancing nucleic acid functions.

147 and an assay of complementary gamma-peptide nucleic acid (gamma-PNA) probes conjugated to polystyren

148 Here we report the investigation of glycol nucleic acid (GNA), an acyclic nucleic acid analogue, as

149 is the extremely low capture efficiency for nucleic acids (>10 bases), which severely lowers the sen

150 New specificities of nucleic acid handling that underlie the catalytic cycle

151 Separation of nucleic acids has long served as a central goal of analy

152 enzyme-free, template-directed synthesis of nucleic acids have been limited by 'strand inhibition',

153 ion sequencing of circulating, tumor-derived nucleic acids hold promise for addressing the challenge

154 Nucleic acid hybridization-based lateral flow assay (LFA

155 a detailed understanding of the kinetics of nucleic acid hybridization.

156 Viral nucleic acid in plasma by NGMS and quantitative polymera

157 es oligonucleotide probes to enrich specific nucleic acids in heterogeneous extracts and can therefor

158 The low abundance of VZV nucleic acids in human neurons has hindered an understan

159 ination of the relative proportion of duplex nucleic acids in mixed ds/ss nucleic acid solutions, dem

160 In particular, the emergence of functional nucleic acids in the 1980s, especially aptamers, has sub

161 In this study evaluating nucleic acids in the AH from Rb eyes undergoing salvage

162 (TREX1) is an anti-viral enzyme that cleaves nucleic acids in the cytosol, preventing accumulation an

163 stranded structures formed by certain G-rich nucleic acids in vitro, but the sequence and structural

164 se (DraRnl) seals 3-OH/5-PO4 nicks in duplex nucleic acids in which the 3-OH nick terminus consists o

165 our data unveil the APE2 Zf-GRF domain as a nucleic acid interaction module in the regulation of a k

166 reveal the structural relationships between nucleic acid interactions and catalytic activity of A3F.

167 used to simultaneously detect multiple viral nucleic acid intermediates, characterize the effects of

168 livery of biomacromolecules (e.g., proteins, nucleic acids) into cell cytosol remains a critical chal

169 protects archaea and bacteria by eliminating nucleic acid invaders in a crRNA-guided manner.

170 This article reviews the state of nucleic acid ion counting measurements and critically an

171 Detection of foreign nucleic acids is an important strategy for innate immune

172 sult, chirality, a key intrinsic property of nucleic acids, is often overlooked as a design element f

173 A nucleic acid isolation membrane is placed at the reactor

174 th chaotropic agents is filtered through the nucleic acid isolation membrane, the membrane binds nucl

175 The nucleic acid lateral flow assay was based on the use of

176 The accurate calculation of protein/nucleic acid-ligand interactions or condensed phase prop

177 However, the predictive power of nucleic acid-ligand scoring functions is still a challen

178 inhibition of the let-7 family with a locked nucleic acid (LNA)-anti-miR has the opposite effect.

179 structure, assembly and function of protein-nucleic acid macromolecular machines requires multidimen

180 uses (dengue, mumps, and measles viruses) or nucleic acid material (Nipah and chikungunya viruses).

181 ules into phase-separated protein or protein/nucleic acid "membraneless organelles" that regulate a h

182 n Type III-A CRISPR-Cas immunity and central nucleic acid metabolism.

183 H structural fold defines a large family of nucleic acid metabolizing enzymes that catalyze phosphor

184 lecular structures, including complexes with nucleic acids, micelles, vesicles, hybrid nanoparticles,

185 sensitive platform used to quantify specific nucleic acid molecules amplified by polymerase chain rea

186 e formulations have been designed to deliver nucleic acids, most nanoparticles have been tested in ce

187 Previously, synthetic nucleic acid motors (3-5) and modified natural protein m

188 We have analysed nucleic acid (NA) binding and processing by full-length

189 soluble proteins (PN), polysaccharides (PS), nucleic acids (NA) and humic-like substances (HS) in the

190 This work provides access to a new set of nucleic acid-nanoparticle conjugates, which may be usefu

191 ntroduce a new concept that utilizes cognate nucleic acid nanoparticles which are fully complementary

192 s have revealed the potential in integrating nucleic acid nanostructures in cells and in vivo where t

193 tegy for constructing complex and replicable nucleic acid nanostructures, and expands the design spac

194 Simultaneously, the advance of nucleic acid nanotechnology, a platform within which rea

195 ological processes involve the stretching of nucleic acids (NAs).

