ライフサイエンスコーパス: optical tweezer

1 h mediation by dynamic holographic assembly (optical tweezers).

2 d beads suspended in water and acetone by an optical tweezer.

3 orce microscope probe coupled to a plasmonic optical tweezer.

4 facile fusion with another droplet using an optical tweezer.

5 quent initiation processes in real time with optical tweezers.

6 characterize its mechanical properties with optical tweezers.

7 le DNA molecules in bacteriophage phi29 with optical tweezers.

8 tructure, which is monitored in real time by optical tweezers.

9 two laser-cooled (87)Rb atoms trapped in two optical tweezers.

10 and single-molecule force spectroscopy with optical tweezers.

11 single-molecule level using high-resolution optical tweezers.

12 y developed to apply and measure force using optical tweezers.

13 ere measured at the single-molecule level by optical tweezers.

14 re in line with previous studies that employ optical tweezers.

15 ith high precision under varying loads using optical tweezers.

16 e on the electrode is thus controlled by the optical tweezers.

17 of Kif15 at the single-molecule level using optical tweezers.

18 irpins held under tension in high-resolution optical tweezers.

19 some-bound stalled nascent polypeptides with optical tweezers.

20 e-by-stage complexity by simply removing the optical tweezers.

21 ttering, measuring zeta potential, and using optical tweezers.

22 n, and confirm that this effect occurs using optical tweezers.

23 ces using polystyrene beads manipulated with optical tweezers.

24 ( approximately 50 piconewtons) applied via optical tweezers.

25 cules has been studied using force-measuring optical tweezers.

26 ssenger RNA hairpins tethered by the ends to optical tweezers.

27 emperatures between 7 and 45 degrees C using optical tweezers.

28 rement in a Brownian noise-limited dual-trap optical tweezers.

29 nd thus cannot be trapped using conventional optical tweezers.

30 desired DNA sequences from any organism with optical tweezers.

31 icles through artificial pores and arrays of optical tweezers.

32 ction endonuclease Sau3AI were measured with optical tweezers.

33 es were interrogated by stretching them with optical tweezers.

34 oscopy and while trapping individual GUVs in optical tweezers.

35 th other single-molecule techniques, such as optical tweezers.

36 forces, which have been mostly restricted to optical tweezers.

37 errors in measurements of folding made using optical tweezers.

38 cles on a colloidal monolayer substrate with optical tweezers.

39 quantum and classical information science or optical tweezers.

40 rimental data of single protein folding from optical tweezers.

41 cal force at the single-molecule level using optical tweezers.

42 imeric chaperone Hsp90 using single-molecule optical tweezers.

43 e membrane tether length measured with laser optical tweezers (10.6 +/- 1.1 microm for stem cells, an

44 Similar to optical tweezers, acoustic tweezers have been proposed t

45 a range of constant stretching forces using optical tweezers, allowing direct characterization of th

46 ing both ends through attached beads held by optical tweezers, allowing us to record the applied forc

47 n two DNA-coated microspheres in an extended optical tweezer, allows the study of single duplex DNA m

48 The use of optical tweezers also enables the directed deposition an

49 cal counterparts (10,000,000 times less than optical tweezers and 100 times less than optoelectronic

50 e 'motor' on bare DNA, using high-resolution optical tweezers and a 'tethered' translocase system.

51 Using optical tweezers and a quadrant photodetector, we invest

52 s and inhomogeneous levan-DNA mixtures using optical tweezers and a rotational rheometer.

53 ave developed a noninvasive flow probe using optical tweezers and a viscous flow model in order to de

54 le-molecule manipulation techniques, notably optical tweezers and AFM.

55 he single molecule results obtained by using optical tweezers and also discuss the mechanistic implic

56 Optical tweezers and atomic force microscopy-based singl

57 ation of circular dichroism, thermodynamics, optical tweezers and calorimetry techniques.

58 nding, double-stranded DNA is stretched with optical tweezers and exposed to ligand under constant ap

59 eloped a single-molecule technique combining optical tweezers and fluorescence microscopy that allows

60 rameters of fibrin and used a combination of optical tweezers and fluorescence microscopy to measure

61 FP technology in plant endomembranes, namely optical tweezers and forward genetics approaches, which

62 esis by individual ribosomes using dual-trap optical tweezers and observe simultaneous folding of the

63 include the manipulation of living cells by optical tweezers and optical cooling in atomic physics.

