ライフサイエンスコーパス: optical trap

1 out influences from optical gradient forces (optical trapping).

2 sitioned near microtubule plus ends using an optical trap.

3 es under the low-force regime afforded by an optical trap.

4 es them well-suited for manipulation with an optical trap.

5 from the microtubule under the force of the optical trap.

6 ep size under applied load as measured in an optical trap.

7 nating hindering and assisting loads with an optical trap.

8 an elastic lift force upon release from the optical trap.

9 evels when external tension is applied by an optical trap.

10 ment kinetics with varying tensions using an optical trap.

11 rque application and detection in an angular optical trap.

12 .6 degrees C/100 mW in a nonheating (830 nm) optical trap.

13 ulating the input polarization in a standard optical trap.

14 eosomes in a single chromatin fiber using an optical trap.

15 A-binding proteins using a feedback-enhanced optical trap.

16 nucleosomal arrays with a feedback-enhanced optical trap.

17 ove effectively against a load imposed by an optical trap.

18 forces were applied by a feedback-controlled optical trap.

19 ct-induced phase changes being studied in an optical trap.

20 lity but increased force production using an optical trap.

21 he stroke size, as observed with a dual-beam optical trap.

22 en DNA handles that are attached to beads in optical traps.

23 he loading efficiency of (209,210)Fr magneto-optical traps.

24 armonic potential trap analogous to atoms in optical traps.

25 sing arrays of time-multiplexed, holographic optical traps.

26 at both ends by microspheres in two separate optical traps.

27 tanium-sapphire (700-990 nm) laser microbeam optical traps.

28 ached to two beads that were held in the two optical traps.

29 ing dual-labeled gliding filament assays and optical trapping.

30 apillary electrophoresis, patch-clamping and optical trapping.

31 in aqueous solution in a manner analogous to optical trapping.

32 l structure of molecules complicates magneto-optical trapping.

33 ing with 1-nm accuracy (FIONA) and dual-beam optical trapping.

34 , as revealed by atomic force microscopy and optical trapping.

35 oughout the near-infrared region favored for optical trapping (790-1064 nm).

36 ivated by attaching the motor to beads in an optical trap, a situation that may mimic attachment to I

37 With the use of optical trapping, a single vesicle that had attoliters (

38 Single-molecule optical trapping allows ClpXP unfolding to be directly v

39 aman scattering from a single vesicle, while optical trapping allows more than hour-long observations

40 Optical trapping allows noninvasive probing of piconewto

41 x-ray scattering (SAXS) and single-molecule optical-trap analyses are consistent with the high bendi

42 Using an optical trap and a two-channel flow cell to move single

43 n progressively through the transition by an optical trap and an algorithm is used to extract the ene

44 force required to hold the bacterium in the optical trap and determine the propulsion matrix, which

45 e this problem by alternately modulating the optical trap and excitation beams to prevent simultaneou

46 membranes of various cell types by using an optical trap and fast three-dimensional (3D) interferome

47 upper DPPC bilayer can be manipulated by the optical trap and the shape of the vesicle distorted from

48 r aqueous aerosol droplet is captured in the optical trap and used as a sampling volume, accreting ma

49 nts of various lengths using single-molecule optical trapping and bulk fluorescence approaches in the

50 g cells, thereby extending cell viability in optical trapping and cell manipulation applications.

51 Using an in vitro optical trapping and fluorescence assay, we found that K

52 Optical trapping and levitation allow a particle to be m

53 Optical trapping and levitation also maintain optical al

54 t the single particle level using near-field optical trapping and light-scattering techniques.

55 Noncontact optical trapping and manipulation of micrometer- and nan

56 The techniques of optical trapping and manipulation of neutral particles b

57 his paper describes a method, which combines optical trapping and microfluidic-based droplet generati

58 hat are arranged by electrodynamic (that is, optical trapping and optical binding) interactions.

59 ucidates the role of convection in plasmonic optical trapping and particle assembly, and opens up new

60 Here we demonstrate simultaneous optical trapping and rotation of a birefringent micropar

61 apable of simultaneous, spatially coincident optical trapping and single-molecule fluorescence.

