ライフサイエンスコーパス: organoid

1 cultured them as organoids (patient-derived organoids).

2 es, planarians, zebrafish and human cerebral organoids.

3 UWE1 immunofluorescence analysis of cerebral organoids.

4 mitosis, both in primary tissue and in older organoids.

5 robust protocol for high quality intestinal organoids.

6 l conditions can produce outgrowths known as organoids.

7 cytoplasm of TREX1 mutated stem cell-derived organoids.

8 ion of tissue-like structures referred to as organoids.

9 r staging of human stem-cell-derived retinal organoids.

10 tal and differentiative capacity of cerebral organoids.

11 rise to self-organizing structures known as organoids.

12 in vitro growth of intestinal stem cells and organoids.

13 both primary tissue and in stem cell-derived organoids.

14 ects in hNPCs and human fetal-like forebrain organoids.

15 step between definitive endoderm and mature organoids.

16 s appear only or predominantly in late-stage organoids.

17 intestinal epithelium of mice and intestinal organoids.

18 were induced in both WT and Raptor deficient organoids.

19 ed to almost twice the size of nonstimulated organoids.

20 pment, and demonstrate it using mouse kidney organoids.

21 tes human cortical neurogenesis in forebrain organoids.

22 thotopic transplantation of Apc-edited colon organoids.

23 e pre-organoids that matured into intestinal organoids.

24 e embryonic mouse cortex and human forebrain organoids.

25 an pluripotent stem cells and derived kidney organoids.

26 1) and malignant (LNCaP) prostate epithelial organoids.

27 involved in dorso-ventral patterning within organoids.

28 organoids, they had no effect on the benign organoids.

29 and taste transduction elements in cultured organoids.

30 intermediate into functional proximal airway organoids.

31 dependent growth of Trp53/Kdm6a null bladder organoids.

32 ns for the production of cell aggregates and organoids.

33 tly larger and substantially folded cerebral organoids.

34 ellets remodelled into miniature bone/marrow organoids.

35 pluripotent stem cell-derived multicellular organoids.

36 ed growth of intestinal epithelial cells and organoids.

37 plasia, invasive adenocarcinoma, or prostate organoids.

38 on and causes AJ deficits in human forebrain organoids.

39 patient-derived GSCs grown in culture and in organoids.

40 orters in complex human iPSC-derived retinal organoids.

41 mplications for making more realistic kidney organoids.

42 PCa xenografts and fresh prostate epithelial organoids.

43 d ex vivo, or from patient-derived human CRC organoids.

44 gregated within hours, forming tight retinal organoids.

45 ndividual cells isolated from 31 human brain organoids.

46 hollow lumen morphology in Vitamin E treated organoids.

47 ramming morphogenesis in complex tissues and organoids.

48 e found that by 48 h in culture, the retinal organoids acquire a distinct spatial organisation, i.e.

49 SCs and PDA organoids, and demonstrated that organoid-activated CAFs produced desmoplastic stroma.

50 tiation of human pluripotent stem cells into organoids - aggregates with multiple tissue-specific cel

51 used 3-dimensional small intestine and colon organoids, along with RNA-Seq and gene ontology methods,

52 The premalignant organoids also displayed significant downregulation of g

53 e maintenance of Lgr5(+) IESCs in intestinal organoids, an effect mainly mediated by Gremlin1.

54 g ethics early during the planning stages of organoid and gastruloid research may help prevent future

55 Using mouse in vitro crypt organoid and in vivo models, this study first demonstrat

56 el systems, including the growing variety of organoid and organ-on-a-chip platforms, have so far refl

57 be easily extended to help ensure that human organoid and related research moves forward in an ethica

58 Here the authors use a 3D GC organoid and show EZH2 mediates germinal centre (GC) for

59 This large biobank of 106 tumours, 35 organoids and 59 xenografts, with extensive omics data c

60 id cells, such as pluripotent stem cells, 3D organoids and cell lines, by co-delivery of CRISPR-Cas9

61 taking advantage of this ability, embryoids, organoids and gastruloids have recently been generated i

62 Research involving human organoids and gastruloids involves ethical issues associ

63 r, the intrinsic self-organizing behavior of organoids and gastruloids may pose a slight challenge to

64 s(G12D/+);Trp53Delta/Delta (AKP) mouse colon organoids and human CRC organoids engraft in the distal

65 d into immunocompromised mice, duodenum-like organoids and ileum-like organoids retained their region

66 eneration, and adenoma formation in mini-gut organoids and mice.

