ライフサイエンスコーパス: oligonucleotide primer

1 enched once they are incorporated onto a DNA oligonucleotide primer.

2 the array of PCR products starting from the oligonucleotide primers.

3 tely 2.1 kb) by 'gene walking' using several oligonucleotide primers.

4 a polymerase chain reaction using degenerate oligonucleotide primers.

5 e cDNA generated with oligo-dT and/or random oligonucleotide primers.

6 psis genome sequences to develop appropriate oligonucleotide primers.

7 ersatile method to amplify specific DNA with oligonucleotide primers.

8 t any labeling requirements of the ddNTPs or oligonucleotide primers.

9 ng various combinations of randomly selected oligonucleotide primers.

10 merase chain reaction using random arbitrary oligonucleotide primers.

11 sing an Rh cE cDNA as template and mutagenic oligonucleotide primers.

12 y(A)+ mRNA was performed by using degenerate oligonucleotide primers.

13 nalysis greatly benefits from removal of the oligonucleotide primers (15- and 17-mers in this instanc

14 eoxyribo or ribonucleotide), embedded within oligonucleotide primers 29-30 nucleotides (nt), or great

15 T-PCR experiments using murine gene-specific oligonucleotide primers analyzed the scope and variety o

16 wo species by polymerase chain reaction with oligonucleotide primers anchored in the conserved 5S cod

17 BI Prism 7700 Sequence Detection System with oligonucleotide primers and a fluorescence-labeled probe

18 This assay used oligonucleotide primers and a TaqMan probe targeting the

19 erization of nucleotide precursors using two oligonucleotide primers and an amplification enzyme, as

20 PCR was performed with degenerate oligonucleotide primers and C. subterminale SB4 chromoso

21 Oligonucleotide primers and FAM- and TAMRA-labeled WN vi

22 he Primer3 utility exists for development of oligonucleotide primers and fills that role effectively.

23 Serotype- and group-specific oligonucleotide primers and fluorogenic probes were desi

24 esized HIV-1 (-)-strand strong-stop DNA from oligonucleotide primers and had minimal effect on RNase

25 We designed gene-specific oligonucleotide primers and probes for the measurement o

26 Oligonucleotide primers and probes were designed to dete

27 Oligonucleotide primers and probes were designed to targ

28 erase chain reaction assay and 5-LO-specific oligonucleotide primers and their mutated internal stand

29 nce of the CF-chitinase to design degenerate oligonucleotide primers and to amplify and sequence a PC

30 on into the standing start point of 5'-[32P]-oligonucleotide primer annealed with M13mp19 phage DNA b

31 f macaque with a consensus degenerate hybrid oligonucleotide primer approach.

32 Traditionally, oligonucleotide primers are 5'-(32)P labeled using T4 ki

33 Oligonucleotide primers are predicted automatically, usi

34 templates by 1b-42 NS5B did not require the oligonucleotide primer at high concentrations (>/=50 mic

35 he convenient approaches of either designing oligonucleotide primers at the splice junction or differ

36 eir activity-dependent modification of a DNA oligonucleotide primer attached to the same phage partic

37 A degenerate oligonucleotide primer based upon internal amino acid se

38 Degenerate oligonucleotide primers based on conserved amino acid re

39 actions (PCRs) performed with mixed sequence oligonucleotide primers based on conserved regions.

40 Oligonucleotide primers based on exon-flanking sequences

41 Using PCR and degenerate oligonucleotide primers based on internal peptide sequen

42 he polymerase chain reaction with degenerate oligonucleotide primers based on sequences common to thr

43 Two pairs of oligonucleotide primers based on the 16S rDNA gene were

44 Using oligonucleotide primers based on the deduced DNA sequenc

45 Oligonucleotide primers based on the human heart monocar

46 Oligonucleotide primers based on the murine sequence wer

47 Oligonucleotide primers based on the partial amino acid

48 Nondegenerate oligonucleotide primers based on the porcine cDNA were s

49 Using oligonucleotide primers based on the reported human CLN3

50 With a set of oligonucleotide primers based on the same primer binding

51 creen a mouse pcDNA3 expression library with oligonucleotide primers based on the translated human le

52 Degenerate oligonucleotide primers based upon this information were

53 Degenerate oligonucleotide primers, based on a partial internal ami

54 Degenerate oligonucleotide primers, based on conserved sequences in

55 rases, which are able to extend the attached oligonucleotide primer by incorporating ribonucleoside t

