ライフサイエンスコーパス: oligoribonucleotide

1 are unable to either synthesize or extend an oligoribonucleotide.

2 and 3' flanking RNAs yields the full-length oligoribonucleotide.

3 cleotide substitutions at the 3' pole of the oligoribonucleotide.

4 her synthesize primers de novo nor extend an oligoribonucleotide.

5 ablation of the 5'-most cleavage site on the oligoribonucleotide.

6 ngly in vitro to synthetic poly-U and poly-A oligoribonucleotides.

7 with the formation of several short abortive oligoribonucleotides.

8 roup at specified positions within synthetic oligoribonucleotides.

9 oligonucleotides were bound to unstructured oligoribonucleotides.

10 binds independently to S/R and thiostrepton oligoribonucleotides.

11 times (2 min) for the automated synthesis of oligoribonucleotides.

12 usion of guanosine monophosphate or specific oligoribonucleotides.

13 ligoribonuclease, causes the accumulation of oligoribonucleotides 2 to approximately 4 nt in length,

14 Essentially, 2'- O -methyl oligoribonucleotides (2'OMeRNA) were delivered to the nu

15 a 2-O-methylated phosphorothioated antisense oligoribonucleotide (2OMeAO) designed to promote skippin

16 cific 2'-O-methyl phosphorothioate antisense oligoribonucleotide (2OMeAO).

17 Here we show that the 2'-hydroxyl group of oligoribonucleotide-3'-phosphates can be chemoselectivel

18 An oligoribonucleotide (a 27-mer) that mimics the sarcin/ri

19 However, NMR spectroscopy of an oligoribonucleotide, a 29-mer that mimics the sarcin/ric

20 ransfection of enterocytes with an antisense oligoribonucleotide against miR-122a blocked the TNF-alp

21 Indeed, Hfq bound to the oligoribonucleotides (AGG)(8), (AGC)(8), and the shorter

22 chimeric primases catalyze the synthesis of oligoribonucleotides albeit at a reduced rate and DNA de

23 EF-G also binds to an oligoribonucleotide (an 84-mer) that has the thiostrepto

24 A 17 base oligoribonucleotide analog of the T-arm was equivalent t

25 labeling either the 5' or the 3' end of the oligoribonucleotide and by examining the reaction produc

26 The number of nitrogen atoms in the oligoribonucleotide and fragment ions can readily be det

27 phosphodiester/phosphorothioate 2'-O-methyl-oligoribonucleotides and 6-9- and almost 200-fold more e

28 P6 primase incorporates GTP at the 5'-end of oligoribonucleotides and CMP at the second position.

29 ion sufficient for site-specific cleavage of oligoribonucleotides and complex RNAs.

30 onal stabilizing interactions between capped oligoribonucleotides and eIF4E, which do not occur with

31 nity selection with biotinylated 2'-O-methyl oligoribonucleotides and glycerol gradient fractionation

32 n have triggered a high demand for synthetic oligoribonucleotides and have motivated the development

33 for the identification and quantification of oligoribonucleotides and post-transcriptional modificati

34 of 2'-SCF3 pyrimidine nucleoside containing oligoribonucleotides and the comprehensive investigation

35 livery of RNAs (siRNAs, miRNA or other short oligoribonucleotides) and small-molecule drugs.

36 of kissing and duplex dimer forms of related oligoribonucleotides, and nucleotides C1-G4 and C27-G30

37 the cells with 2'-O-methyl phosphorothioate oligoribonucleotides antisense toward the aberrant donor

38 our in vitro results, we postulate that the oligoribonucleotides are able to prime synthesis of wild

39 2'-O-Ethylene glycol substituted oligoribonucleotides are second-generation antisense inh

40 ological properties of these novel synthetic oligoribonucleotides as antagonists of TLRs 7, 8 and 9 i

41 lthough the latter are effective in cleaving oligoribonucleotides, as predicted.

42 primase fragment catalyzes the synthesis of oligoribonucleotides at rates similar to those catalyzed

43 oate oligonucleotides containing 2'-O-methyl oligoribonucleotides at the 3' and 5' termini.

44 cture reveals that the single-stranded hepta-oligoribonucleotide binds in a circular conformation aro

45 y the primase fragment at the 5'-ends of the oligoribonucleotides but not at the 3'-ends.

46 termined the structure of the 19-residue SL2 oligoribonucleotide by heteronuclear NMR methods.

47 mechanism involves the production of 4-8 nt oligoribonucleotides by abortive synthesis by the viral

48 eIF4E (eIF4E(P)) with cap analogs and capped oligoribonucleotides by stopped-flow kinetics.