67 latform for generating domain-specific brain organoids and modeling human interneuron migration and o

68 ed screens and validation in human forebrain organoids and mouse models in identifying drug candidate

69 Stem cell-derived organoids and other 3D microtissues offer enormous poten

70 al cell state in human cancer cell lines and organoids and show that it depends on a druggable lipid-

71 ty criteria for phenotypic analysis of brain organoids and shows that the spatial and temporal patter

72 and reduced tissue damage in human cortical organoids and the embryonic brain of the ZIKV-induced mo

73 However, the cells generated within organoids and the extent to which they recapitulate the

74 eloped an assortment of bioengineered tissue organoids and tissue constructs that are integrated in a

75 erized the role of ATP7B in mouse intestinal organoids and tissues.

76 aberrant Tuj1(+) neuronal migration in RB-KO organoids and upregulation of the gene encoding VLDLR, a

77 lution, penetration depth through the entire organoid, and functional endpoints.

78 inding in co-cultures of murine PSCs and PDA organoids, and demonstrated that organoid-activated CAFs

79 OMI) is highly sensitive to drug response in organoids, and OMI in tumor organoids correlates with pr

80 In a study of mouse and human cell lines, organoids, and tissues, we found cIAP1 to be required fo

81 manufacturing processes and critical to many organoid approaches.

82 Primary tumor organoids are a robust model of individual human cancers

83 PLC-derived organoids are amenable for biomarker identification and

84 Pluripotent stem cell (PSC)-derived organoids are miniature, three-dimensional human tissues

85 generating human organoids in vitro, and how organoids are now being used as a primary research tool

86 res and metastatic properties of PLC-derived organoids are preserved in vivo.

87 and vulnerabilities of transgenic intestinal organoids as a novel approach to understanding oncogene

88 es in genetically unmodified cells and heart organoids as well as in zebrafish larval brain via combi

89 Here, we use gastruloids, mESC-based organoids, as a model system with which to study this pr

90 and also increased branching in an in vitro organoid assay.

91 essed in neural stem and progenitor cells in organoids at 15 and 28 days of culture.

92 miR-34a/b/c-deficient adenomas formed tumor organoids at an increased rate.

93 a GC-like phenotype can be generated in the organoids at controlled rates, within approximately 1 we

94 urveyed the transcriptome landscape of taste organoids at different stages of growth.

95 escribe here a serum-free, artificial thymic organoid (ATO) system that supports efficient and reprod

96 e of Cell Stem Cell, using murine intestinal organoids, Basak et al. (2017) induce stem cell quiescen

97 Koehler et al. (2017) have developed a human organoid-based approach using basic developmental princi

98 Organoid branching was dependent on stimulation by FGF2,

99 gnals promote hepatocyte maturation in liver organoids, but organoid self-organization requires cell-

100 egy for increasing the anatomical realism of organoids by applying asymmetric cues to mimic spatial i

101 polarized human enterocyte-models and mouse organoids by basolateral incubation with a high concentr

102 n of BMP4-releasing beads in one place in an organoid can break the symmetry of the system, causing a

103 We find that organoids can generate a broad diversity of cells, which

104 ll as in translational research, but whether organoids can truly live up to this challenge is, for so

105 1047R) We find that survival and motility of organoid cells are associated with 4EBP1 and AKT phospho

106 Jag1(Ndr/Ndr) liver organoids collapsed in culture, indicating structural in

107 pluripotent stem cells (hPSCs) into gastric organoids containing fundic epithelium by first identify

108 drug response in organoids, and OMI in tumor organoids correlates with primary tumor drug response.

