ライフサイエンスコーパス: optogenetic

1 lar functions with light sensitive proteins (Optogenetics).

2 to label D2R+ neurons for calcium imaging or optogenetics.

3 ch-clamp recordings with calcium imaging and optogenetics.

4 a novel tool for flexible, high-conductance optogenetics.

5 an be used as efficient inhibitory tools for optogenetics.

6 Optogenetics, a widely used technique in neuroscience re

7 ns, such as KillerRed, permit spatiotemporal optogenetic ablation with low-power laser light.

8 pain, unpredictable chronic mild stress, and optogenetic ACC stimulation and were evaluated using nov

9 he SIAs depolarize during flipping and their optogenetic activation induces flipping in a fraction of

10 Here, we studied the impact of phasic optogenetic activation of 5-HT neurons in mice over time

11 re, we show in mice that food deprivation or optogenetic activation of AgRP neurons induces feeding t

12 erting response that can also be elicited by optogenetic activation of alpha'3 MBONs.

13 The selective optogenetic activation of ARN(KISS) neurons for 1 min ge

14 Accordingly, optogenetic activation of ChCs rapidly suppresses BLAPCs

15 Optogenetic activation of CRFR2 neurons in the pBNST dec

16 We found that rhythmic optogenetic activation of CT V1 neurons dramatically ind

17 hat TBS evokes corticostriatal LTP, and that optogenetic activation of D1R-SPNs during induction impa

18 Additional experiments demonstrate that optogenetic activation of D1R-SPNs reduces stimulation-e

19 th LTP induction and dopamine release during optogenetic activation of D1R-SPNs.

20 When combined with optogenetic activation of defined presynaptic neurons, C

21 synaptic transmission in vivo using targeted optogenetic activation of DG granule cells while recordi

22 Optogenetic activation of direct pathway striatal projec

23 In addition, optogenetic activation of Drd3(LS) neurons rescues ELS-i

24 Optogenetic activation of inferior olivary axons in vitr

25 Optogenetic activation of L6 CT neurons could increase o

26 The high-throughput assay employs optogenetic activation of neurons when the fly occupies

27 urface, achieving broader and more efficient optogenetic activation of neurons, compared to standard

28 We show that optogenetic activation of NPF neuron is rewarding in olf

29 Optogenetic activation of parafascicular inputs evokes s

30 e, we report that selective chemogenetic and optogenetic activation of PBel(CGRP) neurons caused wake

31 Optogenetic activation of Piezo2(+) vagal sensory neuron

32 Dopamine increases driven by optogenetic activation of prefrontal cortex (PFC) termin

33 These observations indicate that optogenetic activation of the beta-cells propagates to t

34 Optogenetic activation of the pathway in acute mouse bra

35 Optogenetic activation of the sympathetic nervous system

36 After optogenetic activation of these neurons, mice drank wate

37 Optogenetic activation of these synapses in vitro evoked

38 Optogenetic activation of this monosynaptic craniofacial

39 ional deficits that are rescued by selective optogenetic activation of this pathway.

40 Optogenetic activation of this projection elicits monosy

41 Optogenetic activation of vM1 supra-linearly amplified t

42 e of cue presentation temporarily ceased and optogenetic activation of VMS D2-MSNs facilitated acquis

43 mal amplification occurred when onset of vM1 optogenetic activation preceded vibrissa stimulation by

44 eries, pharmacology and ion replacement, and optogenetic activity studies.

45 Subsequent optogenetic analyses revealed increased depression of IP

46 Next we use optogenetic and chemogenetic approaches to show that dor

47 Both optogenetic and chemogenetic stimulation of central amyg

48 we used anterograde and retrograde tracers, optogenetic and DREADD-assisted circuit mapping, and cir

49 ify candidate markers for these neurons, and optogenetic and pharmacogenetic manipulations demonstrat

50 Here, we used optogenetic and transgenic approaches to prevent transie

51 , ultrastructural analysis, calcium imaging, optogenetics and behavioral analyses, we uncovered a cir

52 develop in vivo optical sensors, such as for optogenetics and force transduction.

