ライフサイエンスコーパス: nucleolytic

1 that DNA-PKcs does not protect DNA from the nucleolytic action of WRN.

2 ome integrity tightly controlled by specific nucleolytic activities and central homologous recombinat

3 likely involved in optimized recruitment of nucleolytic activities for the processing and protection

4 a result of this binding, Brca1 inhibits the nucleolytic activities of the Mre11/Rad50/Nbs1 complex,

5 ty and is believed to be responsible for the nucleolytic activities of the RecBCD enzyme.

6 nhibit RecBCD degradation by turning off its nucleolytic activities.

7 sn145 and Glu164, which are crucial for Spd1 nucleolytic activity and shown the active site constella

8 residues in Gre responsible for induction of nucleolytic activity in RNAP.

9 e demonstrate the existence of EndA-mediated nucleolytic activity independent of the competence state

10 indings, we posit a model whereby functional nucleolytic activity is not the feature of Rat1 that ult

11 MUS81 nucleolytic activity is required to activate compensator

12 Cyclophilin nucleolytic activity is stimulated by Ca2+ and/or Mg2+,

13 particles, we have clearly demonstrated that nucleolytic activity is the antiviral mechanism.

14 The nucleolytic activity of animal Argonaute proteins is dee

15 Transcription elongation factor GreA induces nucleolytic activity of bacterial RNA polymerase (RNAP).

16 a member of the RecQ family, stimulates the nucleolytic activity of human exonuclease 1 (hExo1), a 5

17 The nucleolytic activity of MUS81 endonuclease is required f

18 We have now evaluated the nucleolytic activity of recombinant cyclophilins under n

19 nt work, we characterize the RNA-binding and nucleolytic activity of recombinant mouse Ago2.

20 on factors GreA and GreB stimulate intrinsic nucleolytic activity of RNA polymerase (RNAP).

21 The nucleolytic activity of SLX1-SLX4 is negatively regulate

22 ed fusion protein, appeared to have very low nucleolytic activity on single-stranded (ss) DNA, which

23 capsid-associated form of AN which exhibits nucleolytic activity suggests that it may play some role

24 rvations suggest that the exosome may be the nucleolytic activity that degrades the body of the mRNA

25 ly, RPA is also required both to direct Dna2 nucleolytic activity to the 5'-terminated strand of the

26 unit of the MRN complex and orchestrates its nucleolytic activity to the ATR kinase signaling.

27 NTD is responsible for the GreA induction of nucleolytic activity while the CTD determines the bindin

28 stalled replication forks from uncontrolled nucleolytic activity, which otherwise causes irreparable

29 n of TraI that retains the sequence-specific nucleolytic activity.

30 sensitive known substrate for detecting ribo-nucleolytic activity.

31 bute substantially to competence-independent nucleolytic activity.

32 The CTD does not display any nucleolytic activity; however, it substantially increase

33 in which Ku protects chromosome termini from nucleolytic and recombinational activities but is not in

34 ty that Ku protects chromosomal termini from nucleolytic attack and functions as part of a telomeric

35 and U1.2) become reproducibly protected from nucleolytic attack by radicals.

36 shield the natural ends of chromosomes from nucleolytic attack, recognition as double-strand breaks,

37 1 and Ten1, it protects chromosome ends from nucleolytic attack, thus contributing to genome integrit

38 g, which corresponded to a trypsin-sensitive nucleolytic band of the same size.

39 y bends its DNA substrate using a bipartite, nucleolytic center formed at an N-terminal dimerization

40 DNA duplex that tracks through the enzyme's nucleolytic center.

41 DNA ends followed by 3'-->5' nicking by two nucleolytic centres within a single nuclease molecule th

42 avage by tRNA and protection of tRNA against nucleolytic cleavage by MTF.

43 in a specific DNA sequence protects DNA from nucleolytic cleavage by restriction enzymes recognizing

44 y RET1-catalyzed uridylylation, may direct a nucleolytic cleavage of multicistronic precursors.

45 plication-associated DNA damage arising from nucleolytic cleavage of stalled replication forks.

46 he two MUS81 complexes may promote different nucleolytic cleavage reactions in vivo.

47 almost all eukaryotic RNAs are generated by nucleolytic cleavage.

48 ge helix, which acts as a pivot, facilitates nucleolytic cleavage.

49 mode compared to the product of the initial nucleolytic cleavage.

50 e site, are accessible and can be removed by nucleolytic cleavage.

51 In yeast, resection is carried out by three nucleolytic complexes: Mre11-Rad50-Xrs2, which functions

52 This protein protects ssDNA from nucleolytic damage, prevents hairpin formation and block

53 Following deadenylation, nucleolytic decay of the 3'-UTR occurs generating 3' dec

54 Since the DSB ends undergo nucleolytic degradation (resection) of their 5'-ending s

55 s with BRCA2 and RAD51 to protect forks from nucleolytic degradation and (ii) it recruits the BLM hel

56 oteins protect the chromosomal terminus from nucleolytic degradation and end-to-end fusion, and they

57 ty in genetic crosses but loss of detectable nucleolytic degradation as judged by the growth of mutan

58 he BLM helicase, while protecting forks from nucleolytic degradation by MRE11.