109 p an effective protocol for deriving colonic organoids (COs) from differentiated human embryonic stem

110 Finally, neuronal activity within organoids could be controlled using light stimulation of

111 Organoids could be developed over extended periods (more

112 age tracing, single-cell RNA sequencing, and organoid culture approaches to show that Lgr5 and Lgr6,

113 Here, we used an organoid culture system to investigate how transcription

114 cently described a novel, near-physiological organoid culture system, wherein primary human healthy l

115 We explain how to establish the immune organoid culture to sustain immune cell proliferation an

116 ial cells, leading to their proliferation in organoid culture.

117 In studies of intestinal organoid cultures and mice with inducible deletion of ME

118 We generated intestinal organoid cultures from a subset of samples and genome-wi

119 Procedures for establishing mCRPC organoid cultures from contemporary patients were recent

120 We established organoid cultures from ISCs stimulated with HGF or EGF a

121 PLC-derived organoid cultures preserve the histological architecture

122 Cornea organoid cultures provide a powerful 3D model system for

123 In stimulated versus unstimulated organoid cultures, elevated IFN-gamma reduced the mRNAs

124 show that iRFP can be readily detected in 3D organoid cultures, FACS analysis and in vivo tumour mode

125 lly recapitulate human disease together with organoid cultures, we have demonstrated that residual ce

126 the growth of a broad range of primary human organoid cultures.

127 We found that mutation accumulation in organoids deficient in the mismatch repair gene MLH1 is

128 Experiments with IEC organoids demonstrated that IEC expulsion did not requir

129 in genome editing, stem cell production, and organoid derivation from stem cells represent a revoluti

130 fluence brain topology, we analyzed cerebral organoids derived from control and MDS-induced pluripote

131 wo-thirds of mice, and can be achieved using organoids derived from genetically engineered mouse mode

132 As a result, prostate cancer cell lines and organoids derived from individuals harboring SPOP mutati

133 We also observed similar deficits in organoids derived from schizophrenia patient induced plu

134 eveals interlineage communication regulating organoid development, and illuminates previously inacces

135 ineage relationships during MGE and cortical organoid development.

136 MET-deficient adenoma organoids did not respond to HGF stimulation, but did re

137 YAMC cells, and mouse and human intestinal organoids, died rapidly in response to TNF.

138 nt, we investigated its function in cerebral organoids differentiated from gene-edited hESCs lacking

139 Gastric and small intestinal organoids differentiated from human pluripotent stem cel

140 In contrast, Pten deletion in mouse organoids does not lead to folding.

141 Therefore, an OMI organoid drug screen could enable accurate testing of dr

142 We investigated intestinal organoid emergence, focusing on measurable parameters of

143 3D organoids enable in vitro human brain development models

144 Microfilament-engineered cerebral organoids (enCORs) display enhanced neuroectoderm format

145 y engineered mouse models (GEMMs), wild-type organoids engineered ex vivo, or from patient-derived hu

146 rganoid transplantation model of human colon organoids engineered to harbor different CRC mutation co

147 sed by the Zika virus (ZIKV) outbreak, brain organoids engineered to mimic the developing human fetal

148 n vivo contractile tissues, such as cardiac "organoids," engineered heart tissues, and zebrafish and

149 ta (AKP) mouse colon organoids and human CRC organoids engraft in the distal colon and metastasize to

150 ng tubules in three-dimensional human dermal organoid ex vivo culture.

