ライフサイエンスコーパス: organotypic

1 breaks (DSBs) were significantly reduced in organotypic 3D culture compared to kinetics of repair in

2 hway genes were significantly reduced in the organotypic 3D culture compared with those in 2D culture

3 y of complex DNA lesions were irreparable in organotypic 3D culture.

4 Here, using organotypic 3D cultures, we found that primary human mes

5 he integration and live behavior of pMGLs in organotypic 3D cultures.

6 In organotypic 3D in vitro cultures we found that transferr

7 Consistently, human organotypic 3D skin models using stable STRA6KD HaCaT ce

8 We recently described a novel organotypic 3xTg-AD mouse brain slice culture model with

9 hymal interactions using a three-dimensional organotypic air-liquid interface coculture system.

10 Organotypic airway cultures pinpoint TAp73 as necessary

11 hese findings were further investigated with organotypic and traditional cell cultures.

12 Novel cellular, three-dimensional organotypic, and ex vivo models are considered and the c

13 This is supported by an organotypic assay showing that Mtss1-expressing cells di

14 t cancer outgrowth in three-dimensional (3D) organotypic assays and in the lungs of mice.

15 ponse to HGF both in 2D and 3D physiomimetic organotypic assays; which can be suppressed by inhibitio

16 Organotypic brain explants represent a successful ex viv

17 he invasion of glioblastoma cells through an organotypic brain slice and glioma progression in vivo.

18 Organotypic brain slice culture models provide an altern

19 Using organotypic brain slice cultures generated from embryoni

20 in the neocortical neural stem cell niche in organotypic brain slices from embryonic mice.

21 been demonstrated in cultured neurons and in organotypic brain slices, but not in acute brain slices

22 n higher levels of Tau and p62 aggregates in organotypic brain slices.

23 ]i in individual astrocytes was performed in organotypic brainstem slice cultures and acute brainstem

24 volatile-mediated communication between the organotypic bronchiole and cultures of Aspergillus fumig

25 haracterize the inflammatory response of the organotypic bronchiole to infection.

26 To complement direct infection of the organotypic bronchiole, we present a clickable extension

27 s this challenge, researchers have developed organotypic cancer models (organoids) that combine the 3

28 A 3-dimensional organotypic cell culture model of esophageal maturation

29 In this study, we established organotypic cell cultures of AT explants to study the im

30 Using chemical proteomics and an organotypic cell-based drug screening assay, we determin

31 ebellar granule neurons (CGNs) and PCs in an organotypic cerebellar culture system.

32 Depleting DOCK10 in organotypic cerebellar cultures resulted in dramatic den

33 ctive in promoting precocious myelination in organotypic cerebellar slice cultures, and in vivo in ea

34 after lysolecithin-induced demyelination of organotypic cerebellar slice cultures.

35 was found to be disturbed, and treatment of organotypic cerebellar slices with ceramide significantl

36 Three-dimensional organotypic co-culture assays revealed that WNT2-mediate

37 Novel three-dimensional organotypic co-culture models are being developed to fac

38 ce for our screening-ready three-dimensional organotypic co-culture platform.

39 Organotypic co-cultures bridge the gap between standard

40 Organotypic co-cultures represent an easy, affordable an

41 These findings were also observed with organotypic co-cultures.

42 compartments, we adapted a three-dimensional organotypic coculture model, originally developed to pro

43 ammatory exposures in a dorsal root ganglion organotypic coculture system.

44 ore invasive when grown in three-dimensional organotypic collagen gel cultures.

45 Here we describe the engineering of an organotypic colon cancer model by recellularization of a

46 human bronchial epithelial cells cultured in organotypic conditions closely approximated gene express

47 Organotypic cortical brain slices exposed to ischemic in

48 We used organotypic cortical cultures from rat brains as a biolo

49 ased image analysis in mouse dissociated and organotypic cortical cultures to uncover that synaptic a

50 Similarly, in organotypic corticoamygdalar slice cultures from immatur

51 l epithelial cells and the application of 3D organotypic culture (OTC), a form of tissue engineering.

