ライフサイエンスコーパス: octylglucoside

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1 combinant enzymes using buffers that contain octylglucoside.

2 of CF1 activated by heat, dithiothreitol, or octylglucoside.

3 After extraction with octylglucoside, a membrane protein was isolated from the

4 ame insoluble in Triton X-100 but soluble in octylglucoside after extraction from MDCK cells during e

5 Triton X-100, Nonidet P-40, Brij, Tween, and octylglucoside all inactivated the enzyme.

6 d by solubilizing cationic liposomes with 1% octylglucoside and complexing a DNA plasmid with the lip

7 Fyn/zeta interaction was sensitive to octylglucoside and filipin, agents that disrupt membrane

8 S-6His complexes purified in the presence of octylglucoside and lipids migrate in a high-resolution g

9 nts tolerate short-chain detergents, such as octylglucoside and nonylglucoside, which are ideal for c

10 nese hamster ovary cells were solubilized in octylglucoside and separated by tandem anion exchange an

11 xtracted from membranes with two detergents: octylglucoside and Triton X114.

12 ding activity could be solubilized with 0.1% octylglucoside; apparent molecular weight Mapp approxima

13 ll-known that short chain detergents such as octylglucoside are more denaturing than long chain deter

14 ture, but treatment at pH </=7 (or with beta-octylglucoside at pH 8.0) caused it to convert to an SDS

15 nts exhibited diverse patterns of native (no octylglucoside) ATPase activity and a range of defects i

16 ssium phosphate salts and the detergent beta-octylglucoside (betaOG) over a wide range of composition

17 58 degrees C and purified in the presence of octylglucoside by sucrose gradient centrifugation and io

18 This was not observed in octylglucoside containing phospholipid.

19 nt to TX-100 extraction but was sensitive to octylglucoside detergent extraction, indicating that HA,

20 integrin alpha 6 beta 1 is isolated from an octylglucoside extract of fibroblasts on T1-coupled Affi

21 dimethylammonio]-1-propanesulfonate [CHAPS], octylglucoside) extraction, suggesting that M1 and NP we

22 ns by sequential, exhaustive extraction with octylglucoside followed by Triton X114 suggested the exi

23 ts new avenues to develop milder versions of octylglucoside for receptor crystallization.

24 al events in the destabilization of GPCRs by octylglucoside: (i) highly mobile detergent molecules fo

25 alpha-helix in trifluoroethanol and in 0.80% octylglucoside in water, respectively.

26 e interaction between the peptide and DNA in octylglucoside is driven by electrostatic forces, and pe

27 tergents such as sodium dodecyl sulfate or n-octylglucoside is that the detergent properties that int

28 nsmembrane helical protein bacteriorhodopsin-octylglucoside micelle and the empty nanodisc (MSP1D1-Nd

29 dopsin can be effectively liberated from the octylglucoside-micelle by collisional (Q-ToF and FT-ICR)

30 Bacteriorhodopsin can be released from the octylglucoside-micelle efficiently on all three instrume

31 In contrast, A2AR mutants in either octylglucoside or nonylglucoside showed decreased alpha-

32 its by half the actin nucleation capacity of octylglucoside-permeabilized and activated WAS platelets

33 sis of microsomal fractions solubilized with octylglucoside revealed that epitope-tagged Cf-4 protein

34 10(6) Da, while under identical conditions, octylglucoside-solubilized activity remained associated

35 tions, the activity of the Triton X-100- and octylglucoside-solubilized material could be partially r

36 Characterization of the octylglucoside-solubilized receptor by sedimentation equ

37 Octylglucoside-solubilized rhodopsin was incorporated by

38 elical structure in trifluroethanol or 0.2 M octylglucoside solutions similar to pVIII.

39 All mutants exhibited octylglucoside-stimulated ATPase activity and beta-subun

40 lkaline sodium carbonate and not at all with octylglucoside, suggesting that denaturation of the toxi

41 In n-octylglucoside, the wild-type DGK had a thermal inactiva

42 We therefore used octylglucoside, which does not affect darbufelone in thi

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