151 While self-organizing organoids excel at recapitulating early developmental ev

152 We investigated adenoma organoid expansion stimulated by HGF or EGF using adenom

153 Mouse organoid experiments point to an intrinsic role for Ret

154 eful and considered view of the state of the organoid field and its current limitations, and lay out

155 Finally, fusing region-specific organoids followed by live imaging enabled analysis of h

156 Systematic inhibitor treatments of organoids followed by quantitative phenotyping and phosp

157 o delete key DNA repair genes in human colon organoids, followed by delayed subcloning and whole-geno

158 lso discuss the potential and limitations of organoids for addressing outstanding questions in lung b

159 and highlights the broad utility of cerebral organoids for modeling human neurodevelopmental disorder

160 A mechanistic understanding of early organoid formation is essential for improving the robust

161 th ATII-to-ATI cell transdifferentiation and organoid formation, which were augmented by FZD4 overexp

162 elease system and monitored the cultures for organoid formation.

163 Finally, the organoid-forming capacity of Spop-null murine prostate c

164 marizes the different methods for generating organoids from cells isolated from human and mouse lungs

165 be the generation of MGE and cortex-specific organoids from human pluripotent stem cells that recapit

166 Effective derivation of functional airway organoids from induced pluripotent stem cells (iPSCs) wo

167 echnologies now make it possible to engineer organoids from purified cellular and extracellular compo

168 also describe new approaches to reconstitute organoids from purified cellular components, and discuss

169 We created cerebral organoids from the isogenic iPSCs and found that blockad

170 he propagation of primary liver cancer (PLC) organoids from three of the most common PLC subtypes: he

171 Optical imaging is uniquely suited to assess organoid function and behavior because of its subcellula

172 Three-dimensional organoids generate complex organ-like tissues; however,

173 We studied expansion of organoids generated from crypts and adenomas, stimulated

174 Furthermore, MGE and cortical organoids generated physiologically functional neurons a

175 Finally, liver cancer organoid-generated high-grade tumors exhibited significa

176 integrated over time when DKO prostate tumor organoids grew larger, setting the stage to translate mo

177 Loss of Gpr182 enhanced organoid growth efficiency ex vivo in an EGF-dependent m

178 ta-catenin signalling, and induce intestinal organoid growth in vitro and Lgr5(+) ISCs in vivo.

179 phic influences and potentiated global PanIN organoid growth.

180 The advent of stem cell-derived retinal organoids has brought forth unprecedented opportunities

181 Specialized cell types and organoids have been derived from human pluripotent stem

182 In this Review, we discuss how organoids have been used to identify and characterize st

183 The resulting organoids have remarkable cell type complexity, architec

184 Organoids have the potential to effectively balance betw

185 report the differentiation of human colonic organoids (HCOs) from hPSCs.

186 human pluripotent stem cell-derived cardiac organoids (hCOs).

187 the development of human fundic-type gastric organoids (hFGOs).

188 rescued the neurotoxicity of AGS neurons and organoids, highlighting their potential utility in thera

189 stem cell (PSC) technology, human intestinal organoids (HIOs) with remarkably similarity to the fetal

190 ial applications in imaging human intestinal organoids (HIOs), colon mucosa, and retina.

191 cells (NCCs) and developing human intestinal organoids (HIOs).

192 tablish a novel 3-dimensional (3D) human FTE organoid in vitro model containing the relevant cell typ

193 oliferation and survival in the premalignant organoids in a manner that recapitulated the SELECT resu

194 Existing methods for making kidney organoids in mice yield developing nephrons arranged aro

195 ogy have been essential for generating human organoids in vitro, and how organoids are now being used

196 Corroborating the results in RB-KO organoids in vitro, we observed ectopically localized Tu

197 explore the regenerative potential of these organoids in vivo and demonstrate that ECOs self-organiz

198 The organoid is composed of a bioadhesive protein, gelatin,

199 uripotent stem-cell-derived human intestinal organoids is globally similar to the immature human epit

200 ity, demonstrating that regional identity of organoids is stable after initial patterning occurs.