59 Here we report that BRCA proteins prevent nucleolytic degradation by protecting replication forks

60 atural or artificial chromosomal ends, or by nucleolytic degradation from induced breaks, or nicks.

61 Chi-containing strand at Chi or switching of nucleolytic degradation from the Chi-containing strand t

62 ctopic sites of microhomology and implicated nucleolytic degradation in illegitimate DSB repair in T4

63 J when Ku70/80 is absent and are attacked by nucleolytic degradation in the absence of 53BP1.

64 HR requires nucleolytic degradation of 5' DNA ends to generate tract

65 if product is made due to template-dependent nucleolytic degradation of an internally quenched probe

66 regulator of genome integrity that restrains nucleolytic degradation of damaged replication forks.

67 Nucleolytic degradation of DNA by Exo1 is inhibited by t

68 We conclude that RecBCD enzyme's nucleolytic degradation of DNA is not necessary for intr

69 talled forks results in severe FAN1-mediated nucleolytic degradation of nascent DNA strands.

70 acting at stalled forks with a focus on the nucleolytic degradation of nascent DNA, a process common

71 mbination relies on an initial MRN-dependent nucleolytic degradation of one strand at DNA ends.

72 g to robust checkpoint signalling and slower nucleolytic degradation of the 5' strand.

73 aks by homologous recombination commences by nucleolytic degradation of the 5'-terminated strand of t

74 by the addition of cycloheximide, slows the nucleolytic degradation of the OLE1 mRNA and blocks the

75 rotects the 5' terminal viral sequences from nucleolytic degradation or from inducing innate immune r

76 of double-strand breaks (DSBs) undergo 5'-3' nucleolytic degradation to generate single-stranded DNA,

77 cleotides that exhibit partial resistance to nucleolytic degradation was dependent on both oligonucle

78 d within a substrate DNA strand impedes Exo1 nucleolytic degradation, and a 5'-terminal abasic residu

79 (ss) DNA on both strands that are exposed to nucleolytic degradation, potentially compromising genome

80 se (TERT/EST2) can protect telomeres against nucleolytic degradation.

81 RCA2 protects stalled replication forks from nucleolytic degradation.

82 RAD51 protect these structures from extended nucleolytic degradation.

83 structure, which protects the products from nucleolytic degradation.

84 hat the nucleic acids are stabilized against nucleolytic degradation.

85 horten due to incomplete DNA replication and nucleolytic degradation.

86 fail to protect their RNA/DNA contents from nucleolytic destruction.

87 However, they typically suffer from nucleolytic digestion and fast clearance from the bloods

88 t the Mre11 nuclease complex may mediate the nucleolytic digestion of the 5' strand at secondary stru

89 e benefits from both strand displacement and nucleolytic digestion, thus providing guidance for the d

90 nulus of NurA, leading to duplex melting and nucleolytic digestion.

91 are coding joints and ends demonstrates that nucleolytic end processing is dramatically reduced in jo

92 for a direct interaction of BLM with a human nucleolytic enzyme.

93 de novo synthesis and secretion of a set of nucleolytic enzymes for scavenging phosphate from extrac

94 activities of phosphate starvation-inducible nucleolytic enzymes, including ribonuclease, phosphodies

95 at RNA decay can be triggered by a prior non-nucleolytic event that marks transcripts for rapid turno

96 thin the coding region of an mRNA can effect nucleolytic events that occur at both the 5'- and 3'-end

97 revent mismatch incorporation or promote its nucleolytic excision.

98 namically regulated and how tRNAs (and their nucleolytic fragments) are centrally involved in stress

99 ription factors, by inhibiting miR171-guided nucleolytic function.

100 repair of deaminated base damage by making a nucleolytic incision one nucleotide away from the 3' sid

101 ree critical DNA repair processes, including nucleolytic incision, translesion DNA synthesis (TLS), a

102 emoval of FANCD2 from extracts inhibits both nucleolytic incisions near the ICL and translesion DNA s

103 ir, cross-links are resolved ("unhooked") by nucleolytic incisions surrounding the lesion.

104 Thus, BRCA2 prevents rather than repairs nucleolytic lesions at stalled replication forks to main

105 sm of SAMHD1 restriction and argue against a nucleolytic mechanism, which would not be reversible.