53 Using a variety of anatomical, optogenetic, and in vitro physiological techniques in ma

54 f nociception, using novel transgenic lines, optogenetics, and calcium imaging in behaving larval zeb

55 omputational simulation, two-photon imaging, optogenetics, and dual-color uncaging of glutamate and G

56 ection of cardiomyocyte subpopulations using optogenetics, and opens new frontiers of exploration int

57 P-16.48 Using a combination of pharmacology, optogenetics, and phenotypic analyses we determine that

58 iod activity modulation via odorant stimuli, optogenetics, and transgenic tetanus toxin neurotransmis

59 , we propose potential solutions for in vivo optogenetic applications in living organisms with near-i

60 nding and photodissociation, both useful for optogenetic applications.

61 design of novel light-driven ion pumps with optogenetic applications.

62 eric ChR variant with optimal properties for optogenetic applications.

63 rmore, excessive Ca(2+) influx induced by an optogenetic approach also inhibits axon growth.

64 This optogenetic approach could also be useful to explore the

65 An optogenetic approach has the potential to provide single

66 Using electrical stimulation and an optogenetic approach in brain slices from the mouse, we

67 Here we used an optogenetic approach in mice to selectively target and c

68 Here, using an optogenetic approach involving an opsin to activate the

69 Here we employ an optogenetic approach to explore the effect of large-scal

70 We took a unique optogenetic approach to induce and locally target a roto

71 We used an optogenetic approach to recruit motor proteins to cargo

72 To test this model, we used an in vitro optogenetic approach to stimulate select populations of

73 Using a closed-loop optogenetic approach, we found that selective inhibition

74 Using an optogenetic approach, we report here that LFS of two int

75 in vivo using a combined viral-infection and optogenetics approach to drive expression of channelrhod

76 Here we use the optogenetics approach to selectively stimulate neurons i

77 We use here a variety of optogenetic approaches in freely behaving mice to evalua

78 To extend optogenetic approaches to the immune system to remotely

79 etic analysis, molecular-activity probes and optogenetic approaches to uncover the molecular mechanis

80 To test the predictions of the optogenetic assay, we examined the contribution of dynei

81 Here, we assess the efficacy of optogenetic atrial tachycardia (AT) termination in human

82 R", is particularly promising for inhibitory optogenetics because of its combination of larger curren

83 binatorial screen in yeast, we engineered an optogenetic biosensor, GIBBERELLIN PERCEPTION SENSOR 1 (

84 Restraint stress or optogenetic C1 neuron (C1) stimulation (10 min) protecte

85 Using optogenetics, cell-specific ablation, whole cell patch-c

86 We paired optogenetic channelrhodopsin (ChR2) stimulation in eithe

87 ive, ultrasound-based technique to introduce optogenetic channels into the brain by temporarily openi

88 on of pharmacological, electrophysiological, optogenetic, chemogenetic, and functional magnetic reson

89 Optogenetic, chemogenetic, genetic, and pharmacological

90 Here, using optogenetic circuit analysis and cell type-specific deep

91 ranging from selective genetic targeting to optogenetic circuit control to mapping whole connectomes

92 hemistry, acute slice electrophysiology, and optogenetic circuit mapping, we reveal that this novel i

93 zebrafish embryos and its application to the optogenetic control of cell signaling.

94 This allows bidirectional optogenetic control of cellular activity triggered by Ch

95 Optogenetic control of individual neurons with high temp

96 Optogenetic control of Rho-kinase combined with computat

97 Here we combine optogenetic control of RhoA, live-cell imaging and tract

98 holography (2P-CGH) recently demonstrated 3D optogenetic control of selected pools of neurons with si

99 ble cells, mitochondria have thus far eluded optogenetic control.

100 Optogenetic counteraction of those changes in the VLS an

101 We also demonstrated that chemogenetic or optogenetic depolarization of GABAergic dorsal root gang

102 Optogenetic disruption of cortical activity before and d

103 Optogenetic dissection of inputs originating from dorsal

104 Optogenetic drive of IN inputs to the RNm revealed stron

105 Moreover, two-photon optogenetics enable the possibility of artificially impr

106 ls (hypomethylated), was targeted with these optogenetic-epigenetic constructs.