52 Organotypic culture and human tumour explants were allow

53 ally relevant drugs blocked invasion both in organotypic culture and in animals, suggesting a new tre

54 We then developed a cochlear organotypic culture and video-microscopy approach to mon

55 RNA interference studies both in vivo and in organotypic culture demonstrate that the beta2*-nAChRs a

56 ells by either methylcellulose suspension or organotypic culture induces LMP1 expression prior to Z a

57 e observations illustrate the utility of the organotypic culture model for the investigation of intra

58 el tumor-invasive signature obtained from an organotypic culture model of engineered ESCC.

59 ection of S631G variant into a 3-dimensional organotypic culture model of normal esophageal squamous

60 The rat hippocampal organotypic culture model of post-traumatic epilepsy was

61 epithelial tissues using a three-dimensional organotypic culture model of the mammary duct.

62 This 3D organotypic culture model reproduced key features of des

63 re mutant (Kv2.1W365C/Y380T: Kv2.1 DN) in an organotypic culture model to manipulate channel expressi

64 A unique three-dimensional (3D) organotypic culture model was established; within a dilu

65 have all led to valuable clinical insights, organotypic culture models have emerged as tractable sys

66 The alginate scaffold-based organotypic culture system is a promising, reliable, and

67 We used an organotypic culture system of human fetal testes explant

68 Analysis of spheroid invasion in an organotypic culture system revealed that these "trailbla

69 isms, we present a novel breathing brainstem organotypic culture that generates rhythmic neural netwo

70 imensional culture conditions (physiomimetic organotypic culture).

71 A expression, reduced tumor cell invasion in organotypic culture, and restricted tumor growth in vivo

72 nicotinic activation of alpha7-nAChRs in WT organotypic culture, as well as cell culture, increases

73 When cultured alone in organotypic culture, both basal- and luminal-type breast

74 Using organotypic culture, we found that the loss of axon rege

75 rized architecture in three dimensional (3D) organotypic culture.

76 at postnatal days (P7-P14) and maintained in organotypic culture.

77 dence for the defective DNA damage repair in organotypic culture.

78 sponses as modeled in three-dimensional (3D) organotypic culture.

79 homogenates and of prion-infected cerebellar organotypic cultured slices and decreased the amount of

80 Moreover, ELISA on cerebellar organotypic cultured slices and in vitro conversion assa

81 e of Herpes simplex virus (HSVTK) cerebellar organotypic cultured slices markedly aggravated prion-in

82 analysis procedure for structurally complex organotypic cultures (CALYPSO) applied to fluorescence-b

83 5-positive tumor biopsy specimens (plugs) or organotypic cultures (rafts) consisting of ChHV5-positiv

84 ECs in rigid and compliant three-dimensional organotypic cultures and 2) restores the induction of th

85 use of biomechanically compliant or rigid 3D organotypic cultures and combined them with global micro

86 Furthermore, by using skin organotypic cultures as a model system to monitor the co

87 cellular P. gingivalis in both monolayer and organotypic cultures compared to free antibiotic or poly

88 In this study, we use organotypic cultures derived from transgenic mice induci

89 nd rescues gap junction coupling in cochlear organotypic cultures from connexin-deficient mice that a

90 In the present study, we further exploit organotypic cultures from wild-type and SOD1(G93A) mice

91 Controlling the microenvironment of such organotypic cultures has impact in tissue engineering, c

92 potential decline and is rescued in cochlear organotypic cultures in low potassium milieu, indicating

93 ardial biopsies were harvested and plated as organotypic cultures in the presence or absence of MSC f

94 agging of endogenous proteins in primary and organotypic cultures in vitro and developing, adult, age

95 and knockdown of Scrib in lung explants and organotypic cultures leads to reduced cohesion of lung e

96 elium exists as a stratified tissue in vivo, organotypic cultures may serve as a better model of EBV

97 NA-mediated knock down of Ano1 expression in organotypic cultures of Ano1 WT small intestine.

98 xpression of Kv channels were explored using organotypic cultures of brainstem prepared from P9-P12 r

99 In organotypic cultures of cerebellar slices, brain-derived

100 Finally, organotypic cultures of darkly pigmented human keratinoc

101 Using a combination of genetic approaches, organotypic cultures of embryonic pancreata, and genomic

102 e, we demonstrate that EBV is able to infect organotypic cultures of epithelial cells to establish a

103 rmaceutical agents and treatment regimens on organotypic cultures of human disease to expedite drug a

104 gulated cyclin E in epithelial tissues using organotypic cultures of human mammary epithelial cells a

105 Using organotypic cultures of Madin-Darby canine kidney cells

106 amplification initiates in spinous cells in organotypic cultures of primary human keratinocytes duri

107 d Ca(2+) signaling in astrocyte processes in organotypic cultures of rat hippocampus.

