ライフサイエンスコーパス: nucleosome assembly

1 required for efficient DNA synthesis-coupled nucleosome assembly.

2 tin and the proteins required for new CENP-A nucleosome assembly.

3 eosomes, while the reverse reaction promotes nucleosome assembly.

4 Rtt109, the H3 lysine 56 KAT, to promote RC nucleosome assembly.

5 s only moderate effects on H3-H4 binding and nucleosome assembly.

6 to CAF-1, a histone chaperone involved in RC nucleosome assembly.

7 n of the N-terminal residues of H3 and H4 in nucleosome assembly.

8 way, and enters the nucleus where it impedes nucleosome assembly.

9 ces of genome-scale, replication-independent nucleosome assembly.

10 leosome positioning sequences favored during nucleosome assembly.

11 stent with their role in replication-related nucleosome assembly.

12 AF-1*H3-H4 binding mode and the mechanism of nucleosome assembly.

13 t, the substrate for replication-independent nucleosome assembly.

14 CAT interruptions in (CAG)n tracts decreased nucleosome assembly.

15 nes to regulate the thermodynamic process of nucleosome assembly.

16 anded DNA, has a role in replication-coupled nucleosome assembly.

17 storage, sperm chromatin decondensation, and nucleosome assembly.

18 H3 or H4 is required for replication-coupled nucleosome assembly.

19 ot equalCG in terms of facilitating archaeal nucleosome assembly.

20 for FACT to deposit histones onto DNA during nucleosome assembly.

21 hromatin assembly factor-1 (CAF-1)-dependent nucleosome assembly.

22 in egg extract under conditions that titrate nucleosome assembly.

23 H3-H4)2 tetramers during replication-coupled nucleosome assembly.

24 nized these lysines in yeast and assayed for nucleosome assembly.

25 ent human H4 is therefore completed prior to nucleosome assembly.

26 one acetylation conferred by yCAF-I-mediated nucleosome assembly.

27 nvolved in the nuclear import of H2A-H2B and nucleosome assembly.

28 e most favored DNA sequences yet defined for nucleosome assembly.

29 e re-establishes both cellular viability and nucleosome assembly.

30 interphase centromeres to promote new CENP-A nucleosome assembly.

31 tion itself does not directly interfere with nucleosome assembly.

32 formation of the HIRA-H3.3 complex and H3.3 nucleosome assembly.

33 rlying Nap1-mediated H2A-H2B chaperoning and nucleosome assembly.

34 sis for Nap1-mediated H2A-H2B deposition and nucleosome assembly.

35 ith the DNA replication forks and subsequent nucleosome assembly.

36 leosomal loop of DNA, suggesting a means for nucleosome assembly.

37 a cells depends on the function of Rtt106 in nucleosome assembly.

38 Modification of histones H3 and H4 prevented nucleosome assembly.

39 PCNA unloading is delayed in the absence of nucleosome assembly.

40 intain opportunity for CAF-1 recruitment and nucleosome assembly.

41 ependent on new histone supply and efficient nucleosome assembly.

42 are then transferred to other chaperones for nucleosome assembly.

43 re and gene expression are both regulated by nucleosome assembly.

44 en histone acetylation and ubiquitylation in nucleosome assembly.

45 hat Scm3 plays an active role in centromeric nucleosome assembly.

46 , and the 26S proteasome generally, in plant nucleosome assembly.

47 osed to function in replication-coupled (RC) nucleosome assembly, a process critical for the inherita

48 he absence of DNA replication and stimulated nucleosome assembly activity by recombinant yeast CAF-I

49 defective for PCNA binding displayed reduced nucleosome assembly activity in vitro but were stimulate

50 oth in vitro and in vivo, and it displayed a nucleosome assembly activity in vitro.

51 This unanticipated nucleosome assembly activity of AM-PARP-1, coupled with

52 tion is to recruit HJURP and that the CENP-A nucleosome assembly activity of HJURP is responsible for

53 geting domain of Cse4 is sufficient for Scm3 nucleosome assembly activity.

54 protein SET after Lys(176) and disrupted its nucleosome assembly activity.

55 s histone-binding domains and inhibiting its nucleosome assembly activity.

56 ncluding K14 also delayed replication-depend nucleosome assembly and advanced replicative life span.

57 14,000) host genes probed, comprising mainly nucleosome assembly and binding, central nervous system

58 In Wwox(-/-) mice, genes related to nucleosome assembly and cell growth genes were down-regu

59 etrameric state can be used as substrates in nucleosome assembly and chaperone-mediated lysine acetyl

60 As nucleosome assembly and chromatin remodeling are importa

61 1 in H2A/H2B deposition and exchange, during nucleosome assembly and chromatin remodeling in vivo.

