ライフサイエンスコーパス: oligo(dT)

1 e by either anti-beta antibodies or poly(dA):oligo(dT).

2 ive stimulators than either oligo(dA)( )()or oligo(dT).

3 ed or underrepresented after purification by oligo(dT).

4 x) is similar to that of gp5/trx on poly(dA)/oligo(dT).

5 both subunits are able to bind an 18-mer of oligo(dT).

6 ation within the pol III termination signal, oligo(dT).

7 o[d(A-T)]), or straight and rigid [oligo(dA).oligo(dT)].

8 rated that DNA-1 had a marked preference for oligo(dT) (100 nM dissociation constant) and required ol

9 gainst either template RNA poly(A) or primer oligo(dT(12)) independently.

10 In this assay, a biotinylated oligo(dT(12)) primer was immobilized onto streptavidin-c

11 f hepatitis C virus polymerase using poly(A)/oligo(dT(12)) that were or were not preannealed.

12 rform preannealing reactions for poly(A) and oligo(dT(12)), making it possible to characterize mechan

13 temperatures (T(m) values), however, poly(A)/oligo(dT(12-18)) is not expected to form stable duplexes

14 A subfraction of oligo(dT) (15%) moved over 10-fold more slowly, suggesti

15 rst consists of an elongation reaction of an oligo(dT)(15) primer into poly(U).

16 f the H-chain of D5 Fab, restored binding to oligo(dT)15 to 60% of DNA-1 levels, whereas H-chain CDR

17 was probed by photochemical cross-linking to oligo(dT)16.

18 using a number of oligonucleotide sequences: oligo(dT)(25) and a 30 bp oligonucleotide derived from b

19 s mixed together with a mixture of 12 phased oligo(dT)25 antisense primers.

20 es of paramagnetic beads, we found Dynabeads Oligo(dT)25 best suited this application.

21 efficiency of the thymidine oligonucleotide, oligo(dT)25, and providing a reliable surface for the am

22 Using 4 oligo(dT) 3' primers and 5 arbitrary decamers as 5' prim

23 poly(A)+ RNA from a starting RNA solution by oligo(dT)30 covalently linked to latex particles, buffer

24 at 50 degrees C for 10 min by using the RNA-oligo(dT)30 hybrid on the latex particles as the templat

25 croscopic translocation rates of 3 nt s(-1) (oligo(dT)), 35 nt s(-1) (oligo(dU)), and 42 nt s(-1) (ol

26 d that a large fraction of both intranuclear oligo(dT) (43%) and oligo(dA) (77%) moves rapidly with a

27 With poly(dA):oligo(dT)50 as substrate, the exonucleolytic products fo

28 present its 3.5 angstrom structure bound to oligo(dT)9, (iii) provide data that implicate the HTH mo

29 Oligo(dT) affinity chromatography revealed that cytosoli

30 etic oligonucleotides, such as oligo(dA) and oligo(dT), also stimulated the ATPase activity of the Mc

31 e populations of mammalian mRNAs purified by oligo(dT) and 5' cap selection with oligonucleotide micr

32 group possesses relatively high affinity for oligo(dT) and may recognize single-stranded DNA ligands

33 ed with the structure of the complex between oligo(dT) and the D355A/E357A KF mutant.

34 to differential display with combinations of oligo(dT) and various arbitrary PCR primers.

35 ant 125-kDa catalytic subunit using poly(dA).oligo(dT) as a template in the absence of proliferating

36 tro processivity assays with excess poly(dA):oligo(dT) as a template, PF-8 stimulated the production

37 th poly(rC).oligo(dG), but not with poly(rA).oligo(dT), as the template/primer.

38 Laser spot photolysis then uncaged the oligo(dT) at a given intranuclear site and the resultant

39 RNase H can even effectively replace oligo(dT)-based methods for standard RNA-seq.

40 B1/2 from glioma C6 cells do not bind to the oligo(dT)-based separation techniques efficiently, sugge

41 The conversion of D5 to a high affinity oligo(dT)-binding Fab occurred only in the presence of D

42 poly(A) lengths or in the elution profile of oligo(dT)-bound targets.

43 taining pcna-79 was unaffected on poly(dA) x oligo(dT) but was dramatically reduced on a natural temp

44 d a large fraction (45%) of the intranuclear oligo(dT), but not oligo(dA), diffusing at slower rates

45 In vivo UV-crosslinking of RNA to RBPs, oligo(dT) capture and mass spectrometry yielded 1,145 di

46 capture (RIC) uses in vivo UV crosslinking, oligo(dT) capture, and proteomics to identify RNA-bindin

47 the presence of poly(A) tracts by binding to oligo(dT) cellulose followed by hybridization with speci

48 ochondrial extract of rat cerebral cortex by oligo(dT)-cellulose affinity chromatography.

49 nfirmed by immunoblotting fractions bound to oligo(dT)-cellulose and separated by rate sedimentation

50 ined further by a useful technique involving oligo(dT)-cellulose chromatography and antisense oligonu

51 oli poly(A) polymerase I and purifying it by oligo(dT) chromatography.

52 ta* showed a processivity of 2-3 on poly(dA).oligo(dT) compared with 5-10 for Poldelta.

53 e achieved by first synthesizing cDNAs using oligo(dT) coupled with magnetic beads which are then rec

54 n was confirmed by screening the products of oligo(dT)-dependent cDNA synthesis.

55 (100 nM dissociation constant) and required oligo(dT) >5 nucleotides in length.