108 Here, we show that when organotypic cultures of rodent hippocampal slices were t

109 rrier in Pelo-deficient mice, we made use of organotypic cultures of skin explants from control and m

110 enyl 153 (PCB153) on the proteome of primary organotypic cultures of the mouse mammary gland.

111 with morpholino antisense oligos in cochlear organotypic cultures revealed a negative correlation bet

112 ting the growth of breast cancer cells in 3D-organotypic cultures that recapitulated pulmonary microe

113 this study, we used isogenic benign mammary organotypic cultures to interrogate the role of mTOR-med

114 , FN enhanced outgrowth in three-dimensional organotypic cultures via a mechanism that is dependent u

115 pression of differentiation markers in these organotypic cultures were analyzed by using immunohistol

116 Treatment of UVB-exposed monolayer or organotypic cultures with trichostatin A, a histone deac

117 alization of beta-catenin in vitro and in 3D organotypic cultures, accompanied by upregulation of WNT

118 tested in three-dimensional tracheospheres, organotypic cultures, air-liquid interface cultures, and

119 Here, by using NP cells, brain organotypic cultures, and in vivo animal models, we inve

120 orted long-term epidermal regeneration in 3D organotypic cultures, and resulted in the manifestation

121 ian cells lines, as well as mouse cerebellar organotypic cultures, and we also show that they exhibit

122 induced in primary astrocytes, neurons, and organotypic cultures, demonstrating that this phenomenon

123 In cell and organotypic cultures, Dkk4 inhibition is eliminated by e

124 solated cells of the ICC lineage and gastric organotypic cultures, hyperglycemia stimulated prolifera

125 In thymic organotypic cultures, the thymocyte-induced reduction in

126 In organotypic cultures, UVB treatment also resulted in inc

127 Using both mammary organoids and 3D organotypic cultures, we show that MMP activity is neces

128 In 3D organotypic cultures, we showed that a spatially distinc

129 h two-colour Ca(2+) and glutamate imaging in organotypic cultures.

130 ld be unlike other herpesviruses examined in organotypic cultures.

131 opment and also upon stimulation in cortical organotypic cultures.

132 drocytes, mixed glial cells, and spinal cord organotypic cultures.

133 ingle epithelial cells and three-dimensional organotypic cultures.

134 rin beta1; and increased cell invasion in 3D organotypic cultures.

135 ammary epithelia in vivo, ex vivo, and in 3D organotypic cultures.

136 cer diagnosis was further demonstrated in an organotypic cutaneous squamous cell carcinomas model, al

137 and cathelicidin [LL-37]) are studied in an organotypic dento-epithelial (OD-E) model exposed to Fus

138 t collagen IV production were examined in an organotypic dento-epithelial (OD-E) model.

139 regenerating tissues and acquire features of organotypic ECs.

140 Uncovering the mechanisms by which organotypic endothelium distributes physiological levels

141 aging of denervated dentate granule cells in organotypic entorhino-hippocampal slice cultures of Thy1

142 blished an in vitro model of rMS using mouse organotypic entorhino-hippocampal slice cultures.

143 nvestigate siRNA uptake by keratinocytes, an organotypic epidermal model, in which pre-existing endog

144 icient to reduce terminal differentiation of organotypic epidermal skin equivalents, indicating overl

145 d a highly productive infection of HPV-18 in organotypic epithelial cultures.

146 ed the gene signature, which was verified in organotypic ex vivo culture of patient-derived fibrotic

147 vation of respective forebrain targets using organotypic explant cocultures of ventral midbrain (VM)

148 Ex vivo transduction of mouse organotypic explants identified Anc80L65 from a set of o

149 entify attributes that confer ECs with their organotypic features.

150 l programs generate cell diversity within an organotypic framework, enabling the later physiological

151 -derived cardiomyocytes and fibroblasts with organotypic functionality under serum-free conditions.