56 Nested PCR with patient IgH allele-specific oligonucleotide primers can detect 1 tumor cell in 10(4)

57 Using a panel of 19 V beta-specific oligonucleotide primers, changes in the level of TCR V b

58 s for the detection of parasite DNA by using oligonucleotide primers COC-1 and COC-2 for PCR amplific

59 eospora spp. and other apicomplexa coccidia, oligonucleotide primers COC-1 and COC-2 were used for PC

60 This COnsensus-DEgenerate Hybrid Oligonucleotide Primer (CODEHOP) strategy has been imple

61 vel HHVs, using consensus-degenerated hybrid oligonucleotide primers (CODEHOP) PCR: no sequence indic

62 lification using COnsensus DEgenerate Hybrid Oligonucleotide Primers (CODEHOPs) has proven to be high

63 quences based on consensus-degenerate hybrid oligonucleotide primers (CODEHOPs).

64 rally occurring microbial populations, using oligonucleotide primers complementary to highly conserve

65 Oligonucleotide primers complementary to the flanking un

66 ntly elongating completely non-telomeric DNA oligonucleotide primers, consisting of natural telomere-

67 s synthesized by a method that uses two long oligonucleotide primers containing primer binding sites

68 CAPS markers for this study, eight pairs of oligonucleotide primers corresponding to five previously

69 1 gene was isolated using PCR and degenerate oligonucleotide primers corresponding to highly conserve

70 Oligonucleotide primers corresponding to Sp3 messenger R

71 mplification of Mortierella alpina cDNA with oligonucleotide primers corresponding to the conserved r

72 reactive 5-iododeoxypyrimidines into the DNA oligonucleotide primer d(T4G4T4G4T4G2) and, upon irradia

73 ymerase chain reaction with isoform-specific oligonucleotide primers demonstrated the presence of the

74 a deduced 36-kDa protein by using degenerate oligonucleotide primers derived from a 30-residue peptid

75 olymerase chain reaction amplification using oligonucleotide primers derived from conserved regions o

76 by polymerase chain reaction with degenerate oligonucleotide primers derived from conserved segments

77 Using oligonucleotide primers derived from rat CD13 cDNA, a mo

78 y polymerase chain reaction using degenerate oligonucleotide primers derived from regions of sequence

79 Oligonucleotide primers derived from sequences shared by

80 T-PCR with mRNA from wheat crown tissue, the oligonucleotide primers derived from the barley cognate

81 The consensus-degenerate hybrid oligonucleotide primer design and seminested or nested P

82 PROBER is an oligonucleotide primer design software application that

83 A single oligonucleotide primer, designated iRepl, was designed t

84 eaction-based approach relying on degenerate oligonucleotide primers designed according to the amino

85 Oligonucleotide primers designed according to the Rx1 ps

86 PCR using oligonucleotide primers designed for a conserved protein

87 In this study, oligonucleotide primers designed for conserved sequences

88 Oligonucleotide primers designed for conserved sequences

89 p samples were analyzed by nested PCR, using oligonucleotide primers designed for the gene encoding t

90 By using oligonucleotide primers designed from peptide sequence i

91 encoding the proteinase was amplified using oligonucleotide primers designed from the 45 kDa N-termi

92 ed with the inclusion in the PCR reaction of oligonucleotide primers designed to amplify a 3016-bp fr

93 eaction (PCR) in the presence of a series of oligonucleotide primers designed to amplify all 7 exons

94 in vertebrate DNA by PCR amplification with oligonucleotide primers designed to hybridize to the TTA

95 Degenerate oligonucleotide primers designed to the putative calmodu

96 aining a polymorphic locus is incubated with oligonucleotide primers (designed to hybridize to the DN

97 y in the mutant hpt gene product, degenerate oligonucleotide primers, designed according to the N- an

98 ples with IsoQuick was amplified by PCR with oligonucleotide primers directed to the DNA polymerase g

99 n RdRp and sensitive to the concentration of oligonucleotide primer directing RNA synthesis.