56 ed the diffusion rate in aqueous solution of oligo(dT) hybridized to a large polyadenylated RNA (1.0

57 s on the isolation of polyadenylated RNA via oligo(dT), it will not provide RNA-binding information o

58 Oligo(dT) labeled with chemically masked (caged) fluores

59 constrain productivity and rely on expensive oligo(dT) ligands for target ss-mRNA poly(A) tail hybrid

60 The use of an oligo(dt) magnetic bead protocol to harvest poly(A) mRNA

61 nked protein-RNA complexes are purified with oligo(dT) magnetic beads.

62 rted to blunt-ended, double-stranded cDNA by oligo(dT)-mediated reverse transcription followed by lin

63 NTP were disrupted by adding excess poly(rA)/oligo(dT) or heparin just prior to electrophoresis.

64 asured diffusion rate for much of the slower oligo(dT) population approximated the diffusion rate in

65 The structure shows that DNA-1 binds oligo(dT) primarily by sandwiching thymine bases between

66 These results demonstrate that oligo(dT) primed 5' capture scRNAseq can detect DENV RNA

67 Sequences have been generated from 26 oligo(dT) primed directionally cloned libraries, of whic

68 These include- (a) use of standardized oligo(dT)-primed cDNA pools rather than total RNA as the

69 to incorporate hundreds of nucleotides on an oligo(dT)-primed poly(dA) template with limiting amounts

70 oly(dT) sequences that are remnants from the oligo(dT)-primed reverse transcription of polyadenylated

71 regulated genes as could be detected by the oligo(dT)-primed target alone, in an experiment in which

72 An oligo(dT)-primed target and three LCRs detect twice as m

73 s detected in reactions containing either an oligo(dT) primer alone, or in reactions supplemented wit

74 the sense primer, and a single base anchored oligo(dT) primer is used as an antisense primer in PCR,

75 e current system of gene identification: the oligo(dT) primer widely used for cDNA synthesis generate

76 n be effectively diminished by replacing the oligo(dT) primer with a set of anchored oligo(dT) primer

77 the patterns of internal poly(A) priming by oligo(dT) primer.

78 the oligo(dT) primer with a set of anchored oligo(dT) primers for reverse transcription.

79 should be synthesized by use of the anchored oligo(dT) primers, rather than the oligo(dT) primers, to

80 anchored oligo(dT) primers, rather than the oligo(dT) primers, to diminish the generation of truncat

81 a potential problem: amplifications based on oligo(dT) priming could be sensitive to RNA degradation;

82 changes that are difficult to measure using oligo(dT) priming for target synthesis.

83 ding of locked nucleic acid (LNA)-containing oligo(dT) probes to poly(A) tails to maximize RNA captur

84 tensities were obtained with only 1% as much oligo(dT)-purified mRNA as total RNA, and hence in vitro

85 rming ion pairs with dT5, Arg contributes to oligo(dT) recognition by helping to maintain the structu

86 ity-determining region 3 (HCDR3) of the anti-oligo(dT) recombinant antibody fragment, DNA-1, contribu

87 elements was replaced by two single-stranded oligo(dT)s is unwound by the UL9 protein-ICP8 complex.

88 Using ITC, we find that DrSSB binding to oligo(dT)s with lengths close to the determined site siz

89 We show that sequencing of oligo(dT)-selected polyadenylated mRNAs, without conside

90 mRNAs could be detected on Northern blots of oligo(dT)-selected RNA from receptor-positive HeLa cells

91 Analyses of the protein composition of oligo(dT)-selected UV photocross-linked ER protein-RNA a

92 to the supernatant are separated by a second oligo(dT) selection.

93 described, employing multiple primers with 5'oligo(dT) sequences (MassTags) which serve to mass discr

94 both Hoechst-labeled chromosomes and uncaged oligo(dT) showed that, excluding nucleoli, the poly(A) R

95 that single mtSSB tetramers bind tightly to oligo(dT) single strands containing 32 to 48 residues.

96 late intermediate and is capable of ligating oligo(dT) strands annealed to poly(rA).

97 V-1 reverse transcriptase (RT) on a poly(rA).oligo(dT) template-primer (TP).

98 the three-subunit complex 4-fold on poly(dA)-oligo(dT) template-primer but had no effect on the activ

99 -subunit enzyme retains activity on poly(dA)/oligo(dT) templates but is impaired in its ability to ex

100 omopolymer RNA template-primer, poly(A), and oligo(dT), the RT with altered Leu74Val mutation was les

101 migration were shown by RNase H mapping with oligo(dT) to be due to poly(A) shortening.

102 termini annealed to a template with a longer oligo(dT) tract are not efficiently extended by pol gamm

103 recognizes and pauses at its terminator, an oligo(dT) tract in non-template DNA, terminates 3' oligo

104 so with precision and efficiency on a simple oligo(dT) tract, independent of other cis-elements or tr

105 l gamma to extend a primer through a defined oligo(dT) tract.

106 oters but must rely on fortuitous downstream oligo(dT) tracts for terminator function.

107 HCDR3 residues of DNA-1 in interaction with oligo(dT) was elucidated.

108 The amount of this slower-moving oligo(dT) was greatly reduced if the oligo(dT) was prehy

109 Fluorescein-labeled oligo(dT) was introduced into HeLa cells stably expressi

110 ATPase activity of the Mcm467 complex, where oligo(dT) was more effective than oligo(dA).

111 -moving oligo(dT) was greatly reduced if the oligo(dT) was prehybridized in solution with (unlabeled)

112 ntroduction to cells, presumably because the oligo(dT) was then unavailable for subsequent hybridizat

113 te and the resultant fluorescent, hybridized oligo(dT) was tracked using high-speed imaging microscop

114 g studies in which caged fluorescein-labeled oligo(dT) was used as hybridization probe, and the rate

115 driving the first reverse transcription with oligo(dT) with a T7 RNA polymerase promoter (T7dT) on th