107 We show that their optogenetic excitation evokes rapid protective and avoid

108 gs alongside pharmacological inactivation or optogenetic excitation to show that GHT signalling activ

109 Moreover, perturbative optogenetic experiments revealed that the inhibitory con

110 nts move faster than wild-type worms and, by optogenetic experiments, contract more.

111 introduce dynamic causal modeling (DCM) for optogenetic fMRI experiments-which uniquely allow cell-t

112 so, it suggests the biological importance of optogenetic follow-up of less-publicized electrical stim

113 Finally, we employed CaMPARI and optogenetics for functional circuit mapping in ex vivo a

114 brane potential with light is fundamental to optogenetics for research and clinical applications.

115 Despite common use of ChR2 in optogenetics for selective control and monitoring of ind

116 Finally, we discuss how optogenetic functional magnetic resonance imaging reveal

117 Here, we describe an optogenetic gene expression system optimized for use in

118 ith the many molecular options available for optogenetic gene expression, we view this method as a ve

119 KEY POINTS: Optogenetics has emerged as a potential alternative to e

120 rial version phycocyanobilin, often used for optogenetics, has a dramatically stabilized Pfr state.

121 Recent advances in optogenetics have opened new routes to drug discovery, p

122 Optogenetic hyperpolarization of interneurons had spatia

123 in the SNc controls mouse behavior, we used optogenetics in awake behaving mice and found that activ

124 probes for advanced in vivo pharmacology and optogenetics in freely moving rodents.This protocol is a

125 bining quantitative behavioral analysis with optogenetics in the head-fixed setup, we established a n

126 al modeling, two-photon calcium imaging, and optogenetic inactivation in mice.

127 CS presentation was discretely coupled with optogenetic inhibition (5-s laser activation) or no opto

128 Moreover, optogenetic inhibition and ablation of VLS D2-MSNs cause

129 Further analysis revealed that optogenetic inhibition of 5-HT inputs into the dBNST inc

130 We show that brief optogenetic inhibition of BLA neurons around moments of

131 pects of learning in rats to show that brief optogenetic inhibition of BLA neurons around moments of

132 ent, we use a novel GAD-Cre rat to show that optogenetic inhibition of LH gamma-aminobutyric acid (GA

133 Optogenetic inhibition of lOFC to BLA, but not BLA to lO

134 We found that optogenetic inhibition of nociceptive sensory afferents

135 el(CGRP) neurons caused wakefulness, whereas optogenetic inhibition of PBel(CGRP) neurons prevented a

136 Optogenetic inhibition of PBel(CGRP) terminals identifie

137 Optogenetic inhibition of pBNST CRFR2 neurons yielded op

138 c lesions of the RMTg or temporally specific optogenetic inhibition of RMTg efferents in the ventral

139 Here we show that optogenetic inhibition of the BLA has opposite effects o

140 Here, we show that pathway-specific optogenetic inhibition of the nucleus prepositus caused

141 oned place preference procedure to show that optogenetic inhibition of the ventral pallidum (VP), a r

142 cocaine-seeking behavior relative to (i) no optogenetic inhibition or (ii) manipulations in mCherry

143 ased freezing, whereas both chemogenetic and optogenetic inhibition reduced freezing.

144 Optogenetic inhibition showed that this resistance to pu

145 Focal optogenetic inhibition tiled across cortex revealed a cr

146 etic inhibition (5-s laser activation) or no optogenetic inhibition while lever responding was assess

147 Using contextual fear conditioning and optogenetic inhibition, we show that DG contributes to b

148 core learning process disrupted by brief BLA optogenetic inhibition.