49 peptides slow the annealing of complementary oligoribonucleotides by up to several thousand-fold; how

50 hat contain synthetic genes coding for small oligoribonucleotides called external guide sequences (EG

51 Because the cleavage of the synthetic oligoribonucleotide can be used to monitor the steady-st

52 gment ions in both the unlabeled and labeled oligoribonucleotides can be used to gain further confide

53 n of prepro-nociceptin are identified by an 'oligoribonucleotide-capping' method, but the major one i

54 nd potential improvements in the efficacy of oligoribonucleotides, chemical modification may also pro

55 DNA synthesis was initiated from (i) an oligoribonucleotide complementary to the primer-binding

56 re it is shown that binding of a 2'-O-methyl-oligoribonucleotide complementary to U5 small nuclear RN

57 Antisense 2'-O-methyl oligoribonucleotides complementary to selected SF2/ASF b

58 d the crystal structure of an Sa Hfq-adenine oligoribonucleotide complex.

59 between EF-G and a sarcin/ricin domain (SRD) oligoribonucleotide containing 5-iodouridine.

60 G, but it cannot catalyze the cleavage of an oligoribonucleotide containing only cytosines.

61 A biotinylated oligoribonucleotide containing the direct repeat was use

62 tability is comparable to that observed with oligoribonucleotides containing 2'-O-methylated residues

63 Oligoribonucleotides containing 3'-S-phosphorothiolate l

64 To facilitate the synthesis of oligoribonucleotides containing 4-thiouridine, we have d

65 hosphoramidites for solid-phase synthesis of oligoribonucleotides containing a 2'-O-photocaged 5'-S-p

66 Oligoribonucleotides containing a 5'-phosphorothiolate l

67 cribes a general method for the synthesis of oligoribonucleotides containing a site-specific nonbridg

68 A series of oligoribonucleotides containing different 3 x 3 internal

69 endent RNA polymerase that synthesizes short oligoribonucleotides containing each of the four canonic

70 cal melting experiments, are reported for 28 oligoribonucleotides containing frequently occurring sin

71 Optical melting experiments on oligoribonucleotides containing internal loops of three

72 By using synthetic oligoribonucleotides containing repeats of identical tar

73 ting studies are reported for a series of 14 oligoribonucleotides containing single I x U pairs adjac

74 o determine the thermodynamic parameters for oligoribonucleotides containing small asymmetric interna

75 105-115 of the 5'-UTR (U5) readily binds to oligoribonucleotides containing the gag start codon (AUG

76 Using a 17 residue oligoribonucleotide corresponding to the anticodon arm o

77 A 17-base oligoribonucleotide corresponding to the anticodon stem-

78 rected mutants using DMAPP and a 17-base RNA oligoribonucleotide corresponding to the stem-loop regio

79 ynthetic pre-miRNAs and even single-stranded oligoribonucleotides corresponding to miRNA loops, we re

80 ligodeoxyribonucleotide (MPO) and its target oligoribonucleotide, d(T(MP)CC(MP)-TT(MP)AG(MP)CT(MP)CC(

81 Extracellularly delivered ssRNA40, an oligoribonucleotide derived from HIV and an established

82 preparation of branched and branched cyclic oligoribonucleotides derived from adenosine.

83 tic, template-directed ligation reactions of oligoribonucleotides display high selectivity for the fo

84 e derived from optical melting studies of 90 oligoribonucleotide duplexes containing only Watson-Cric

85 Oligoribonucleotide duplexes containing small internal l

86 Furthermore, oligoribonucleotide duplexes with parallel strand orient

87 chemical stability of these highly activated oligoribonucleotides during synthesis and long-term stor

88 gonucleotides were prehybridized with 17-mer oligoribonucleotides, extracts prepared from T24 cells,

89 DNA primases provide oligoribonucleotides for DNA polymerase to initiate lagg

90 The primase catalyzes the synthesis of oligoribonucleotides for the initiation of lagging stran

91 TTP, and catalyze the synthesis of short RNA oligoribonucleotides for use as primers by T7 DNA polyme

92 nder them superior to corresponding 2'-deoxy oligoribonucleotides for use in assays that detect RNA t

93 yme, Dcs1, catalyses cleavage of 5'end m(7)G-oligoribonucleotide fragments generated by 3'-->5' exonu

94 Reduction in Ha-Ras RNA was dependent on the oligoribonucleotide gap size with the minimum gap size b

95 osol, and nuclei resulted in cleavage in the oligoribonucleotide gap.