201 Intestinal organoids lacking ATG16L1 reproduced this loss in Paneth

202 T/mG), we show that Cbl/Cbl-b DKO in mammary organoids leads to hyperactivation of AKT-mTOR signaling

203 poral expression profiles displayed by taste organoids may also lead to the identification of current

204 etry-breaking cues to improve the realism of organoids may have applications to organoid systems othe

205 Moreover, 3D organoid methodology provides an innovative platform for

206 ransition from 2D to 3D cultures and current organoid (mini-brain) cultures are reviewed to give the

207 This powerful human-derived FTE organoid model can be used to study the earliest stages

208 ify crucial functions for RB in the cerebral organoid model of human brain development.

209 A self-organizing organoid model provides a new approach to study the mech

210 potent stem cell (hPSC)-based 3D neocortical organoid model.

211 The symposium, entitled 'Organoids: modelling organ development and disease in 3D

212 -ranging biomedical utilities of PLC-derived organoid models in furthering the understanding of liver

213 Comparison of SPOP-mutant and ERG-fusion organoid models showed evidence of divergent, rather tha

214 Organoid models therefore clarify the paradoxical findin

215 sition in mitotic dynamics can be studied in organoid models.

216 sed TNF-induced cell death in YAMC cells and organoids-most likely by sequestering and degrading cIAP

217 In mouse prostate organoids, mutant SPOP drove increased proliferation and

218 pithelial cells from the small intestine and organoids of mice, which enabled the identification and

219 ptosis and formed three-dimensional cortical organoids of reduced size.

220 loping brain such as three-dimensional brain organoids offer an unprecedented opportunity to study as

221 Intestinal organoids offer great promise for modeling intestinal di

222 bust quantitative technologies for analyzing organoids on a large scale pose severe limitations for t

223 ition in physiological environments, such as organoids or three-dimensional complex matrices.

224 preferentially collapsed DKO prostate tumor organoids over AADKO organoids, which spontaneously disi

225 Here, we discuss the advantages of brain organoids over other model systems to study development

226 CCA biopsies from patients, cultured them as organoids (patient-derived organoids).

227 th monolayer and three-dimensional (cerebral organoid) patient-derived and MeCP2-deficient neuronal c

228 s to precision medicine in the clinic on the organoid platform.

229 In mouse urothelial organoids, PPAR agonism is sufficient to drive growth-fa

230 For studies using organoids, primary enterocytes were isolated from the in

231 6 significantly reduced in vitro basal crypt organoid proliferation and budding, and in vivo signific

232 emonstrated that IL-6-induced in vitro crypt organoid proliferation and crypt budding was abrogated b

233 onstrated that exogenous IL-6 promoted crypt organoid proliferation and increased stem cell numbers t

234 y orthotopic transplantation of liver cancer organoids propagated from primary tumors in the genetic

235 Cerebral organoids recapitulate human brain development at a cons

236 Similarly, HGF-mediated expansion of adenoma organoids required CD44v4-10.

237 The recent increase in organoid research has been met with great enthusiasm, as

238 mass are enriched 1.5- and 3.8-fold for pre-organoids, respectively, thus providing rational guideli

239 iPSCs) self-assembled into three-dimensional organoids, resulting in hepatic gene induction.

240 mice, duodenum-like organoids and ileum-like organoids retained their regional identity, demonstratin

241 g and subsequent 3D reconstruction of entire organoids reveal that many of these regions are intercon

242 epatocyte maturation in liver organoids, but organoid self-organization requires cell-to-cell surface

243 uodenum, whereas longer exposure resulted in organoids similar to ileum.

244 detection, devised methods to compensate for organoid size variability, evaluated performance and sen

245 Adenoma organoids stimulated with EGF or HGF expanded to almost

246 roids bore a resemblance to mammalian tissue organoids synthesized in vitro We identified one of four

247 Our organoid system provides a genetically-tractable tool th

248 This kidney organoid system provides a platform for studies of human

249 Using a 3D B cell follicular organoid system that mimics the GC reaction, we show tha

250 ectives on overcoming limitations of current organoid systems for their future use in ZIKV research.

251 Organoid systems have been developed to model many human

252 therefore, experience in hPSC culture and 3D organoid systems may be necessary for optimal results.

253 ealism of organoids may have applications to organoid systems other than the kidney.

