ライフサイエンスコーパス: nucleotide sequencing

1 tic analysis (FINS, Forensically Informative Nucleotide Sequencing).

2 The PCR product was subjected to nucleotide sequencing.

3 transcription-polymerase chain reaction and nucleotide sequencing.

4 tive 9.4-kb clone, pUEF728, was subjected to nucleotide sequencing.

5 hybridization with a hifC gene probe, and by nucleotide sequencing.

6 sertions within the genes were determined by nucleotide sequencing.

7 , offers a rapid and accurate alternative to nucleotide sequencing.

8 followed by heteroduplex analysis and direct nucleotide sequencing.

9 ere then compared by gel electrophoresis and nucleotide sequencing.

10 ingle-base mismatches that were confirmed by nucleotide sequencing.

11 ished by genomic DNA cloning and analyzed by nucleotide sequencing.

12 A1 gene was amplified by RT-PCR, followed by nucleotide sequencing.

13 on, one of these loci (ERK1) was analyzed by nucleotide sequencing.

14 analyzed by heteroduplex mobility assays and nucleotide sequencing.

15 JCV regulatory region underwent cloning and nucleotide sequencing.

16 followed by heteroduplex scanning and direct nucleotide sequencing.

17 cloned, and the mutations were determined by nucleotide sequencing.

18 tein-truncation assay, followed by automated nucleotide sequencing.

19 followed by heteroduplex analysis and direct nucleotide sequencing.

20 liana has been isolated and characterised by nucleotide sequencing.

21 y heteroduplex analysis and direct automated nucleotide sequencing.

22 y heteroduplex analysis and direct automated nucleotide sequencing.

23 rose gel electrophoresis, Southern blot, and nucleotide sequencing.

24 lymerase chain reaction products, and direct nucleotide sequencing.

25 aracterized by reverse transcriptase-PCR and nucleotide sequencing.

26 s and several of these have been analyzed by nucleotide sequencing.

27 riction mapping, Southern blot analysis, and nucleotide sequencing.

28 the presence of rhinovirus was confirmed by nucleotide sequencing.

29 ence was confirmed by biological cloning and nucleotide sequencing.

30 -transcription polymerase chain reaction and nucleotide sequencing.

31 10 times and was completely characterized by nucleotide sequencing.

32 he virus and host genetic markers by PCR and nucleotide sequencing.

33 oligonucleotide microarray hybridization and nucleotide sequencing.

34 aks (15 from one hospital) were subjected to nucleotide sequencing.

35 ned by using species-specific PCR assays and nucleotide sequencing.

36 determined by reverse transcription-PCR and nucleotide sequencing.

37 microscopy, protein profiling, and complete nucleotide sequencing.

38 ization, polymerase chain reaction (PCR) and nucleotide sequencing.

39 fish ST cDNAs were isolated and subjected to nucleotide sequencing.

40 culture results and were confirmed by direct nucleotide sequencing.

41 olymorphism (SSCP) analysis and confirmed by nucleotide sequencing.

42 nking intronic sequences, followed by direct nucleotide sequencing.

43 for alterations of MAD1L1 by RT-PCR-SSCP and nucleotide sequencing.

44 The results, from 16S rRNA gene nucleotide sequencing (1583 pb accession no HE861352) an

45 cell lines were analysed using either direct nucleotide sequencing (28 cases), denaturing gradient ge

46 romatin immunoprecipitation of OmpR:FLAG and nucleotide sequencing, 43 putative OmpR binding sites we

47 However, Southern blot and nucleotide sequencing analyses identified intact cpb2 op

48 Nucleotide sequencing analysis located mutations to diff

49 significant SSCP shifts underwent automated nucleotide sequencing analysis.

50 restriction fragment length polymorphism and nucleotide sequencing analysis.