96 thoxy 5' and 3' "wings" on either side of an oligoribonucleotide gap.

97 Synthesis of partially 2'/3'-O-acetylated oligoribonucleotides has been accomplished by using a 2'

98 petitions in Xenopus oocytes with 2'O-methyl oligoribonucleotides have confirmed this region as a fun

99 Incorporation of 4'-thiocytidines into oligoribonucleotides improved the thermal stability of t

100 A random coil 17-base oligoribonucleotide in which the loop sequence of E. col

101 ective than the phosphorothioate 2'-O-methyl-oligoribonucleotides in free uptake from the culture med

102 T7 gene 4 protein catalyzes the synthesis of oligoribonucleotides in the presence of ATP, CTP, Mg(2+)

103 purified recombinant D5 protein synthesized oligoribonucleotides in vitro.

104 sis of the effect of alpha-sacrin on variant oligoribonucleotides in which additional bases were inse

105 tes the rapid and accurate quantification of oligoribonucleotides, including cyclic phosphate interme

106 Our studies show that the 5' GG of the bound oligoribonucleotide interacts extensively with highly co

107 s a demonstration, invasion of a 2'-O-methyl oligoribonucleotide into an RNA hairpin model (HIV-1 TAR

108 trate that injection of 2'O-methyl antisense oligoribonucleotides into early Drosophila embryos leads

109 that can be used to address anti-replicative oligoribonucleotides into human mitochondria and thus im

110 dicate that the 5'-most cleavage site on the oligoribonucleotide is positioned 7 bp from the first 3'

111 ound to ribosomes and the analog of G2655 in oligoribonucleotides is critical for recognition by the

112 thesis and its subsequent incorporation into oligoribonucleotides is described.

113 ase are crucial for the stabilization of the oligoribonucleotide, its transfer to the polymerase, and

114 nt of an efficient chemical strategy to make oligoribonucleotide-ligand conjugates using the copper-c

115 f pyrophosphate-activated, template-directed oligoribonucleotide ligation has been investigated.

116 An increase in the stability of the mutant oligoribonucleotides may be the basis of the impairment

117 eterohexamers synthesize a reduced amount of oligoribonucleotides, mediated predominately by the 63-k

118 thin the intron substrates (guanosine and an oligoribonucleotide mimic of the 5'-exon) that coordinat

119 Short synthetic oligoribonucleotide mimics of the RNA-1 TABS and the RNA

120 rization of RNA mimetics, uniformly modified oligoribonucleotide N3'-->P5' phosphoramidates containin

121 The properties of the oligoribonucleotide N3'-->P5' phosphoramidates indicate

122 thesis and properties of novel RNA mimetics, oligoribonucleotide N3'-->P5' phosphoramidates, are desc

123 ce for binding to poly(A) RNA relative to an oligoribonucleotide of the same length and a random sequ

124 apid purification and structural analysis of oligoribonucleotides of 19 and 20 nt is applied to RNA h

125 Titration experiments with oligoribonucleotides of varying lengths and compositions

126 onucleotide analogues, namely, a 2'-O-methyl oligoribonucleotide (OMe), a chimeric oligonucleotide co

127 imase of phage T7 catalyzes the synthesis of oligoribonucleotides on single-stranded DNA templates.

128 tivity, catalysing the synthesis of unprimed oligoribonucleotides on single-stranded DNA templates.

129 otides, but T7 primase can synthesize longer oligoribonucleotides on templates containing long stretc

130 Recombinant Mjpri is able to synthesise oligoribonucleotides on various pyrimidine single-strand

131 identifies a novel class of single-stranded oligoribonucleotides (ORN) containing unmethylated CpG m

132 art from foreign and host RNA, synthetic RNA oligoribonucleotides (ORN) or small molecules of the imi

133 bit TS expression, antisense 2'-O-methyl RNA oligoribonucleotides (ORNs) were designed to directly ta

134 by short, nuclease-resistant, non-extendable oligoribonucleotides (ORNs).