62 functional distinction between ATP-dependent nucleosome assembly and chromatin remodeling, and sugges

63 roteins that are central to the processes of nucleosome assembly and disassembly and thus the fluidit

64 nstration of the dynamic equilibrium between nucleosome assembly and disassembly at the single chroma

65 on during Pol II elongation, suggesting that nucleosome assembly and disassembly occur in a stepwise

66 hought to be the first and the last steps in nucleosome assembly and disassembly, respectively.

67 by facilitating histone exchange as well as nucleosome assembly and disassembly.

68 s implicated in histone shuttling as well as nucleosome assembly and disassembly.

69 e in escorting histones to and from sites of nucleosome assembly and disassembly.

70 sition in the nucleosome with regard to both nucleosome assembly and gene regulation.

71 itates Chromatin Transcription) functions in nucleosome assembly and H2A-H2B deposition during transc

72 rk for understanding how Vps75 mediates both nucleosome assembly and histone acetylation by Rtt109.

73 DNA-binding) proteins regulate ATP-dependent nucleosome assembly and mobilization through their conse

74 vitro but, surprisingly, is dispensable for nucleosome assembly and only makes a modest contribution

75 of CGG repeat expansion and methylation for nucleosome assembly and positioning on the Fragile X Men

76 the consequences of CTG repeat expansion for nucleosome assembly and positioning.

77 uitylated by the Rtt101(Mms1) ligase impairs nucleosome assembly and promotes Asf1-H3 interactions.

78 egulate HIRA-H3.3 complex formation and H3.3 nucleosome assembly and reveal the mechanism by which OG

79 dea that the effects of the spt mutations on nucleosome assembly and/or stability activate repressors

80 odifications that accompany DNA replication, nucleosome assembly, and H2A/H2B exchange were examined

81 nucleosomes, the machinery that accomplishes nucleosome assembly, and the functional organization of

82 ults also indicate that the slowest steps in nucleosome assembly are DNA termini wrapping and tetrame

83 ponding lysine acetyltransferase (KAT) in RC nucleosome assembly are not known.

84 Further, by using nucleosome assembly assays, we demonstrate that these tw

85 shed light on the mechanism of Scm3-mediated nucleosome assembly at the centromere.

86 This approach resulted in nucleosome assembly beginning immediately downstream of

87 e that H3.3-specific residues involved in RI nucleosome assembly-but not major histone modification s

88 However, nucleosome assembly by hyperacetylated histones on inter

89 This is an example of selective nucleosome assembly by Xenopus nuclear extracts on a sho

90 ing transcription regulation (TIF1alpha) and nucleosome assembly (CAF1).

91 NAPs have been reported to have a role in nucleosome assembly, cell cycle regulation, cell prolife

92 s, both interruptions permit a propensity of nucleosome assembly closer to that of random (geneticall

93 Thus, the HIR complex is a novel nucleosome assembly complex which functions with SWI/SNF

94 on onto DNA in vitro and constitutes a novel nucleosome assembly complex.

95 es were found to direct a similar pattern of nucleosome assembly, consistent with the view that nucle

96 ere with Nap1 oligomerization exhibit severe nucleosome assembly defects showing that oligomerization

97 How the very first step in nucleosome assembly, deposition of histone H3-H4 as tetr

98 simple, intrinsically stochastic process of nucleosome assembly, disassembly, and position-specific

99 to a simple model involving a noncooperative nucleosome assembly/disassembly equilibrium, suggesting

100 Here, we show that the nucleosome assembly/disassembly process is not strictly

101 amic packaging of the genome by carrying out nucleosome assembly/disassembly, histone exchange, and n

102 ocalization patch in addition to loss of the nucleosome assembly domain.

103 these CGG repeats abolishes the preferential nucleosome assembly due to methylation.

104 H3, has been implicated in the regulation of nucleosome assembly during DNA replication and repair, a

105 ative system could communicate the degree of nucleosome assembly during DNA replication and the need

106 Surprisingly, PIP2 is essential for nucleosome assembly during DNA replication in vitro and

107 uring the cell cycle to provide histones for nucleosome assembly during DNA replication.

108 lated at lysine 56 (H3K56ac) and facilitates nucleosome assembly during several molecular processes.