100 ducing genome complexity by using degenerate oligonucleotide primer (DOP)-PCR and applied this strate

101 e 'random' sequences amplified by degenerate oligonucleotide primer (DOP)-PCR can be precisely mapped

102 Initially, multiple degenerate oligonucleotide primers (DOP) and probes were designed f

103 Degenerate oligonucleotide primers encoding two of the polypeptide

104 ry to accurately measure the masses of short oligonucleotide primers extended by a single dideoxynucl

105 subjected to polymerase chain reaction with oligonucleotide primers for 12 different viruses (cytome

106 ed a method using a single set of degenerate oligonucleotide primers for amplification of the conserv

107 We have designed a series of intronic oligonucleotide primers for amplifying the entire coding

108 Oligonucleotide primers for bile salt-stimulated cholest

109 flower cDNA library by PCR using degenerate oligonucleotide primers for conserved domains of protein

110 In contrast, oligonucleotide primers for hormone-sensitive lipase yie

111 rase chain reaction (PCR), rely on synthetic oligonucleotide primers for initiation of the reaction.

112 N-terminal sequence enabled construction of oligonucleotide primers for PCR and RACE-derived cDNAs f

113 Using degenerate oligonucleotide primers for the NBS region of N and RPS2

114 Oligonucleotide primers for this sequence were used to a

115 loyed 3' RACE PCR, using a highly degenerate oligonucleotide primer, for the facile isolation of a C2

116 Oligonucleotide primers from sequences that flank 224 of

117 ucleic acids by amplification with arbitrary oligonucleotide primers has become popular because it ca

118 se has been observed to add repeats to a DNA oligonucleotide primer in a processive manner, leading t

119 s indicate that telomerase may interact with oligonucleotide primers in a bipartite manner, with the

120 By utilizing degenerate oligonucleotide primers in a reverse transcriptase-coupl

121 d bacterial 16S ribosomal RNA using specific oligonucleotide primers in polymerase chain reaction (PC

122 Telomerase can extend oligonucleotide primers in vitro in a processive fashion

123 of rat liver poly(A)(+) RNA using 5'-derived oligonucleotide primers indicated that the 5' end sequen

124 Degenerate oligonucleotide primers inferred from peptide sequence d

125 An oligonucleotide primer is annealed to the target DNA imm

126 " In immunoRCA, an oligonucleotide primer is covalently attached to an Ab;

127 Primer extension analysis using two 30-mer oligonucleotide primers known to be contained within the

128 This method utilizes oligonucleotide primers labeled with one of three fluore

129 y conventional synthesis at the 5'-end of an oligonucleotide primer of any sequence.

130 , in which we modify the immobilized lawn of oligonucleotide primers of a next-generation DNA sequenc

131 hain reaction of genomic DNA with degenerate oligonucleotide primers, one based on the N-terminal ami

132 -OddCMP from the 3'-end of a single-stranded oligonucleotide primer or a primer annealed with complem

133 es not copy these RNAs unless a suitable DNA oligonucleotide primer or DNA target site is provided.

134 rmed using template mRNA from T560 cells and oligonucleotide primer pairs designed from the published

135 ay consists of six molecular beacons and two oligonucleotide primer pairs, allowing for detection and

136 ypanosoma cruzi proteins by using degenerate oligonucleotide primers prepared against conserved domai

137 ed at the 5' end of a 5' fluorophore-labeled oligonucleotide primer, quenching the primer's fluoresce

138 of a tung seed cDNA library using degenerate oligonucleotide primers resulted in identification of tw

139 ens RNA and casein kinase I isoform-specific oligonucleotide primers resulted in the amplification of

140 Using an oligonucleotide primer set specific for MN-complimentary

141 An oligonucleotide primer specific for a conserved amino ac

142 of the organisms was confirmed by PCR using oligonucleotide primers specific for E. equi and the HGE

143 ial 16S rDNA was amplified using 2 synthetic oligonucleotide primers specific for eubacteria.

144 liver and human cells to perform RT-PCR with oligonucleotide primers specific for nucleus-encoded tRN

145 PCR) using RNA isolated from WIF-B cells and oligonucleotide primers specific for rat ntcp or human N

146 eudogene was identified by inverse PCR using oligonucleotide primers specific for the 5' region of th

147 A set of universal oligonucleotide primers specific for the conserved regio

148 Using RT-PCR with pairs of sense-antisense oligonucleotide primers specific for the various regions

149 and reverse transcriptase PCR (RT-PCR) with oligonucleotide primers specific to the major subunit, a

150 Oligonucleotide primers targeting prp and cps were combi

151 on of a single nucleotide into a short 25/45 oligonucleotide primer-template by pol beta was used to

152 An oligonucleotide primer-template was designed with an 18-

153 of DNA polymerase I and a series of defined oligonucleotide primer/templates.