62 We use single-molecule optical trapping and small-angle x-ray scattering, combi

63 We combine optical trapping and surface-enhanced Raman scattering t

64 We show using single molecule optical trapping and transient kinetics that the unusual

65 integrin-molecule adhesion kinetics using an optical trap, and diffusion using fluorescence correlati

66 of the diffusion coefficient of a bead in an optical trap, and to demonstrate that it is not in gener

67 Here, we employ an optical-trap- and total internal reflection fluorescence

68 An ultrastable optical trapping apparatus capable of base pair resoluti

69 Here, we used an automated optical trapping apparatus in conjunction with a novel m

70 transcription elongation complexes, using an optical trapping apparatus that allows for the detection

71 Here, by combining ultrastable optical trapping apparatus with a novel two-bead assay t

72 Using a custom-modified optical trapping apparatus, we used a tightly focused in

73 Fermionic lithium-6 atoms in an optical trap are evaporatively cooled to degeneracy usin

74 chniques currently available, those based on optical trapping are promising.

75 The tight focus excitation requirements of optical trapping are well suited to confocal Raman micro

76 been an experimental challenge, as standard optical traps are too weak.

77 f tension applied with a feedback-controlled optical trap as the MTs lengthened approximately 1 micro

78 A molecules have previously been observed in optical traps as sudden changes in molecular extension.

79 rm the reader about recent progress in axial optical trapping, as well as the potential for these dev

80 Using a single-molecule optical trap assay, we found that vinculin forms a force

81 ion, applied load, and temperature, using an optical trapping assay capable of distinguishing pauses

82 We employ an optical trapping assay to investigate the behaviors of t

83 We employed an optical trapping assay to probe the motions of individua

84 ium of a DNA hairpin using a single-molecule optical trapping assay, in which the unfolded state is c

85 Using a three-bead optical trapping assay, we recorded NMIIB interactions w

86 With the use of an optical-trapping assay based on in situ transcription by

87 sphate (ATP) (2-4 muM) was measured using an optical-trapping assay featuring 1 base-pair (bp) precis

88 We developed an optical-trapping assay to follow the cotranscriptional f

89 We employed an ultrastable optical-trapping assay to follow the motion of individua

90 Here, we developed an optical-trapping assay to monitor the translocation of i

91 Here, we describe a single-molecule optical-trapping assay to study transcription initiation

92 Here, we used an optical-trapping assay with high spatiotemporal resoluti

93 recision to the widely used, surface-coupled optical-trapping assay.

94 the bead rotational fluctuations inherent in optical trapping assays where beads are used to apply th

95 The most commonly used optical-trapping assays are coupled to surfaces, yet suc

96 Such enhanced optical-trapping assays are revealing the fundamental st

97 Using an optical trap-based assay, we showed that the minimal cad

98 O) at the single-molecule level, we utilized optical trap-based force spectroscopy to measure the str

99 Here we used an optical trap-based system to measure the binding of sing

100 nally constrained DNA when measured using an optical-trap-based DNA-overstretching assay.

101 Employing an optical-trap-based electronic force clamp, we studied th

102 ity of myosin to produce force using a novel optical-trap-based isometric force in vitro motility ass

103 es, labeling with beads remains critical for optical-trapping-based investigations of molecular motor

104 ich are highly sought after in the fields of optical trapping, biological sensing and quantum informa

105 ing in the well-controlled environment of an optical trap but also for spores germinating when adhere

106 s from such cells by pulling on them with an optical trap but failed, even when we used forces large

107 eeds of the spheres were monitored in a weak optical trap by back-focal-plane interferometry in solut

108 ns of (7)Li atoms in a quasi-one-dimensional optical trap, by magnetically tuning the interactions in

109 The flow and the optical trap can be used to exert forces on the beads, t

110 Here we show that an infrared single-beam optical trap can be used to individually trap, transfer

111 We show that optical trapping can efficiently deform cell-cell interf

112 rpin, recorded under constant force using an optical trap, can be used to reconstruct the energy land

113 was constructed, based on a feedback-driven optical trap capable of maintaining constant loads on si

114 A single-beam optical trap capable of trapping micron-scale polystyren

115 analyzed individually with a combination of optical trapping, capillary electrophoresis separation,

116 We found that, on manipulation with an optical trap, cilia deflect by bending along their lengt

117 We demonstrate that optical trapping combined with confocal Raman spectrosco

118 eriments on actin filaments manipulated with optical traps confirm the scaling law predicted by the a