135 R-17-92 knockdown with antisense 2'-O-methyl oligoribonucleotides partly restored Tsp1 and CTGF expre

136 Several mixed base 9-13mer oligoribonucleotide phosphoramidates were synthesized wi

137 ge T7 primase catalyzes the synthesis of the oligoribonucleotides pppACC(C/A) and pppACAC from the si

138 igated whether lexaptepid, an antihepcidin l-oligoribonucleotide, prevents the decrease in serum iron

139 mediated extension of abortively synthesized oligoribonucleotide primers complementary to the 3' end

140 Synthesis of oligoribonucleotide primers for lagging-strand DNA synth

141 te-dependent RNA polymerases that synthesize oligoribonucleotide primers that can be extended by DNA

142 d (ss) DNAs as templates to synthesize short oligoribonucleotide primers that initiate lagging strand

143 nt RNA polymerases have been reported to use oligoribonucleotide primers to initiate nucleic acid syn

144 n of rRNA whereas the same 17 base 2'- deoxy oligoribonucleotide probe did not.

145 A 12 base 2'-O-methyl oligoribonucleotide probe had the same Tm as a 19 base 2

146 A 17 base 2'-O-methyl oligoribonucleotide probe was able to bind a double-stra

147 probe had the same Tm as a 19 base 2'-deoxy oligoribonucleotide probe when bound to a matched RNA ta

148 much larger decrease in Tm than the 2'-deoxy oligoribonucleotide probe when bound to an RNA target co

149 ures (Tm values) than corresponding 2'-deoxy oligoribonucleotide probes at all lengths tested (8-26 b

150 In contrast to RNA targets, 2'-O-methyl oligoribonucleotide probes bound more slowly and with th

151 2'-O-Methyl oligoribonucleotide probes bound to RNA targets faster a

152 en bound to RNA targets, shorter 2'-O-methyl oligoribonucleotide probes can be used in assays in plac

153 y enhanced Tm when bound to RNA, 2'-O-methyl oligoribonucleotide probes can efficiently bind to doubl

154 ed various kinetic and melting properties of oligoribonucleotide probes containing 2'-O-methylnucleot

155 ences in Tm between 2'-O-methyl and 2'-deoxy oligoribonucleotide probes observed at lengths of 16 bas

156 ets and increased specificity of 2'-O-methyl oligoribonucleotide probes render them superior to corre

157 e used in assays in place of longer 2'-deoxy oligoribonucleotide probes, resulting in enhanced discri

158 Tm to DNA targets as corresponding 2'-deoxy oligoribonucleotide probes.

159 a single-step deprotection of the resulting oligoribonucleotide product using 1,2-diamines under anh

160 fraction of the resulting products is small oligoribonucleotides rather than the mononucleotides gen

161 neral semisynthetic strategy to obtain these oligoribonucleotides reliably and relatively efficiently

162 l proteins (nsPs) and a (32)P-labeled 24-mer oligoribonucleotide representing the minimal sequence wi

163 tro and in vivo, and inclusion of competitor oligoribonucleotides representing the USEs specifically

164 DNA primases catalyze the synthesis of oligoribonucleotides required for the initiation of lagg

165 DNA primases catalyze the synthesis of the oligoribonucleotides required for the initiation of lagg

166 introducing a synthetic 2'-O-methyl tRF-1001 oligoribonucleotide resistant to the siRNA.

167 udies with RNA homopolymers and a variety of oligoribonucleotides revealed that RNase T displays an u

168 and have a modified oligodeoxynucleotide or oligoribonucleotide segment located in the central porti

169 The corresponding oligoribonucleotide sequences (5' rGCGAAUUCGC)2, (5' rGC

170 These methods produce branched oligoribonucleotide sequences of arbitrary length, base

171 This means that phosphorothioate-containing oligoribonucleotides should also be useful for mapping p

172 The rate constant for association of the oligoribonucleotide substrate (S) increased 12-fold from

173 No base specificity was observed with an oligoribonucleotide substrate.

174 e recognition of AREs by AUF1 in vitro using oligoribonucleotide substrates.

175 y, indicates amino acids likely critical for oligoribonucleotide synthesis as well as a putative Cys(

176 However, oligoribonucleotide synthesis by SP6 primase is not stim

177 T7 DNA primase to catalyze template-directed oligoribonucleotide synthesis is eliminated by substitut

178 keeping with this property, Mcm10p supported oligoribonucleotide synthesis of short RNA primers (pref

179 itate DNA binding, leading to more efficient oligoribonucleotide synthesis on short templates.

180 to the 5'-end have no effect on the rate of oligoribonucleotide synthesis or the affinity of the pri

181 ing d2APy in place of the cryptic dC support oligoribonucleotide synthesis whereas those containing 3

182 oxyuridine (dU) substitutions for dT support oligoribonucleotide synthesis whereas those containing 5

183 a primase recognition site does not inhibit oligoribonucleotide synthesis, suggesting that the prima

184 2'-deoxyinosine (dI) in place of dG support oligoribonucleotide synthesis.