64 Using single molecule optical tweezers and R1597W, we measured the force depen

65 protein (ACBP) in the low-force regime using optical tweezers and ratcheted molecular dynamics simula

66 FP into different conformational states with optical tweezers and simultaneously probing the chromoph

67 ers that combine the nanometer resolution of optical tweezers and the easy manipulation of magnetic t

68 ent of the mechanical state of a system with optical tweezers and the localization of molecular chang

69 ting on single molecules of duplex DNA using optical tweezers and video fluorescence microscopy.

70 FM) or a scanning electron microscope (SEM), optical tweezers, and focused electron-beam nanomanipula

71 we use single-molecule unfolding studies by optical tweezers, and frameshifting assays to elucidate

72 The bead was trapped by optical tweezers, and HMM-thin filament interaction was

73 Here, we combine fluorescence microscopy, optical tweezers, and microfluidics to visualize the ass

74 cted is positioned in one microchannel using optical tweezers, and the transfection agent is located

75 ts-the most common biological application of optical tweezers-and may guide the development of more r

76 l (SOA) on a droplet suspended in an aerosol optical tweezers (AOT).

77 Using an optical tweezers apparatus, we demonstrate three-dimensi

78 Here we use a single-molecule optical tweezers approach to induce the selective unfold

79 Using a novel, single DNA molecule, dual-optical tweezers approach we show how Rad54 stimulates D

80 Plasmonic optical tweezers are a ubiquitous tool for the precise m

81 imental single-molecule curves obtained with optical tweezers are also presented, and they show remar

82 Optical tweezers are excellent tools for transporting pa

83 Optical tweezers are now being used in the investigation

84 Dual-trap optical tweezers are often used in high-resolution measu

85 n of single motor proteins, such as FRET and optical tweezers, are limited to a resolution of approxi

86 ed on atomic force microscopy or magnetic or optical tweezers, are powerful but limited in their appl

87 molecule manipulation methods, in particular optical tweezers, are shedding new light on the molecula

88 We have extended the principle of optical tweezers as a noninvasive technique to actively

89 Our work establishes laser-cooled atoms in optical tweezers as a promising route to bottom-up engin

90 ploratory study to assess the use of aerosol optical tweezers as an instrument for sampling and detec

91 Here we demonstrate an optical tweezer assay to measure the rupture force betwe

92 Here, using a single-molecule optical tweezers assay on mRNA hairpins, we find that th

93 We have developed an optical tweezers assay to follow individual Pol II compl

94 Here, we use a dual-beam optical tweezers assay to study the mechanics of this in

95 Using a single-molecule dual-trap optical-tweezers assay combined with a novel method to e

96 Using an optical-tweezers assay, we measured the binding kinetics

97 Although optical tweezers based on far-fields have proven highly

98 We present a holographic optical tweezers based technology to accurately generate

99 ntinuous wave (CW) and femtosecond plasmonic optical tweezers, based on gold-coated black silicon.

100 iving cells by combining micropatterning and optical tweezers-based active microrheology.

101 The application of holographic optical tweezers-based micromanipulation will enable nov

102 Here, we develop an optical tweezers-based single-molecule (SM) motility ass

103 at low photon flux, while femtosecond-laser optical tweezers can probe the nonlinear optical propert

104 cular force spectroscopic approaches such as optical tweezers can track the pseudoknot's unfolding in

105 hniques has led to the recent development of optical tweezers capable of resolving the motions of bio

106 o resolve rotations during stepping, we used optical tweezers combined with an optical microprotracto

107 Using near-infrared optical tweezers combined with TIRF microscopy, we were

108 Here we show that optical tweezer-controlled pulling of the A1 domain of V

109 ventional manipulation techniques--including optical tweezers, electrokinetic forces (electrophoresis

110 Microrheology using optical tweezers enabled us to measure local viscoelasti

111 be uniquely characterized by this high-force optical tweezers experiment.

112 ging from hairpins to ribozymes, using laser optical tweezer experiments have begun to reveal the fea

113 that in the force range accessible in laser optical tweezer experiments there should be a switch in

114 an integrated approach using single molecule optical tweezer experiments, loop insertions, and steere

115 ding rates comparable to those used in laser optical tweezer experiments, the hairpin is plastic, wit

116 s show excellent quantitative agreement with optical tweezer experiments.

117 Here, we use single-molecule optical tweezers experiments and stochastic theory to sh

118 Optical tweezers experiments have shown that it requires

119 The results of the optical tweezers experiments highlight the complex natur

120 In this work, optical tweezers experiments of cell mechanical properti

121 Single molecule optical tweezers experiments show that under intermediat

122 Based on force-clamp optical tweezers experiments we report here that, in a p

123 elastic deformations match those obtained in optical-tweezers experiments.