51 CA1 mutational analysis, involving automated nucleotide sequencing and a protein-truncation assay, wa

52 Advances in high-throughput nucleotide sequencing and bioinformatics make the study

53 ng phages Gamma and Cherry was determined by nucleotide sequencing and comparative analysis.

54 Nucleotide sequencing and deduced amino acid sequence an

55 Here, we identified these receptors by nucleotide sequencing and demonstrate that LTD(4) recept

56 y, we subjected 11 lung cancer cell lines to nucleotide sequencing and did not detect mutations withi

57 yped in our reference laboratory by means of nucleotide sequencing and extensive phylogenetic analyse

58 Nucleotide sequencing and genetic complementation analys

59 Nucleotide sequencing and phylogenetic analyses were con

60 Nucleotide sequencing and phylogenetic analysis of viral

61 with those for strain characterization using nucleotide sequencing and restriction fragment length po

62 tion assays, and mutations were evaluated by nucleotide sequencing and RNA fingerprinting.

63 Nucleotide-sequencing and multiplexed primer-extension a

64 iption polymerase chain reaction genotyping, nucleotide sequencing, and antigenic characterization me

65 Southern blotting, nucleotide sequencing, and detailed restriction mapping

66 lfish samples, application of nested PCR and nucleotide sequencing, and increased knowledge of NLV ge

67 in reaction-based screening of cDNA library, nucleotide sequencing, and mass spectrometry (electrospr

68 ain reaction-based screening of cDNA library/nucleotide sequencing, and mass spectrometry (reversed-p

69 followed by a competition binding assay and nucleotide sequencing, and mutants were tested for DNA-g

70 nomic DNA followed by heteroduplex analysis, nucleotide sequencing, and restriction site analysis.

71 group analysis using 3 genotyping platforms, nucleotide sequencing, and serologic evaluation was perf

72 The results of an empirical nucleotide-sequencing approach indicate that the evoluti

73 of ENA is to support and promote the use of nucleotide sequencing as an experimental research platfo

74 ferences in human MHC molecules, revealed by nucleotide sequencing but not by serologic typing, subst

75 Consensus nucleotide sequencing confirmed genotype changes in 2 pa

76 Gel electrophoresis and nucleotide sequencing confirmed the authenticity of the

77 Recently, direct nucleotide sequencing confirmed this estimate.

78 perature stress tests in vitro combined with nucleotide sequencing, confirmed this stability but iden

79 lyses were conducted using Sanger population nucleotide sequencing data derived from blood samples fr

80 Southern hybridization and nucleotide sequencing data have revealed apparent csrA h

81 The partners of the International Nucleotide Sequencing Database Collaboration, which incl

82 enomic DNA, heteroduplex analysis and direct nucleotide sequencing demonstrated pathogenetic COL7A1 m

83 followed by heteroduplex analysis and direct nucleotide sequencing, did not reveal sequence variants

84 GSA correlated well with nucleotide sequencing for estimating HCV genetic diversi

85 clone was named "3pmmuc5b-1" after complete nucleotide sequencing (Genbank Accession, AF369933).

86 Nucleotide sequencing has become increasingly common and

87 Nucleotide sequencing has revealed that sid2 is located

88 termination of the number of EPIYA motifs by nucleotide sequencing, however, is a laborious and expen

89 Nucleotide sequencing identified a TonB-dependent recept

90 Single-stranded conformation variance and nucleotide sequencing identified all patient mutations i

91 Nucleotide sequencing identified an open reading frame p

92 Nucleotide sequencing identified atypical cpb2 genes wit

93 Nucleotide sequencing identified several of the clones i

94 Nucleotide sequencing identified three genes located dow

95 tire coding region of CART was determined by nucleotide sequencing in 91 unrelated subjects with seve

96 rmined by methylation-specific PCR (MSP) and nucleotide sequencing in breast cancer cell lines (3/3),