109 d biochemically tractable when compared with nucleosome assembly during simian virus 40 (SV40) DNA re

110 sstalk between MMR and replication-dependent nucleosome assembly during the correction of DNA replica

111 mbedded centromeres by blocking conventional nucleosome assembly early in S phase, thereby allowing t

112 t the GAA GAA TTC triplex further lowers the nucleosome assembly efficiency (82% decrease compared to

113 lly similar functions of H2B ubiquitylation (nucleosome assembly) elicit different functional outcome

114 ES cells lacking the nucleosome assembly factor HirA exhibit elevated levels

115 ASF1 is a nucleosome assembly factor which is a member of the H3/H

116 replication initiation factor, securin gene, nucleosome assembly factor, and a subunit of the cohesin

117 show that Scm3 functions as a Cse4-specific nucleosome assembly factor, and that the resulting octam

118 chaperone Scm3 functions as a Cse4-specific nucleosome assembly factor.

119 teract with multiple DNA replication-coupled nucleosome assembly factors, including Rtt106, CAF-1, an

120 Nucleosome assembly following DNA replication and gene t

121 Nucleosome assembly following DNA replication controls e

122 ly-distributed nucleosomes are formed by the nucleosome assembly function of Chd1, and then regularly

123 one variant Cse4 to centromeres, its role in nucleosome assembly has not been tested.

124 s, plasmid superhelicity in whole cells, and nucleosome assembly in cell extracts.

125 nt protein regulates DNA replication-coupled nucleosome assembly in part through regulating histone-h

126 play an important but redundant function in nucleosome assembly in the budding yeast, Saccharomyces

127 ed understanding of the factors that mediate nucleosome assembly in the nascent male pronucleus, the

128 ng to the results, CpG methylation expedites nucleosome assembly in the presence of abundant DNA and

129 bited upon deletion of Hira due to a lack of nucleosome assembly in the sperm genome.

130 Nucleosome assembly in the wake of DNA replication is a

131 reased expression of histones or a defect in nucleosome assembly in the yeast Saccharomyces cerevisia

132 similar to NAP-1, NLP, and Nph, facilitates nucleosome assembly in vitro and is therefore a histone

133 ional and rotational positioning of archaeal nucleosome assembly in vitro by a sequence from the 7S R

134 al region of yNAP1, although dispensable for nucleosome assembly in vitro, contributes to binding via

135 ent with these findings, Spt6p is capable of nucleosome assembly in vitro.

136 ToRC facilitates ATP-dependent nucleosome assembly in vitro.

137 We also analyzed nucleosome assembly in vivo and in vitro by examining pl

138 the histone amino termini are important for nucleosome assembly in vivo and in vitro.

139 Nucleosome assembly in vivo requires assembly factors, s

140 The histone H4 mutants are competent for nucleosome assembly in vivo, and the residues that are a

141 During nucleosome assembly in vivo, newly synthesized histone H

142 Therefore, K5/K12 cannot be required for nucleosome assembly in yeast.

143 e results demonstrate that Gcn5 regulates RC nucleosome assembly, in part, by promoting H3 associatio

144 ctural components of H3 and H4 necessary for nucleosome assembly into metazoan chromosomes in vivo.

145 Nucleosome assembly is a highly regulated process that r

146 Replication-coupled nucleosome assembly is a major step in packaging the new

147 The first step in replication-coupled nucleosome assembly is CAF-1-dependent histone (H3-H4)2

148 one chaperones have been identified, but how nucleosome assembly is coupled to DNA replication remain

149 stem for the analysis of replication-coupled nucleosome assembly is described.

150 DNA replication-coupled nucleosome assembly is essential to maintain genome inte

151 Our results reveal that replication-coupled nucleosome assembly is necessary to generate a bilateral

152 In this "two-step system," nucleosome assembly is performed in a separate reaction

153 Because the nucleosome assembly is performed separately from the DNA

154 transcription initiation, on which archaeal nucleosome assembly is positioned downstream from the si

155 They show that defects in nucleosome assembly lead to DNA double-strand breaks and

156 employed to suppress kinetic bottlenecks in nucleosome assembly, lead to cooperative behavior that c

157 derstanding of the mechanisms of centromeric nucleosome assembly, maintenance, and reorganization rem

158 ypothesized that the information controlling nucleosome assembly may coincide with the information th

159 which H3K56Ac regulates replication-coupled nucleosome assembly mediated by CAF-1 and Rtt106.

160 We investigated nucleosome assembly mediated by histone chaperone Nap1 a

161 clear transport, nucleotide excision repair, nucleosome assembly, membrane trafficking, and cytoskele

162 e examined the effects of these drugs on the nucleosome assembly of DNA molecules that display differ

163 chanism whereby H4S47ph distinctly regulates nucleosome assembly of H3.1 and H3.3.