106 of damage to coordinate repair that includes nucleolytic modification of the DNA surrounding the lesi

107 ear the 3' end of truncated mRNA; therefore, nucleolytic mRNA processing is required before paused ri

108 airpin-sealed DNA (coding) ends that require nucleolytic opening before their repair by classical non

109 istinguish among several recombinational and nucleolytic pathways, we developed a novel physical assa

110 ATP-dependent DNA end recognition and nucleolytic processing are central functions of the Mre1

111 Therefore, nucleolytic processing by Mre11 is an essential function

112 e-like structural intermediate that requires nucleolytic processing by SbcC-SbcD and ExoI before chro

113 the roles of Mre11 in both DNA bridging and nucleolytic processing during initiation of DSB repair,

114 Biogenesis of miRNAs involves nucleolytic processing of a precursor transcript with ex

115 tranded DNA (ssDNA) that is derived from the nucleolytic processing of a primary lesion.

116 Nucleolytic processing of chromosomal DNA is required in

117 Nucleolytic processing of DNA double-strand breaks (DSBs

118 e RAD50 and MRE11 genes are required for the nucleolytic processing of DNA double-strand breaks.

119 ng DNA ends and ATM activation, but prevents nucleolytic processing of DNA ends, while ATP hydrolysis

120 in 1 (53BP1) protects the genome by limiting nucleolytic processing of DSBs by a mechanism that requi

121 , RET1's TUTase activity is required for the nucleolytic processing of gRNA, rRNA, and mRNA precursor

122 ANCD2 monoubiquitination to determine if the nucleolytic processing of ICLs is required for the activ

123 uggest that polyadenylation occurs after the nucleolytic processing of primary transcripts and in som

124 s occurs as a consequence of MRE11-dependent nucleolytic processing of reversed forks generated by fo

125 and that this may involve, at least in part, nucleolytic processing of telomeric G4.

126 ster chromatid exchange, both arising out of nucleolytic processing of telomeric homologous recombina

127 group D2 (FANCD2) for the initiation of the nucleolytic processing of the DNA cross-links and stabil

128 We propose that nucleolytic processing of unusual replication intermedia

129 is made at the level of DNA end resection, a nucleolytic processing step, which primes DSBs for repai

130 uggest that the NHEJ alignment step precedes nucleolytic processing steps in a significant fraction o

131 combination reaction is composed of multiple nucleolytic processing steps mediated by the recombinati

132 staggered in the T tract and is followed by nucleolytic processing to generate the mature 3' end.

133 only at coding ends that have not undergone nucleolytic processing, this observation being the basis

134 ay have previously unrecognized roles in DNA nucleolytic processing.

135 epeats can occur at ends that have undergone nucleolytic processing.

136 were found at these junctions, demonstrating nucleolytic procession of the viral DNA; however, the la

137 blunt ends can be reconciled with the other nucleolytic properties of both Artemis and Artemis.DNA-P

138 For this reason, we further studied the nucleolytic properties of PALF, and we searched for any

139 rocessed by the Mre11-Rad50-Nbs1(Xrs2) (MRN) nucleolytic protein complex in association with the Tel1

140 or other DNA adducts via an Mre11-dependent nucleolytic reaction.

141 , purified RecBCD enzyme has two alternative nucleolytic reactions, depending on the reaction conditi

142 eaving engineered synthetic ribozymes as RNA nucleolytic reagents and as subjects for enzyme kinetics

143 on complexes, patrolling for those requiring nucleolytic rescue, and its short residence time minimiz

144 Nucleolytic resection of DNA double-strand breaks (DSBs)

145 Nucleolytic resection of DNA double-strand breaks is the

146 ng this process, BRCA1-BARD1 facilitates the nucleolytic resection of DNA ends to generate a single-s

147 e show that RecJ nuclease alone can initiate nucleolytic resection of DNA with 5'-ssDNA overhangs, an

148 coupling factor between presynapsis and the nucleolytic resection of double-stranded DNA ends.

149 Nucleolytic resection of DSBs generates long 3' single-s

150 donuclease 2) nuclease participates in 3'-5' nucleolytic resection of oxidative DNA damage and activa

151 k repair by homologous recombination entails nucleolytic resection of the 5' strand at break ends.

152 n contrast, homologous recombination entails nucleolytic resection of the 5'-strands, forming 3'-ssDN

153 nclude all of the features observed in vivo (nucleolytic resection, P nucleotides, and N nucleotide a

154 proteins have been performed to evaluate the nucleolytic resection, polymerization, and ligation step

155 esting a requirement of the SLX4 complex for nucleolytic resolution of branched intermediates during

156 y step in meiotic recombination involves the nucleolytic resolution of Holliday junctions to generate

157 The possible role of nucleolytic retraction in disengaging pol III from TFIII

158 The VS nucleolytic ribozyme has a core comprising five helices

159 The twister RNA is a recently discovered nucleolytic ribozyme that is present in both bacteria an

160 Nucleolytic ribozymes catalyze site-specific cleavage of

161 Small self-cleaving nucleolytic ribozymes contain catalytic domains that acc

162 own mechanistic strategies employed by small nucleolytic ribozymes.

163 esis of primary piRNAs involves at least two nucleolytic steps.

164 intermediates that are subject to additional nucleolytic trimming to render mature crRNAs of specific