119 Optical-trapping confocal Raman microscopy is developed

120 ly 0.6 microm in size) have been acquired by optical-trapping confocal Raman microscopy over the 900-

121 work with laser tweezers has suggested that optical traps could be used to create novel spatial prob

122 we develop an in silico model, supported by optical trapping data, suggesting that the motors' diffu

123 Here, we report a new class of on-chip optical trapping devices.

124 sed on scanning force microscopy imaging and optical trap-driven unzipping assays, it has recently be

125 at probe the H-NS-DNA interaction: a dynamic optical-trap-driven unzipping assay and an equilibrium H

126 When moving against load applied by an optical trap, dynein can decrease step size to 8 nm and

127 Calcium imaging combined with an optical trap enabled the T cell contact requirements and

128 The optical trapping enables capturing of individual bacteri

129 s interaction with the anisotropic fluid and optical trap environment.

130 orough characterization of cell viability in optical trapping environments was performed.

131 resonance energy transfer (smFRET), using an optical trap equipped for simultaneous smFRET.

132 Recent optical trap experiments have applied resisting, assisti

133 Optical trapping experiments indicate that dynein bound

134 Optical trapping experiments reveal details of molecular

135 to perform high-precision and high-bandwidth optical trapping experiments to study motor regulation i

136 Optical trapping experiments were used to measure direct

137 ) = 6.2 s(-1)) determined in single molecule optical trapping experiments, indicating that myosin 15-

138 Yet, as in previous optical trapping experiments, the forces imposed on carg

139 In optical trapping experiments, we found that increasing t

140 essive by using single molecule motility and optical trapping experiments.

141 Single-molecule optical-trapping experiments are now resolving the small

142 gyroscopic directional stabilization and the optical trapping field.

143 Using an optical-trap/flow-control video microscopy technique, we

144 Using an optical trap for spatial and temporal control over targe

145 Using optical trap force spectroscopy, we investigated the res

146 hered microsphere from equilibrium using the optical trapping force, the tensions of individual stran

147 d concentrations and different directions of optical trap forces.

148 The nuclei of cells were exposed to optical trapping forces at various wavelengths, power de

149 ams in these fluids can generate anisotropic optical trapping forces, even for particles larger than

150 rties, and the organelles manipulated by the optical trap frequently vary in size and shape.

151 Here by using a DNA-tethered optical trapping geometry, we find that the force-genera

152 tal advances are complemented by insights in optical-trapping geometry and single-molecule motility a

153 3 muK in a micro-fabricated grating magneto-optical trap (GMOT), enabling future mass-deployment in

154 While optical trapping has been the most explored method of le

155 The application of optical traps has come to the fore in the last three dec

156 Optical tweezers (infrared laser-based optical traps) have emerged as a powerful tool in molecu

157 pplying a slow triangle-wave movement to the optical traps holding a bead-actin-bead dumbbell.

158 e bio-chemical detection, reflective filter, optical trapping, hot-electron generation, and heat-assi

159 Optical trapping immobilizes the particle while maintain

160 hods that provide in situ calibration of the optical trap in the complex cellular environment, taking

161 Here we employed a wavelength-tunable optical trap in which the microscope objective transmiss

162 We describe the exciting advances of using optical trapping in the field of analytical biotechnolog

163 hough our magnetic moulds currently resemble optical traps in that they are limited to the manipulati

164 molecular oxygen is generated locally by the optical traps in the presence of a sensitizer, which we

165 al calibrations, results in a more versatile optical trapping instrument that is accurately calibrate

166 We constructed a next-generation optical trapping instrument to study the motility of sin

167 Using a high-resolution optical trapping instrument, we directly observed the pr

168 Miniaturizing optical trap instruments onto optofluidic platforms hold

169 ipulation of live cells in a dual-beam fibre-optical trap integrated into a modular lab-on-chip syste

170 ines a time-shared ultrahigh-resolution dual optical trap interlaced with a confocal fluorescence mic

171 to apply forces on single molecules with an optical trap is combined with the endogenous structural

172 A type of dark-spot optical trap is devised that can cool large numbers of a

173 Optical trapping is a powerful manipulation and measurem

174 Plasmon-enhanced optical trapping is being actively studied to provide ef

175 Optical trapping is potentially a powerful technique in

176 e of processive myosin motors as measured by optical trapping is similarly uncertain.