97 In five cases, beta-chain nucleotide sequencing in five selected Vbeta families sh

98 Despite the tremendous drop in the cost of nucleotide sequencing in recent years, many research pro

99 Dramatic increases in the throughput of nucleotide sequencing machines, and the promise of ever

100 Nucleotide sequencing of 1.2 kb of the 5' flanking regio

101 Nucleotide sequencing of 18 mutants enabled assignment o

102 Subcloning, complementation of trp strains, nucleotide sequencing of 5.1 kb and 1.95 kb of DNA and s

103 Partial nucleotide sequencing of additional clones reveals that

104 Additional nucleotide sequencing of both the DNA polymerase gene an

105 Nucleotide sequencing of BV 5.1 TCR CDR3 showed that eac

106 t the genetic level by deletion analysis and nucleotide sequencing of clones that conferred a transmi

107 tic heteroduplex analysis followed by direct nucleotide sequencing of DNA.

108 Junctional region nucleotide sequencing of expanded TCR-Vbeta subsets was

109 Nucleotide sequencing of expressed Vkappas identified sh

110 Nucleotide sequencing of flaA, flaB, and flaC showed tha

111 Direct nucleotide sequencing of H CDR3s showed adult-type N-nuc

112 Nucleotide sequencing of H chain CDR3s of DEX-specific p

113 We performed nucleotide sequencing of HCV isolates from infected indi

114 ntron-exon borders, was determined by direct nucleotide sequencing of human genomic P1 clones.

115 Nucleotide sequencing of insert DNA revealed an open rea

116 Nucleotide sequencing of mobility shifts detected by sin

117 characterization of the receptor and partial nucleotide sequencing of PBR cDNA revealed that the MDA-

118 Nucleotide sequencing of PCR products derived from diffe

119 genotypic evidence of resistance assessed by nucleotide sequencing of protease and reverse transcript

120 e results were supported by purification and nucleotide sequencing of replicative-form DNA and by the

121 Nucleotide sequencing of reverse transcriptase-polymeras

122 ystem based on reverse transcription-PCR and nucleotide sequencing of the 3' half of the genomic regi

123 Following partial nucleotide sequencing of the 4.2-kb insert, a putative o

124 Comparative nucleotide sequencing of the 78 loci of six M. paratuber

125 Nucleotide sequencing of the abnormal polymerase chain r

126 CR of readthrough transcripts and subsequent nucleotide sequencing of the amplified product revealed

127 e was analyzed by RT-PCR, spectratyping, and nucleotide sequencing of the amplified products at diffe

128 Nucleotide sequencing of the Arabidopsis genome is neari

129 Nucleotide sequencing of the AS17 topA allele and studie

130 ze the circulation patterns of HRSV strains, nucleotide sequencing of the C-terminal region of the G

131 Cloning and nucleotide sequencing of the C. fetus gyrA gene in the 2

132 Nucleotide sequencing of the dmcA gene revealed a potent

133 The results of PCR and nucleotide sequencing of the fliD region of eight differ

134 Complete nucleotide sequencing of the gastrointestinal associated

135 dentified by species-specific PCR assays and nucleotide sequencing of the gene encoding ehrlichia 16S

136 the present work, we report the cloning and nucleotide sequencing of the gene kamA for C. subtermina

137 the United States and China were studied by nucleotide sequencing of the hemagglutinin and neuramini

138 Nucleotide sequencing of the highly conserved SMPD3 gene

139 hering revertants thereof were identified by nucleotide sequencing of the p30 gene.