185 CTP are both required for the initiation of oligoribonucleotide synthesis.

186 are required for tight DNA binding and rapid oligoribonucleotide synthesis.

187 , in part, responsible for template-directed oligoribonucleotide synthesis.

188 conformation leads to loss of DNA-dependent oligoribonucleotide synthesis.

189 domain is responsible for template-directed oligoribonucleotide synthesis.

190 The T7 DNA polymerase can use oligoribonucleotides synthesized by SP6 primase as prime

191 he lagging-strand DNA polymerase requires an oligoribonucleotide, synthesized by DNA primase, to init

192 e incorporated into an antisense 2'-O-methyl oligoribonucleotide targeted to the 3' region of the PKC

193 r, treatment of the cells with a 2'-O-methyl-oligoribonucleotide targeted to the aberrant splice site

194 ngle-stranded antisense 2'-O-methyl (2'-OMe) oligoribonucleotides targeting microRNA (miRNA).

195 ti-hepcidin compound NOX-H94, a structured L-oligoribonucleotide that binds human hepcidin with high

196 nds YS11 with a Kd value of 230 nM; a second oligoribonucleotide that contains only the kink-turn mot

197 In the presence of a specific competitor oligoribonucleotide that inhibits MnSOD RNA protein-bind

198 An oligoribonucleotide that mimics helix 11, a phylogenetic

199 317 and C4331, and to U4316 and C4332, in an oligoribonucleotide that mimics the sarcin/ricin domain

200 The oligoribonucleotides that accumulate are 2-5 residues in

201 ase L can catalyze the cleavage of synthetic oligoribonucleotides that contain dyad sequences of the

202 ve studied its interactions with a series of oligoribonucleotides that contain one or more of the Psi

203 A series of oligoribonucleotides that form purine-rich internal loop

204 mal protein, in a filter retention assay, to oligoribonucleotides that reproduce regions of 18 S rRNA

205 t a replication fork DNA primase synthesizes oligoribonucleotides that serve as primers for the laggi

206 Deletion from oligoribonucleotides, that reproduce the sarcin/ricin do

207 Although it is not copied into the oligoribonucleotides, the cytosine at the 3'-position is

208 8 and G2663 decreased binding of EF-G to SRD oligoribonucleotides; the same mutations in 23 S rRNA de

209 teractions that occur during delivery of the oligoribonucleotide to DNA polymerase, we have used four

210 sense sequence hybridized to a complementary oligoribonucleotide to evaluate both the binding affinit

211 nvolves use of RNA ligase to link a specific oligoribonucleotide to the 5' ends of cellular RNAs, fol

212 re conducted with chemically synthesized RNA oligoribonucleotides to determine the essential elements

213 DNA primases catalyze the synthesis of oligoribonucleotides to initiate lagging strand DNA synt

214 on that does not require base pairing of the oligoribonucleotides to the mutant, positive-strand RNA

215 clear magnetic resonance of synthetic G-rich oligoribonucleotide tracts derived from this region show

216 eak ends and a phosphoesterase that trims 3'-oligoribonucleotide tracts until only a single 3'-ribonu

217 rhead ribozymes were synthesized from linear oligoribonucleotides using T4 RNA ligase.

218 RNA contact was not detected after a labeled oligoribonucleotide was released from the complex by nas

219 We found that the 27-base HIPBS oligoribonucleotide was sufficient to bind the protein i

220 3'-to-5' exoribonuclease specific for small oligoribonucleotides, was purified to homogeneity from e

221 ucleotides (dangling ends) at the termini of oligoribonucleotide Watson-Crick helixes (DeltaG(0)37,st

222 veral deletion RNA derivatives and synthetic oligoribonucleotides were constructed from the S segment

223 Thiouridine-containing oligoribonucleotides were used as 350 nm UV crosslinking

224 Three oligoribonucleotides were used to measure binding affini

225 The initiation site binds ATP or oligoribonucleotides, whereas the elongation site binds

226 Using a short oligoribonucleotide which contains all the necessary seq

227 The binding of EF-G to oligoribonucleotides with a U2653/C2667 double deletion

228 MicroRNAs (miRNAs) are endogenous oligoribonucleotides with exciting therapeutic potential

229 hat allows high-yield automated synthesis of oligoribonucleotides with pyrene incorporated at a speci

230 h ribozyme and substrate being two different oligoribonucleotides with regions of complementarity.

231 cleavage resynthesis steps, producing short oligoribonucleotides with uridine residues at the 3' ter