ライフサイエンスコーパス: nucleotidyl

1 Free radical attack on the nucleotidyl C-1' carbon yields 2-deoxyribonolactone (dL)

2 nylyl cyclase, we constructed a model of the nucleotidyl cyclase domain and mutagenized several resid

3 50, which encodes a protein with a class III nucleotidyl cyclase domain, is required for cyclic GMP s

4 volved in blue-light sensing using FAD and a nucleotidyl cyclase domain.

5 Although it is known that ExoY is a soluble nucleotidyl cyclase that increases the cytoplasmic level

6 a permuted histidine-aspartate domain and a nucleotidyl cyclase-like domain, both of which contain s

7 ultracentrifugation, whereas other class III nucleotidyl cyclases are functional dimers.

8 ations in the corresponding regions of human nucleotidyl cyclases disrupt the normal helical domain s

9 ring, and characterization of photoactivated nucleotidyl cyclases that can be used to manipulate cAMP

10 Nucleotidyl cyclases, including membrane-integral and so

11 own to be involved in defining sub-types for nucleotidyl cyclases, protein kinases, lactate/malate de

12 NAD+-dependent nucleotidyl diphosphohexose 4,6-dehydratases which trans

13 osphohexose 4,6-dehydratases which transform nucleotidyl diphosphohexoses into corresponding 4-keto-6

14 echanism through the formation of a covalent nucleotidyl-enzyme intermediate and overall retention of

15 a catalytic domain similar to that of other nucleotidyl-glucose pyrophosphorylases with a carboxy-te

16 es as the nucleophilic catalyst to which the nucleotidyl group is bonded covalently in the covalent i

17 ter, thereby eliminating the 3'-terminal TA4 nucleotidyl group.

18 r catalysis, thus unifying the HIT family as nucleotidyl hydrolases, transferases, or both.

19 NSP2 and the histidine triad (HIT) family of nucleotidyl hydrolases, which in turn has suggested the

20 tous cellular histidine triad (HIT) group of nucleotidyl hydrolases.

21 to the nonbridging phosphoryl oxygens of the nucleotidyl intermediate appear crucial for the formatio

22 ate into its cyclic diphosphate proceeds via nucleotidyl intermediates and is catalyzed by the produc

23 hough the product is analogous to the enzyme-nucleotidyl intermediates isolated during the reactions

24 them to interact with substrates containing nucleotidyl moieties.

25 pectively, intact and processed McC with the nucleotidyl moiety.

26 ultifunctional RNA-binding NSP2 octamer with nucleotidyl phosphatase activity is central to viroplasm

27 bilizing weak interactions that occur during nucleotidyl-protein-primed initiation events in the vira

28 tral domain that contains the active site of nucleotidyl transfer (Lys-231); (iii) a protease-resista

29 structures illuminate the stereochemistry of nucleotidyl transfer and reveal how remodeling of active

30 vitro by D1(1-545)-K260A, which is inert in nucleotidyl transfer but active in gamma-phosphate cleav

31 ranslocated register allowed NTP binding and nucleotidyl transfer but inhibited pyrophosphorolysis an

32 Nucleotidyl transfer by hRev1 is not necessary for mecha

33 common ancestral mechanism of phosphoryl and nucleotidyl transfer can be harnessed to perform seeming

34 e mechanism of DNA polymerase beta-catalyzed nucleotidyl transfer consists of chemical steps involvin

35 Here we show that nucleotidyl transfer depends on two ionizable groups wit

36 Neither nucleobase modification nor nucleotidyl transfer has previously been reported for a

37 Non-template-directed nucleotidyl transfer is also observed when pol beta-DNA

38 , one of the three carboxylates required for nucleotidyl transfer is located on a different beta stra

39 CCl2 to explore leaving-group effects on the nucleotidyl transfer mechanism and fidelity of DNA polym

40 g Rh.dTTP opposite dAP, the templating base, nucleotidyl transfer occurred, but the rate of product f

41 Ribozymes can catalyze phosphoryl or nucleotidyl transfer onto ribose hydroxyls of RNA chains

42 mimic the pyrophosphate leaving group of the nucleotidyl transfer reaction and effectively inhibit RN

43 of DNA polymerases both for catalysis of the nucleotidyl transfer reaction and for base excision.

44 promoter DNA complex crystals to trigger the nucleotidyl transfer reaction and freezing crystals at d

45 Asn564 contact the incoming dNTP during the nucleotidyl transfer reaction as judged by variations in

46 ctural model based on the stereochemistry of nucleotidyl transfer reaction as well as recent crystall

47 The nucleotidyl transfer reaction catalyzed by DNA polymeras

48 rmined the entire free energy profile of the nucleotidyl transfer reaction catalyzed by Pol kappa and

49 ular mechanics calculations for modeling the nucleotidyl transfer reaction in RNase H, clarifying the

50 arried out an extensive investigation of the nucleotidyl transfer reaction mechanism in the well-char

51 tide and a 3' splice site oligonucleotide, a nucleotidyl transfer reaction occurs that mimics the sec

52 gases, play critical roles in the subsequent nucleotidyl transfer reaction that produces the DNA-aden

53 2+) which restored the k(pol) values for the nucleotidyl transfer reaction to near wild-type levels.