152 and similarly decreasing endogenous TRIO in organotypic hippocampal brain slices significantly incre

153 onal networks were successfully grafted into organotypic hippocampal brain slices, showing an approxi

154 lusions of the study are as follows: (1) the organotypic hippocampal culture model of post-traumatic

155 nt of epilepsy after brain injury, using the organotypic hippocampal culture model of post-traumatic

156 Using rat organotypic hippocampal cultures, we found that IL-1beta

157 We induced tau hyperphosphorylation in organotypic hippocampal slice cultures (OHSC) and applie

158 mage stemming from ROS and RNS production in organotypic hippocampal slice cultures (OHSC), as well a

159 Extracellular fluid collected from rat organotypic hippocampal slice cultures (OHSCs) by electr

160 We have tested this by examining organotypic hippocampal slice cultures (OHSCs) exposed t

161 ethine and CoA in the extracellular space of organotypic hippocampal slice cultures (OHSCs).

162 metric or intensiometric Ca(2+) indicator in organotypic hippocampal slice cultures (one- and two-pho

163 dary assays (i.e. post-treatment protocol in organotypic hippocampal slice cultures and cortical neur

164 Using organotypic hippocampal slice cultures and primary neuro

165 Oxygen/glucose deprivation-induced injury in organotypic hippocampal slice cultures confirmed that ca

166 e of ASIC1a in AMPAR ischaemic plasticity in organotypic hippocampal slice cultures exposed to oxygen

167 ays can be regulated, in cortical neuron and organotypic hippocampal slice cultures from rat, either

168 We imaged organotypic hippocampal slice cultures of rat, in which

169 functional microglia-neuron interactions in organotypic hippocampal slice cultures, i.e., postnatal

170 ubunit-selective NMDAR antagonist, D-APV, in organotypic hippocampal slice cultures.

171 In addition, acute treatment of an organotypic hippocampal slice epilepsy model with Sema4D

172 avior after deleting Pten from neurons in an organotypic hippocampal slice network.

173 to mitochondrial and synaptic dysfunction in organotypic hippocampal slices of rats.

174 mia in vivo or oxygen-glucose deprivation in organotypic hippocampal slices or neurons, p75(NTR) is r

175 We used organotypic hippocampal slices prepared from 6-d-old Thy

176 We found that early expression of NR2A in organotypic hippocampal slices reduces the number of syn

177 ppocampus, pair recordings were performed in organotypic hippocampal slices taken from genetically mo

178 We show in rat organotypic hippocampal slices that prolonged network si

179 We therefore used chained microRNAs in rat organotypic hippocampal slices to generate a reduced bac

180 Using live-cell confocal microscopy of rat organotypic hippocampal slices, we find that enhancing n

181 Here, using mouse organotypic hippocampal slices, we show that the extrace

182 ge to neurons in dissociated cultures and in organotypic hippocampal slices.

183 Here, we used a novel organotypic human lung three-dimensional (3D) model to i

184 In addition, using organotypic human midgestation chorionic villous explant

185 In an organotypic human skin model, UVA penetration is suffici

186 els in vivo and enhances leptin signaling in organotypic hypothalamic slices.

187 well and, the more physiologically relevant, organotypic invasion assays, recapitulating the behaviou

188 ances in the design and assembly of in vitro organotypic liver and gastrointestinal (GI) models can a

189 Organotypic liver culture models for hepatotoxicity stud

190 ucing oncogene expression in single cells in organotypic mammary acini as a model to elucidate the pr

191 In this study, we used two-dimensional and organotypic melanoma culture models in combination with

192 n and maintenance of active Src signaling in organotypic microenvironments.

193 Here, we have engineered a unique organotypic model of angiogenic sprouting and neovessel

194 three-dimensional tissue culture to build an organotypic model of bronchial dysplasia.

195 Here we use an engineered organotypic model of perfused microvessels to show that

196 Here we introduce a microscale organotypic model of the human bronchiole for studying p

197 In the organotypic model, and in mice, ATRA induced quiescence

198 ect comparison between these next generation organotypic models and more traditional methods, we obse

199 ensive and resource intensive, the design of organotypic models has renewed significance.