149 Optogenetic interference with CT feedback to LGN during

150 Optogenetic interrogation of neural pathways relies on d

151 d anatomical tracing of mPFC projections and optogenetic interrogation of their synaptic connections

152 In contrast to optogenetic interventions, transcranial electrical stimu

153 In this study, we use a combination of optogenetic intracellular inputs, CRISPR-based knockouts

154 Optogenetics is a powerful research approach that allows

155 Despite recent advances in optogenetics, it remains challenging to manipulate gene

156 With the rise of ChR2 use in optogenetics, it will be critical to identify residues t

157 Trial-by-trial correlational analysis and optogenetic manipulation demonstrate that facilitation i

158 trophysiological recording and cell-specific optogenetic manipulation in both anesthetized and non-an

159 of its demonstrated potential as a tool for optogenetic manipulation of cell-signaling pathways invo

160 receptors on dopamine or striatal neurons or optogenetic manipulation of dopamine concentration alter

161 l interneurons with channelrhodopsin for the optogenetic manipulation of hippocampal circuitry with l

162 Optogenetic manipulation of the ventral tegmental area d

163 could be blocked or be mimicked by selective optogenetic manipulation of these circuits.

164 Furthermore, bidirectional optogenetic manipulation of these neurons reveals that s

165 These results pave the way for optogenetic manipulation with the spatial and temporal s

166 nnel for retrograde labelling, bidirectional optogenetic manipulation, and optrode recording, we show

167 ectrophysiological recordings and concurrent optogenetic manipulations are improving our understandin

168 Second, optogenetic manipulations confirm that entrainment is un

169 Using cell-type-specific optogenetic manipulations in behaving animals, we show t

170 argeted electrophysiological recordings, and optogenetic manipulations, we show that putatively feedb

171 eyond 700 nm would generate new prospects in optogenetics, membrane sensor technology, and complement

172 upling of spiking to slow oscillations using optogenetic methods eliminated rescaling.

173 d spatial control by the use of chemical and optogenetic methods for inducing hook-anchor attachment.

174 d cell-type-specific anterograde tracing and optogenetic methods to selectively label and manipulate

175 In vitro electrophysiological/optogenetic methods were used to examine responses of MG

176 We demonstrate here, with anatomical and optogenetic methods, and using both rats and mice (of bo

177 CC) correlated with noxious intensities, and optogenetic modulation of ACC neurons showed bidirection

178 Optogenetic neuronal network manipulation promises to un

179 R/Cas9 gene editing, inducible technologies, optogenetic or DREADD cellular control, and cell type-sp

180 rolling the activity of select neurons using optogenetic or thermogenetic tools.

181 uggest that detailed computational models of optogenetic perturbations are necessary to interpret the

182 By combining anatomical tracing and optogenetic/pharmacological manipulations, we discovered

183 Here, we introduce an optogenetic platform that uses light to activate IDR-med

184 Optogenetics promises to deepen our understanding of how

185 In vivo optogenetics provides unique, powerful capabilities in t

186 atment displaced PI3K C2A from the Golgi and optogenetic recruitment of the PI3K C2A kinase domain to

187 ramatically impairs exercise capacity, while optogenetic recruitment of the same neuronal population

188 Pharmacological or optogenetic reversal of these adaptations have been show

189 ally over the past decade, spurred on by the optogenetic revolution.

190 Optogenetic self-stimulation of acid-sensing TRCs in thi

191 y to selectively target CRF neurons with the optogenetic silencer archaerhodopsin tp009 (CRF-ArchT) t

192 Rapid optogenetic silencing and electrical stimulation indicat

193 SPW-R detection at goal locations to trigger optogenetic silencing of a subset of CA1 pyramidal neuro

194 Optogenetic silencing of CeAL CRF neurons during context

195 Finally, optogenetic silencing of existing DGCs during novel envi

196 In mice, optogenetic silencing of IPN neurons increases salience

197 These results suggest that selective optogenetic silencing of nociceptive bladder afferents m

198 Here, we show that the optogenetic silencing of the VH prevented the recall of

199 nd behaving mice, we examined the effects of optogenetic silencing of TMNv HA neurons in vivo We foun

200 voidance behaviors and stress calls, whereas optogenetic silencing specifically reduced facial nocice

201 esponsivity to foot shock were unaffected by optogenetic silencing.

202 del thus offers a theoretical account of how optogenetic stimulation alters the excitability of corti

203 avioural assays, volumetric calcium imaging, optogenetic stimulation and circuit modelling to reveal

204 ictable chronic mild stress and repeated ACC optogenetic stimulation and is reversed by fluoxetine.