177 ended between two beads and held in separate optical traps is brought close to a surface that is spar

178 n array of stable, three-dimensional on-chip optical traps is formed at the antinodes of a standing-w

179 At low optical trap loads, we observed staircase-like processiv

180 These results indicate that typical optical trap measurements of kinetics reflect the dynami

181 This method was applied to optical trap measurements of power strokes of the Drosop

182 Optical trap measurements revealed that the heterodimer

183 We propose and demonstrate a new optical trapping method for single cells that utilizes m

184 Using a modified optical-trapping method, we examined the group function

185 We report on optical trapping methodology capable of making precise i

186 in molecules in real time using magnetic and optical trapping micromanipulation techniques.

187 assay in conjunction with a high-resolution optical trapping microscope, we have examined the behavi

188 In this study, a high-resolution optical-trap microscope was used to measure directly the

189 echnique in cold-atom physics is the magneto-optical trap (MOT), which combines laser cooling with a

190 from the vapour can be captured in a magneto-optical trap (MOT).

191 We tested this proposal by comparing optical trapping motility measurements of cover strand m

192 Here, we use optical-trapping nanometry to probe the mechanics of enz

193 ere we demonstrate three-dimensional magneto-optical trapping of a diatomic molecule, strontium monof

194 The energy and forces involved in optical trapping of lipid vesicles were derived in terms

195 Optical trapping of liposomes is a useful tool for manip

196 Successful optical trapping of magnetic beads was found to be depen

197 Magneto-optical trapping of molecules could provide a similarly

198 The results are consistent with the optical trapping of particles at or near the excitation

199 arming motility assays, video microscopy and optical trapping of single cells.

200 Optical trapping of single molecules in three-bead assay

201 Optical trapping of small structures is a powerful tool

202 Detection involves the optical trapping of solitary, fluorescently tagged dsDNA

203 The next generation of single-beam optical traps offers revolutionary new opportunities for

204 nti-integrin antibody was restrained with an optical trap on fibroblasts to mimic extracellular attac

205 ic device may find potential applications in optical trapping, optical data storage and many other re

206 tem, were immobilized with an infrared laser optical trap or by adhesion to modified borosilicate gla

207 Optical traps or "tweezers" use high-power, near-infrare

208 mic-force microscopy, magnetic tweezers, and optical traps (OTs) have been employed to probe the many

209 ic field enhancement in the plasmon-assisted optical trapping process.

210 xciton confinement layer, and a conventional optical trapping scheme, we show a peak external quantum

211 plets for high-resolution measurements in an optical trap showed that they compare well with plastic

212 Single-molecule analysis of myosin by optical trapping showed a comparable 2-fold reduction in

213 The model reproduces key signatures found in optical trapping studies of structurally defined complex

214 s work, we improved the spatial precision of optical trapping studies of transcription to approximate

215 e probability of multiple-motor transport in optical trapping studies.

216 A new optical trapping study shows that the stepsize of cytopl

217 We present a single-molecule optical-trapping study of the interactions of RNAPII wit

218 Using a dual optical trap system, we observed tether formation betwee

219 e present the development of an ultra-stable optical trapping system with angstrom-level resolution,

220 accuracy is crucial for force calibration of optical trapping systems.

221 In this study, we established the optical trapping technique for determining membrane mech

222 A novel optical trapping technique is described that combines an

223 Here, we use our recently developed optical trapping technique to characterize the swimming

224 e under tension and torque using the angular optical trapping technique.

225 Here we employ optical trapping techniques to investigate the structure

226 anscription elongation using single-molecule optical trapping techniques.

227 Using single-molecule optical-trapping techniques, we examined the force-induc

228 Using single molecule optical-trapping techniques, we found that betaCM has a

229 We built an optical trap that can be calibrated in vivo during data

230 g pathways under the low-force regime of the optical trap; the specific unfolding pathway depends on

231 to freely control the number and position of optical traps, thus facilitating the unrestricted manipu

232 old-coated liposomes are maneuvered using an optical trap to achieve precise delivery of encapsulated

233 The ability of the optical trap to alter either the structure or biochemist

234 We used an optical trap to deflect bullfrog hair bundles and to mea

235 use measurements of swimming bacteria in an optical trap to determine fundamental properties of bact

236 We have used an optical trap to directly measure the forces generated by

237 We used an optical trap to directly probe the molecular determinant

238 idual DNA molecules were manipulated with an optical trap to examine the kinetics of torus formation