164 ts indicate that HAT1 differentially impacts nucleosome assembly of H3.1-H4 and H3.3-H4.

165 nucleosome assembly of H3.3-H4 and inhibits nucleosome assembly of H3.1-H4 by increasing the binding

166 7ph), catalyzed by the PAK2 kinase, promotes nucleosome assembly of H3.3-H4 and inhibits nucleosome a

167 How HIRA-mediated nucleosome assembly of H3.3-H4 is regulated remains not

168 racts with UBN1, modifies HIRA, and promotes nucleosome assembly of H3.3.

169 ble) sequences, suggesting an association of nucleosome assembly of trinucleotide repeats and genetic

170 ective in H3-H4 binding exhibited attenuated nucleosome assembly on nascent chromatin.

171 as a model system to determine the effect of nucleosome assembly on nucleotide excision repair (NER)

172 to increased and decreased propensities for nucleosome assembly on the (CAG)n and (CGG)n repeats, re

173 omes and by an increase in the efficiency of nucleosome assembly on the injected plasmid.

174 We have used magnetic tweezers to study nucleosome assembly on topologically constrained DNA mol

175 verify our hypothesis that the lack of full nucleosome assembly on topologically constrained tethers

176 The FRA16B DNA fragments strongly exclude nucleosome assembly only in the presence of distamycin,

177 , it was shown that H2Bub1 was important for nucleosome assembly onto nascent DNA at active replicati

178 sites are not necessary for CAF-1-dependent nucleosome assembly onto replicated DNA.

179 hat CAC is a key intermediate of the de novo nucleosome assembly pathway and that the p48 subunit par

180 zed histones in this DNA replication-coupled nucleosome assembly pathway remain enigmatic.

181 ors influence histone nuclear import and the nucleosome assembly pathway, leading to changes in chrom

182 e histone octamer, with implications for the nucleosome assembly pathway.

183 ote the entry of histones H3 and H4 into the nucleosome assembly pathway.

184 p among modifications, histone variants, and nucleosome assembly pathways is unclear.

185 ne modifications are tied to the alternative nucleosome assembly pathways that use primarily H3 at re

186 th histone H4, into nucleosomes via distinct nucleosome assembly pathways.

187 nascent DNA, Okazaki fragment processing and nucleosome assembly potentially affect one another.

188 B dimers was generally poor, suggesting that nucleosome assembly precedes and inhibits HSF binding.

189 ost probably in the form of twist) stall the nucleosome assembly process.

190 H4K5,12ac differentially regulate these two nucleosome assembly processes.

191 Concomitant nucleosome assembly protects the discontinuous mismatch-

192 The nucleosome assembly protein (NAP) family represents a ke

193 This gene is homologous to yeast nucleosome assembly protein (NAP1) and to a human homolo

194 cribe the cloning and analysis of Drosophila nucleosome assembly protein 1 (dNAP-1), a core histone-b

195 emodeling factor (ACF), purified recombinant nucleosome assembly protein 1 (dNAP1), purified native c

196 re we show that the acidic histone chaperone nucleosome assembly protein 1 (NAP-1) from yeast reversi

197 On separation of nuclear proteins, nucleosome assembly protein 1 (Nap-1) has an overlapping

198 Nucleosome assembly protein 1 (NAP-1) is an integral com

199 coactivator p300, we obtained cDNAs encoding nucleosome assembly protein 1 (NAP-1), a 60-kDa histone

200 Homologues of nucleosome assembly protein 1 (NAP1) are found throughou

201 found that the cytoplasmic histone chaperone nucleosome assembly protein 1 (Nap1) associates with the

202 The histone chaperone nucleosome assembly protein 1 (NAP1) is implicated in hi

203 d adding an excess of the histone chaperone, nucleosome assembly protein 1 (NAP1) to the H3/H4 prior

204 We find that the histone chaperone, nucleosome assembly protein 1 (NAP1), cooperates with CB

205 Histone chaperones, like nucleosome assembly protein 1 (Nap1), play a critical ro

206 mechanism of action of the histone chaperone nucleosome assembly protein 1 (Nap1).

207 actors, including histone chaperones such as nucleosome assembly protein 1 (Nap1).

208 of chromatin (RSC) and the histone chaperone nucleosome assembly protein 1 (NAP1).