154 omparable to radiolabeling is achieved using oligonucleotide primers that are 5'-end labeled with inf

155 Specific oligonucleotide primers that differentiate 1302A from RJ

156 In this method, oligonucleotide primers that have different molecular we

157 om these three groups of mutants by PCR with oligonucleotide primers that hybridize to flanking regio

158 ed the binding properties of single-stranded oligonucleotide primers that serve as telomerase substra

159 cDNA and by RT-PCR analysis with a series of oligonucleotide primers that span the entire cDNA for ap

160 Using oligonucleotide primers that specifically amplify human

161 of different Abs, each labeled with a unique oligonucleotide primer, that can be discriminated by a c

162 olymerase chain reaction with two degenerate oligonucleotide primers, the design of which was based o

163 We show here, using only 4 nested oligonucleotide primers, the successful amplification an

164 plished by hybridizing a short complementary oligonucleotide primer to the target and extending the r

165 GSTs are shown to be long enough for use as oligonucleotide primers to amplify adjacent segments of

166 olymerase chain reaction (PCR) cloning using oligonucleotide primers to DNA sequences conserved withi

167 d a PCR approach employing highly degenerate oligonucleotide primers to isolate several Arabidopsis g

168 rformed using previously reported degenerate oligonucleotide primers to the ligand binding domain (LB

169 Degenerate oligonucleotide primers to the N-terminal sequence of th

170 Oligonucleotide primers to the S2 segment of CTF (Florio

171 by PCR of human brain cDNA using degenerate oligonucleotide primers to transmembrane (TM) domains 3

172 ion, in conjunction with a set of degenerate oligonucleotide primers, to identify a subset of HOX gen

173 SOP3v2-generated oligonucleotide primer trios enable analysis of single n

174 polymerase catalytic subunit, interacts with oligonucleotide primers via two uridylate recognition si

175 The PLC-gamma 1 specific antisense oligonucleotide primer was able to inhibit cell prolifer

176 ucleotide at the 3'-end of each CFET-labeled oligonucleotide primer was complementary to a particular

177 A pair of oligonucleotide primers was designed based on the potato

178 tion-based strategy combined with degenerate oligonucleotide primers was employed.

179 A library of CFET-labeled oligonucleotide primers was mixed with biotin-ddNTPs, DN

180 e chain reaction (RT-PCR) with gene-specific oligonucleotide primers was performed to characterize th

181 Using rat sex-determining region-Y-specific oligonucleotide primers, we determined the donor DNA con

182 Using exon-4-specific oligonucleotide primers, we have amplified, cloned, and

183 nucleotides (dNTPs) at the 3'-OH group of an oligonucleotide primer; we term this methodology surface

184 In a complementary strategy, degenerate oligonucleotide primers were designed against highly con

185 Degenerate oligonucleotide primers were designed based on sequence

186 om the purified 175-kDa HARE, and degenerate oligonucleotide primers were designed for reverse transc

187 Consensus-degenerate hybrid oligonucleotide primers were designed from an internal V

188 In this study, oligonucleotide primers were designed from regions of th

189 Single-stranded oligonucleotide primers were designed to allow loop form

190 Oligonucleotide primers were designed to amplify each ex

191 Oligonucleotide primers were designed to amplify specifi

192 5' and 3' rapid amplification of cDNA ends, oligonucleotide primers were designed to amplify the ent

193 acid sequences derived from the 20-kDa band, oligonucleotide primers were designed, two of which prod

194 Oligonucleotide primers were developed for nested PCR ba

195 32P-Radiolabeled RNA oligonucleotide primers were incubated with yPAP in the

196 For each repeat, flanking oligonucleotide primers were synthesized and the polymer

197 Degenerate oligonucleotide primers were used in PCR to amplify a re

198 s obtained, and inosine-containing redundant oligonucleotide primers were used to amplify an 815-base

199 Degenerate oligonucleotide primers were used to amplify by polymera

200 Degenerate oligonucleotide primers were used to amplify conserved p

201 Degenerate oligonucleotide primers were used to obtain a polymerase

202 ide sequences were used to design degenerate oligonucleotide primers which were then used as a first

203 peptides allowed construction of degenerate oligonucleotide primers, which amplified a 551-base pair

204 es the development of appropriately targeted oligonucleotide primers, which necessitates the identifi

205 Three unique SBE oligonucleotide primers, which probe for mutations of cl

206 potato (Solanum tuberosum) cv. Desiree using oligonucleotide primers with sequences which are highly