239 We have used a feedback-enhanced optical trap to examine the stepping kinetics of this mo

240 l properties of a primary cilium by using an optical trap to induce resonant oscillation of the struc

241 We further use the optical trap to measure in vivo the detachment rates fro

242 Here, we used an optical trap to measure the bending rigidity of live Esc

243 We utilized an optical trap to measure the mechanical force to rupture

244 stigate these questions, we used a precision optical trap to measure the single-molecule kinetics of

245 The methodology uses the optical trap to probe force-free association of individu

246 We used a feedback-controlled optical trap to probe the velocity, run length, and unbi

247 Here, we use an optical trap to quantify motion of polystyrene beads dri

248 Here, we use single-molecule optical trapping to determine the mechanochemistry of tw

249 Here, we used optical trapping to determine the unitary and ensemble f

250 ngle-molecule gold nanoparticle tracking and optical trapping to examine the mechanism of coordinatio

251 ce (flow cell) used in conjunction with dual optical trapping to manipulate DNA dumbbells and to visu

252 Using dual optical trapping to manipulate DNA, and single-molecule

253 erties of microtubule cross-links we applied optical trapping to mitotic asters that form in mammalia

254 d confocal Raman microscope is combined with optical trapping to probe and analyze bacterial spores i

255 We used high-resolution optical trapping to study individual RecBCD molecules mo

256 w vancomycin affects cell stiffness, we used optical traps to bend unflagellated mutants of B. burgdo

257 et al., who use latex spheres manipulated by optical traps to pump fluids.

258 parameters must be considered when applying optical traps to the study of biological problems, espec

259 Using an optical-trap transducer, we have measured the unitary di

260 hoice of wavelength and polarization for the optical trap, two electronic states of an atom can exper

261 cal and ensemble-level experiments involving optical trapping using a photonic force microscope and p

262 ith a fluorophore at high efficiency, and 3) optical trapping virometry to measure the number of gp12

263 A two-beam optical trap was used to measure the bending stiffness o

264 (CHO) cells was conducted after exposure to optical trapping wavelengths using Nd:YAG (1064 nm) and

265 ally, by measuring the cargo dynamics in the optical trap, we find that there is memory: it is more l

266 ules held under tension in a high-resolution optical trap, we found that the native folding pathway i

267 of nitrogen-vacancy centers suspended in the optical trap, we observe distinct peaks in the measured

268 With the optical trap, we stretched VWF multimers and a poly-prot

269 Using an improved application of an optical trap, we were now able to demonstrate that cytop

270 Using optical trapping, we observed myosin VI stepping against

271 ther, by integrating these enhancements with optical traps, we demonstrate how efficient bioconjugati

272 Using optical traps, we determine physicochemical triggering t

273 toms confined in an array of two-dimensional optical traps; we studied the spin-orbital quantum dynam

274 d motions in the focal plane relative to the optical trap were detected by measuring laser intensity

275 ingle living cells and beads suspended in an optical trap were recorded with 30-ms time resolution.

276 A classic example of this is an optical trap, which can hold a particle in a tightly foc

277 an one IgG per contact area were held in the optical trap while an SpA-coated substrate was scanned b

278 living microalgal cell held in place with an optical trap while simultaneously collecting Raman data.

279 e living bacterial cells held in place by an optical trap while simultaneously collecting Raman spect

280 d droplets (LDs) in COS1 cells respond to an optical trap with a remarkable enhancement in sustained

281 low laser power by combining a standing-wave optical trap with confocal Raman spectroscopy.

282 n of the biopolymer's elasticity by using an optical trap with nanometer-scale position resolution.

283 Combined optical trapping with single-molecule fluorescence imagi

284 Here, we combine optical trapping with TIRF-based microscopy to measure t

285 Finally, we demonstrate an array of magneto-optical traps with a single laser beam, which will be ut

286 we show that the spatial resolution of dual optical traps with dual-trap detection is always superio

287 usand times more atoms than previous magneto-optical traps with microfabricated optics and, for the f

288 ayer from the same vesicle is drawn into the optical trap, with an energy of approximately 6 x 10(-13

289 By setting up a well-calibrated single-beam optical trap within a fluorescence microscope system, on

290 s and experimental protocols best suited for optical trapping work.