209 Here we demonstrate that nucleosome assembly protein 1 (Nap1p), a protein previou

210 The self-association properties of the yeast nucleosome assembly protein 1 (yNAP1) have been investig

211 The yeast nucleosome assembly protein 1 (yNAP1) participates in ma

212 The yeast nucleosome assembly protein 1 (yNap1) plays a role in ch

213 similar overall architecture with the yeast nucleosome assembly protein 1 and human SET/TAF-1beta/IN

214 3/H4 during their deposition on DNA by NAP1 (nucleosome assembly protein 1).

215 helicase-interacting protein 1), along with nucleosome assembly protein 1, as novel ubiquitin-intera

216 Several unbiased screens identified nucleosome assembly protein 1-like 1 (NAP1L1) as an inte

217 This factor, called nucleosome assembly protein 1-like 1 (NAP1L1), is a nucl

218 Here, we show that nucleosome assembly protein 1-like protein 1 (NAP1L1) as

219 mber of cellular proteins, in particular the nucleosome assembly protein 1-like protein 1 (NAP1L1), b

220 ion vehicle used for this analysis was NAP1, nucleosome assembly protein 1.

221 ue of NAP1, and we designate it hNAP2 (human nucleosome assembly protein 2).

222 a peptide domain with strong homology to the nucleosome assembly protein family.

223 The nucleosome assembly protein Nap1 is used extensively in

224 GzmA cleaved the nucleosome assembly protein SET after Lys(176) and disru

225 ch contains three granzyme A substrates, the nucleosome assembly protein SET, the DNA bending protein

226 ociated complex (SET complex) containing the nucleosome assembly protein SET, the tumor suppressor pp

227 es, two histone modification proteins, and a nucleosome assembly protein were detected, clearly indic

228 xNAP1 (Xenopus nucleosome assembly protein) belongs to the family of nu

229 ompared the abilities of dNLP and Drosophila nucleosome assembly protein-1 (dNAP-1) to promote the de

230 release that was enhanced by the presence of nucleosome assembly protein-1 (NAP1).

231 mbly and remodeling factor (ACF), Drosophila nucleosome assembly protein-1, plasmid DNA, and ATP.

232 interacts with nap1, which encodes the NAP-1 nucleosome assembly protein.

233 TSPYL2 is an X-linked gene encoding a nucleosome assembly protein.

234 Nucleosome assembly proteins (Nap proteins) represent a

235 e assembly protein) belongs to the family of nucleosome assembly proteins (NAPs) and shares 92% ident

236 e 6 and encodes a protein with homology with nucleosome assembly proteins (NAPs); it has tissue-speci

237 ny factors, including the four HIR genes and nucleosome assembly proteins Asf1p and chromatin assembl

238 identified an mRNA, belonging to a family of nucleosome assembly proteins, whose expression is upregu

239 coupling of fork speed and PCNA unloading to nucleosome assembly provides a simple mechanism to adjus

240 IPs are capable of preferentially inhibiting nucleosome assembly, rather than DNA synthesis, suggesti

241 We present evidence for a paradigm in which nucleosome assembly requires the elimination of competin

242 CENP-A nucleosome assembly requires the Mis18 complex and the C

243 CENP-A nucleosome assembly requires the three-protein Mis18 com

244 ate DNA-synthesis-dependent and -independent nucleosome assembly, respectively.

245 acts occur during concomitant nick-dependent nucleosome assembly shaped by the replication histone ch

246 eosomes replicated in the absence of de novo nucleosome assembly showed that histone modifications do

247 lates one of two Arabidopsis homologs of the nucleosome assembly/silencing protein Asf1 and histone H

248 block is 4.9 +/- 0.6-fold less efficient in nucleosome assembly than a similar length pUC19 fragment

249 -fold and 2.1 +/- 0.3-fold less efficient in nucleosome assembly than the respective unmethylated for

250 o aid in understanding the thermodynamics of nucleosome assembly, the equilibrium stabilities of the

251 Indeed, oocytes lack any measurable CENP-A nucleosome assembly through the entire fertile lifespan

252 at as few as six CTG repeats will facilitate nucleosome assembly to a similar extent as the 50 or mor

253 iation suggests that HMG2 may facilitate the nucleosome assembly, transcriptional activation, and DNA

254 Nucleosome assembly was assayed by three methods.

255 Here, we investigate the mechanism of nucleosome assembly with a purified chromatin assembly s

256 replication fork, through which RPA couples nucleosome assembly with ongoing DNA replication.

257 l cycle surveillance mechanism that monitors nucleosome assembly without involving the DNA repair pat