254 From a translational perspective, organoid systems provide exciting new prospects for drug

255 components, focusing on three adult-derived organoid systems.

256 as models for aging and describe how current organoid techniques could be applied to aging research.

257 Organoid techniques provide unique platforms to model br

258 begin by discussing the exciting promise of organoid technology and give concrete examples of how th

259 Organoid technology and organ-on-a-chip engineering have

260 A newly advanced organoid technology makes it possible to create three-di

261 el is necessary to maximise the potential of organoid technology.

262 wn in three-dimensional (3D) matrices formed organoids that consisted of primitive vascular structure

263 ng human pluripotent stem cells to inner ear organoids that harbor functional hair cells.

264 modeling of diseases can benefit from cornea organoids that include multiple corneal cell types and e

265 We found that 13% of spheroids were pre-organoids that matured into intestinal organoids.

266 escribes a methodology for developing immune organoids that partially mimic the B-cell zone of a lymp

267 ix scaffolds to form three-dimensional liver organoids that recapitulated several aspects of hepatobi

268 healthy liver cells form long-term expanding organoids that retain liver tissue function and genetic

269 crest cells into developing human intestinal organoids, thereby restoring ENS cell types and contract

270 oliferation and induced cell death in cancer organoids, they had no effect on the benign organoids.

271 Here we present human iPS cell-derived organoids through sequential rounds of differentiation p

272 ve 3D growth platform, which enabled the FTE organoid to self-organize into a convoluted luminal stru

273 is perspective is to introduce OMI and tumor organoids to a general audience in order to foster the a

274 e nephron progenitor cells (NPCs) and kidney organoids to facilitate applications for tissue engineer

275 Sensitivity of patient-derived organoids to HSP90 inhibitors, in culture and when grown

276 miRNA21 mediates resistance of CCA cells and organoids to HSP90 inhibitors.

277 Bershteyn et al. (2017), use human cerebral organoids to identify specific cellular defects in neuro

278 e provide a perspective on the potential for organoids to serve as models for aging and describe how

279 Finally, we utilized the expanded cerebral organoids to show that infection with Zika virus impairs

280 limitation by exploiting cultured intestinal organoids together with single-cell measurements to eluc

281 rely on in situ gene editing and orthotopic organoid transplantation in mice without cancer-predispo

282 noma sequence in vivo by using an orthotopic organoid transplantation model of human colon organoids

283 es to PSCs in vitro The approach to generate organoids uses a combination of directed differentiation

284 acrine effects, we first established a liver organoid using human induced pluripotent stem cells (iPS

285 toma cell-line cultured as three dimensional organoids, using a fluidised bed bioreactor, together wi

286 Through the orthotopic engraftment of colon organoids we describe a broadly usable immunocompetent C

287 human embryonic stem cell-derived intestinal organoids, we demonstrate that the duration of exposure

288 Using hPSC 3D neocortical organoids, we demonstrate that the effects of cocaine ar

289 evelopment, analyzed by histology, and liver organoids were cultured and analyzed.

290 Kidney organoids were generated from NPCs within 12 d with high

291 hage cell line RAW 264.7, or mouse and human organoids were incubated with second mitochondrial activ

292 When intestinal organoids were stimulated with IL-4, tuft cells and IL-2

293 When region-specific organoids were transplanted into immunocompromised mice,

294 ectomy or sleeve resection or gastric antral organoids) were incubated with interferon gamma (IFNG) o

295 d higher capacity than Troy(-) cells to form organoids, which is considered a stem cell property in v

296 psed DKO prostate tumor organoids over AADKO organoids, which spontaneously disintegrated over time w

297 ibrosis patient-specific iPSC-derived airway organoids with a defect in forskolin-induced swelling th

298 In the past five years, human PSC-derived organoids with components of all three germ layers have

299 or that stabilizes beta-catenin) resulted in organoids with gene expression patterns similar to devel

300 months, the vesicles develop into inner ear organoids with sensory epithelia that are innervated by