54 metal ion to the A site is required for the nucleotidyl transfer reaction to occur, this metal bindi

55 at the catalytic site, thereby allowing the nucleotidyl transfer reaction to take place with little

56 of Arg-61 synergistically contribute to the nucleotidyl transfer reaction, with additional influence

57 the incoming dNTP of RB69 gp43 prior to the nucleotidyl transfer reaction.

58 of the reactive groups of substrates for the nucleotidyl transfer reaction.

59 ic metal binding is the last step before the nucleotidyl transfer reaction.

60 ement prechemistry step occurring before the nucleotidyl transfer reaction.

61 ates of the ternary EDN complex precedes the nucleotidyl transfer reaction.

62 ate of the dNTP, followed by the associative nucleotidyl transfer reaction; this is facilitated by a

63 isotope effects to investigate mechanisms of nucleotidyl transfer reactions in nucleic acids.

64 ments and the stereochemical course of these nucleotidyl transfer reactions.

65 g features are likely requisite elements for nucleotidyl transfer reactions.

66 -PO4 and 3'-OH polynucleotide ends via three nucleotidyl transfer steps involving ligase-adenylate an

67 i at DNA nicks by means of a series of three nucleotidyl transfer steps.

68 ently seals 3'-OH/5'-PO4 RNA nicks via three nucleotidyl transfer steps.

69 ia an ATP-dependent pathway comprising three nucleotidyl transfer steps: reaction of Rtc with ATP to

70 ylation reactions follow the same pathway of nucleotidyl transfer through a covalent aprataxin-(His14

71 d Mn2+, X-ray structural analysis shows that nucleotidyl transfer to the primer 3'-OH takes place dir

72 merases, by probing leaving group effects on nucleotidyl transfer using a series of dGTP bisphosphona

73 of triphosphate RNA ends as an acceptor for nucleotidyl transfer when gamma-phosphate cleavage is ra

74 lytic residue Asp192, dNTP, and template for nucleotidyl transfer, effectively assembling the active

75 termediate, they illuminate the mechanism of nucleotidyl transfer, especially the stereochemical tran

76 ) complex-highlight a two-metal mechanism of nucleotidyl transfer, whereby (i) an enzyme-bound "catal

77 ive site for the subsequent chemical step of nucleotidyl transfer--in contrast to an opening trend wh

78 only proteins known to catalyze 2'-specific nucleotidyl transfer.

79 sp532, Lys533, and Asn537 in GTP binding and nucleotidyl transfer.

80 synthesis utilizing two metals to facilitate nucleotidyl transfer.

81 active site are not properly positioned for nucleotidyl transfer.

82 of active-site residues in the chemistry of nucleotidyl transfer.

83 /molecular mechanical (QM/MM) studies on the nucleotidyl-transfer reaction catalyzed by the lesion-by

84 The nucleotidyl-transfer reaction coupled with the conformat

85 sing substrate and increasing product of the nucleotidyl-transfer reaction.

86 transfers occur in the transition state for nucleotidyl-transfer reactions catalyzed by RB69 DNA-dep

87 ater molecule is the rate-limiting step, the nucleotidyl-transfer step is associative with a metastab

88 ition which switches to a ring formed by the nucleotidyl transferase (NTase) and OB-fold (OBD) domain

89 losis (Mt-Lig) possesses a unique variety of nucleotidyl transferase activities, including gap-fillin

90 onspecific and template-independent terminal nucleotidyl transferase activity was observed with the B

91 HESO1 exhibits terminal nucleotidyl transferase activity, prefers uridine as the

92 Here we report such a non-canonical 3'-5' nucleotidyl transferase activity.

93 possesses a template-independent 3'-terminal nucleotidyl transferase activity.

94 ted XTUT7 enzyme, which contained solely the nucleotidyl transferase and poly(A) polymerase-associate

95 otype Toprim enzyme that might have had both nucleotidyl transferase and polynucleotide cleaving acti

96 ures reveal a tight docking of the conserved nucleotidyl transferase bi-domain module with a RET1-spe

97 alian nuclear enzyme functions not only as a nucleotidyl transferase but also has a dRP lyase activit

98 The nucleotidyl transferase cGAS, its second-messenger produ

99 complex reveals a unique docking site on the nucleotidyl transferase domain for an 8-amino-acid Pol2

100 cking sites localized to the Cgt1 N-terminal nucleotidyl transferase domain.

101 a), a recently identified, essential nuclear nucleotidyl transferase encoded by two redundant genes,

102 miRNA 3' additions are regulated by multiple nucleotidyl transferase enzymes.

103 e NHEJ activity of Pol4 was dependent on its nucleotidyl transferase function, as well as its unique

104 Here we examined the role of nucleotidyl transferase motif V ((184)LLKMKQFKDAEAT(196)

105 f an N-terminal domain (domain 1, containing nucleotidyl transferase motifs I, III, IIIa and IV) and

106 atalytic residues of Rnl2 are located within nucleotidyl transferase motifs I, IV, and V that are con

107 ctuated by a surface-accessible loop between nucleotidyl transferase motifs III and IIIa.