200 creased the ability of C. albicans to invade organotypic models of the oral and esophageal mucosae un

201 nd 3-dimensional cell culture models and new organotypic models that better replicate tissue structur

202 , as important regulators of this process in organotypic models.

203 to inhibit local cancer cell invasion in 3D organotypic models.

204 CD49f(+) cells expanding in vitro underwent organotypic morphogenesis forming ductular structures an

205 xcitatory synaptic transmission in acute and organotypic neocortical slices of mice.

206 We screened 3D organotypic neoplasia with well-characterized signaling

207 this study, using oral epithelial cultures, organotypic oral mucosal constructs, and a mouse model o

208 valis within monolayers of keratinocytes and organotypic oral mucosal models.

209 Here, we create three-dimensional organotypic pancreatic cultures, named pancreatoids, com

210 Thus, organotypic pancreatoids provide a useful tool for devel

211 In organotypic preparations from the hippocampus of juvenil

212 mouse ES cells (ES-NS) and co-cultured with organotypic preparations of gut tissue consisting of the

213 Tumor cell invasion was studied using organotypic raft cultures and in vivo significance was a

214 increase HPV31b titers within the context of organotypic raft cultures compared with the level for ve

215 Our deep-sequencing analysis of organotypic raft cultures identified microRNA 145 (miR-1

216 specifically regulated by HPV16 and HPV18 in organotypic raft cultures of foreskin and vaginal kerati

217 Using organotypic raft cultures of primary human keratinocytes

218 ns to promote unscheduled DNA replication in organotypic raft cultures.

219 wed reduced viral titers following growth in organotypic raft cultures.

220 merged cultures and in a "three-dimensional" organotypic raft model of human epidermis, in part, via

221 ytes in monolayer cultures and their derived organotypic (raft) cultures, although it exhibits only a

222 In addition, HPV16-low folate-organotypic rafts contained fewer HPV16 viral particles,

223 keratinocyte monolayers and HPV16-low folate-organotypic rafts developed in physiological low folate

224 implantation of 18-day old HPV16-low folate-organotypic rafts into folate-replete immunodeficient mi

225 mic DNA when compared with HPV16-high folate-organotypic rafts.

226 g human (BC-1-Ep/SL) keratinocytes and HPV16-organotypic rafts.

227 er cells and glutamate-induced cell death in organotypic rat brain slices, suggesting similarities be

228 In organotypic rat hippocampal slices, we find that a nonph

229 Treatment of three-dimensional organotypic RDEB skin cultures with inhibitors of TGFbet

230 urthermore, AMPA stimulated NO production in organotypic retinal cultures and increased Src activity

231 and mesenchymal stem cell-derived factors in organotypic retinal explant culture and an in vivo model

232 duced phase advances of PER2::LUC rhythms in organotypic SCN cultures from GIRK2 KO mice.

233 mCry1-luc organotypic SCN slices exhibited stable circadian biolum

234 ocalized to the dermal-epidermal junction in organotypic skin culture.

235 tion, and differentiation gene expression in organotypic skin culture.

236 entiation, and migration in large numbers of organotypic skin cultures comprising epidermis and dermi

237 We used human 3-dimensional organotypic skin cultures consisting of atopic fibroblas

238 lopment by using a genetic model of RDEB and organotypic skin cultures.

239 es and in abnormal epidermal architecture in organotypic skin models recovering from UVB exposure.

240 histamine to human keratinocyte cultures and organotypic skin models reduced the expression of the di

241 Using organotypic skin models stimulated with Th2 cytokines IL

242 We used human 3-dimensional organotypic skin models with either primary keratinocyte

243 Using organotypic skin models with primary keratinocytes from

244 T cells elicited a differentiation defect in organotypic skin models, associated with reduced epiderm

245 ng bacterial growth on the three-dimensional organotypic skin models.

246 pression was determined in three-dimensional organotypic skin models.

247 h and differentiation of keratinocytes using organotypic skin raft culture model of human epidermis.

248 pigment to surrounding keratinocytes in a 3D organotypic skin reconstruct.

249 sible mechanisms were analyzed both in mouse organotypic slice culture and in rat cell culture by ind

250 This hybrid Organotypic Slice Culture Assay coupled with RT-QuIC (OS

251 screening system coupled to an ex vivo prion organotypic slice culture model to rapidly advance ratio

252 ta2*-nAChRs by nicotine either in vivo or in organotypic slice culture quickly elevates the number of

253 Using an organotypic slice culture system, we detected fragmented

254 in a chemical-induced demyelination model on organotypic slice culture, in a BDNF-dependent manner.