196 he molecular conductance differences between nucleic acids of different backbones correlate with diff

197 In situ hybridization analysis, using locked nucleic acid oligo probes complementary to the n1-src mi

198 seful for site-specific production of dA* in nucleic acid oligomers and/or polymers and also for the

199 fferences in the conformational ensembles of nucleic acids, on the order of 1-2 A.

200 tivation, and this whether they are bound to nucleic acids or not.

201 However, mechanistic details regarding how nucleic acids or polyP modulate the individual reactions

202 dentification of crucial sequence regions in nucleic acids or proteins.

203 al synthesis and the selection of functional nucleic acids, our strategy extends these principles to

204 reactive intermediates that are important in nucleic acid oxidation.

205 n the strand replacement of dsDNA by peptide nucleic acid (PNA) and the in situ growth of electroacti

206 g with gene knockdown, we employed a peptide-nucleic acid (PNA) hybridization assay.

207 meric repeat complementing (CCCTAA)3 peptide nucleic acid (PNA) probe coupled with cardiac-specific a

208 In the current study, we describe a peptide nucleic acids (PNA)-based approach to block the ability

209 , molecularly-imprinted polymers and Peptide nucleic acid (PNAs) were developed as an attractive rece

210 nal group dependence during the evolution of nucleic acid polymer activity.

211 el active-site configuration among different nucleic acid polymerase families, (b) the origin and phy

212 f water, proteins, lipids, carbohydrates and nucleic acids present in a cell, and is tightly linked t

213 n of citrate AuNPs decorated with a specific nucleic acid probe.

214 Meanwhile, nucleic acid probes based on Watson-Crick base-pairing r

215 ic acid sensors based on fluorogenic peptide nucleic acid probes embedded in permeable, physically cr

216 cleotide-templated reactions between peptide nucleic acid probes embedded within permeable agarose an

217 tions of this model, we assay the ability of nucleic acid processing enzymes, including a DNA polymer

218 a pharmacophore modelling and information of nucleic acid properties into our graph-based signatures,

219 Natural antibodies capable of cleaving nucleic acid, protein, and polysaccharide substrates hav

220 entification of interacting residues between nucleic acid-protein complexes.

221 ication and characterization of cross-linked nucleic acid-protein heteroconjugates within a complex s

222 ristics of exosomes, their associated cargo (nucleic acids, proteins, and lipids), and downstream fun

223 ng at specific locations within G-quadruplex nucleic acids, providing valuable probes for local struc

224 Digital PCR, a state-of-the-art nucleic acid quantification technique, works by spreadin

225 progress of the HPO project since the debut Nucleic Acids Research database article in 2014, includi

226 e, previously described in the 2012 and 2014 Nucleic Acids Research Database Issues.

227 microwell, allowing for analysis of precious nucleic acid samples in shorter amounts of time relative

228 or rapid fractionation of size-heterogeneous nucleic acid samples into specific and narrow size distr

229 ecule-mediated stabilization of G-quadruplex nucleic acid secondary structures triggers local epigene

230 eport a hybridization-based assay exploiting nucleic acid self-assembly circuitry and enzyme exonucle

231 at germ cells express negative regulators of nucleic acid sensing (NAS) in steady state and applied a

232 Cytosolic nucleic acid sensing elicits interferon production for p

233 e show that this methodology can be used for nucleic acid sensing extending the analyte recognition b

234 d that NLRP14 interacted physically with the nucleic acid sensing pathway and targeted TBK1 (TANK bin

235 The discovery points to a mechanism of nucleic acid sensing regulation that may be of particula

236 mmation is independent of type I IFN and the nucleic acid sensing TLRs, blocking these pathways rescu

237 s rare human diseases caused by dysregulated nucleic acid sensing, focusing primarily on intracellula

238 covalently transform aerolysin into a highly nucleic acids-sensitive nanopore.

239 The nucleic acid sensor RIG-I is necessary to sense foreign

240 Optical nucleic acid sensors based on fluorogenic peptide nuclei

241 ut were negative for stx1 and stx2 following nucleic acid sequence analysis.

242 The amplification of the target nucleic acid sequence represents a key step for the deve

243 n clinical utility, new methods of detecting nucleic acid sequences are being developed in order to r

244 omic next generation sequencing, we detected nucleic acid sequences from 2 novel viruses in the famil

245 The isolation of specific nucleic acid sequences is a major bottleneck in molecula

246 ds that can rapidly and specifically analyze nucleic acid sequences will revolutionize the diagnosis

247 computing is to design biomolecules, such as nucleic acid sequences, that can be used to perform comp

248 correlation analysis to assess covariance of nucleic acid sequencing datasets such as chromatin immun

249 nologies such as single-molecule sensing and nucleic-acid sequencing.

