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3 ete set of orthogonal 21st synthetase-amber, ochre and opal suppressor tRNA pairs including the first
7 the beta-gal gene as a reporter, that amber, ochre, and opal suppressors derived from the serine and
8 ere assayed for the ability to complement an ochre B mutant and defects in the morphogenetic pathway
9 dere finds as hematite pushes the use of red ochre by (early) Neandertals back in time significantly,
13 re, we have employed this system using a TAA ochre codon reversion assay to examine the fidelity of t
15 single clone that was recovered harbored an ochre mutation in the cI gene after the first 128 amino
16 ght-member tRNA-Tyr gene family suppress the ochre mutation reporter, there are considerable phenotyp
18 sensitive fluorescent reporter gene with an ochre mutation, fluorescence-activated cell sorting of a
19 clone which lacked the DNA downstream of the ochre mutation, pTRKH2::CI-per2, confirmed the phenotype
24 assemblage including grinding stones, ground ochres, reflective additives and ground-edge hatchet hea
25 or tRNA concentration to the point where the ochre-suppressing tRNA(Ser) is in four- to fivefold exce
27 ng as colonies in 3-5 days) are slow-growing ochre suppressor mutants that probably existed in the cu
30 cribe conditions for the import of amber and ochre suppressor tRNAs derived from Escherichia coli ini
31 further of importing a mixture of amber and ochre suppressor tRNAs for the insertion of two differen
32 s to sense at a low frequency while tRNA-Tyr ochre suppressors (SUP-o) arise at a very high frequency
34 allosuppressor alleles examined, an in-frame ochre (TAA) mutation was present in the SUP45 coding reg
38 corated with a spiral groove filled with red ochre, which closely parallels similar marks that San ma
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