205 iode (LED)-based fluorescence microscopy and optogenetic stimulation as well as a Peltier-based tempe

206 We show that targeted optogenetic stimulation based on analysis of AT morpholo

207 We show that targeted optogenetic stimulation based on automated, non-invasive

208 tonic currents, and that both electrical and optogenetic stimulation can evoke NMDA-mediated synaptic

209 ptors to their axon terminals in the EB, and optogenetic stimulation coupled with electrophysiologica

210 Optogenetic stimulation designed to saturate LTD produce

211 Importantly, we also show that LDT-VTA optogenetic stimulation is reinforcing, and that iuGC an

212 his spatiotemporal dynamic using closed-loop optogenetic stimulation is sufficient to increase moveme

213 s from layer V pyramidal neurons showed that optogenetic stimulation normalized cortical hyperexcitab

214 for the anxiolytic effect observed following optogenetic stimulation of 5-HT inputs into the dBNST.

215 Moreover, optogenetic stimulation of axon collaterals of double-pr

216 ing cholinergic levels in the cortex through optogenetic stimulation of basal forebrain cholinergic n

217 Specifically, we used 1 Hz optogenetic stimulation of calcium/calmodulin-dependent

218 Unilateral optogenetic stimulation of cortical pyramidal neurons bo

219 Although optogenetic stimulation of either cell type significantl

220 her, our findings indicate that the targeted optogenetic stimulation of intracellular Ca(2+) signal a

221 We find that optogenetic stimulation of mouse zona incerta (ZI) gamma

222 Furthermore, optogenetic stimulation of mPOA(Nts)-VTA circuitry promo

223 -aspartate microinjection in the SNpc and/or optogenetic stimulation of nigro-vagal terminals in the

224 Indeed, optogenetic stimulation of PPN axons reliably evoked spi

225 IL-17 deficiency can be bypassed by optogenetic stimulation of RMG.

226 Optogenetic stimulation of serotonin neurons in the dors

227 lectrical stimulation of dmPFC layer I or by optogenetic stimulation of specific interneurons ex vivo

228 pproach, our results indicate that selective optogenetic stimulation of TH(VTA) neurons enhanced cere

229 Finally, delta-frequency optogenetic stimulation of thalamic synaptic terminals o

230 Functionally, optogenetic stimulation of these neurons promotes the ac

231 Next, we studied how optogenetic stimulation of these projections affects beh

232 that endogenous NPY, released in response to optogenetic stimulation or synaptically evoked spiking o

233 Constant optogenetic stimulation targeting both pyramidal cells a

234 glutamatergic terminals (lower frequency of optogenetic stimulation to induce glutamate release).

235 rtex as a Wilson-Cowan neural field in which optogenetic stimulation was represented by an external c

236 locally transformed into a type II medium by optogenetic stimulation which predominantly targets inhi

237 at pairing central nucleus of amygdala (CeA) optogenetic stimulation with one option for earning intr

238 t was ipsilateral, but not contralateral, to optogenetic stimulation, suggesting involvement of inter

239 s area reflects their choosing to experience optogenetic stimulation.

240 re-entrant AT (induced via rapid pacing) via optogenetic stimulation.

241 Optogenetic strategies to restore vision in patients who

242 st Saccharomyces cerevisiae, we developed an optogenetic strategy that uses a photoactivatable adenyl

243 We used an optogenetic strategy to isolate identified SNc inputs an

244 portance of paracrine regulation by using an optogenetic strategy.

245 Optogenetic studies have revealed that dopamine neurons

246 Both electrical stimulation and optogenetic studies have shown that increased activity i

247 ites and connectivity between electrical and optogenetic studies of core drive and reinforcement site

248 Recent optogenetic studies on memory engram formation assign a

249 s to increase the utility of the protein for optogenetic studies.

250 e a critical axis of manipulation for causal optogenetic studies.