250 The dense nucleic acid shell of the resulting cross-linked micella

251 A one-pot synthesis of micellar spherical nucleic acid (SNA) nanostructures using Pluronic F127 as

252 er that can autonomously move on a spherical nucleic acid (SNA)-based 3D track.

253 We have developed spherical nucleic acids (SNAs) as a blood-brain barrier-/blood-tum

254 rtion of duplex nucleic acids in mixed ds/ss nucleic acid solutions, demonstrating significant advant

255 Single-stranded nucleic acids (ssNAs) are ubiquitous in many key cellula

256 le polyelectrolytes, such as single-stranded nucleic acids (ssNAs), is complicated by the interplay o

257 chromicity of the oligonucleotide or complex nucleic acid structure.

258 DNA:RNA hybrids, nucleic acid structures with diverse physiological funct

259 erse transcriptase of the telomerase and its nucleic acid substrates leading to loss of telomerase ac

260 tructural approaches to report AID-preferred nucleic acid substrates, illuminating AID targeting mech

261 iminished by substitution of DNA for shorter nucleic acids such as mRNA or siRNA.

262 primase with the unique ability to initiate nucleic acid synthesis.

263 ystems are efficient and easily programmable nucleic acid-targeting tools, with uses reaching beyond

264 obust approach for detection of short-length nucleic acid targets, such as miRNAs.

265 od donors in resource limited settings where nucleic acid testing is not practical or feasible.

266 , and achieved 10-fold signal enhancement in nucleic acid testing of HBV as compared to the unmodifie

267 We design sequences of nucleic acids that are "guaranteed" to have long folding

268 ect can be rapidly reversed by complementary nucleic acids that break the aptamers' secondary structu

269 Although apoptotic cells (ACs) contain nucleic acids that can be recognized by Toll-like recept

270 rmation in the form of proteins, lipids, and nucleic acids that can reprogram recipient cells.

271 Aptamers are short nucleic acids that interact with a variety of targets wi

272 Nucleic acid therapeutics are limited by inefficient del

273 on the future of combinational therapy using nucleic acid therapeutics, articulating the main challen

274 employed in combination cancer therapy using nucleic acids therapeutics for successful clinical trans

275 candidate features computed from a number of nucleic acid, thermodynamic and secondary structure mode

276 Purified SUV39H1 directly binds nucleic acids through its chromodomain; and in cells, SU

277 Threose nucleic acid (TNA) and other polyelectrolytes are also c

278 alpha-l-Threofuranosyl nucleic acid (TNA) is an artificial genetic polymer comp

279 ance of both the unstructured and structured nucleic acids to accurately measure their hypochromicity

280 imultaneous delivery of drug and therapeutic nucleic acids to promote axonal regeneration and plastic

281 Cross-linking of nucleic acids to proteins in combination with mass spect

282 ially extended the recognition capability of nucleic acids to various targets, ranging from small org

283 ies can reveal atomic details of protein and nucleic acid topology and interactions between specific

284 carry donor origin-proteins, cytokines, and nucleic acids, transport these cargos to adjacent or dis

285 In this study we have used Unlocked Nucleic Acids (UNAs) to discriminate a breast cancer onc

286 Nucleic acids undergo naturally occurring chemical modif

287 l method which rapidly amplifies and detects nucleic acids using a simple device in near real-time.

288 ys protect prokaryotic cells against foreign nucleic acids using CRISPR RNA (crRNA)-guided nucleases.

289 rtant to its procoagulant activity, and that nucleic acids versus polyP may differentially modulate s

290 When terminally attached to double-stranded nucleic acids via the 5' phosphate group these fluoropho

291 o extracellular matrix proteins, lipids, and nucleic acids were tracked over pregnancy and found to b

292 Nucleic acids, which constitute the genetic material of

293 owed to be a reliable carrier for delivering nucleic acids with cytoplasmic activity such as the mRNA

294 achieving widespread delivery of therapeutic nucleic acids within brain tumors and provide a promisin

295 ding our ability to agnostically interrogate nucleic acids within diverse sample types, but in the cl

296 ponses to exogenous yet non-pathogen-derived nucleic acids would have negative consequences.

297 an evolution experiments carried out on xeno-nucleic acid (XNA) polymers require engineered polymeras

298 opy genetic information between DNA and xeno-nucleic acids (XNA) hold tremendous value as reagents in

299 Nucleic acid yield and quality metrics were evaluated in

300 Conclusion With regard to optimizing nucleic acid yields in CT-guided lung core needle biopsi