140 Protein and nucleotide sequencing of the paraproteins' variable regi

141 We performed nucleotide sequencing of the parC and gyrA genes and det

142 c DNA, followed by heteroduplex analysis and nucleotide sequencing of the PCR products demonstrating

143 nt primers that will allow amplification and nucleotide sequencing of the phylogenetically useful mat

144 gN and gB genotypes by cloning, followed by nucleotide sequencing of the plasmid DNA and/or restrict

145 Nucleotide sequencing of the promoter region disclosed t

146 Nucleotide sequencing of the promoter revealed the prese

147 Nucleotide sequencing of the reaper insert in virus popu

148 Nucleotide sequencing of the recovered PCR product indic

149 e of human metapneumovirus (HMPV), consensus nucleotide sequencing of the recovered RNA genomes provi

150 This paper reports the cloning and nucleotide sequencing of the S. typhimurium apeE gene as

151 Nucleotide sequencing of the topo IIalpha promoter regio

152 ute flaccid paralysis (AFP) were compared by nucleotide sequencing of the VP1 capsid region (906 nucl

153 Nucleotide sequencing of this cDNA fragment showed it to

154 Nucleotide sequencing of this complementing 1.6-kb regio

155 Nucleotide sequencing of this ORF revealed that the gene

156 germline of 2/60 patients analysed by direct nucleotide sequencing or DGGE, including a non-conservat

157 esolved the isolates better than either porB nucleotide sequencing or typing of the opa gene.

158 roarray, were further validated by bisulfite nucleotide sequencing (P <0.001).

159 Nucleotide sequencing, phenotypic analysis of mutants, a

160 were characterized by using a combination of nucleotide sequencing plus heteroduplex tracking assay o

161 house-keeping loci are assigned directly by nucleotide sequencing, rather than indirectly from the e

162 selective clones by BstNI fingerprinting and nucleotide sequencing revealed 14 distinct scFv fragment

163 Nucleotide sequencing revealed an open reading frame (OR

164 lowed by heteroduplex scanning and/or direct nucleotide sequencing revealed homozygous mutations in t

165 Nucleotide sequencing revealed that at least four unique

166 In this study, nucleotide sequencing revealed that both ends of the ser

167 Nucleotide sequencing revealed that the basis for the in

168 cDNA cloning and nucleotide sequencing revealed that the csrB gene is loc

169 ndividual exons of ITGA6, followed by direct nucleotide sequencing, revealed that the proband was hom

170 A cDNA encoding S3s was isolated, nucleotide sequencing revealing a coding region with 99.

171 , molecular cloning, Southern hybridization, nucleotide sequencing, semiquantitative RT-PCR, and ribo

172 or VLDL-receptor genes was investigated, but nucleotide sequencing showed that no sequences homologou

173 In addition, nucleotide sequencing studies demonstrated that PepB and

174 We conducted mapping and nucleotide sequencing studies of extensive, multi-megaba

175 Nucleotide sequencing studies show that the 567 amino ac

176 s has vastly expanded through advancement in nucleotide sequencing technologies and an increasing foc

177 High-throughput nucleotide sequencing technologies provide large amounts

178 Nucleotide sequencing these cDNAs revealed that the AP-2

179 gnment algorithms, the exponential growth of nucleotide sequencing throughput threatens to outpace bi

180 dition, in a subset of isolates we performed nucleotide sequencing to assess the level of conservatio

181 pig and two feral pigs and characterized by nucleotide sequencing to belong to genotype 3.

182 NA-enrichment methods and massively parallel nucleotide sequencing to comprehensively identify and ty

183 Samples positive for HCoV were submitted for nucleotide sequencing to determine the species.

184 We used TCR beta-chain nucleotide sequencing to gain insight into the adaptive

185 ls, were molecularly cloned and subjected to nucleotide sequencing to identify the recombinant juncti

186 Nucleotide sequencing was performed for a small subset o

187 l sorter analysis, and TCR junctional region nucleotide sequencing was performed on expanded TCR Vbet

188 The performances of two methods of nucleotide sequencing were compared for the detection of

189 Polymerase chain reaction (PCR) and nucleotide sequencing were used to identify the specific

190 PCR cloning and nucleotide sequencing were used to identify the specific

191 vances in molecular biology, microscopy, and nucleotide sequencing will provide the tools to test the

192 PCR assay of paraffin-embedded tissue and nucleotide sequencing with ribosomal ITS1-ITS2 universal