124 the packaging process compatible with recent optical-tweezers experiments.

125 we describe a single-molecule assay based on optical tweezers, fluorescence microscopy and microfluid

126 laser ablation unit for microsurgery and an optical tweezer for cell micromanipulation.

127 Use of optical tweezers for quantitative molecular-scale measur

128 Using optical-tweezers force spectroscopy, we found that CS2 f

129 Optical tweezers has emerged as a powerful tool to study

130 , and in the intervening years, the field of optical tweezers has grown tremendously.

131 ion of individual RNA molecules, using laser optical tweezers, has made it possible to infer the majo

132 Optical tweezers have become one of the primary weapons

133 Optical tweezers have become powerful tools to manipulat

134 s inherent in constructing a precision axial optical tweezers have been solved.

135 For example, in free space, optical tweezers have been widely used to manipulate ato

136 Optical tweezers have broad applications in studies of s

137 Among the various tools available today, optical tweezers have recently seen great progress in te

138 Optical tweezers have revolutionized our understanding o

139 Holographic optical tweezers (HOTs) enable the ability to freely con

140 id stain; (ii) capturing a single spore with optical tweezers; (iii) simultaneously measuring phase-c

141 portant implications for the expanded use of optical tweezers in biochemical research and thus should

142 Active microrheology measurements using optical tweezers in living cells reveal that the presenc

143 In order to adopt plasmonic optical tweezers in real-world applications, it is essen

144 Latex micrometric beads are manipulated by optical tweezers in the vicinity of an ultramicroelectro

145 We simulate RBC stretching tests by optical tweezers in three dimensions.

146 e successful approach has been the dual-trap optical tweezers, in which the system of study is held a

147 re similar to those obtained on P pili using optical tweezers, indicating that these are conserved pr

148 hough previous studies have established that optical tweezers induce photodamage in live cells, the e

149 We used an optical tweezers instrument to stretch single lambda-DNA

150 Experimental variables of optical tweezers instrumentation that affect RNA folding

151 Optical tweezers integrated with Raman spectroscopy allo

152 show that immobilizing non-adherent cells by optical tweezers is sufficient to achieve optical resolu

153 Here we show how, using a mobile optical tweezer, it is possible to prepare and locally e

154 he assay is based on an instrument combining optical tweezers, light and fluorescence microscopy, and

155 In single-molecule laser optical tweezer (LOT) pulling experiments, a protein or

156 ost common force spectroscopy techniques are optical tweezers, magnetic tweezers and atomic force mic

157 y used force spectroscopy techniques, namely optical tweezers, magnetic tweezers, and atomic force mi

158 Here, we present the results of optical tweezer measurements that compare the kinetic an

159 Here we report optical tweezers measurements of single DNA molecule pac

160 Here, we present optical tweezers measurements of single DNA molecule pac

161 the motor mechanism we used single-molecule optical tweezers measurements to study the effect of mut

162 By combining single-molecule optical tweezers measurements with a quantitative mfold-

163 predictions of hygroscopicity, as well as to optical tweezers measurements, are presented, demonstrat

164 ckaging dynamics in bacteriophage phi 29 via optical tweezers measurements.

165 A dual optical tweezers method can hence be reliably used to as

166 ing in bacteriophage phi29 using an improved optical tweezers method that allows DNA translocation to

167 We use optical tweezers microrheology and fluorescence microsco

168 In this study, we pioneered the use of optical tweezers microscopy to study Ab-capsule interact

169 Subsequently, direct stretching by optical tweezers of the initial equilibrium shape is sim

170 operating a single-beam gradient force trap (optical tweezers) on an inverted fluorescence microscope

171 Using optical tweezers operated independently of three-dimensi

172 on the membrane of polymersomes using either optical tweezers or a micropipette.

173 this trapping force is comparable to that of optical tweezers or dielectrophoretic traps, without req

174 el were observed in the case of NMIIB-HMM in optical tweezers or TIRF/in vitro motility experiments.

175 Using optical tweezers (OT) and steered molecular dynamic simu

176 Using mechanical unfolding by optical tweezers (OT) and steered molecular dynamics (SM

177 scattering (LS) analysis of capsular PS and optical tweezers (OT) to explore the architecture of the

178 Optical tweezers (OTs) measure the force-dependent time-

179 meter-sized beads of glass held in air by an optical tweezer, over a wide range of pressures, and we

180 Bead probing with optical tweezers provides a new, nondestructive method t

181 The manipulation of proteins with optical tweezers requires attaching molecular handles to

182 igital microfluidics, digital bioassays, and optical tweezers, resulting in a powerful dynamic microw

183 Force-extension experiments with optical tweezers revealed persistence lengths of 1.5 mum

184 Using single-molecule optical tweezers, Sen et al. show that ClpX uses a coord

185 , we have developed a novel temperature-jump optical tweezers setup that changes the temperature loca

186 ent spatial directions, which may affect any optical tweezers setup.