251 We propose that wide-field optogenetic suppression of inhibition under promoters ta

252 In-phase optogenetic suppression of thalamic spindles impaired hi

253 We describe an optogenetic system for activating Erk with high spatiote

254 nd enhance the controllability of CRY2-based optogenetic systems.Cryptochrome 2 (CRY2) can form light

255 and mossy cells in mice that we validated by optogenetic tagging of mossy cells.

256 Localized optogenetic targeting of rotors in atrial monolayers cou

257 Commonly used optogenetic techniques rely on the expression of microbi

258 nities and unresolved challenges in applying optogenetic techniques throughout the discovery pipeline

259 Optogenetic terminal-specific attenuation revealed a cri

260 patient atria to explore the feasibility of optogenetic termination of atrial tachycardia (AT), comp

261 Using in vivo optogenetics, the brain region-specific inputs to the NA

262 eation of new proteins for illuminating, via optogenetics, the fundamentals of brain function.

263 Using optogenetics to augment dopamine concentration, we found

264 We used optogenetics to demonstrate that the pedunculopontine te

265 use genetics, electrophysiology, imaging and optogenetics to directly target major classes of spinal

266 ns of ventral pallidal neurons, we next used optogenetics to examine whether changes in synaptic plas

267 ArchaerhodopsinT3.0 (ArchT) loss-of-function optogenetics to explore BP regulation by C1 neurons in i

268 ve circuit, we used AgRP-neuron ablation and optogenetics to explore connectivity in acute slice prep

269 se anterior cingulate cortex (ACC), by using optogenetics to induce oscillations in activity, can pro

270 A new study deploys optogenetics to induce the yeast bud on demand, at a sit

271 To this end, the current study used optogenetics to inhibit the BLA during specific task pha

272 dy combines neuronal ensemble recordings and optogenetics to map a functional gradient in rodent pref

273 y somatosensory cortex (S1) of mice by using optogenetics to map the connections between parvalbumin

274 egrating personalized immunoengineering with optogenetics to overcome critical hurdles in cancer immu

275 the Cre-recombinase/loxP system in mice with optogenetics to structurally and functionally characteri

276 oduces the precise spatiotemporal control of optogenetics to the molecular control of synaptic functi

277 Here we apply optogenetics to understand how subpopulations of beta-ce

278 on two-photon calcium imaging and two-photon optogenetics, to detect, characterize, and manipulate ne

279 osin VI with this specificity, we created an optogenetic tool for activating myosin VI by fusing the

280 ets in guiding cell migration, we develop an optogenetic tool for Plexin-B1 designated optoPlexin.

281 We developed a novel optogenetic tool, SxIP-improved light-inducible dimer (i

282 e precise and rapid control afforded by this optogenetic tool, together with quantitative computation

283 It has generated excitement as an optogenetics tool for the manipulation of cyclic nucleot

284 Here, we expand the optogenetics toolbox in the form of a tunable, high-cond

285 Optogenetic tools allow regulation of cellular processes

286 timuli, making it an excellent candidate for optogenetic tools based on retinal analog-bound vertebra

287 ystems can be regulated, and demonstrate how optogenetic tools can discern their functional architect

288 Finally, we provide an overview of optogenetic tools developed from phytochromes and descri

289 Microbial opsin-based optogenetic tools have been transformative for neuroscie

290 relevant context, by inducing expression of optogenetic tools in neurons under dopaminergic control

291 Here, we report newly optimized optogenetic tools to induce transcription with light in

292 Here we report optogenetic tools to upregulate and downregulate such fo

293 Optogenetic tools, in particular tools for all-optical e

294 Using optogenetics, we demonstrate that activation of 5-HT ter

295 Using optogenetics, we demonstrate that adult-born granule cel

296 Using optogenetics, we probe yeast polarization and find that

297 , by combining in vivo neural recordings and optogenetics, we unexpectedly find that both suppressing

298 Combining optogenetics with a closed-loop stimulation approach in

299 We combined optogenetics with calcium imaging and pharmacology to de

300 voltammetry, electrophysiology, and in vivo optogenetics with localized pharmacology to identify neu

301 ermed three-dimensional scanless holographic optogenetics with temporal focusing (3D-SHOT), which all