108 Ceg1p is bound to Cet1p are located between nucleotidyl transferase motifs V and VI.

109 encodes a protein that is classified in the nucleotidyl transferase protein family and was previousl

110 Significantly, the crucial nucleotidyl transferase reaction distance (P(alpha)-O3')

111 DNA polymerases catalyze a metal-dependent nucleotidyl transferase reaction during extension of a D

112 This nucleotidyl transferase reaction required a divalent cat

113 NA capping enzymes are members of a covalent nucleotidyl transferase superfamily defined by a common

114 divergent member of the DNA polymerase beta nucleotidyl transferase superfamily, which includes CCA-

115 nzyme is the smallest member of the covalent nucleotidyl transferase superfamily, which includes the

116 is also the smallest member of the covalent nucleotidyl transferase superfamily, which includes the

117 in the sequence motifs characteristic of the nucleotidyl transferase superfamily.

118 HESO1 encodes a terminal nucleotidyl transferase that prefers to add untemplated

119 nucleotide selection by human Rev1, a unique nucleotidyl transferase that uses a protein template-dir

120 rocessing and 3' nucleotide addition by tRNA nucleotidyl transferase to yield a discrete tRNA-sized m

121 abidopsis, HEN1 suppressor 1 (HESO1, a miRNA nucleotidyl transferase) uridylates 5' fragments to trig

122 n and is catalyzed by the enzyme TRNT1 (tRNA nucleotidyl transferase), which functions in both the cy

123 We also observed that suppression of one nucleotidyl transferase, TUT1, resulted in a global decr

124 iac apoptosis was measured by terminal deoxy-nucleotidyl transferase-mediated dUTP nick end-labeling

125 iac apoptosis was measured by terminal deoxy-nucleotidyl transferase-mediated dUTP nick end-labeling

126 Apoptosis was assessed with terminal nucleotidyl transferase-mediated nick end labeling (TUNE

127 antigen and induction of apoptosis (terminal nucleotidyl transferase-mediated nick end labeling and c

128 sessed by Annexin V, caspase-3, and terminal nucleotidyl transferase-mediated nick end labeling) and

129 angiogenesis (CD31), and apoptosis (terminal nucleotidyl transferase-mediated nick end labeling) were

130 randed cDNA product by use of terminal deoxy-nucleotidyl transferase; (iii) ligation of a DNA linker

131 Ribonuclease H (RNase H) belongs to the nucleotidyl-transferase (NT) superfamily and hydrolyzes

132 and a monomer fold common to members of the nucleotidyl-transferase alpha/beta phosphodiesterase sup

133 nylation complex that includes the conserved nucleotidyl-transferase core of GLD-2 and the N-terminal

134 Arg-155, and Ser-170) within the N-terminal nucleotidyl-transferase domain of Rnl2 and impute specif

135 D-2 thus appears to have evolved specialized nucleotidyl-transferase properties that match the 3' end

136 Ribonuclease H (RNase H) belongs to the nucleotidyl-transferase superfamily and hydrolyzes the p

137 RNase H belongs to a nucleotidyl-transferase superfamily, which includes tran

138 onserved domains with sequence similarity to nucleotidyl transferases (NTs) and acyl transferases and

139 A substrates for isotope effect studies with nucleotidyl transferases and hydrolases.

140 e implications for the evolution of covalent nucleotidyl transferases and virus-host dynamics based o

141 ion blocking the action of 4'-aminoglycoside nucleotidyl transferases by the use of recombinant E. co

142 The TOPRIM domain found in many nucleotidyl transferases contains a DxD motif involved i

143 es, which comprise a superfamily of covalent nucleotidyl transferases defined by a constellation of c

144 e to screen a panel of eight candidate miRNA nucleotidyl transferases for 3' miRNA modification activ

145 NTA following the suppression of a panel of nucleotidyl transferases in cancer cell lines.

146 onserved motifs that define a superfamily of nucleotidyl transferases that act via enzyme-(lysyl-N)-N

147 ars, the characterization and mutagenesis of nucleotidyl transferases that can recognize and couple s

148 These enzymes, unlike other nucleotidyl transferases, catalyze 2'-5', not 3'-5', pho

149 RNA polymerase in the Pol ss superfamily of nucleotidyl transferases, Trf4p, and a zinc knuckle prot

150 alytic mechanism for the 2'- and 3'-specific nucleotidyl transferases.

151 dylation and thus relies on exonucleases and nucleotidyl transferases.

152 otein has domain similarity with other known nucleotidyl transferases.

153 le-domain proteins or fused with the pathway nucleotidyl transferases; the fusion of KDO8PP with the

154 The CCA-adding enzymes [ATP(CTP):tRNA nucleotidyl transferases] catalyze synthesis of the cons

155 The CCA-adding enzymes [ATP(CTP):tRNA nucleotidyl transferases], which catalyze synthesis of t

156 t the ribozyme catalyzes both phosphoryl and nucleotidyl transfers.