187 e the hopping kinetics of RNA hairpins in an optical tweezers setup.

188 ined in a, to our knowledge, novel dual-trap optical-tweezers setup that directly measures forces.

189 mmon source for these couplings in dual-trap optical-tweezers setups: the misalignment of traps and t

190 Both CD and single molecule studies using optical tweezers showed that the two quadruplexes in the

191 Studies using laser optical tweezers showed that, unlike fully differentiate

192 F-actin and titin PEVK domain measured with optical tweezers") shows that the PEVK domain of titin m

193 present the latest progress that has pushed optical tweezers' spatial and temporal resolution down t

194 with diabetic retinopathy (DR) using a dual optical tweezers stretching technique.

195 A dual-beam optical tweezers system is used to extend double-strande

196 Using an optical tweezers system, isolated mammalian chromosomes

197 Using a cell-bead optical tweezers system, we obtained evidence for cell-m

198 ng and force measurements in a compact fiber optical tweezers system.

199 Here, using optical tweezers techniques, we demonstrate that S1 prom

200 uring invasion, and demonstrate the power of optical tweezers technologies in unraveling the blood-st

201 vector assembly requires a minimal number of optical tweezers that allow operations like chain elonga

202 Here, using optical tweezers that can simultaneously resolve two-pho

203 In this work, using novel magneto-optical tweezers that combine the nanometer resolution o

204 a combination of fluorescence microscopy and optical tweezers, that all three structures can exist, u

205 sing either atomic force microscopy or laser optical tweezers) the distribution of unfolding times, P

206 ased P. falciparum merozoites, delivered via optical tweezers to a target erythrocyte, retain their a

207 We used optical tweezers to analyze the effect of jasplakinolide

208 the fiber based detection, we used the fiber optical tweezers to apply a force on a cell membrane and

209 to hundreds of microns by using holographic optical tweezers to apply pN forces to microparticles em

210 Using optical tweezers to apply tensile force to single domain

211 Using optical tweezers to apply tension across the RNA, we mea

212 we use single-molecule force spectroscopy by optical tweezers to assess the folding and stability of

213 while monitoring DNA unwinding activity with optical tweezers to capture the entire sequence of prote

214 orescence microscopy, Raman spectroscopy and optical tweezers to characterize the germination of sing

215 To study this possibility, we used optical tweezers to determine and compare the structure

216 We have used optical tweezers to determine at the single-molecule lev

217 We use high-resolution optical tweezers to determine the effect of nucleotide a

218 We applied optical tweezers to directly measure single-molecule mec

219 In contrast to raster assembly that assigns optical tweezers to each particle, vector assembly requi

220 Here we use optical tweezers to examine force generation by single m

221 Here, we use optical tweezers to examine the force-dependent stepping

222 Here, we resolve this controversy by using optical tweezers to extend small 60-64 bp single DNA dup

223 We used optical tweezers to follow the unwinding of double-stran

224 Here, we address these questions using optical tweezers to follow translation by individual rib

225 We demonstrate the use of optical tweezers to manipulate and mix droplets.

226 We used optical tweezers to measure single DNA molecule elongati

227 Here, we used single-molecule optical tweezers to measure the assembly energy and kine

228 In this study, we used laser optical tweezers to measure the membrane mechanics of hu

229 We used optical tweezers to measure the strength and attachment

230 contrast microscopy, Raman spectroscopy, and optical tweezers to monitor a variety of changes during

231 Here we use single-molecule optical tweezers to monitor misfolding reactions of the

232 We discuss the use of optical tweezers to monitor the unfolding activities of

233 Here, we used optical tweezers to observe in a cell-free reconstitutio

234 Using optical tweezers to perform microrheology measurements,

235 Key to this work is our use of optical tweezers to precisely position individual cells

236 on and proofreading, we used high-resolution optical tweezers to probe how DNA-duplex stability affec

237 al. and Maillard et al., use single-molecule optical tweezers to show directly that these molecular m

238 Here we used optical tweezers to show that the free energy cost to fo

239 mains of NC to these two activities, we used optical tweezers to stretch single lambda DNA molecules