255 s two-photon imaging of graded potentials in organotypic slice cultures and in Drosophila.

256 and myelin synthesis was tested directly in organotypic slice cultures and OPC-neuron cocultures.

257 Spinal organotypic slice cultures from a mutant form of human s

258 s developed using z-stack images from murine organotypic slice cultures from central nervous system t

259 We employed hippocampal organotypic slice cultures from mice lacking key presyna

260 Using organotypic slice cultures from the forebrain and cerebe

261 We have used organotypic slice cultures from wild type and G93A SOD1

262 We show in vivo in rats and ex vivo in mouse organotypic slice cultures that posttraumatic GABA(A)-me

263 Circadian oscillation of mouse SCN organotypic slice cultures was monitored as PER2::LUC bi

264 iated knockdown in rat and mouse hippocampal organotypic slice cultures, a postsynaptic density-95 (P

265 odotoxin or AP5 treatment in rat hippocampal organotypic slice cultures.

266 ppocampal cell death in mechanically injured organotypic slice cultures.

267 apical dendrites of CA1 pyramidal neurons in organotypic slice cultures.

268 ngle-trial detection of action potentials in organotypic slice cultures.

269 We have developed an organotypic slice preparation of the normal portions of

270 nfocal time-lapse imaging in rat hippocampal organotypic slices and assessed the role of GluN3A-conta

271 noid 3 receptors (EP3R), breathing brainstem organotypic slices and optogenetic inhibition of EP3R(+/

272 of new therapies in human heart tissue using organotypic slices isolated from donor and end-stage fai

273 Ss were also evoked as a result of depriving organotypic slices of activity by treating them with tet

274 h3A interaction with interfering peptides in organotypic slices or in vivo induces a decrease of the

275 mpal neurons, dentate granule cells in mouse organotypic slices, and layer 2/3 pyramidal neuron in so

276 ical neurons, dentate granule cells in mouse organotypic slices, and layer 2/3 pyramidal neurons in t

277 In organotypic slices, NMDAR-dependent LTD of AMPAR excitat

278 In cultured neurons and hippocampal organotypic slices, synaptic release was reduced up to 1

279 KO hippocampal organotypic slices, which lack an intact peripheral immu

280 ing structural plasticity in rat hippocampal organotypic slices, which retain the diversity of neuron

281 specifically in intact neurons in culture or organotypic slices.

282 increased levels of apoptotic cell death in organotypic slices.

283 se studies provide insight into the basis of organotypic specificity and redundancy in RET signaling

284 on of ThT-negative, acylated SOD1 fibrils to organotypic spinal cord failed to produce the SOD1 inclu

285 In a previous study, we developed organotypic spinal cultures from an ALS mouse model expr

286 Cultivation of tumour slices required organotypic support materials and atmospheric oxygen for

287 Here, using an organotypic system that recapitulates distinct stages of

288 ct of deregulated SOX2 expression in a novel organotypic system that recreates the molecular and micr

289 pose a pathway to prevention that uses human organotypic systems that recapitulate hallmarks of aging

290 In organotypic three-dimensional cell cultures, endogenous

291 ethyl maleate-induced oxidative stress in an organotypic three-dimensional culture assay.

292 Using the mouse mammary gland, we designed organotypic three-dimensional culture models that permit

293 itulated upon Notch inhibition by DNMAML1 in organotypic three-dimensional culture, a form of human t

294 ere defined in monolayer and differentiated, organotypic three-dimensional cultures of rat epidermal

295 gulated alternative splicing (AS) targets in organotypic three-dimensional MCF-10A cell cultures that

296 In human organotypic three-dimensional skin and skin reconstituti

297 Three-dimensional organotypic tissue culture (OTC) is an innovative three-

298 r corneas (source of repellant molecules) in organotypic tissue culture both lens and cornea tissues

299 en modest success in producing hiPSC-derived organotypic tissues or organoids.

300 arget proteins in confined intercellular and organotypic tissues, with reduced steric hindrance and n