240 Here we employ optical tweezers to study individual invasion events for

241 Here, we use dual trap optical tweezers to study single yeast RNA polymerase II

242 Here, we used optical tweezers to study the folding behavior of indivi

243 e used single molecule force spectroscopy by optical tweezers to study the folding of calmodulin in t

244 We have used optical tweezers to study the kinetics and thermodynamic

245 antly, our study demonstrates the utility of optical tweezers to test a role for ligand endocytosis i

246 onstrate that polystyrene beads connected by optical tweezers to the ends of adherent filopodia of J7

247 nge of single-molecule force spectroscopy by optical tweezers to the microsecond range by fast sampli

248 Here, we used high-resolution optical tweezers to track the path of single budding yea

249 this technique, we apply an axial force with optical tweezers to vesicles and explicitly demonstrate

250 ing, which combines force and urea using the optical tweezers, together with traditional protein unfo

251 he folding and unfolding of T4 lysozyme with optical tweezers under a chemo-mechanical perturbation b

252 f the phage T4 gp17 motor by using dual-trap optical tweezers under different conditions of perturbat

253 Optical tweezers use the momentum of photons to trap and

254 A dual optical tweezers was made by splitting and recombining a

255 of the rigidity of mucus and model gels with optical tweezers was used in this context to confirm suc

256 For example, using optical tweezers we bring two droplets, one containing a

257 Using optical tweezers we found that the power stroke sizes of

258 Using optical tweezers we measured the dependence of cleavage

259 While pulling on a single filament using an optical tweezer, we record the progressive transformatio

260 Using optical tweezers, we characterize the MT-binding strengt

261 Using high-resolution optical tweezers, we characterized their energies and tr

262 Using optical tweezers, we demonstrate that arrest of SecM-sta

263 Using optical tweezers, we find that isolated nuclei lacking i

264 Using high-resolution optical tweezers, we find that packaging occurs in incre

265 Using optical tweezers, we found that tensile force further in

266 Using optical tweezers, we found that the adhesion strength of

267 By using optical tweezers, we have investigated the mechanical un

268 Using optical tweezers, we have measured the effect of monoval

269 Using optical tweezers, we investigated the mechanical unfoldi

270 Using optical tweezers, we measure the rates of unwinding and

271 Using optical tweezers, we measured the diffusion coefficient

272 Using optical tweezers, we probe pre-TCR bonding with pMHC at

273 Using optical tweezers, we probed the coupling dynamics betwee

274 ,000 times smaller than that in conventional optical tweezers, we rotate, translate, localize, and as

275 Using optical tweezers, we show that filopodial retraction occ

276 By using optical tweezers, we show that the folding-energy landsc

277 orce microscopy, and force spectroscopy with optical tweezers, we show that these DNA variants have b

278 Here, using optical tweezers, we tested the dynamic physical interac

279 dimers in a multiequilibria mixture, whereas optical tweezers were applied to monitor the (un)folding

280 In this paper, optical tweezers were implemented on a digital microflui

281 In this study, optical tweezers were used as a single-molecule force tr

282 esults was carried out by calibration of the optical tweezers when trapping microspheres with a diame

283 i.e., without microbead handles) in the dual optical tweezers where they were observed to adopt a "si

284 Here we consider FRs in dual-trap optical tweezers where two different forces (one per tra

285 ing "topological tweezers," an array of weak optical tweezers which strain the lattice by weakly pull

286 Axial optical tweezers, which apply force to a surface-tethere

287 or the spatial position of a bead trapped in optical tweezers, which enables us to reconstruct its dy

288 y a ring of particles trapped in holographic optical tweezers, which form a flexible elastic wall.

289 prising two independent modules: holographic optical tweezers, which offer a versatile and precise wa

290 e is based on fast, real-time control of 100 optical tweezers, which we use to arrange atoms in desir

291 nticipate that these high-resolution magneto-optical tweezers will be instrumental in studying the in

292 Here we integrate optical tweezers with cell biological and biochemical me

293 ts of rates and energy landscapes made using optical tweezers with estimates obtained from the same s

294 combines contactless levitation with aerosol optical tweezers with isotopic exchange (D2O/H2O) to mea

295 We have developed an approach combining optical tweezers with light-sheet microscopy to probe th

296 Using optical tweezers with single base pair (bp) resolution,

297 Combining optical tweezers with single molecule fluorescence offer

298 Here, we used optical tweezers with single-molecule fluorescence to ob

299 Here, we use optical tweezers within a microfluidic system to measure

300 single DNA hairpin molecule was monitored by optical tweezers within a yoctoliter volume.